ORIGINAL_ARTICLE
Study of zoonotic bacteria in conventional laboratory mice breeding colonies
To investigate the status of conventional laboratory mice colonies for zoonotic bacteria, three mice colonies, NIH, NMRI and Balb/c strains of Razi institute were studied for five zoonotic bacteria including Pasteurella pneumotropica, Salmonella typhimurium, Salmonella enteritidis, Streptobacillus moniliformis, Streptococcus Pneumonia. Study populations were 74 mice of premature, mature and post mature ages. Same numbers of male and female animals were allocated in a random fashion from each 3 strains of mice. Two hundred and ninety six specimens of nasopharynx (n=156)),cecum (n=70) and liver (n=70) were taken from 74 animals and cultured for bacteriological tests. Results showed that none of tested specimens, infected by mentioned zoonotic bacteria, however in 78% (231/296) of mice, commonly nonpathogenic bacteria, mainly Escherichia coli, Bacillus sp, Streptococcus other than Streptobacillus moniliformis, and Entrobacter aerogenes were observed. The results of study recommend use of these animals have no human risk or research interferences for mentioned zoonotic bacteria. However like other conventional laboratory animals, precaution and personnel's safeties must be considered while using in researches where the existing bacteria may interfere the results.
https://ijvm.ut.ac.ir/article_19602_d7fa6109af11ae023e543e8eed487ffd.pdf
2009-07-01
10.22059/ijvm.2009.19602
Fatemeh
Todehdehghan
f.todehdehghan5@rvsri.ir
1
موسسه تحقیقات واکسن و سرم سازی رازی
AUTHOR
Mohammad Hassan
Motedayen
1todehdehghan@rvsri.ir
2
موسسه تحقیقات واکسن و سرم سازی رازی
AUTHOR
Parviz
Tajik
tajikp@ut.ac.ir
3
دانشکده دامپزشکی
AUTHOR
Sohila
Moradi Bidhendi
2todehdehghan@rvsri.ir
4
موسسه تحقیقات واکسن و سرم سازی رازی
AUTHOR
ORIGINAL_ARTICLE
Serodiagnosis of leptospirosis in cattle in north of Iran, Gilan
In order to investigate the seroprevalence of leptospirosis in traditional dairy farms, a serological study was conducted in Gilan province. For this study,, a total of 205 serum samples were randomly collected from nine districts of this province. All serum samples were serologically tested by the standard MAT using live antigens representing the following Leptospira interrogans serovars: Gripptyphosa, Icterohaemorrhagiae, Hardjo, Canicola, Ballum and Pomona. The lowest dilution that each serum was considered positive was 1:100. The results of this study showed that 53 (25.8%) animals had a positive reaction against one or more serovars. The most prevalent Leptospira serovar was Canicola representing representing 24 (11.7%) samples, and the least prevalent Leptospira serovars were Icterohaemorrhagiae representing 1 (0.5%) sample and Hardjo, 2 (1%) samples. The most prevalent reciprocal titer was 100 and the highest titer was 400. The results of this study may support that cows may have a role in maintaining Canicola serovar. As mostany of the visited herds had at least one unvaccinated dog for guarding the herds, it has beenis concluded that the high prevalence of Canicola serogroup can be associated with close contact between dogs and cows. In addition, infected cows are a potential zoonotic risk to farmers, milkers, abattoir workers and meat inspectors, which previously has not been seriously considered.
https://ijvm.ut.ac.ir/article_19603_04a088c7766e7284855a7d4a71175944.pdf
2009-07-01
10.22059/ijvm.2009.19603
GholamReza
Abdollahpour
greza@ut.ac.ir
1
دانشکده دامپزشکی
AUTHOR
Seyyedeh Tooba
Shafighi
1greza@ut.ac.ir
2
دانشکده دامپزشکی
AUTHOR
Saeid
Sattari TAbrizi
2greza@ut.ac.ir
3
دانشکده دامپزشکی
AUTHOR
ORIGINAL_ARTICLE
Laboratory evaluation of three strains of the entomopathogenic fungus Metarhizium anisopliae for controlling Hyalomma anatolicum anatolicum and Haemaphysalis punctata
Hyalomma anatolicum anatolicum and Haemaphysalis punctata larval ticks were shown to be susceptible to different strains of entomopathogenic fungi Metarhizium anisopliae under laboratory conditions. To determine the susceptibility of H. anatolicum anatolicum and H. punctata to M. anisopliae, two suspensions of conidia were used (103 and 104 spores/ml). The treatments were conducted by immersing larval stage (at least 20 larvae/Petri dish) of H.anatolicum anatolicum and H.punctata in the spore suspension for 30 sec, followed by transferring to Petri dish containing moist filter paper. Control larval ticks were only immersed in 0.05% aqueous Tween 80. All treated and untreated ticks were observed by day interval up to day 18 to detect dead ticks and signs of mycosis. Comparative results of bioassays using three different fungal strains showed that all strains of M. anisopliae were highly pathogenic against two tick species used. The higher mortality rate was seen with strain of 689 when used in H. punctata and 685 in H. anatolicum anatolicum in comparison with the other strains(p
https://ijvm.ut.ac.ir/article_19604_46300280f3bb037325427bd21788dd91.pdf
2009-07-01
10.22059/ijvm.2009.19604
Mosa
Tavassoli
m.tavassoli2@mail.urmia.ac.ir
1
دانشکده دامپزشکی
AUTHOR
Abdol Ghaffar
Ownag
1m.tavassoli2@mail.urmia.ac.ir
2
دانشکده دامپزشکی
AUTHOR
RuGhayeh
Meamari
2m.tavassoli2@mail.urmia.ac.ir
3
بخش خصوصی
AUTHOR
Salaheddin
Rahmani
3m.tavassoli2@mail.urmia.ac.ir
4
بخش خصوصی
AUTHOR
Karim
Mardani
4m.tavassoli@mail.urmia.ac.ir
5
دانشکده دامپزشکی
AUTHOR
Taregh
Butt
5m.tavassoli@mail.urmia.ac.ir
6
AUTHOR
ORIGINAL_ARTICLE
Effects of curcumin, morphine and naloxone on the experimentally-induced paw edema in rats
https://ijvm.ut.ac.ir/article_19605_997a48a2fa8f068133d910a8b45aa4e3.pdf
2009-07-01
10.22059/ijvm.2009.19605
Esmaeil
Tamaddonfard
e_tamaddonfard3@mail-urmia.ac.ir
1
دانشکده دامپزشکی
AUTHOR
Hossein
Tajik
1tamaddonfard@mail-urmia.ac.ir
2
دانشکده دامپزشکی
AUTHOR
Nasrin
Hamzeh gooshchi
2tamaddonfard@mail-urmia.ac.ir
3
دانشکده دامپزشکی
AUTHOR
ORIGINAL_ARTICLE
Evaluation of serum and milk haptoglobin in some inflammatory diseases of cattle
This study was designed to evaluate serum and milk haptoglobin (Hp) as an inflammatory indicators for the diagnosis of diseases in cattle in Fars province of Iran. Blood samples were collected from the coccygeal vein of 20 clinically healthy cattle and 100 cows with various inflammatory diseases. There were significant differences in serum and milk Hp between clinically healthy cattle and diseased cows (p
https://ijvm.ut.ac.ir/article_19606_2586738628ad782f89e4ee49e89d4a2c.pdf
2009-07-01
10.22059/ijvm.2009.19606
Ameneh
Khoshvaghti
1nazifi@shirazu.ac.ir
1
دانشگاه آزاد
AUTHOR
Saied
Nazifi
nazifi2@shirazu.ac.ir
2
دانشکده دامپزشکی
AUTHOR
Amir
Vafafar
2nazifi@shirazu.ac.ir
3
دانشگاه آزاد
AUTHOR
Hamid reza
Gheisari
3nazifi@shirazu.ac.ir
4
دانشکده دامپزشکی
AUTHOR
ORIGINAL_ARTICLE
Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins
Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion, immunoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion and immunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of mammalian species. Anti-camel antibodies bound camel IgG1, IgG2, IgG3 subclasses like the subclasses of sheep, but no reactivity to all subclasses chains in cattle and horse antibodies were observed. Polyclonal anti-camel IgY was specifically light chain reactive. Overall, the potential of using egg yolk immunoglobulins as a convenient source of anti antibodies to camel immunoglobulins was demonstrated.
https://ijvm.ut.ac.ir/article_19607_7d6b01229e83471ca54fa84e0db120a5.pdf
2009-07-01
10.22059/ijvm.2009.19607
Gholam Reza
Nikbakht Brujeni
nikbakht12@ut.ac.ir
1
دانشکده دامپزشکی
AUTHOR
Saeid
Tabaabaei
1nikbakht2@ut.ac.ir
2
دانشکده دامپزشکی
AUTHOR
Mahmood
Khormali
2nikbakht@ut.ac.ir
3
دانشکده دامپزشکی
AUTHOR
Iraj
Ashrafi
3nikbakht@ut.ac.ir
4
دانشکده دامپزشکی
AUTHOR
ORIGINAL_ARTICLE
Identification of Salmonella spp. and Salmonella typhimurium by a multiplex PCR-based assay from poultry carcasses in Mashhad- Iran
Poultry meat has been identified as one of the principal foodborne sources of Salmonella. In this preliminary study the prevalence of Salmonella spp. and its typhimurium serovar contamination of broiler carcasses, were determined. Using the rinse test method, numbers of 60 samples, representing 20 broiler flocks, were collected from poultry carcasses after the chilling stage in the processing line at a commercial broiler slaughtering facility in Mashhad, Iran. The presence of Salmonella spp and Salmonella typhimurium in collected samples were assessed by performing the pre-enrichment and enrichment culture, followed by multiplex-PCR assay. The primers were selected from the invA and fliC genes, specific for the detection of Salmonella spp. and Salmonella typhimurium, respectively. In this study 8.3% and 1.6% of poultry carcasses were found to be contaminated with Salmonella spp and Salmonella typhimurium respectively. In order to provide a more accurate profile of the prevalence of Salmonella spp and Salmonella typhimurium in broiler carcasses, it is pertinent to use multiplex -PCR method that could be considered as an appropriate alternative to conventional culture method.
https://ijvm.ut.ac.ir/article_19608_9460ec87a65d9ff9a03ffc0e6c1a59c8.pdf
2009-07-01
10.22059/ijvm.2009.19608
Abdollah
Jamshidi
ajamshid@ferdowsi.um.ac.ir
1
دانشکده دامپزشکی
AUTHOR
Mohammad Reza
Bassami
1ajamshid@ferdowsi.um.ac.ir
2
دانشکده دامپزشکی
AUTHOR
Samira
Afshari Nic
2ajamshid@ferdowsi.um.ac.ir
3
دانشکده دامپزشکی
AUTHOR
ORIGINAL_ARTICLE
Anti-cancer effect of ICD-85(venom derived peptides) on MDA-MB231 cell line (in vitro) and experimental mice with breast cancer (in vivo)
Breast cancer has become common in developing and developed countries. Alarming increase in this disease as a leading cause of death in women is a concern of all. Despite the significant improvements in the management of breast cancer, the survival rate is not more than 20% - 25%. For this study, MDA-MB231 cell line was used and the effect of ICD-85 (biologically active peptides from venomous animals) was assayed by measuring the activity of the cytosolic enzyme lactate dehydrogenase(LDH) released into the culture medium after membrane legions. Morphological changes of cells were checked in control and cells incubated with ICD-85 as chemo preventive agent. Results showing in test groups - incubation with 10mg/ml dose of ICD-85 had decreased cytoplasmic branch. Some cells were ruptured and lost the continuity of their surrounding membranes, and number of cells was decreased. On the other hand, when ICD-85 was used as an anticancer treatment of mice with breast cancer, it was shown that ICD-85 can prevent the growth of breast tumor, increase the life duration of experimental mice and inhibit angiogenesis in breast tumor as compared with control group. It seems that ICD-85 acts at the membrane level and can prevent the cell growth as well as producing apoptosis (cell suicide) for breast cancer cells.
https://ijvm.ut.ac.ir/article_19609_bd2a0d7a394cc3a15fbc17bff9ce7da2.pdf
2009-07-01
10.22059/ijvm.2009.19609
85ICD-
Mohammad Kazem
Koohi
1abbas.zare8@gmail.com
1
دانشکده دامپزشکی
AUTHOR
Abbas
Zare Mirakabadi
abbas.zare8@gmail.com
2
موسسه تحقیقات واکسن رازی
AUTHOR
Mojtaba
Moharrami
2abbas.zare8@gmail.com
3
موسسه تحقیقات واکسن رازی
AUTHOR
Mohammad Hassan
Hablolvarid
3abbas.zare8@gmail.com
4
دانشکده دامپزشکی
AUTHOR
ORIGINAL_ARTICLE
Electrocardiographic manifestation of digoxin toxicity in a Pomeranian dog
This case report describes several electrocardiographic findings in an 8-years old female Pomeranian dog that was referred with a history of digoxin overdose. Electrocardiographic abnormalities detected in this case could be classified as primary electrocardiographic disturbances, reflecting cardiac chamber enlargement and secondary ECG changes, attributed to digoxin toxicity. ECG manifestations of digoxin toxicity such as first and second degree AV blocks completely disappeared following digoxin withdrawal. Therefore, veterinarians should be aware of the possibility of digoxin toxicity due to its narrow therapeutic margins. The purpose of this report is to increase awareness of small animal practitioners about adverse cardiotoxic effects of cardiac glycosides. It also highlights the significance of electrocardiographic monitoring for diagnosis and management of digoxin induced cardiac arrhythmias and conduction disturbances in small animal patients
https://ijvm.ut.ac.ir/article_19610_55259189a740d5ba75665a554a2822bc.pdf
2009-07-01
10.22059/ijvm.2009.19610
Abdol Ali
Malmasi
malmasia2@vetmed.ut.ac.ir
1
دانشکده دامپزشکی
AUTHOR
Masood
Selk Ghaffari
1malmasia@vetmed.ut.ac.ir
2
دانشگاه آزاد اسلامی
AUTHOR
Sarang
Soroori
2malmasia@vetmed.ut.ac.ir
3
دانشکده دامپزشکی
AUTHOR
ORIGINAL_ARTICLE
Growth and toxigenesis behavior of Clostridium botulinum type E in Persian sturgeon (Acipenser persicus) Caviar prepared with various preservatives
Growth behavior of Clostridium botulinum type E beluga was studied in Acipenser persicus granular caviar treated with 5% NaCl, 5%NaCl plus 0.3% boric acid and 0.4%borax and 5%NaCl plus 0.15% methyl paraben incubated at temperatures -2, 5 and 15 °C for 224 days. The initial number of inoculated bacterial spore was 5.1×104 cfu/g caviar in each treated sample. The number of bacterial spores in samples treated with 5% NaCl changed to 3.59 × 104, 1.02×105 and 9.9×105 cfu/g at -2 °C, 5 °C and 15 °C, respectively, while those samples treated with 5%NaCl plus 0.3% boric acid and 0.4%borax changed to 1.56×104, 3.65×104 and 9.22×104 cfu/g, respectively. Also, number of bacterial spores in samples treated with 5% NaCl plus 0.15% methyl paraben changed to 1×104, 2.86×104 and 3.56×104 cfu/g at the above storage temperatures, respectively. Fourteen days post-inoculation, toxin production was positive in samples treated with 5% NaCl incubated at 5 °C and 15 °C, while those samples treated with 5%NaCl plus 0.3% boric acid and 0.4% borax were positive for toxin production only at 15 °C. Toxin production was negative in samples treated with 5%NaCl plus 0.15% methyl paraben incubated at all three above mentioned temperatures. These data showed that 5%NaCl plus methyl paraben had more inhibitory effect than boric, borax and NaCl. Also, such inhibitory effects can be increased at lower storage temperature of -2°C. Therefore, due to restricted usage of boric acid and borax in granular caviar, use of methyl paraben can be recommended as a safe preservative with a strong antimicrobial effect at caviar pH.
https://ijvm.ut.ac.ir/article_19611_1885c19d81f1104cecd66bb1e4387a1c.pdf
2009-07-01
10.22059/ijvm.2009.19611
.A
Salmani
salmani_j@yahoo.com
1
پژوهشکده اکولوژی آبزیان دریای خزر
AUTHOR
.R
Safari
1salmani_j@yahoo.com
2
دانشکده دامپزشکی
AUTHOR
.M
Soltani
2salmani_j@yahoo.com
3
داتشکده دامپزشکی دانشگاه تهران
AUTHOR
.H.R
Tavakoli
3salmani_j@yahoo.com
4
دانشکده بهداشت دانشگاه علوم پزشکی بقیه ا...
AUTHOR