University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Isolation of bovine spermatogonial cells and co-culture with prepubertal sertoli cells in the presence of colony stimulating factor-1
83
90
EN
Shiva
Shafiei
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
shiva_shafiei@yahoo.com
Parviz
Tajik
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
ptajik@ut.ac.ir
Hamid
Ghasemzadeh-nava
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
hghasem@ut.ac.ir
Mansoureh
Movahedin
Department of Anatomy, Faculty of Medical Sciences, University of Tarbiat Modares, Tehran, Iran
mansoure@modares.ac.ir
Massoud
Talebkhan Garoussi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
garoussi@um.ac.ir
Babak
Qasemi-Panahi
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
babakpanahi98@yahoo.com
Peyman
Rahimi Feyli
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
p.rahimi64@yahoo.com
10.22059/ijvm.2013.35058
BACKGROUND: Spermatogonial stem cells (SSCs) are infrequent<br />self-renewing cells among the type A spermatogonia<br />within the seminiferous tubules and are the basis of spermatogenesis<br />in mammalian testis. An adequate number of SSCs is a<br />primary requirement for the study of their behavior, regulation, and<br />further biomanipulation. OBJECTIVES: In this paper, we studied<br />the development of the primary co-cultures of type A spermatogonia<br />and prepubertal bovine sertoli cells in the presence of Colony<br />Stimulating Factor 1 (CSF1), a potential contributor in the SSC<br />niche. METHODS: The effect of different concentrations of CSF1<br />(0, 10, 50 and 100 ng/mL) on the colonization activity of spermatogonial<br />cells was assessed 4, 7 and 11 days after the beginning of the<br />culture by counting the total number of colonies and measuring their<br />area in each group of the present experiment. Immunofluorescent<br />staining against OCT4 and vimentin led to the confirmation of the<br />nature of both the SSCs and sertoli cells. RESULTS: Results showed<br />that the total number of colonies from day 4 to 11 increased<br />significantly in all groups, independent of CSF1 concentration. In<br />addition, the total number and total area of colonies were higher (not<br />significant) in 10 and 50 ng/mL CSF1 treatments than the control<br />and 100 ng/mL CSF1 groups in all the three evaluations during the<br />experiment. However, this difference was only significant (p<0.05)<br />between the total area of colonies in the control and 10 ng/mLCSF1<br />groups at day 4 of co-culture. CONCLUSIONS:It was concluded that<br />CSF1 can be a suitable growth factor for improving SSCs colonization<br />in vitro, particularly during the first days of culture where<br />accompanying sertoli cells still have not proliferated sufficiently to<br />support the propagating spermatogonial cells.
spermatogonial stem cell,CSF1,bovine spermatogonia,SSC coculture,SSC niche
https://ijvm.ut.ac.ir/article_35058.html
https://ijvm.ut.ac.ir/article_35058_50993406e8621959632d0110e3b4c494.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Normal values and seasonal differences in the serum concentration of vitamin Aand beta-carotene in the Iranian camel (Camelus dromedarius)
91
94
EN
Alireza
Ghadrdan Mashhadi
Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
kianeg2000@yahoo.com
Alireza
Sazmand
Department of Agriculture, Payam e Noor University, Tehran, Iran
alireza_sazmand@yahoo.com
Abbas
Karimiyan
Graduated from the School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
abbaskarimian@yahoo.com
Seyed Hossein
Hekmati Moghaddam
School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
shhekamti2002@yahoo.com
10.22059/ijvm.2013.35056
BACKGROUND: Vitamin A which is found in different tissues<br />and organs plays a particular role in detecting clinical signs in<br />various deficiency conditions. However, sometimes the marginal<br />deficiency is present in a way that clinical signs are not visible but<br />performance defects, such as infertility is seen. OBJECTIVES: In<br />this study, the normal baseline levels of vitamin Aand β-carotene in<br />clinically healthy camels (Camelus dromedarius) in the Yazd<br />province were investigated. METHODS: A total of 168 Iranian<br />camels of both sexes were sampled from February 2009 to July<br />2010. Spectrophoto-metry was used for measuring the serum<br />values. RESULTS: The mean±SE concentration of vitamin Aand β-<br />carotene were 63.9±4.7 and 9±1.1 μg/dL, respectively. Although,<br />the β-carotene concentr-ation was significantly higher in summer,<br />vitamin Awas not influenced by season. No significant difference<br />in the serum levels of the measured parameters was observed in<br />different ages and sexes. CONCLUSIONS: The results of this study,<br />for the first time, indicate the concentration of vitamin A and β-<br />carotene in the camels in Iran. This finding can be used as a reference<br />guide for evaluation of the deficiency or excess of vitamin Aand β-<br />carotene in camels in Iran. Furthermore, due to the lower levels of<br />vitamin A and β-carotene in Iranian dromedaries during winter,<br />supplementary feeding of vitamin A is recommended during this<br />season.
vitamin A,beta-carotene,Camel,Season
https://ijvm.ut.ac.ir/article_35056.html
https://ijvm.ut.ac.ir/article_35056_dfe8e787954a954e7c8e3fc98f2a45a0.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Relative effectiveness of herbal methionine compared to DLmethionine on growth, performance and carcass responses basis in broiler chickens
95
101
EN
Sheila
Hadinia
Department of Animal Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
sheila_hadinia@ut.ac.ir
Mahmood
Shivazad
Department of Animal Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
shivazad@ut.ac.ir
Hossein
Moravej
Associate Professor of poultry nutritionDepartment of Animal Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
hmoraveg@ut.ac.ir
Majid
Alahyari-Shahrasb
Department of Animal Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
majid.alahyari@gmail.com
10.22059/ijvm.2013.35062
BACKGROUND: The degree to which the amount of an<br />ingested nutrient is absorbed and available to the body is called<br />bioavailability. OBJECTIVES: Relative effectiveness of herbal<br />methionine (H-Met®) compared to DL-methionine (DL-Met)<br />was investigated in this experiment. METHODS: Exponential<br />regression analysis was used to determine bioefficacy of H-Met®<br />based on body weight gain, feed intake and feed conversion. DLMet<br />and H-Met® were added to a basal diet in 3 and 4 levels,<br />respectively, in starter, grower and finisher periods. Therefore,<br />that met the nutrient and energy requirements of broiler chickens,<br />with the exception of Met+Cys. RESULTS: In the 42-d trial,<br />broilers growth increased significantly (p<0.05), relative to<br />those broilers fed basal diet, regardless of Met sources. Carcass<br />characteristics did not respond significantly to the supplemental<br />Met. CONCLUSIONS: Regression analysis revealed that H-Met®<br />was 52% (body weight gain), 72% (feed intake) and 77% (feed<br />conversion ratio) as efficacious as DL-Met. H-Met® can be<br />administered as a new and a natural source of Met in poultry<br />industry.
herbal methionine,broiler,Carcass,bioefficacy
https://ijvm.ut.ac.ir/article_35062.html
https://ijvm.ut.ac.ir/article_35062_3a2b45bfaf8aa56311ec42d106344d73.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
The study of the plerocercoid of diphyllobothriidae (cestoda, pseudophyllidea) in two cyprinid hosts, Abramis brama and Alburnoides bipunctatus from north and northwest of Iran
103
109
EN
Emad
Ahmadi Ara
University of Tehran,Faculty of Veterinary MedicineDepartment of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
ahmadiara@ut.ac.ir
Seid Hosein
Hoseini
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
hhoseini@ut.ac.ir
Fatemeh
Jalosian
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
fjalusian@ut.ac.ir
Hossain Ali
Ebrahimzadeh Husavi
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
ebrahimzadeh@ut.ac.ir
Sina
Sachaeifar
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
sinasahaeifa@gmail.com
Abbas
Gerami Sadeghiyan
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
gerami@gmail.com
10.22059/ijvm.2013.35060
BACKGROUND: The plerocercoid stage of Pseudophyllidean<br />cestoda infected a wide range of fresh water fish,<br />particularly the members of the Cyprinidae family. The parasite<br />species are the most common pathogens that have severe effects<br />on fish. OBJECTIVES: The aim of the present study is to<br />determine the occurrence and distribution of the plerocercoid of<br />Diphyllobothriidae in two freshwater fish from north and<br />northwest of Iran. Finally, we discuss the role and dynamics of<br />these species of fish in the transmission of infection. METHODS:<br />This study was carried out from September 2011 to September<br />2012on a total of 883 A. bipunctatus and 418 A. brama from north<br />and northwest of Iran. The samples were analyzed to find the<br />plerocercoid infection. RESULTS: From a total number of 883 A.<br />bipunctatus and 418 A. brama fish samples, 558 fish (63.19%)<br />of the former and 67 fish (16.02%) of the latter were infected. The<br />rate of infection was significantly lower in winter (p<0.01). Also,<br />the weight of infected fish was significantly lower than noninfected<br />ones (p<0.01). Moreover, the infection in northwest of<br />Iran was significantly higher than north of Iran (p<0.01).<br />CONCLUSIONS: The family of Diphyllobothriidae is an important<br />cestode and the prevention programs to break the cycle<br />of infection are essential. More suitable solutions to tackle the<br />problem, further epidemiological studies on other fresh water<br />sources of Iran are needed.
diphyllobothriidae,Abramis brama,Alburnoides bipunctatus,north and
northwest of Iran
https://ijvm.ut.ac.ir/article_35060.html
https://ijvm.ut.ac.ir/article_35060_2c09e1c27aeab9e4cee62fdc9f8e91e5.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Acomparative osteometric evaluation of some cranial indices of clinical significance in goats (Capra hircus) from the middlebelt regions of Nigeria
111
116
EN
O.M.
Samuel
Department of Veterinary Anatomy, University of Agriculture Makurdi, Benue state, Nigeria; Department of Veterinary Anatomy, University of Ibadan, Ibadan, Oyo state, Nigeria
walesamuel10@gmail.com
B.O.
Korzerzer
Department of Veterinary Anatomy, University of Agriculture Makurdi, Benue state, Nigeria
wesamuel10@gmail.com
J. O.
Olopade
Department of Veterinary Anatomy, University of Ibadan, Ibadan, Oyo state, Nigeria
waleamuel10@gmail.com
S. K.
Onwuka
Department of Veterinary Anatomy, University of Ibadan, Ibadan, Oyo state, Nigeria
walesmuel10@gmail.com
10.22059/ijvm.2013.35315
BACKGROUND:There is no comprehension data on anatomical<br />indices of Nigerian goats. OBJECTIVES: To show osteometric<br />values of some cranial indices in Nigerian goats. METHODS:<br />Sixty (60) goat skulls that were found around Makurdi, Kwande,<br />Katsina-ala and the neighbouring Adamawa state of Nigeria,<br />were studied by investigating the 30 craniometric values.<br />RESULTS: The measured distance from the facial tuberosity to<br />the infraorbital foramen was 2.33±0.29 Cm and 1.80±0.14 Cm<br />for females and males, respectively. In addition, the distance<br />from the medial canthus to the supraorbital foramen was 27±0.31<br />Cm for females and 2.83±0.24 Cm for males; from the lateral<br />alveolar root to the mental foramen was 2.05±0.07 Cm for<br />females and 5.10±7.28 Cm for males; and from the mandibular<br />foramen to the ventral border of mandible was 5.90±0.14 Cm for<br />females and 5.48±0.43 Cm for males. CONCLUSIONS: This<br />breed of goat might be a different phenotype of the breeds that is<br />available in Nigeria.
skullmorphometry,goat breeds,craniofacialindices,Phenotype
https://ijvm.ut.ac.ir/article_35315.html
https://ijvm.ut.ac.ir/article_35315_8b5180418b75ff8371275c131d78e2a6.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
The submandibular salivary gland microscopic morphology of the adult African giant pouched rat (Cricetomys gambianus, waterhouse-1840)
117
122
EN
E.
Ikpegbu
Department of Veterinary Anatomy, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria
U.C.
Nlebedum
Department of Veterinary Anatomy, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria
Introduction
The major mammalian salivary glands include the
mandibular, submandibular,
O.
Nnadozie
Department of Veterinary Anatomy, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria
I.O.
Agbakwuru
Department of Veterinary Anatomy, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria
10.22059/ijvm.2013.35491
BACKGROUND: In the present study, the submandibular<br />salivary gland microscopic morphology of the adult African<br />giant pouched rat was investigated. This study was carried out to<br />provide the basic histology of this organ in the giant pouched rat,<br />to accompany the dearth of information of its microscopic<br />architecture in the available literature. This becomes of even<br />higher importance when considering the possible use of this<br />species of rodent as a future laboratory animal to replace the<br />Winster rat, because of its bigger size and the possibility of<br />domesticating the giant pouched rat as a ready source of animal<br />protein. In addition, the need to understand the digestive biology<br />to help animal nutritionists in feeding formulation may also be<br />achieved. The histology revealed the presence of both serous and<br />mucus secretory acini. Some mucus cells showed serous<br />demilumes. The myoeithelial cells were seen around the<br />secretory cells and the intercalated ducts. The serous gland<br />region with more relatively profuse intralobular ducts was larger<br />in size than the mucus gland region. The intralobular ducts of<br />intercalated and striated ducts were lined by simple cuboidal and<br />simple columnar cells, respectively. The excretory duct was<br />lined by the stratified cuboidal cells. The large serous glandular<br />region reflects need for more enzymic action in the oral cavity,<br />while the mucus glands will help produce mucin that will<br />lubricate the digestive tract. This study, for the first time<br />documents the normal histology of submandibular salivary<br />gland in this species, hence filling the knowledge gap that will<br />help further research especially on the role of myoepihelial cells<br />in the secretory glands tumours.
Submandibular gland,Histology,myoepithelial cells,african giant
pouched rat
https://ijvm.ut.ac.ir/article_35491.html
https://ijvm.ut.ac.ir/article_35491_08fbb1595f5dfbcd0b55eed4b6a75cf8.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Use of N-trimethyl chitosan for intranasal delivery of DNA encoding M2e-HSP70c in mice
123
128
EN
Mehran
Dabaghian
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
mdabaghian@ut.ac.ir
Seyyed Mahmoud
Ebrahimi
Research Center of Virus and Vaccine, Baqiyatallah University of Medical Sciences, Tehran, Iran
smebrahimi@shirazu.ac.ir
Gholamraza
Nikbakhat Borojeni
0000-0002-2970-5827
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
nikbakht@ut.ac.ir
Majid
Tebianian
Department of Biotechnology, Razi Vaccine and Serum Research Institute (RVSRI), Karaj, Iran
tebyanian@yahoo.com
Ali
Rezaei Mokaram
Department of Biotechnology, Razi Vaccine and Serum Research Institute (RVSRI), Karaj, Iran
rezaemokaram@yahoo.com
Maryam
Iman
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
maryamiman@yahoo.com
Alireza
Tavangar Ranjbar
Department of Virology, Tehran University of Medical Sciences, Tehran, Iran
tavangarranjbar@yahoo.com
10.22059/ijvm.2013.35063
BACKGROUND: Influenza outbreak has become a great lifethreatening<br />disease in the world. Nasal vaccines can induce<br />systemic IgG and mucosal IgA antibody responses, which<br />establish two layers of immune defense against the infectious<br />pathogens like influenza. Mucosal vaccines must overcome<br />several limitations, including the mucociliary clearance and<br />inefficient uptake of soluble antigens. Therefore, nasal vaccines<br />require potent adjuvants and delivery systems. OBJECTIVES: In<br />this study we evaluated the effect of N-trimethyl chitosan (TMC)<br />as a potent vehicle for DNA encoding M2e/HSP70c in order for<br />intranasal administration in mice. METHODS:Ectodomain of the<br />conserved influenza matrix protein 2 (M2e), which has been<br />found to induce heterosubtypic immunity, was fused to<br />HSP70359-610 or C-terminus of Mycobacterium tuberculosis<br />HSP70 (HSP70c) in pcDNA3.1 vector (pcDNA/M2e-HSP70c)<br />and then encapsulated into a derivative of chitosan, N-trimethyl<br />chitosan (TMC). After encapsulation of the plasmid, physical<br />properties of the particles were investigated using Zetasizer®<br />3000 the particles were then administered through the intranasal<br />delivery in BALB/c mice. RESULTS: It was found that the<br />particles had a size ranging between 90-120nm and positive<br />surface charge. The intranasal immunization with M2e-<br />HSP70c+TMC in BALB/c mice significantly induced higher<br />M2e specific IgG than those induced in control groups<br />(pcDNA/M2e-HSP70c without TMC, pcDNA/M2e, bearing<br />M2e alone, and PBS).CONCLUSIONS: The present study showed<br />that the encapsulation of M2e/ HSP70c into N-trimethyl<br />chitosan (TMC) could strongly induce the humoral immune<br />response against the M2e-HSP70c plasmid without lowering the<br />adjuvant efficacy of HSP70c.
influenza,M2e,HSP70,N-trimethyl
chitosan (TMC),Intra nasal delivery
https://ijvm.ut.ac.ir/article_35063.html
https://ijvm.ut.ac.ir/article_35063_090ff952d3729a2910a1a45bec562494.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Preventive effect of omega-3 fatty acids on ethinyl estradiolinduced hepatosteatosis in female wistar rat
129
134
EN
Marjan
Chahardahcherik
Department of Basic Sciences, Faculty of Veterinary Medicine Shahid Chamran University, Ahwaz, Iran
adamtane_2005@yahoo.com
Ali
Shahriari
Department of Basic Sciences, Faculty of Veterinary Medicine Shahid Chamran University, Ahwaz, Iran
a.shahriari@scu.ac.ir
Peyman
Asadian
Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran
asadianp@gmail.com
Saleh
Esmaeilzadeh
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahwaz. Iran.
s_esmaeilzadeh@scu.ac.ir
10.22059/ijvm.2013.35061
BACKGROUND: Hepatic steatosis due to estrogen therapy<br />increases the activity of inflammatory markers, particularly the<br />activity of TNFα which in turn induces more lipogenesis.<br />Omega-3 fatty acids are among the negative regulators of hepatic<br />lipogenesis. OBJECTIVES: In this research, the preventive effect<br />of omega-3 fatty acids on estrogen-induced steatosis in rats was<br />evaluated. METHODS:2 mg/kg. BW/SC of 17α-ethiny-lestrasdiol<br />were injected into 25 female wistar rats in 5 equal groups<br />(excluding the control group) over 10 consecutive days.<br />Simultaneously, 3 of estradiol-treated groups were orally given<br />250, 500, and 1000 mg/kg. BW omega-3 fatty acids, respectively.<br />At the end of the experiment, plasma ALT, AST, and TNFα<br />level were determined. Histopathological changes in the liver<br />were also identified by the evaluation of samples stained with<br />H&E and Oil Red O. RESULTS: The histological findings<br />revealed hepatic microvesicular steatosis and fat deposit in<br />ethinylestradiol and, to a lesser extent, in the 250 mg/kg BW<br />omega-3 fatty acids groups. The plasma levels of AST, ALT, and<br />TNFα significantly increased in the ethinylestradiol group<br />compared to the control (p<0.05) and 1000 mg/kg. B.W omega-<br />3 group. Omega-3 fatty acids reduced these parameters in<br />comparison to the estradiol group (p<0.05). CONCLUSIONS: It<br />was concluded that 1000mg/kg.BW of omega-3 protects the<br />liver against steatotic injuries.
hepatosteatosis,rat,ethinylestradiol,AST,ALT
https://ijvm.ut.ac.ir/article_35061.html
https://ijvm.ut.ac.ir/article_35061_ffb3d5ed7a56a8a9e43ae22e7944fc5b.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Detection of avian reoviruses causing tenosynovitis in breeder flocks in Iran by reverse transcription-polymerase chain reaction (RT-PCR) and restriction enzyme fragment length polymorphism (RFLP)
135
142
EN
Mahdi
Hedayati
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
mahvet70@yahoo.com
Bahram
Shojadost
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
bshojae@ut.ac.ir
Seyed Mostafa
Peighambari
0000-0001-9166-1303
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
mpeigham@ut.ac.ir
10.22059/ijvm.2013.35057
BACKGROUND:Avian reoviruses (ARVs) are members of the<br />Orthoreovirus genus; one of the 12 genera of the Reoviridae<br />family. The ARVs are the cause of some important diseases in<br />poultry such as reovirus-induced arthritis, tenosynovitis,<br />chronic respiratory disease, and mal-absorption syndrome.<br />OBJECTIVES: In this study, the presence of ARVs in the Iranian<br />breeder flocks was investigated through reverse transcriptionpolymerase<br />chain reaction (RT-PCR) and restriction enzyme<br />fragment length polymorphism (RFLP). METHODS: A total of<br />800 fecal swab samples were initially collected from breeder<br />flocks (older than 45 weeks of age). They were then sent to the<br />laboratory in containers with PBS, and after that they were<br />pooled and finally to 120 samples were obtained. The total RNA<br />extracted from the pooled fecal samples were used to amplify the<br />selected parts of the S1 (1023 bp) and S4 (437 bp) genes from the<br />ARV field isolates using RT-PCR. The positive RT-PCR<br />amplified products were further analyzed by RFLP using five<br />restriction enzymes. RESULTS: Based on the findings, 5 samples<br />were positive with the S1 primer and 6 samples were with the S4<br />one. The patterns observed after the digestion of PCR products<br />revealed that the isolates of this study were identical to both the<br />S1133 vaccine and standard strains. CONCLUSIONS: The<br />findings suggested that the RT-PCR/RFLP analysis might be<br />considered as a simple and rapid approach for the differentiation<br />of ARVisolates. This study was the first molecular detection of<br />the ARVs presence in the Iranian breeder flocks using the RTPCR<br />amplification of the S1 and S4 genes and RFLP analysis.
Avian reovirus,breeder flocks,RTPCR,RFLP
https://ijvm.ut.ac.ir/article_35057.html
https://ijvm.ut.ac.ir/article_35057_9089568420614a44d367d2b5259bfaab.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
7
2
2013
07
01
Footpad horn hypertrophy and vertical fissure accompanied by white line disease 2 in case of concurrent deep digital sepsis in a culling cow
143
147
EN
Mohsen
Nouri
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department
mnouri2@yahoo.com
Seyed Hossein
Mardjanmehr
0009-0000-6779-3357
Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
hmehr@ut.ac.ir
Alireza
Vajhi
0000-0002-8048-7124
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
avajhi@ut.ac.ir
Iraj
Norozian
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
norozian47@yahoo.com
Davoud
Faskhoudi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
davoodfaskhoodi@gmail.com
10.22059/ijvm.2013.35059
A5.2 year-old Holstein cow was presented with lameness and<br />abnormal weight bearing in a slaughterhouse. Widening of the<br />periople and swelling of the heel area in the lateral claw was more<br />permanent than the medial claw. Vertical fissure (VF) involving<br />the coronary band to the middle of the dorsal wall of the medial<br />hind claw was observed. It was also extended to the bearing<br />surface in the inner surface of the claw wall. White line disease 2<br />(WLD2) was seen in the length of VF on the bearing surface. The<br />horn of the bulb apex of the lateral hind claw was hypertrophic.<br />There was a groove in the footpad between the apex and base of<br />the bulb too. Grosspathologically, the digital bones showed a<br />wide range of bone changes such as new bone formation, pedal<br />osteolysis and bony ankylosis. The hind leg was amputated at the<br />tarsus immediately after slaughter. The lower limbs of lame cows<br />were examined and the type and distribution of digital lesions<br />were tabulated. An x-ray machine was used in this study to take<br />radiographic images. The radiographic images taken from the<br />involved digits of the affected case depicted a quite wide range<br />of radiographic signs. After the first review of the X-ray film,<br />samples were selected for further procedures through<br />putrefaction. Laminitis may have affected this case at some<br />previous time. Routine trimming seems to have prevented the<br />premature culling in the cows with claw lesion.
Lameness,Deep Sepsis,Vertical
Fissure,White Line Disease 2,hypertrophy
https://ijvm.ut.ac.ir/article_35059.html
https://ijvm.ut.ac.ir/article_35059_32fa7b31409d6891a5d72d1dda5436bf.pdf