ORIGINAL_ARTICLE
Clinical and Radiological Evaluation of Transplanted Fresh Ear Cartilage Impregnated With the Mesenchymal Cells & PRP in Treatment of Growth Plate Injury in Lamb
Background: Growth plate is responsible for bone elongation and its injury could result in severe orthopedic problems. OBJECTIVES: This study was aimed to investigate the radiographic changes after transplanting the fresh autogenous ear cartilage impregnated with PRP and MSc in treatment of growth plate injury in lamb. METHODS: Cranial half (½) in length in full depth of the right hind limb growth plate was resected with bone oscillator and was transplanted with autogenous fresh harvested ear cartilage at the time of surgery in all of the 15 male lambs. Subsequently they were divided into 3 subgroups of 5 lambs in each group, control, PRP and MSCs. Radiographs were obtained from operated limbs at surgery time and 60 post-operative. RESULTS: Clinically, all lambs showed same lameness degree and mostly apparent after cast removal but gradually improved in the treated group which showed normal weight bearing after one month. Early physeal closure was seen in growth plate injured site only in control group without limb shortening or angular deformity. Lambs with MSCs showed mild bone bridge formation (1.4b ± 0.4b) as compared with PRP group (1.8 ± 0.37ab) and control group (2.8 ± 0.2 a) (P<0.05).On 60 days postoperatively control group showed significant mineralization (by 2.8±0.2a) while less mineralization was detected in MSCs and PRP groups (by 1.4± 0.4b and 2.4± o.4 ab). CONCLUSIONS: This study showed that MSCs & PRP can quite effective in preventing bone bridge formation and mineralization besides convergence of the transplanted tissue into growth plate cartilage. Keywords:
https://ijvm.ut.ac.ir/article_68585_ebea4e298ab07a025394c2e8ee6b65d2.pdf
2018-10-01
283
291
10.22059/ijvm.2018.257195.1004897
Cartilage
Growth plate
Mesenchymal cell
PRP
Radiography
Alaa
AlDirawi
ibrahimalaa348@yahoo.com
1
Instructor at the University of Basra, Basra, Iraq. Post–Graduate Student at the Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Davood
Sharifi
dsharifi@ut.ac.ir
2
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran
LEAD_AUTHOR
Sarang
Soroori
soroori@ut.ac.ir
3
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran
AUTHOR
Saied
Bokaie
sbokaie@ut.ac.ir
4
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine University of Tehran, Tehran, Iran
AUTHOR
Roshanak
Mokhtari
r.mokhtari@ut.ac.ir
5
Department of surgery and Radiology, faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
AUTHOR
Alireza
Bashiri
alirezabashiri@ut.ac.ir
6
Department of Surgery and Radiology, Faculty of Veterinary Medicine .University of Tehran. Tehran. Iran.
AUTHOR
Hosein
Shad
hshad55@yahoo.com
7
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine .University of Tehran. Tehran. Iran
AUTHOR
Rouhoulah
Esanejad
hissanejhad@yahoo.com
8
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine .University of Tehran. Tehran. Iran
AUTHOR
Davoud
Faskhoudi
davoodfaskhoodi@gmail.com
9
Department of clinical Sciences, Faculty of Veterinary Medicine, the University of Tehran, Tehran, Iran.
AUTHOR
. Apte, S.S., Kenwright, J. (1994) Physeal distraction and cell proliferation in the growth plate. J Bone Joint Surg Br. 76: 837-843.
1
Aston, E.A., Bentley, G. (1986) Repair of articular surface by allograft of articular and growth plate cartilage. J Bone Joint Surg [Br]. 68-B: 29-34.
2
Basener, C.J., Mehlman, C.T., DiPasquale, T.G. (2009) Growth disturbance after distal femoral growth plate fractures in children: a meta-analysis. J Orthop Trauma. 23: 663-667.
3
Borsa, J.J., Peterson, H.A., Ehman, R.L. (1996) MR imaging of physeal bars. Radiology. 199: 683-687.
4
Brittberg, M., Lindahl, A., Nilsson, A. (1994) Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. New Engl J Med. 331: 889-95.
5
Brittberg, M., Nilsson, A., Lindahl, A., Ohlsson, C., Peterson, L. (1996) Rabbit articular cartilage defects treated with autologous cultured chondrocytes. Clin Orthop. 326: 270-83.
6
Caplan, A.I. (1991) Mesenchymal stem cells. J Orthop Res. 9: 641-50.
7
Caplan, A.I., Elyaderani, M., Mochizuki, Y., Wakitani, S., Goldberg, V.M. (1997) Principles of cartilage repair and regeneration. Clin Orthop. 342: 254-69.
8
Cotran, R.S., Kumar, V., Collins, T. (1999) Cellular pathology II. Adaptations, intracellular accumulations, and cell aging. In: Robbins Pathological Basis of Disease. (6th ed.) Philadelphia: W B Saunders, USA. p. 46-7.
9
Dabash, Sh., Prabhakar, G., Potter, E., Thabet, A.M., Abdelgawad, A., Heinrich, S. (2018) Management of growth arrest: Current practice and future directions. J Clin Orthop Trauma. 9, S. 1: S58-S66.
10
Davis, K.W. (2010) Imaging pediatric sports injuries: lower extremity. Radiol Clin North Am. 48: 1213-1235.
11
Dimeglio, A. (2001) Growth in pediatric orthopaedics. J Pediatr Orthop. 21: 549-555.
12
Goldberg, V.M., Caplan, A.I. (1999) Biological restoration of articular surfaces. AAOS ICL. 48: 623-7.
13
Jawetz, S.T., Shah, P., Potter, H.G. (2015) Imaging of physeal injury. Sports Health. 7(2): 142–153.
14
Lew ,C., Schon, Nicholas Gill, Margaret Thrope, Joel Davis, Joshua Nadaud, Jooyoung Kim,Jeremy Molligan, Zijun Zhang.(2016) Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats. J Translat Med. 12: 110-125.
15
Paterno, M.V., Taylor-Haas, J.A., Myer, G.D., Hewett, T.E. (2013) Prevention of overuse sports injuries in the young athlete. Orthop Clin North Am. 44: 553.
16
Pauline, P.o., Yee, Lui. (2015) Stem cell technology for tendon regeneration: current status, challenges, and future research directions Stem Cells and Cloning: Advances and Applications. 8: 163-174.
17
Peter, A. M., Everts, Johannes, T. A., Knape, Gernot –Weibrich, M. D., Jacques, P. A., Mschonberger. M.D., ohannes, Hoffmann,. Eddy. P., Overdevest., Henk A.M., Box Andre -van Zundert. (2006) Platelet Rich Plasma and Platelet Gel: Review. JECT. J Extra Corpor Technol. 38: 174-187.
18
Noguchi, T., Oka, M., Fujino, M., Neo. M.,Yamamuro, T.I. (1994) Repair of osteochondral defects with grafts of cultured chondrocyte: comparison of allograft and isografts. Clin Orthop. 302: 251.
19
Salma, Chaudhury., Mauricio, de- La., Lama.Ronald, S., Adler.Lawrence, V., Gulotta.Brendan., Skonieezki. Anthony., Chang. Peter Moley., Frank Cordasco, J.o., Hannafin., Stephen- Fealy. (2013) Platelet-rich plasma for the treatment of lateral epicondilitis: sonographic assessment of tendon morphology and vascularity (Pilot study). Skeletal Radiol. 42: 91-97.
20
Shari, T., Jawetz, M.D., Parina, H., Shah, M.S., Hollis, G., Potter, M.D. (2015) Imaging of Physeal Injury. Sports Health. 2015 Mar. 7(2): 142–153.
21
Stracciolini, A., Casciano, R., Levey-Friedman, H., Stein, C.J., Meehan, W.P., Micheli, L.J. (2014) Pediatric sports injuries: a comparison of males versus females. Am J Sports Med. 42: 965-972.
22
Takahashi, S., Oka, M., Kotoura ,Y., Yamamuro, T. (1995) Autogenous call osseous grafts for the repair of osteochondral defects. J Bone Joint Surg [Br]. 77-B: 194-204.
23
Wakitani, S., Kimura, T., Hirooka, A. (1989) Repair of rabbit articular surface with allograft chondrocyte embedded in collagen gel. J Bone Joint Surg [Br]. 71-B: 74-80.
24
Wakitani, S., Goto, T., Pineda, S.J. (1994) Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg [Am]. 76-A: 579-92.
25
ORIGINAL_ARTICLE
Investigation of the Relationship between Ultrasonographic Findings and Analyses of Bladder Contents in Cats
Background: Echoes are frequently observed in cat bladder contents through ultrasonography and often mentioned as incidental findings. No comprehensive study has been conducted so far on the precise echoes nature in the bladder contents ultrasonography in cats. OBJECTIVES: The purpose of this study is to provide an accurate description of the cat bladder contents echoes and to specify the relationship between ultrasonographic findings and urinalyses. METHODS: 30 adult DSH cats were investigated. Bladder ultrasonography was performed. The numbers of echoes in the bladder were qualitatively divided into four classes. At least 5 ml of urine was collected with cystocentesis. Urinalysis was performed on the urine sediment. The statistical analyses were done. RESULTS: Echoes were observed in 19 cases. The echoes were suspended in 17 of the cases, in those cases they were observed and clumped in 2 cases. Of the 19 cases where echoes had been observed in the bladder contents, 9 included oil droplets, 2 included struvite crystals and 4 included hematuria as reported in urinalysis. For 6 of the cats with echoes in the bladder contents, oil droplets, RBCs, and crystals were not reported in urinalysis. The number of echoes observed in the bladder in presence of RBCs, oil droplets, crystals, and leucocytes was not statistically significant (P>0.05). CONCLUSIONS: Based on this study, most of the echoes observed in the ultrasonography of cats’ bladder contents can be associated with the presence of oil droplets in the urine, which is in accordance with previous studies.
https://ijvm.ut.ac.ir/article_68608_e96f83be6a46811f5581c723bc0c7ffb.pdf
2018-10-01
293
303
10.22059/ijvm.2018.263125.1004914
Cat
Echoes
Ultrasound
Urinalysis
Urinary bladder _________________________________________________________________________________________
Somayeh
Davudypoor
davudypoor@ut.ac.ir
1
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Alireza
Vajhi
avajhi@ut.ac.ir
2
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
Mohammad
Molazem
mmolazem@ut.ac.ir
3
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Sarang
Soroori
soroori@ut.ac.ir
4
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Majid
Masoudifard
mmfard@ut.ac.ir
5
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Omid
Zehtabvar
ozehtabvar@ut.ac.ir
6
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
AUTHOR
Adin, C.A., Chew, D.J., Heng, H.G., Townsend, K.T., Karnik, K. (2011) Bladder inversion and secondary hematuria in a 6-month-old domestic shorthair cat. JAVAM. 239: 370-373.
1
Aranovich, D., Kaminsky, O., Zigerman, E., Yussim, E., Greif, F. (2008) Should echogenic material in the urinary bladder noticed on FAST preclude urinary catheter insertion in a trauma patient until further evaluation. Eur J Trauma Emerg Surg. 6: 592-594.
2
Barr, F., Gaschen, L. (2011) Manual of canine and feline ultrasonography (BSAVA). (1st ed.) Wiley Blackwell. Iowa, USA. p.159-163.
3
Baumann, J.M., Affolter, B. (2014) From crystalluria to kidney stones, some physiocochemical aspects of calcium nephrolithiasis.World J Nephrol. 3: 256-267.
4
Bell, E.T., Lulich, J.P. (2015) Marked struvite crystalluria and its association with lower urinary tract signs in a cat with feline idiopathic cystitis. Aust Vet J. 93: 332-335.
5
Brabson, T. L., Bloch, C.P., Johnson, J.A. (2015) Correlation of gross urine color with diagnostic findings in male cats with naturally occurring urethral obstruction. J Feline Med Surg. 17: 453-457.
6
Cherbinsky, O., Westropp, J., Tinga, S., Joners, B., Pollard, R. (2010) Ultrasonographic features of grass awns in the urinary bladder.Vet Radiol Ultrasound. 51. 462-465.
7
Cooper, E.S. (2015) Controversies in the management of feline urethral obstruction. J Vet Emerg Crit Care. 25: 130-137.
8
Dennis, R., Kirberger, R.M., Barr, F., Wrigley, R.H. (2010) Handbook of small animal radiology and ultrasound. (2nd ed.) Elsevier.Philadelphia, USA. p. 310-317.
9
Eggersdottir, A.V., Lund, H.S., Krontveit, R., Sorum, H. (2007) Bacteriuria in cats with feline lower urinary tract disease: a clinical study of 134 cases in Norway. J Feline Med Surg. 9: 458-465.
10
Lobetti, R.G., Goldin, J.P. (1998) Emphysematous cystitis and bladder trigone diverticulum in a dog. J Small Anim Pract. 39: 144-147.
11
Mattoon, J.S., Nyland, T.G. (2015) Small animal diagnostic ultrasound. (3rd ed.) Saunders Company. Philadelphia, USA. p. 590-600.
12
Nevins, J.R., Mai, W., Thomas, E. (2015) Associations between ultrasound and clinical findings in 87 cats with urethral obstruction. Vet Radiol Ultrasound. 56: 439-447.
13
O’Brien, R., Barr, F.J. (2009) Manual of canine and feline abdominal imaging (BSAVA). (1st ed.) Wiley Blackwell. Iowa, USA. p.210.
14
Penninck, D., D Anjou, M.A. (2015) Atlas of small animal ultrasonography. (2nd ed.) Wiley Blackwell. Iowa, USA. p. 363-385.
15
Sislak, M.D., Spaulding, K.A., Zoran, D.L., Bauer, J.E., Thompson, J.A. (2014) Ultrasonographic characteristics of lipiduria in clinically normal cats. Vet Radiol Ultrasound. 55L. 2: 195-201.
16
Thrall, D.E. (2013) Textbook of Veterinary Diagnostic Radiology. (6th ed.) Saunders Company. Philadelphia, USA. p. 735-737.
17
Thrall, D.E. (2018) Textbook of Veterinary Diagnostic Radiology. (7th ed.) Saunders Company. Philadelphia, USA. p. 846-864.
18
Vila, A., Movilla, R., Castro, J., Mallol, C., Novellas, R., Espada, Y., Roura, X. (2018) Successful medical management of pseudomembranous cystitis in three cats with lower urinary tract obstruction. Aust Vet J. 96: 1-2, 33-38.
19
Voros, K., Wladar, S., Marsi, A. (1997) Ultrasonographic study of feline lower urinary tract diseases: 32 cases. Acta Vet Hung. 45: 387–395.
20
Widmer, W.R., Biller, D.S., Adams, L.G. (2004) Ultrasonography of the urinary tract in small animals. JAVMA. 225: 46-54.
21
Yeager, A.E., Anderson, W.I. (1989) Study of association between histologic features and echogenicity of architecturally normal cat kidneys. Am J Vet Res. 50: 860–863.
22
ORIGINAL_ARTICLE
Computed Tomographic Evaluation of Tooth Growth in Response to Diode Laser Irradiation in Rabbits
Background: While many dental procedures induce pulpal damage by increase the temperature, this study was established to evaluate the thermally effect of different laser methods on pulp, oral soft and hard tissues. Objectives: In this study, laser irradiation applied to measure how increased in power output can influence on dental pulp by using CT images. Methods: Ten adult New Zealand white rabbits were divided into 2 groups receiving 3 and 5 watt for 15 second respectively on left side cheek teeth after shortening of third upper and first lower premolars. Right cheek teeth trimmed as well and served as control. CT scans were taken immediately after lasing and repeated every week for one month. Results: The statistical analysis of dental measurements shows that teeth length of right and left third upper premolar was significantly different during time (P<0.012). While in first lower premolar there was no significant differences between right and left sides (P=0.338). In every week CT, group one showed no differences in right and left side in comparison to group two (P>0.05). Only third upper premolar in fourth week showed a significant difference between two groups (P=0.047). Conclusions: laser radiation could influence on tooth growth in both groups. This study revealed that the higher laser power would have been more effective on reduction of growth rate of teeth. In addition, 3D CT scans could be an appropriate tools for dental growth investigation.
https://ijvm.ut.ac.ir/article_68660_834c275e3ddab64fd1ebc8062f8a2cbd.pdf
2018-10-01
305
312
10.22059/ijvm.2018.264603.1004918
CT images
Dental growth
Laser irradiation
Rabbit
Thermal damage
Moloud
Davoodi
mdavoodi@ut.ac.ir
1
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Amir
Rostami
arostami@ut.ac.ir
2
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
Azin
Tavakoli
azin.tavakoli@gmail.com
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran
AUTHOR
Sarang
Soroori
soroori@ut.ac.ir
4
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Alirezaا
Bahonar
abahonar@ut.ac.ir
5
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Arash
Rahimi
dr.arash_dds@yahoo.com
6
Department of Biophysics, Islamic Azad University Science and Research Branch, Tehran, Iran
AUTHOR
Baldissara, P., Catapano, S., Scotti, R. (1997) Clinical and histological evaluation of thermal injury thresholds in human teeth: preliminary study. J Oral Rehabil. 24: 791-801. DIO: https://doi.org/10.1111/j.1365-2842.1997.tb00278.x. PMID: 9426160.
1
Bohmer, E. (2015) Dentistry in rabbits and rodents. (1st ed.) John Wiley & Sons Ltd. West Sussex, UK. p. 5-20. DOI: https://doi.org/10.1002/9781118802557.
2
David, C. M., Gupta, P. (2015) Laser in dentistry: A review. Int J Adv Health Sci. 2: 7-13.
3
Gaspar, L., Kasler, M., Orosz, M. (1991) Effect and application of CO2 laser beam angle of incidence on different parts of the oral cavity using oral cavity endoscopes. J Clin Laser Med Surg. 9: 209-213. DOI: https:/doi.org/10.1089/clm.1991.9.219. PMID: 10149459.
4
Kivanc, B. H., Arisu, H. D., Ulusoy, O. I. A., Saglam, B. C., Gorgul, G. (2012) Effect of light-activated bleaching on pulp chamber temperature rise: An in vitro study. Aust Endod J. 38: 76–79. DOI: https://doi.org/10.1111/j.1747-4477.2010.00271.x. PMID: 22827820.
5
Klunboot, U., Arayathanitkul, K., Chitaree, R., Emarat, N. (2012) The temperature effects of diode laser on pulpal tissues for the teeth whitening treatment. Procedia Eng. 32: 722–726. https://doi.org/10.1016/j.proeng.2012.02.003.
6
Kreisler, M., Al Haj, H., D’Hoedt, B. (2003) Temperature changes induced by 809-nm GaAlAs laser at the implant–bone interface during simulated surface decontamination. Clin Oral Implant Res. 14: 91–96. DOI: https://doi.org/10.1034/j.1600-0501.2003.140112.x. PMID: 12562370.
7
Kwon, S. J., Park, Y. J., Jun, S. H., Ahn, J. S., Lee, I. B., Cho, B. H., Son, H.H., Seo, D.G. (2013) Thermal irritation of teeth during dental treatment procedures. Restor Dent Endod. 38: 105–112. DOI: https://doi.org/10.5395/rde.2013.38.3.105. PMID: 24010075.
8
Miserendino, L. J., Levy, G. C., Abt, E., Rizoiu, L. M. (1994) Histologic effects of a thermally cooled Nd: YAG laser on the dental pulp and supporting structures of rabbit teeth. Oral Surg Oral Med Oral Path. 76: 93-100. DOI: https://doi.org/10.1016/0030-4220(94)90123-6. PMID: 8078669.
9
Müller, J., Clauss, M., Codron, D., Schulz, E., Hummel, J., Fortelius, M., Kircher, P., Hatt, J.M. (2014) Growth and Wear of Incisor and Cheek Teeth in Domestic Rabbits (Oryctolagus cuniculus) Fed Diets of Different Abrasiveness. J Exp Zool. 321A:283–298. doi: https://doi.org/10.1002/jez.1864. PMID: 24700486.
10
Riggs, G. G., Cissell, D. D., Arzi, B., Hatcher, D. C., Kass, P. H., Zhen, A., Verstraete, F.J. (2017) Clinical application of cone beam computed tomography of the rabbit head: part 2- dental disease. Front Vet Sci. 4: 5. DOI: https://doi.org/10.3389/fvets.2017.00005. PMID: 28194401.
11
Sulieman, M., Rees, J. S., Addy, M. (2006) Surface and pulp chamber temperature rises during tooth bleaching using a diode laser: a study in vitro, Br Dent J. 200: 631–634. DOI: https://doi.org/10.1038/sj.bdj.4813644. PMID: 16767142.
12
Tate,Y., Yoshiba, K., Yoshiba, N., Iwaku, M., Okiji, T., Ohshima, H. (2006) Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry. Eur J Oral Sci. 114: 50–57. DOI: https://doi.org/10.1111/j.1600-0722.2006.00261.x. PMID: 16460341.
13
White, J. M., Fagan, M.C., Goodis, H.E. (1994) Intrapulpal temperatures during pulsed Nd. YAG laser treatment of dentin, in vitro. J Periodontol. 65: 255–259. DOI: https://doi.org/10.1902/jop.1994.65.3.255. PMID: 8164119.
14
Wyss, F., Clauss, M., Kircher, P., Geyer, H., Rechenberg, B., Hatt, J. M. (2016) Measuring Rabbit (Oryctolagus cuniculus) Tooth Growth and Eruption by Fluorescence Markers and Bur Marks. J Vet Dent. 33: 39-46. DOI: https://doi.org/10.1177/0898756416640956. PMID: 27487654
15
Zach, L., Cohen, G. (1965) Pulp response to externally applied heat. Oral Surg Oral Med Oral Path. 19: 515-530. DOI: https://doi.org/10.1016/0030-4220(65)90015-0. PMID: 14263662.
16
ORIGINAL_ARTICLE
BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry
Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which were raised in various farms in the Garmsar district and surrounding areas and were brought to the local abattoir for slaughtering between April and September 2016, were examined. RESULTS: Macroscopic pneumonia findings were detected in different lobes particularly in the apical and cardiac lobes of the lungs of259 sheep (6.35%). The rates of mild, moderate and severe consolidations observed in the pneumonic lungs were 59.8%, 26.3 % and 11.6 %, respectively. Pneumonias were microscopically classified in sheep as interstitial pneumonia (49.8%), suppurative bronchopneumonia (15.7%), bronchointerstitial pneumonia (11.1 %), and parasitic pneumonia (14.3%). A total of 220 pneumonic lungs, excluding parasitic pneumonia, examination with immunohistochemistry (IH) in terms of BHV1 antigen, were considered. BHV1 antigen was determined to be 8.63 % by the immunohistochemistry (IHC) method. CONCLUSIONS: In conclusion, the presence of viral antigen in lung tissues of sheep may indicate that natural pneumonia may be induced by BHV1 or possibly other species-specific herpesviruses. Moreover, it is suggested that sheep might have a role in the transmission of this virus to cattle.
https://ijvm.ut.ac.ir/article_68554_c1882a67da80659d741c532de35a01a7.pdf
2018-10-01
313
321
10.22059/ijvm.2018.233674.1004890
BHV1
Immunohistochemistry
Lung
Pneumonia
sheep
Keivan
Jamshidi
drjamshidi2000@gmail.com
1
Department of Veterinary Pathology, Faculty of Veterinary Medicine, IAU Garmsar Branch, Garmsar, Iran
LEAD_AUTHOR
Ozlem
Ozmen
ozlemozmen@mehmetakif.edu.tr
2
Department of Veterinary Pathology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey
AUTHOR
Albayarak, H., Yazici, Z., Okur Gumusova, S. (2007) Seroprevalence to bovine herpesvirus type 1 in sheep in Turkey. Vet Arhiv. 77: 257-263.##
1
Ataseven, V. S., Basaran, Z., Yilmaz, V., Dagalp-Bilge, S. (2010) Seroprevalance of parainfl uenza virus-3 and herpesvirus type-1 infections in goats of Van region. J Fac Vet Med Yuz. Yil 21: 7-9. ##
2
Badieei, K., Ghane, M., Mostaghni, K. (2010) Prevalance of BVD virus antibodies among the industrial dairy cattle herds in suburb of Shiraz, Iran. Middle East J Sci Res. 6: 403-407.##
3
Belak, S., Palfi, V., Palya, V. (1976) Adenovirus infections in lambs, I. Epizootiology of the disease. Zbl. Vet Med B. 23: 320-330.##
4
Belak, S., Vetesi, F., Palfi, V., Papp, L. (1980) Isolation of a pathogenic strain of ovine adenovirus type 5 and a comparison of its pathogenicity with that of another strain of the same serotype. J Comp Pathol. 90: 169-176.##
5
Caswell, J., Williams, K. (2007) Respiratory system. In: Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Maxie, M. (ed.). Edinburgh, UK. Elsevier. p. 594-622.##
6
Ceribaci, A. O., Ozkaraca, M., Ceribaci, S., Ozer, H. (2014) Hist opathologic immunoperoxidase and immunofluorescent examinations on natural cattle pneumonia originated from Parainfluenza type 3, Respiratory Syncytial virus, Adenovirus type 3 and Herpesvirus type 1. Revue Med Vet. 165: 201-212.##
7
Ceribasi, A. O., Ceribasi, S., Ozkaraca, M. (2016) Immunohistochemical detection of bovine herpesvirus type 1 and bovine adenovirus type 3 antigens in frozen and paraffi nized lung sections of pneumonic sheep and goats Vet Arh. 86(1): 9-21.##
8
Cutlip, R. C., Lehmkohl, H. D. Brogden, K. A. Hsu, N. J. (1996) Lesions in lambsexperimentally infected with ovine adenovirus serotype 6 and Pasteurella hemolytica. J Vet Diagn Invest. 8: 296-303.##
9
Cutlip, R. C., Lehmkohl, H. D. (1986) Pulmonary lesions in lambs experimentally infectedwith ovine adenovirus 5 strain RTS-42. Vet Pathol. 23: 589-593.##
10
Cutlip, R. C., Lehmkohl, H. D. (1983) Experimental infection of lambs with ovine adenovirusisolate RTS-15 1: Lesions. Am J Vet Res. 44: 2395-2402.##
11
Debey, B. M., Lehmkuhl, H. D., Chard Bergstrom, C., Hobbs, L. A. (2001) Ovine adenovirus serotype 7-associated mortality in lambs in the United States. Vet Pathol. 38: 644-648.##
12
Elazhary, M. A., Silim, A. Dea, S. (1984) Prevalence of antibodies to bovine respiratorysyncytial virus, bovine viral diarrhea virus, bovine herpesvirus-1, and bovine parainfl uenza-3 virus in sheep and goats in Quebec. Am J Vet Res. 45: 1660- 1662.##
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Ezzi, A., Hatami, A., Bakhshesh, M., Shoukri, M.R., Gharaghozloyan, M. (2013) Serological study of bovine herpesvirus type 1 and parainfluenza type 3 in cow farms of Qazvin province based on different ages and seasons. Arch Razi Inst. 68: 53-57.##
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Giuliani, S., Sharma, R. (1995) Experimental infection of lambs with bovine herpesvirus type-1 (BHV-1). Int J Anim Vet Adv. 10: 73-75.##
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Hajikolaei, M. R. H., Shapouri, M. R. S. A. (2007) Serological study of bovine diarrhea virus infection of cattle in Ahvaz. Vet Res. 62: 21-26.##
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Lehmkohl, H. D., Cutlip, R. C., Bolin, S. R., Brogden, K. A. (1985) Seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. Am J Vet Res. 46: 2601-2604.##
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Lehmkuhl, H. D., Cutlip,R. C., Meehan, J. T., Debey, B. M. (1997) Pathogenesis of infection induced by an adenovirus isolated from a goat. Am J Vet Res. 58: 608-611.##
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Mahmoud, M. A., Ahmed, S. A. (2009) Prevalence of bovine herpes virus-1 in sheep and goats in Egypt. Global Vet. 3: 472-479.##
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Maurice, Y., Provost, A. (1970) Essai d’infection des chevres tchadiennes par le virus de la rhinotracheite infectieuse bovine. Enquete serologique dans l’Ouest Tchadien. Rev Elev Med Vet Pays Trop. 23: 419-423.##
23
Mohantry, S. B., Lillie, M. G., Corselius, N. P., Beck, J. D. (1972) Natu ral infection with lnfectious bovine rhinotracheitis virus in goats. J Am Vet Med Assoc. 160: 879-880.##
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Sakhaee, E. A., Khalili, M., Kazeminia, S. (2009) Serological study of bovine viral respiratory diseases in dairy cattle of Kerman (Iran).Irani J Vet Res. 10: 49-53.##
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Shankar, H., Yadav, M. P. (1987) Experimental infection of sheep with BHV-1 (IBR/IPVvirus) and its possible role in epizootiology. Indian Vet Med J. 11: 71-76.##
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Sharp, J. M., Nettelton, P. F. (2007) Acute respiratory virus infections. In: Diseases of Sheep. Aitken I. D. (ed.). Blackwell Publishing. Edinburgh, Scotland. p. 207-208.##
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Shirvani, E. (2012) Seroepidemiological study of Bovine Respiratory Viruses (BRSV, BoHV-1, PI3, BVDV and BAV-3) in dairy cattle in central region of Iran (Esfahan Province), Trop Anim Health Prod. 44: 191-195.##
31
Six, A., Banks, M., Engels, M., Ros Bascunana, C., Ackerman, M. (2001) Latency and reactivation of bovine herpesvirus 1 (BHV-1) in goats and of caprine herpesvirus 1 (CapHV-1) in calves. Arch Virol. 146: 1325-1335.##
32
Taylor W. P., Okeke, A. N. C., Shidali, N. N. (1977) Prevalence of bovine viral diarrhea andinfectious bovine rhinotracheitis antibodies in Nigerian sheep and goats. Trop Anim Health Prod. 9: 171-175.##
33
Thiry, J., Keuser, V., Muylkens, B., Meurens, F., Gogev, S., Vanderplasschen, A., Thiry, E. (2006) Ruminant alphaherpesviruses related to bovine herpesvirus 1. Vet Res. 37: 169-190.##
34
Trueblood, M. S., Swift, B. L., McHolland Raymond, L. (1978) A bovine Herpesvirusisolated from sheep. Can J Comp Med. 42: 97-99.##
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Yesilbag, K., Dagalp-Bilge, S. (2006) Seroprevalance of bovine herpesvirus 1 infection in sheep in Turkey. Vet J Ankara Univ. 53: 141-143.##
36
Yesilbag, K., Dagalp, S., Okur Gumusova, S., Gungor, B. (2003) Studies on herpesvirus infections of goats in Turkey: prevalance of antibodies to bovine herpesvirus 1. Rev Med. Vet. 154: 772-774.##
37
ORIGINAL_ARTICLE
Growth Performance and the Intestine Morphometry of Japanese Quail That Fed Diets Supplemented With Vitamin E and Selenium
Background: Small intestine and peripheral organs are the main organs for digestion and absorption in the gastrointestinal tract. OBJECTIVES: To investigate effects of different levels of Vitamin E and Selenium on growth performance and small intestine morphometry in Japanese quail. METHODS: A total of 405 mixed sex Japanese quails were randomly allocated into 9 experimental groups by 3×3 factorial design (3 replications and 15 chicken per pen) using Vitamin E (0, 150 and 300 mg/kg) and sodium Selenium (0, 0.2 and 0.4 mg/kg feed) for the entire study. Then, feed intake, body weight gain and food conversion ratio were recorded on days 17-21, 22-35 and 17-35 of the study. At day 35 of the study, 3 birds were randomly selected from each replication, slaughtered and various sections of small intestine (10, 50 and 90% of small intestine length) sampled for morphometry characteristics. Villi height and crypts depth were measured microscopically. RESULTS: According to the results, different levels of Vitamin E and Selenium supplements had no effect on growth performance (P>0.05). Also, supplementation of diet with Vitamin E and Selenium significantly increased villi height and crypts depth in various sections of small intestine on day 35 (P<0.05). CONCLUSIONS: These results suggest Vitamin E and Selenium supplemented diets had beneficial effect on small intestine morphometry characteristics in Japanese quail. Keywords:
https://ijvm.ut.ac.ir/article_68584_292d05c0a61d248873c72ec8e861f92a.pdf
2018-10-01
323
334
10.22059/ijvm.2018.253990.1004886
Intestinal morphometry
Japanese quail
performance
Selenium
vitamin E
Hossein
Zadeh Adamnezhad
adamnezhad@yahoo.com
1
Young Researchers and Elite club, Tabriz Branch, Islamic Azad University, Tabriz, Iran
AUTHOR
Jamshid
GhiasiGhalehkandi
drjamshidghiasi@yahoo.com
2
Department of Veterinary Medicine, Islamic Azad University, Shabestar, Iran
LEAD_AUTHOR
Abdel-Fattah, S.A., El-Daly, E.F., Ali, N.G.M. (2014) Growth performance, immune response, serum metabolites and digestive enzyme activities of Japanese quail fed supplemental L-Carnitine. Global Vet. 12 (2): 277-286.
1
Habibian, M., Ghazi, S., Moeini, M.M., Abdolmohammadi, A. (2014) Effects of dietary selenium and vitamin E on immune response and biological blood parameters of broilers reared under thermoneutral or heat stress conditions. Int J Biometeorol. 58 (5): 741–752.
2
Ayazi, M. (2014) Effect dietary glutamine and vitamin E supplementation on performance, some blood antioxidant indices in broiler chickens under continuous heat stress temperature. Intl J Farm Alli Sci. 3(12): 1303-1310.
3
Chitra, P., Edwin, S.C., Moorthy, M. (2013) Effect of Dietary vitamin E and Selenium Supplementation on Japanese quail Broilers. Ind. J Vet Anim Sci Res. 43(3): 195-205.
4
Clarke, R.M. (1977) The effects of age on mucosal morphology and epithelial cell production in rat small intestine. J Anat. 123: 805-811.
5
Da Rocha, C., Maiorka, A., de Paula Valle, F.L., Gonsales Schramm, V., Angeli, A.L., Fischer da Silva, A.V. (2012) The effect of soybean oil quality and vitamin E supplementation on turkey diet nutrition. J Appl Poult Res. 21:318-324
6
Fan, Y., Croom J., Christensen V., Black B., Bird A., Daniel L., McBride B., Eisen E. (1997) Jejunal glucose uptake and oxygen consumption in turkey poults selected for rapid growth. Poult Sci. 76: 1738-1745.
7
Gajčević, Z., Kralik, G., Has-Schön E., Pavić, V. (2009) Effects of organic selenium supplemented to layer diet on table egg freshness and selenium content. Ital J Anim Sci. 8: 189–199.
8
Ghiasi Ghalehkandi, J., Hassanpour, S., Ebrahimnezhad, Y., Beheshti, R., Maheri-Sis, N. (2014) Intestinal morphograhy of broilers fed diets supplemented with perlite. J Hell Vet Med Soc. 65(2): 99-108.
9
Ghiasi Ghalehkandi, J., Heydarbeygi, J., Ebrahimnezhad, Y., Hassanpour, S. (2015) Effects of pre-incubation laser irradiation on hatchability and small intestine enzymes activity in post-hatched broiler chickens. Bulg J Vet Med. 18(3): 227–238.
10
Ghiasi Ghalehkandi, J., Valil, M.R., Ebrahimnazhad, Y., Salamatdoust Nobar, R., Karamouz, H., Nazeri, M. (2011) Effect of different levels of perlite on performance of broiler chicks. Adv Environ Biol. 5: 776-779.
11
Hussain, M.I., Khan, S.A., Chaudhary, Z.I., Aslam, A., Ashraf, K., Rai, M.F. (2004) Effect of organic and inorganic selenium with and without vitamin E on immune system of broilers. Pak Vet J. 24(1): 1-4.
12
Ibrahim, M.T., Eljack, B.H., Fadlalla, I.M.T. (2011) Selenium supplementation to broiler diets. Anim Sci J. 2(1): 12-17.
13
Khambualai, O., Ruttanavut, J., Kitabatake, M., Goto, H., Erikawa, T., Yamauchi, K. (2009) Effects of dietary natural zeolite including plant extract on growth performance and intestinal histology in Aigamo ducks. Br Poult Sci. 50:123-130.
14
Kucuk, O., Sahin, N., Sahin, K., Gursu, M.F., Gulsu, F., Issi, M. (2003) Egg production, egg quality and lipid peroxidation status in laying hens maintained at a low ambient temperature (60C) and fed a vitamin C and vitamin E supplemented diet. Vet Med. 48: 33-40.
15
Langhout, D.J., Schutte, J.B., Van Leeuwen, P., Wiebenga, J., Tamminga, S. (1999) Effect of dietary high and low methyllated citrus pectin on the activity of the ileal microflora and morphology of the small intestinal wall of broiler chickens. Br Poult Sci. 40: 340-347.
16
Lilburn, M.S., Loeffler, S. (2015) Early intestinal growth and development in poultry. Poult Sci. 94(7): 1569–1576.
17
NRC, (1994) National Research Council. National Requirement of Poultry. 9 Rev. ed., National Academy Press, Washinggton, DC.
18
Olanrewaju, H.A., Thaxton, J.P., Dozier, W.A., Purswell, J., Roush, W.B., Branton, S.L. (2006) A review of lighting programs for broiler production. Intern J Poult Sci. 5: 301–308.
19
Perić, L., Milošević, N., Žikić, D., Kanaćki, Z., Džinić, N., Nollet, L., Spring, P. (2009) Effect of selenium sources on performance and meat characteristics of broiler chickens. J Appl Poult Res. 18: 403–409.
20
Rama Rao, S.V., Prakash, B., Narasimha Raju, M.V.L., Panda, A.K., Poonam, S., Murthy, O.K. (2013) Effect of Supplementing Organic Selenium on Performance, Carcass Traits, Oxidative Parameters and Immune Responses in Commercial Broiler Chickens. Asian -Aust J Anim Sci. 26(2): 247-252.
21
Read-Snyder, J., Edens, F.W., Cantor, A.H., Pescatore, A.J., Pierce, J.L. (2009) Effect of dietary selenium on small intestine villus integrity in reovirus-challenged broilers. Int J Poult Sci. 8 (9): 829-835.
22
Ruttanavut, J., Yamauchi, K., Goto, H., Erikawa, T. (2009) Effects of dietary bamboo charcoal powder including vinegar liquid on growth performance and histological intestinal change in aigamo ducks. Int J Poult Sci. 8: 229-236.
23
Sahin, K., Kucuk, O. (2001) Effects of Vit. E and selenium on performance, digestibility of nutrients and carcass characteristics of Japanese quails reared under heat stress. J Ainm Physiol Anim Nutr. 85(11-12): 342-348.
24
SAS Institute (2001) SAS State Software. SAS Institute, Cary.
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Swain, B.K., Johri, T.S. (2000) Effect of supplementation of different combinations of different levels of selenium and vitamin E on relative weight of some organs and serum enzyme level in broilers. Indian J Poult Sci. 35: 66-69.
26
Swain, B.K., Johri, T.S., Majumdar, S. (2000) Effect of supplementation of vitamin E, selenium and their different combinations on the performance and immune response of broilers. Br Poult Sci. 41: 287-292.
27
Talebali, H., Farzinpour, A. (2006) Effect of different levels of perlite on performance of broiler chicks. Int J Poult Sci. 5: 432-435.
28
Tayeb, I.T., Qader, G.K. (2012) Effect of feed supplementation of selenium and vitamin e on production performance and some hematological parameters of broiler. KSU J Nat Sci. 15(3): 46-56.
29
Wideman, R.F., Rhoads, D.D., Erf, G.F., Anthony, N.B. (2013) Pulmonary arterial hypertension (ascites syndrome) in broilers: A Rev Poult Sci. 92: 64-83.
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Yadav, A., Kadam, S., Pachpande, A.M., Lambate, S.B., Lonkar, V.D., Maini, S., Ravikanth, K. (2010) Post hatch histo-morphological studies of small intestinal development in chicks fed with herbal early chick nutritional supplement. Int J Poult Sci. 9(9): 851-855.
31
Yamauchi, K., Buwjoom, T., Koge, K., Ebashi, T. (2006) Histological intestinal recovery in chickens refed dietary sugar cane extract. Poult Sci. 85: 645-651.
32
ORIGINAL_ARTICLE
Olive Leaves Extract Effects on Sperm Quality Following Experimentally-Induced Diabetes in Rats
Background: The experimental model of streptozotocine (STZ)-induced diabetes explains changes in the male reproductive system as part of the disease. Objective: The aim of the present study was to evaluate the olive leaf extract effects on STZ-induced diabetes and to examine its modulatory effects on sperm quality. Methods: Twenty adult male Sprague–Dawley rats were divided into four equal groups: the first group served as untreated control. Groups 2, 3 and 4 of rats were injected STZ (65 mg/kg). The animals which exhibited blood glucose levels higher than 250 mg/dl by days 4-6, were considered as diabetic rats. Groups 3 and 4 received olive leaf extract (100 and 150 mg/kg, orally) and vehicle to the control and diabetic rats (group 2) for 10 consecutive days. Results: Glycated haemoglobin percentage (%HbA1c) as a diabetic index significantly decreased in the animals ingested by the 150 mg/kg of the extract compared to the diabetic group (P
https://ijvm.ut.ac.ir/article_68470_9e44568f4b59d459fc6bb19c0fd5342c.pdf
2018-10-01
335
346
10.22059/ijvm.2018.253715.1004884
diabetes
Olive extract
rat
Streptozotocine
testis
Masoud
Alirezaei
alirezaei.m54@yahoo.com
1
Division of Biochemistry, School of Veterinary Medicine, Lorestan University, P. O. Box: 465, Khorramabad, Iran. Alirezaei_m54@yahoo.com
LEAD_AUTHOR
Arash
Kheradmand
arashkheradmand@yahoo.com
2
Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, P. O. Box: 465, Khorramabad, Iran
AUTHOR
Pouya
Salahi
pouyasalahi.veterinarian@gmail.com
3
Graduated From the Veterinary Medicine, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
AUTHOR
Afsaneh
Azizi
afazizi733@gmail.com
4
Graduated From the Veterinary Medicine, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
AUTHOR
Agbaje, I.M., Rogers, D.A., McVicar, C.M., McClure, N., Atkinson, A.B., Mallidis, C., Lewis, S.E.M. (2007) Insulin dependent diabetes mellitus: implications for male reproductive function. Hum Reprod. 22(7): 1871-1877.##
1
Al-Azzawie, H.F., Alhamdani, M.S.S. (2006) Hypoglycemic and antioxidant effect of oleuropein in alloxan-diabetic rabbits. Life Sci. 78(12): 1371-1377.##
2
Alirezaei, M., Dezfoulian, O., Kheradmand, A., Neamati, SH., Khonsari A., Pirzadeh, A. (2012a) Hepatoprotective effects of purified oleuropein from olive leaf extract against ethanol-induced damages in the rat. Iran J Vet Res. 13(3): 218-226.##
3
Alirezaei, M., Kheradmand, A., Heydari, R., Tanideh, N., Neamati, S., Rashidipour, M. (2012b) Oleuropein protects against ethanol-induced oxidative stress and modulates sperm quality in the rat testis. Mediterr J Nutr Metab. 5(3): 205-211.##
4
Alves, M.G., Martins, A.D., Rato, L., Moreira, P.I., Socorro, S., Oliveira, P.F. (2013) Molecular mechanisms beyond glucose transport in diabetes-related male infertility. BBA-Mol Bas Dis. 1832(5): 626-635.##
5
American Diabetes A (2016) 2. Classification and diagnosis of diabetes. Diabetes care 39(Supplement 1): S13-S22.##
6
Aydin S. (2010) The presence of the peptides apelin, ghrelin and nesfatin-1 in the human breast milk, and the lowering of their levels in patients with gestational diabetes mellitus. Peptides. 31(12): 2236-2240.##
7
Cancel, A.M., Lobdell, D., Mendola, P., Perreault, S.D. (2000) Objective evaluation of hyperactivated motility in rat spermatozoa using computer-assisted sperm analysis. Hum Reprod. 15(6): 1322-27.##
8
Ceriello, P.A. (2006) Oxidative stress and diabetes-associated complications. Endocr Pract 12(Supplement 1): 60-62.##
9
George, G.E., Uwakwe, A.A. Ibeh, G.O. (2012) Relationship of glycated haemoglobin (HbA1c) and glucose in streptozotocine induced Wistar rats is determined by linear regression. Asian J Med Sci. 3(3): 1-5.##
10
George, G.S., Uwakwe, A.A., Ibeh, G.O. (2013) Glycated haemoglobin, glucose and insulin levels in diabetic treated rats. Can J Pure Appl Sci. 7(1): 2223-2226.##
11
Hamdi, H.K., Castellon, R. (2005) Oleuropein, a non-toxic olive iridoid, is an anti-tumor agent and cytoskeleton disruptor. BBRC. 334(3): 769-778.##
12
Kaiser, Gr.R.R.F., Monteiro, S.C., Gelain, D.P., Souza, L.F., Perry, M.L.S., Bernard, E.A. (2005) Metabolism of amino acids by cultured rat Sertoli cells. Metabolism. 54(4): 515-521.##
13
Kerner, W., Brückel, J. (2014) Definition, classification and diagnosis of diabetes mellitus. Exp Clin Endocrinol Dia. 122(07): 384-386.##
14
Kheradmand, A., Alirezaei, M., Dezfoulian, O. (2013) Cadmium-induced oxidative stress in the rat testes: protective effects of betaine. Int J Pept Res Therap. 19(4): 337-344.##
15
Kheradmand, A., Taati, M., Babaei, H. (2009) The effects of chronic administration of ghrelin on rat sperm quality and membrane integrity. Anim Biol. 59(2): 159-168.##
16
La Vignera, S., Calogero, A.E., Condorelli, R., Lanzafame, F., Giammusso, B., Vicari, E. (2009) Andrological characterization of the patient with diabetes mellitus. Min Endocrinol. 34: 1-9.##
17
Mangoli, E., Talebi, A.R., Anvari, M., Pourentezari, M. (2013) Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice. Int J Reprod Biomed. 11(1): 53-60.##
18
Navarro, Casado, L., Juncos, Tobarra, M.A., Chafer, Rudilla, M., Onzono, L.A.I., Blazquez, Cabrera, J.A., Miralles, Garcia, J.M. (2010) Effect of experimental diabetes and STZ on male fertility capacity. Study in rats. J Androl. 31(6): 584-592.##
19
Sharpe, R.M., McKinnell, C., Kivlin, C., Fisher, J.S. (2003) Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood. Reproduction. 125(6): 769-784.##
20
Sliwa, L., Macura, B. (2005) Evaluation of cell membrane integrity of spermatozoa by hypoosmotic swelling test (water test) in mice after intraperitoneal Daidzein administration. Sys Biol Reprod Med. 51(6): 443-448.##
21
Sِonmez, M., Türk, G., Yüce, A. (2005) The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology. 63(7): 2063-2072.##
22
Sookhthezari, A., Alirezaei, M., Kheradmand, A., Dezfoulian, O. (2015) Streptozotocin-induced Diabetic Effects on the Sperm Fertility Parameters, Glycated Hemoglobin and Total Cholesterol in Mice. SSU J. 23(10): 980-988.##
23
Temidayo, S., du Plessis Stefan, S. (2018) Diabetes mellitus and male infertility. Asian Pac J Reprod. 7(1): 6-14.##
24
Tuck, K.L., Freeman, M.P., Hayball, P.J., Stretch, G.L., Stupans, I. (2001) The in vivo fate of hydroxytyrosol and tyrosol, antioxidant phenolic constituents of olive oil, after intravenous and oral dosing of labeled compounds to rats. J Nutr. 131(7): 1993-1996.##
25
Turner, T.T., Lysiak, J.J. (2008) Oxidative stress: a common factor in testicular dysfunction. J Androl. 29(5): 488-498.##
26
Vignera, S., Condorelli, R., Vicari, E., D’Agata, R., Calogero, AE. (2012) Diabetes mellitus and sperm parameters. J Androl. 33(2): 145-153.##
27
Xiong, W., Wang, H., Wu, H., Chen, Y., Han, D. (2009) Apoptotic spermatogenic cells can be energy sources for Sertoli cells. Reproduction. 137(3): 469-479.##
28
ORIGINAL_ARTICLE
MHC IIB Genetic Diversity and its Association With Humoral Immune Responses in Commercial Turkey
Background: Major histocompatibility complex (MHC) is one of the best characterized genetic regions controlling immune responses against vaccines. Identifying the association between MHC haplotypes and improved immune responses would be useful in genetic breeding strategies in animals. OBJECTIVES: MHC class II B genetic diversity and its association with humoral immune responses against Newcastle vaccine (NDV) were evaluated in commercial turkey poults (meleagris gallopavo). METHODS: A total of 92 turkey poults were vaccinated with live VG/GA strain of Newcastle disease vaccine at age of 10 and 20 days. Serum NDV specific IgY was assessed by indirect enzyme linked immunosorbent assay (ELISA) and MHC II B polymorphism was determined using high resolution melting curve (HRM) technique and DNA sequencing method. Effects of alleles on humoral immune responses were evaluated by multivariate regression analysis and GLM procedures. RESULTS: A total of 8 HRM profiles and 10 alleles were identified in this population. B1-1*3, B1-1*4 and B1-1*5 alleles were significantly associated with lower antibody responses against vaccine in commercial turkey poults. CONCLUSIONS: Three alleles reported in this study were associated with reduced immune responses against NDV vaccine in turkey population. Due to such negative associations, molecular breeding programs based on specific genetic markers should be implemented with great caution.
https://ijvm.ut.ac.ir/article_68514_f90b9a48deaab6ca2ca892346d9c3266.pdf
2018-10-01
347
356
10.22059/ijvm.2018.253776.1004885
Antibody
Immunity
MHC
Turkey
Vaccine
Hassan
Alkaragoly
hassank_ulaiwi@yahoo.com
1
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
AUTHOR
Gholamraza
Nikbakhat Brujeni
nikbakht@ut.ac.ir
2
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
LEAD_AUTHOR
Atefeh
Esmailnejad
esmailnejad82@gmail.com
3
department of pathobiology, school of veterinary medicine, shiraz university, shiraz
AUTHOR
Mohammad
Hasanzadeh
mhzadeh@ut.ac.ir
4
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Tohid
Tolouei
tohid_toloie@yahoo.com
5
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Alexander, D. J., Aldous, E. W., Fuller, C. M. (2012) A selective review of 40 years of Newcastle disease research. Avian Pathol. 41: 329-335.##
1
Al-Karagoly, H., Nikbakht, G., Hassanzadeh, M., Tolouei, T. (2017) Turkey humoral and cell-mediated immune responses to a Newcastle viscerotropic vaccine and its association with Major Histocompatibility Complex. Bulg J Vet Med (BJVM). DOI: 10.15547/bjvm.2031##
2
Briles, W. E., Stone, H. A., Cole, R. K. (1977) Marek’s disease: effects of B histocompatibility alloalleles in resistant and susceptible chicken lines. Science. 195: 193-195.##
3
Butter, C., Staines, K., Van Hateren, A., Davison, T. F., Kaufman, J. (2013) The peptide motif of the single dominantly expressed class I molecule of the chicken MHC can explain the response to a molecular defined vaccine of infectious bursal disease virus (IBDV). Immunogenetics. 65: 609-618.##
4
Calis, J.J., Sanchez‐Perez, G.F., Keşmir, C. (2010) MHC class I molecules exploit the low G+ C content of pathogen genomes for enhanced presentation. Eur J Immunol. 40: 2699-2709.##
5
Chang, C. S., Chen, C. F., Berthouly‐Salazar, C., Chazara, O., Lee, Y. P., Chang, C. M., Chang, K. H., Bed’Hom, B., Tixier‐Boichard, M. (2012) A global analysis of molecular markers and phenotypic traits in local chicken breeds in Taiwan. Anim Genet. 43: 172-182.##
6
Chaves, L.D., Faile, G.M., Kreuth, S.B., Hendrickson, J.A., Reed, K.M. (2010) Haplotype variation, recombination, and gene conversion within the MHC-B of the turkey. Immunogenetics. 62: 465–477.##
7
Chen, F., Pan, L., Chao, W., Dai, Y., Yu, W. (2012) Character of chicken polymorphic major histocompatibility complex class II alleles of 3 Chinese local breeds. Poult Sci. 91: 1097-1104.##
8
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Erhard, M., Von Quistorp, I., Schranner, I., Jüngling, A., Kaspers, B., Schmidt, P., Kühlmann, R. (1992) Development of specific enzyme-linked immunosorbent antibody assay systems for the detection of chicken immunoglobulins G, M, and A using monoclonal antibodies. Poult Sci. 71: 302-310.##
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Nikbakht G, Esmailnejad A. (2015) Chicken major histocompatibility complex polymorphism and its association with production traits. Immunogenetics. 67(4):247-252.##
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28
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29
ORIGINAL_ARTICLE
The Effects of L-Citrulline Pretreatment on the Isometric Tension of the Isolated Perfused Rat Aorta
Background: The achievement of an effective compound for prevention/treatment of hypertension with fewer complications has been of interest in recent years. OBJECTIVES: This study aimed to examine the effects of L-citrulline pretreatment on the tension of isolated rat aortic tissues precontracted by different vasoconstrictors. METHODS: Sixteen male Wistar rats (300-350g) were randomly divided into two groups of control and test. The control group was injected 1ml distilled water, while the animal in the test group received 200 mg/Kg L-citrulline (CIT) i.p. for 7days. Rats were euthanized, their thoracic aortas were immediately separated and placed into a petridish containing cold Kerebs-Henseleit solution (KHS). The aorta were cleaned of the surrounding tissues and cut into 4 rings in the presence of 95% O2+5% CO2. The aortic rings divided into 6 subgroups, were suspended into organ bath containing KHS at 37oC. The isolated rings were contracted by 2×10-6 M phenylephrine (Phe) and 60Mm KCl. When the plateau was reached, a cumulative concentration of acetylcholine (Ach) was added into organ bath to induce relaxation. The effects of CIT on relaxation and the role of NO were tested using L-NAME as a pharmacological probe. RESULTS: The pretreatment of rats by CIT significantly (P<0.05) reduced the plateau contraction induced by Phe. CIT also significantly (P<0.01) decreased the contraction induced by KCl and L-NAME+Phe. However, cumulative addition of ACh significantly (P<0.001) decreased the vasoconstriction induced by Phe but not by KCl and L-NAME+Phe in both control and CIT-treated group. CONCLUSIONS: It suggests that, CIT can reduce the rat aorta vasoconstriction through releasing NO.
https://ijvm.ut.ac.ir/article_68661_870c8539eeeb2d0c7ca18eccad3d3d1d.pdf
2018-10-01
357
367
10.22059/ijvm.2018.267039.1004930
Aortic ring
Hypertension
L-citrulline
NO
Vasorelaxation
Seyyed Reza
Hashemi
rhashemi1@gmail.com
1
Department of Comparative Biosciences, Faculty of Veterinary Medicine
AUTHOR
Hossein Ali
Arab
harab@ut.ac.ir
2
Department of Comparative Biological Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
Behjat
Seifi
b-seifi@tums.ac.ir
3
Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Samad
Muhammadnejad
smuhammadnejad@gmail.com
4
Cell-Based Therapies Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Allerton, T., Proctor, D., Stephens, J., Dugas, T., Spielmann, G., Irving, B. (2018) L-Citrulline Supplementation: Impact on Cardiometabolic Health. Nutrients. 10(7): 1-24. doi: 10.3390/nu10070921. PMID: 30029482.
1
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36
ORIGINAL_ARTICLE
Effect of Conjugated Linoleic Acid Supplement and Sesame as Source of N-6 on Performance and Milk Fatty Acid Profile of Holstein Dairy Cows During Transition Period
Background: Feeding unsaturated fatty acids sources in dairy cows is considered as a strategy to improve animal health during postpartum period and milk fatty acid profile. OBJECTIVES: This study aimed to investigate the effects of conjugated linoleic acid supplement and combination of sesame meal and oil on performance and milk fatty acid composition of dairy cows during transition period. METHODS: Twenty-one multiparous Holstein cows (249 days of pregnancy) were randomly assigned to 3 dietary treatments including: 1) control with a source of calcium salts of palm oil (0.95 and 1.68% of the dry matter in prepartum and postpartum diets, respectively), 2) conjugated linoleic acid supplementation (0.4 and 0.5% of the dry matter in prepartum and postpartum diets, respectively) 3) sesame meal (2.18 and 4.32% of the dry matter in prepartum and postpartum diets, respectively) and calcium salt of sesame oil (0.83 and 1.4% of the dry matter in prepartum and postpartum diets, respectively) as source of n-6. RESULTS: Dry matter intake, body weight, body condition score, average milk yield and component including fat, protein and lactose were not affected by dietary treatments. The proportion of short and medium chain fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were not affected by dietary treatments. Conjugated linoleic acid supplement numerically increased content of cis-9, trans-11 conjugated linoleic acid and reduced short chain fatty acids ( CONCLUSIONS: Feeding conjugated linoleic acid supplement and combination of sesame meal and oil had no effect on performance, milk composition and fatty acid profile.
https://ijvm.ut.ac.ir/article_68519_539422eb6c4e82d0dfa722b6c48d8a96.pdf
2018-10-01
369
379
10.22059/ijvm.2018.248538.1004867
CLA
Sesame
Transition period
Neda
Sheikh
n.sheikh54@ut.ac.ir
1
Department of Animal Sciences, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Abolfazl
Zali
zalia@ut.ac.ir
2
Department of Animal Sciences, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Mahdi
Ganjkhanlou
ganjkhanlou@ut.ac.ir
3
Department of Animal Sciences, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
LEAD_AUTHOR
Armin
Towhidi
atowhidi@ut.ac.ir
4
Department of Animal Sciences, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Mehdi
Dehghan Banadaki
dehghanb@ut.ac.ir
5
Department of Animal Sciences, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
AbuGhazaleh, A. A., Schingoethe, D. J., Hippen, A. R., Kalscheur, K. F., Whitlock, L. A. (2002) Fatty acid profiles of milk and rumen digesta from cows fed fish oil, extruded soybeans or their blend. J Dairy Sci. 85: 2266–2276.##
1
AlZahal, O., Odongo, N. E., Mutsvangwa, T., Or-Rashid, M. M., Duffield, T. F., Bagg, R., Dick, P., Vessie, G., McBride, B.W. (2008) Effects of monensin and dietary soybean oil on milk fat percentage and milk fatty acid profile in lactating dairy cows. J Dairy Sci. 91: 1166–1174.##
2
Bauman, D. E., Corl, B. A., Baumgard, L. H., Griinari, J. M. (2001) Conjugated linoleic acid (CLA) and the dairy cow. In: Recent Advancesin Animal Nutrition. Garnsworthy, P. C., Wiseman, J. (eds.). Nottingham University Press, Nottingham, UK. p. 221-250.##
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Bauman, D.E., Griinari, J.M. (2001) Regulation and nutritional manipulation of milk fat: low-fat milk syndrome. Livest Prod Sci. 70: 15–29.##
4
Bernal-Santos, G., Perfield, J. W., Barbano, D. M., Bauman, D. E., Overton, T. R. (2003) Production responses of dairy cows to dietary supplementation with conjugated linoleic acid (CLA) during the transition period and early lactation. J Dairy Sci. 86: 3218–3228.##
5
Chandler, T. L., Fugate, R. T., Jendza, J. A., Troescher, A., White, H. M. (2017) Conjugated linoleic acid supplementation during the transition period increased milk production in primiparous and multiparous dairy cows. Anim Feed Sci Tech. 224: 90–103.##
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Chen, P., Ji, P., Cao, Z.J., Li, S.L. (2007) Effect of processing whole cottonseed on yield and composition of milk in dairy cows. J Anim Feed Sci. 16 (2): 531–536.##
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Gardinal, R.G., Calomeni, D., Zanferari, F., Vendramini, T. H. A., Takiya, C. S., Del Valle, T. A., Renno, F. P. (2016) Different durations of whole raw soybean supplementation during the prepartum period: Milk fatty acid profile and oocyte and embryo quality of early-lactating Holstein cows. J Dairy Sci. 101: 1–15.##
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17
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ORIGINAL_ARTICLE
First Report of Ixodes ricinus and Rhipicephalus turanicus Infestation in Persian Leopard (Panthera Pardus Saxicolor), Golestan National Park, Iran
A carcass of a Persian leopard (Panthera pardus saxicolor) that was inadvertently killed in a car accident in Golestan National Park, North-Iran, was referred to the laboratory of the Department of Environment in Golestan Province. The carcass was infested with hard ticks. Five ticks were collected and identified as Ixodes ricinus (one female) and Rhipicephalus turanicus (four males). These hard ticks are major vectors of some important microorganisms of veterinary and medical significance. This is the first detection of I. ricinus and R. turanicus in the Persian leopard.
https://ijvm.ut.ac.ir/article_68502_4e283453ad969c1de460420fff050cb4.pdf
2018-10-01
381
385
10.22059/ijvm.2018.256802.1004896
Golestan Park
Ixodes ricinus
Persian leopard
Rhipicephalus turanicus
Somayeh
Namroodi
snamroodi2000@yahoo.com
1
Department of Environmental Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran
LEAD_AUTHOR
Fatemeh
Arabkhazaeli
farab@ut.ac.ir
2
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
James Norman
Miils
jnormanmills@gmail.com
3
Emory University, Atlanta, Georgia, United States of America
AUTHOR
. Esteve-Gassent, M.D., Pérez de León, A.A., Romero-Salas, D., Feria-Arroyo, T.P., Patino, R., Castro-Arellano, I., Gordillo-Pérez, G., Auclair, A., Goolsby, J., Rodriguez-Vivas, R.I., Estrada-Franco, J.G. (2014) Pathogenic landscape of transboundary zoonotic diseases in the Mexico–US border along the Rio Grande. Frontiers in Public Health. 2: 177.##
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