University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
An investigation on the possibility of using eCG for presynchronization prior to ovsynch in dairy cow
85
97
EN
Amir
Niasari-Naslaji
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran
niasari@ut.ac.ir
Hadi
Kavousi Nodar
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran
hadi.kavousi@ut.ac.ir
Mahdi
Vojgani
Department of Theriogelogy, Faculty of Veterinary Medicine, University of Tehran
vodjgani@ut.ac.ir
Farid
Heidari
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
heidarif@gmail.com
10.22059/ijvm.2018.247457.1004864
<strong>Background:</strong> Presynchronization could enhance fertility in timed breeding programs. The best presynchronization method has to justify biological requirements and to provide practical and economic advantages. <strong>OBJECTIVES: </strong>Present study investigated the effectiveness of presynchronization with equine chorionic gonadotropin (eCG) before ovsynch (eCG-ovsynch) compared to conventional presynch-ovsynch program (2PG-ovsynch) in lactating cows. <strong>METHODS:</strong> Healthy lactating cyclic Holstein cows (n=110) without any particular pre, peri and post parturient problems were selected for this study. The first insemination following timed breeding program was conducted at 57 days after calving. Ovsynch (timed breeding program) consisted of two GnRH analogue injections, at 7 days before and 56 hours after PG, followed by insemination, 16 hours after the second GnRH. Cows in 2PG-Ovsynch group (n=55) received two PGs, 14 days apart, followed by ovsynch program 12 days later. Cows in eCG-Ovsynch group (n=55) received eCG (500 IU, IM), 84 hours before ovsynch program. Pregnancy was confirmed 40 d after insemination by rectal palpation. Progesterone concentrations were evaluated at presynchronization, at the first GnRH and at PG of ovsynch program. <strong>RESULTS: </strong>The respective first service conception rates, days open and service per conception were similar between 2PG-ovsynch (45.45%; 94.0±6.30 days; 1.95) and eCG-ovsynch (40%; 90.1±4.58 days; 1.81) groups (p>0.05). The percentage of cows with high progesterone concentrations increased significantly from the first GnRH (38.2%) until PG (85.4%) in eCG-ovsynch group (p<0.05). The percentage of cows with high progesterone concentrations at the time of PG injection of ovsynch program was greater in eCG-ovsynch (85.4%) compared to 2PG-ovsynch (69%).<strong> CONCLUSIONS: </strong>Administration of eCG (500 IU), 84 hours prior to ovsynch could optimize timed breeding program biologically, practically and economically in lactating dairy cows.
eCG,ovsynch,presynchronization,prostaglandin,timed breeding
https://ijvm.ut.ac.ir/article_66098.html
https://ijvm.ut.ac.ir/article_66098_2409daad855a2f9ef6abd7aa3d5f7dae.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Passive protective effect of anti-K99 antibodies against enterotoxigenic E.coli infection in neonatal calves
97
107
EN
Khaleel
Alo
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
khaleelalo@ut.ac.ir
Gholamraza
Nikbakhat
0000-0002-2970-5827
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
nikbakht@ut.ac.ir
Samad
Lotfollahzadeh
0000-0002-6549-7922
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
samadlzadeh@ut.ac.ir
Farhad
Moosakhani
Department of Microbiology Sciences, Faculty of Veterinary Medicine, Branch of Karaj, Islamic Azad University, Karaj, Iran.
fmoosakhani@kiau.ac.ir
Adel
Gharabaghi
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
gharebaghy_ir@yahoo.com
10.22059/ijvm.2018.244346.1004855
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is the main causative agents of neonatal calf diarrhea. Passive transfer of antibodies that is derived from the dams of the calves through their colostrum plays an important role in disease prevention. OBJECTIVES: To determine the presence of natural specific antibodies against K99 antigen in dam’s serum and colostrum as well as in calf’s serum and examined the association between bacteriological and serological aspects for ETEC K99+ and calf diarrhea. METHODS: A specific PCR assay was used to detect K99-positive E. coli in fecal samples from two groups of normal and diarrheic calves. Specific antibodies against K99 fimbriae in dam serum, colostrum and calf serum were studied using indirect ELISA. RESULTS: Significant differences were observed between the antibody titer in normal and diarrheic groups. Significant differences were observed between normal and diarrheic calves in their serum anti-K99 titers (P =0.0005), their dams (P =0.0005) and colostrum (P =0.001), in which anti-K99 titer in dam’s serum and colostrum was higher in diarrheic group, but in calf’s serum was higher in normal group. CONCLUSIONS: Although anti-K99 antibody levels in diarrheic calves was less than normal calves, there is no correlation between the level of these antibodies and disease occurrence. This study suggests that anti-K99 antibodies do not solely promote immunity to ETEC infections.
calf diarrhea,Colostrum,enterotoxigenic Escherichia coli,Indirect ELISA,K99
https://ijvm.ut.ac.ir/article_65826.html
https://ijvm.ut.ac.ir/article_65826_c27bc127f427bc866842e0dd3dfbd37e.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Production of monoclonal antibodies against recombinant nucleoprotein of avian influenza virus, serotype H9N2
107
116
EN
Masoudreza
Seyfiabad Shapouri
Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
masoudrs@yahoo.com
Azadeh
Yektaseresht
0000-0003-1479-1723
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
azadeh_neisi@shirazu.ac.ir
Masoud
Ghorbanpoor Najafabadi
Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
mghorbanpour@yahoo.com
Amin
Jaydari
Department of Pathobiology, School of Veterinary Medicine, University of Lorestan, Khorram Abad, Iran
jaidariam@gmail.com
10.22059/ijvm.2018.245203.1004858
<strong>Background:</strong> Avian influenza viruses (AIVs) including the subtype H9N2 cause considerable financial losses to poultry industries. Rapid and accurate diagnosis of avian influenza (AI) infection is important in control and eradication programs. <strong>OBJECTIVES: </strong>The aim of this study was to produce monoclonal antibodies (MAbs) specific for the nucleocapsid protein )NP (of AIV H9N2 subtype to improve diagnostic assays. <strong>METHODS:</strong> Recombinant NP protein was expressed in Escherichia coli and purified using amylose resin chromatography column and used as an antigen for mice immunization. Spleen cells of the immunized mice were fused with SP2/0 myeloma cells. Next, culture supernatants of primary hybridoma clones were screened by indirect ELISA. After three rounds of sub cloning, the reactivity of the MAbs with recombinant and natural antigens was assessed by Western blotting. <strong>RESULTS:</strong> Six MAbs showed specific binding to recombinant and natural NP from AIV H9N2 in Western blot analysis, enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay. Cross-reactivity with genetically non-related including Newcastle viruse (Paramyxoviridae family) was not detected. <strong>CONCLUSIONS:</strong> Based on the results, the MAbs generated in this study could be used for the development of rapid diagnostic assays for recognition of AIV.
Antibody,avian,influenza,monoclonal,NP
https://ijvm.ut.ac.ir/article_66061.html
https://ijvm.ut.ac.ir/article_66061_9b4e073e03236567e85cc5ff79d3d03a.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Study of Blastocystis frequency among IBD patients referred to a gastroenterology center
117
124
EN
Sara
Mohammad Ali Gol
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
golsarah@yahoo.com
Sedigheh
Nabian
4151 2350 0002 0000
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
nabian@ut.ac.ir
Fatemeh
Arabkhazaeli
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
fateme1270@yahoo.com
Hamed
Mirjalali
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
hamed-mirjalali@hotmail.com
Saied
Bokaie
0000-0003-3505-0270
Department of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
sbokaie@ut.ac.ir
Mostafa
Rezaeian
Department of Parasitology and Mycology, Faculty of Health, Tehran University of Medical Sciences, Tehran, Iran
rezaiian@tums.ac.ir
Mohammad Reza
Zali
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
mrzali@ut.ac.ir
Hamid
Asadzadeh Aghdaei
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
hamidassadzade@yahoo.com
10.22059/ijvm.2017.241887.1004845
<strong>Background:</strong> <em>Blastocystis</em> is the most common anaerobic protozoa living in the large intestine of a broad spectrum of vertebrates. <strong>Objectives:</strong> The aim of this study was to investigate the <em>Blastocystis</em> infection rate in inflammatory bowel disease (IBD) patients.<strong> Methods: </strong>A total of 80 stool samples were collected from IBD-proved patients. All stool samples were cultivated in Dulbecco’s modified Eagle’s medium and checked by light microscopy for detection of <em>Blastocystis</em>. The Correlation between demographic data of IBD patients and <em>Blastocystis</em> was calculated using SPSS 23. <strong>Results:</strong> The enrolled patients comprised of 52 (65%) men and 28 (35%) women. The study showed <em>Blastocystis</em> in 16/80 (20%) of the samples by microscopic examination and culture method. The parasite was seen among 12 (23.08%) and 4 (14.29%) men and women, respectively. No statistically significant correlation was found between infection with the parasite and animal contact. Fisher’s exact test represented that there was no correlation between gender and the presence of <em>Blastocystis</em> (p value= 0.397). Fisher’s exact test denoted that there was no statistical correlation between age and the presence of the parasite (p value= 0.130). <strong>Conclusions:</strong> In this study, <em>Blastocystis</em> was found in 20% of enrolled patients who suffered from IBD. This infection rate was significantly higher than the studies have previously described <em>Blastocystis</em> in this group of patients.
Blastocystis,demographic data,inflammatory bowel diseases,microscopy,stool cultivation
https://ijvm.ut.ac.ir/article_65839.html
https://ijvm.ut.ac.ir/article_65839_ba6e74dac13203fc430e47c4d9f2f911.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
The use of methylphenidate for emergence from propofol and ketamine anesthesia in dogs
125
135
EN
Hadi
Imani Rastabi
0000-0002-1564-7240
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
h.imani@scu.ac.ir
Reza
Avizeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
r.avizeh@scu.ac.ir
Narges
Kavosi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
n_kavosi64@yahoo.com
Soroush
Sabiza
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
soroush-sabiza@yahoo.com
10.22059/ijvm.2018.238819.1004829
<strong>Background:</strong> Methylphenidate (MPH) has been used to induce emergence from general anesthesia. <strong>OBJECTIVES: </strong>The objective of the present study was to evaluate the effect of MPH on recovery from propofol and ketamine anesthesia in dogs. <strong>METHODS: </strong>Six healthy male mix-breed dogs weighing 21.9 ± 3.9 kg were used in a randomized crossover design. Thirty minutes after premedication with acepromazine (0.1 mg/kg; IM), anesthesia was induced with either IV propofol or ketamine (8 and 15 mg/kg, respectively). Dogs, six minutes after induction, received either IV normal saline or methylphenidate (1 mg/kg) (propofol-saline; propofol-methylphenidate; ketamine-saline; ketamine-methylphenidate). Each dog was anesthetized four times randomly with at least one week interval. <strong>RESULTS:</strong> No significant differences were observed between propofol-saline and propofol-methylphenidate as well as between ketamine-saline and ketamine-methylphenidate in the times needed for various chronological sequences of recovery (p>0.05). Recovery in the dogs that received methylphenidate was eventful and associated with some adverse effects. Heart rate showed a decrease in propofol-methylphenidate group compared to the base (p<0.05). Respiratory rate after administration of methylphenidate was more stable than that of saline. <strong>CONCLUSIONS:</strong> It was concluded that methylphenidate at 1 mg/kg could not shorten recovery time in the dogs premedicated with acepromazine and anesthetized with either propofol or ketamine. Testing lower doses of methylphenidate and using a different premedication agent are recommended for future studies.
anesthesia,emergence,ketamine,methylphenidate,propofol
https://ijvm.ut.ac.ir/article_65588.html
https://ijvm.ut.ac.ir/article_65588_bfda8676bddf9acd71fa5b8b6178fb4e.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Macroscopic and histopathological examinations of liver lesions in slaughtered cattle in Zabol City, Iran
135
144
EN
Abolghasem
Sanjari
Graduated from the Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
aboalghasem.sanjari@gmail.com
Seyedeh Ayda
Davari
Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
davari.aida1@gmail.com
Mehdi
Rasekh
Department of clinical Sciences, School of Veterinary Medicine, University of Zabol, Zabol, Iran.
mrasekh@gmail.com
10.22059/ijvm.2018.248683.1004869
<strong>Background:</strong><br />Liver lesions in cattle not only have negative effects on the cattle breeding<br />industry, but also lead to loss of animal protein production, and in particular<br />to endanger the consumers’ health.<strong> OBJECTIVES:</strong><br />This study was carried out to examine the macroscopic and histopathologic<br />lesions of liver in slaughtered cattle of Zabol City. <strong>METHODS:</strong><br />Livers of 281 cattle slaughtered at the industrial slaughterhouse of Zabol City<br />were collected from March to September 2015 for gross and microscopic<br />investigation. Tissue processing was conducted from specimens inspected to<br />tissue lesions with routine protocol and histopathological changes of livers<br />were examined under light microscopy. <strong>RESULTS:</strong><br />Macroscopic investigation of 281 cases showed 90 livers (32.02%) with gross lesions.<br />In histopathological examination, there were 83 cases (29.53%) with<br />hydatidosis, 3 cases (1.07%) with fasciolosis, 2 cases (0.71%) with<br />perihepatitis and 2 cases (0.71%) with lipidosis. <br /> <strong>CONCLUSIONS:</strong><br />Based on the results of this study, the most frequent liver lesion in cattle<br />slaughtered in Zabol City was hydatidosis indicating the active life cycle of <em>Echinococcus<br />guanulosus</em> and the outbreak of this parasitic disease in dogs and other<br />carnivores in Sistan region
Cattle,Histopathology,liver,macroscopic,slaughtered cattle
https://ijvm.ut.ac.ir/article_66137.html
https://ijvm.ut.ac.ir/article_66137_8cf620c6e91ba27d9b9b7bcfbd9147db.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Comparison of computerized digital and analog radiography for detection of bronchial pattern in dogs
145
152
EN
Amir
Tavakoli
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
tavakoli.amir.ir@gmail.com
Alireza
Vajhi
0000-0002-8048-7124
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
avajhi@ut.ac.ir
Mohammad
Molazem
0000-0003-4038-4849
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
mmolazem@ut.ac.ir
Sarang
Soroori
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
soroori@ut.ac.ir
Amir
Rostami
Department of Small Animal Internal Medicinel, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
arostami@ut.ac.ir
Mehdi
Hassankhani
Department of Internal Medicinel, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
mhassankhani@gmail.com
Davood
Faskhoodi
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
faskhoodi@ut.ac.ir
10.22059/ijvm.2018.226261.1004795
<strong>Background:</strong> Analog radiography has been replaced with digital radiography for canine diagnostic imaging in many veterinary clinics. There is no data comparing these modalities in a veterinary clinical setting to detect bronchial pattern signs in dogs. <strong>Objectives:</strong> In this study, computerized digital radiography (CR) and analog radiography were compared for diagnosis of bronchial pattern in dogs. <strong>Methods:</strong> Forty-five healthy (based on clinical examination and history taking) mixed breed dogs were divided into 3 age groups: up to two, two-six and more than 6 years old. Each group contained fifteen dogs. DR and FSR in right to left lateral (RL) and ventrodorsal (VD) views were taken. Two expert radiologists interpreted the radiographs based on counting bronchial ring and tram line signs in a double blinded scheme. <strong>Results:</strong> The statistical analysis of results, with Sign Test, shows that more bronchial ring and tram-like signs were counted with both radiologists using digital radiography. In addition, countable bronchial signs on right lateral position by digital and analog radiography were significantly more than in ventrodorsal view. In comparison with analog images, a greater number of bronchial ring and tram-like signs are associated with greater diagnostic confidence in digital modality. <strong>Conclusions:</strong> This study shows superior ability of digital radiography for detecting details in thoracic radiography of normal dogs in comparison with analog radiography. Since digital radiography is getting to be more commonly used by veterinary practitioners, it is necessary to understand the shortcomings of current classification of pulmonary pattern approach in digital radiology.
Analog,bronchial pattern,digital,Dog,Radiography
https://ijvm.ut.ac.ir/article_66062.html
https://ijvm.ut.ac.ir/article_66062_e7bab53b037d60482287d2542cb3228b.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Effect of fish oil supplementation and forage source on performance, rumen fermentation, nutrient digestion and chewing behaviour of Holstein bulls
153
166
EN
Hossein
Zakariapour Bahnamiri
Department of Animal Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
h.zakariapour@ut.ac.ir
Mahdi
Ganjkhanlou
Department of Animal Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
ganjkhanlou@ut.ac.ir
Abolfazl
Zali
Department of Animal Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
zalia@can.ut.ac.ir
Sara
Ataei Nazari
Department of Animal Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
saraattaee@ut.ac.ir
10.22059/ijvm.2017.241303.1004841
Background: Fat supplementation in ruminants ration often adversely affect dry matter intake (DMI), rumen fermentation and nutrients digestion. Basal diet ingredients is an undeniable determinant of ruminants responses including performance, chewing behaviour and nutrient digestibility to fat supplementation. Objective: Current study was conducted to evaluate the effect of Fish oil (FO) supplementation under different proportion of alfalfa hay (AH) and corn silage (CS) in ration on performance, rumen fermentation, nutrient digestibility and chewing behaviour of Holstein young bulls. Methods: Thirty six Holstein young bulls were used in a 2 × 3 factorial arrangement, with 2 levels of AH proportion (10 and 20 % of AH versus 20 and 10 % of dietary CS) combined with 3 levels of FO supplement (0, 1 and 2.1% of dietary dry matter). Calves were fed TMR consisting of 30 % of forage and 70 % of barley grain-based concentrate mix (dry matter basis). The experiment lasted for 90 days. Results: Dry matter intake was not affected by interaction of AH proportion and FO supplementation. Higher dietary CS proportion caused more DMI regardless of FO supplementation (8.71 versus 8.00 kg/d respectively for treatments with high and low CS proportion; P < 0.01). Highest level of FO reduced DMI regardless of AH proportion (8.65, 8.52 and 7.90 kg/d respectively for 0, 1 and 2.1 % of FO; P < 0.01). Rumen fermentation and nutrients digestibility were not affected by dietary treatments. Rumination times per kg of dry matter (DM) and physically effective neutral detergent fiber (peNDF) > 1.18 were increased in response to FO supplementation regardless of AH proportion (P < 0.01). High AH proportion showed lower total chewing activity and total rumination time (P < 0.01), higher chewing activity per kg of peNDF > 1.18 intake and eating times per kg of DM and peNDF > 1.18 intake (P < 0.01) regardless of FO supplementation. Conclusion: The results demonstrate that AH can be replaced by CS to prevent depression in DMI and stimulate chewing activity in the case of fat supplementation.
chewing behaviour,fat supplementation,forage type,nutrient digestibility,performance
https://ijvm.ut.ac.ir/article_65823.html
https://ijvm.ut.ac.ir/article_65823_e5f6b2a9011042e7c606086de9a66bfe.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Methicillin resistant Staphylococcus aureus (MRSA) in pastry cream products sold in Amol (Iran)
167
174
EN
Maryam
Azizkhani
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
azizkhani.maryam@gmail.com
Fahimeh
Tooryan
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
f.tooryan@umz.ac.ir
10.22059/ijvm.2018.239306.1004831
Abstract<br /> <br /> <strong>Background:</strong> Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) has been recognized as a matter of antibiotic resistance that is largely developed amongst common foodborne pathogens. MRSA is being considered as an important worldwide health threat and causes considerable concern to clinicians, food products manufacturers, governments and also consumers. <strong>OBJECTIVES:</strong> The objective of this study was to detect MRSA isolated from 360 samples of pastry cream products sold in the local markets in Amol, June 2016- May 2017, by plate count method and molecular technique. <strong>METHODS:</strong> The conventional plate counting method was conducted through inoculating appropriate dilutions of samples onto the Baired Parker Agar plates. MRSA isolates were detected by PCR method using mecA primers set. The resistance of isolated MRSA strains against some antibiotics was determined. <strong>RESULTS:</strong> Out of 360 pastry cream samples tested, 41.6% (150 samples) were contaminated by <em>S. aureus </em>with an average count of 4.94 log CFU/g in summer; 4.72 log CFU/g in autumn, 2.74 log CFU/g in winter and 3.62 log CFU/g in spring. Eleven samples out of 360 tested (3.05%) showed positive results for the mecA gene. No MRSA isolate was identified amongst winter samples. 56% of isolated strains showed sensitivity to oxacillin, 7% of isolates were sensitive to penicillin, 23 to ampicillin, 82% to gentamicin and 33% to tetracycline. <strong>CONCLUSIONS: </strong>According to the results, monitoring and improving the hygienic conditions of food production chain and educating food handlers and staff involved in food preparation is recommended in order to prevent MRSA prevalence.
Antibiotic,methicillin-resistant,pastry products,PCR,Staphylococcus aureus
https://ijvm.ut.ac.ir/article_66017.html
https://ijvm.ut.ac.ir/article_66017_0e9245b301bc4e78660db9e854a39bca.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
12
2
2018
06
01
Comparative histomorphometric study of the various segments of the spinal cord in the adult male and female mongoose (Herpestes edwardsii)
175
181
EN
Babak
Rasouli
0000-0002-9447-7429
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
babrs32@yahoo.com
Soghra
Gholami
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
gholami@shirazu.ac.ir
10.22059/ijvm.2018.235692.1004817
<strong><span style="text-transform: uppercase; font-size: 12pt;">Background:</span></strong><span style="font-size: 12pt;"> Anatomical and histological studies of the spinal cord have always garnered anatomists’ attention because of their high importance in various fields of veterinary medicine, zoology and behavioral science. <strong>OBJECTIVES:</strong> This work was conducted to understand the detailed histomorphometric aspects of the spinal cord of Indian gray mongoose (<em>Herpestes edwardsii</em>). <strong>METHODS:</strong> Six adult Indian grey mongooses in the terminal stages of disease and the status of approaching death were used in the present study. The spinal cords were dissected and fixed in 10% buffer formalin then paraffinized and sections of 6 μm thick were cut and stained with haematoxylin and eosin. In this study, the vertical and transverse diameters of the spinal cord, central canal, the length of the ependymal cells and the ratio of gray matter to white matter in the selected spinal cord segments, were measured with standard micrometric method using light microscope. <strong>RESULTS:</strong> In male and female mongooses, the longest transverse and vertical diameters of spinal cord segments were observed in the lumbar region. Although this stability and readability of the data were not seen in the transverse and vertical diameters of the central channel. Also, the highest ependymal cells in both sexes were observed in the lumbar region. <strong>CONCLUSIONS:</strong> It can be concluded that the transverse diameter of spinal cord is larger than the vertical one and the largest measured diameter and ratio of gray matter to white matter were identified in lumbosacral area.</span><br /> <span style="font-size: 12pt;"> </span>
comparative,histomorphomtery,mongoose,segments,spinal cord
https://ijvm.ut.ac.ir/article_65556.html
https://ijvm.ut.ac.ir/article_65556_ef182cf470e4d6fcf9982e86ffeba76c.pdf