University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Evaluation of the Hydroxyproline Content in the Frozen Allograft Tendon Impregnated with the Mesenchymal Cells & PRP in Lamb
1
9
EN
Rafid Naeem Majid
Alkhalifa
1Instructor at the University of Basra, Basra, Iraq. Post–graduate student at the Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
rafednaeem@yahoo.com
Davood
Sharifi
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
dsharifi@ut.ac.ir
Saied
Bokaie
0000-0003-3505-0270
Department of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
sbokaie@ut.ac.ir
Alireza
Bashiri
Department of Surgery and Radiology, Faculty of Veterinary Medicine .University of Tehran. Tehran. Iran.
alirezabashiri@ut.ac.ir
Hosein
Shad
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine .University of Tehran. Tehran. Iran
hshad55@yahoo.com
Rouhoulah
Esanejad
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine .University of Tehran. Tehran. Iran
hissanejhad@yahoo.com
Gholam Reza
Shams
4Department of Comparative Bio Sciences, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran
shams_ghr@ut.ac.ir
10.22059/ijvm.2019.256762.1004895
<strong>Background:</strong> Tendon grafting is mostly required to repair an injury to flexor tendon and due to the importance of this tendon; it needs methods to speed up the re-vitality of the tendon allograft.<br /> <strong>OBJECTIVES: </strong>The aim was to investigate the efficacy of the mesenchymal cells and platelet rich plasma on the hydroxyproline content of frozen allograft tendons after grafting in lamb.<br /> <strong>METHODS:</strong> In this experimental study, mid portion (five cm) of SDFT of fifteen lambs, from both forelimbs were removed and replaced with frozen allografts tendon. Animals were divided into 3 subgroups of 5 lambs each: control, PRP and MSCs. The hydroxyproline concentration was measured by after 60 days. Data was analyzed using student t test at <em>P</em><0.05%.<br /> <strong>RESULTS: </strong>The normal mean values of hydroxyproline content was 137.171±5.291 mg/g dry matter which 87.694±6.502 in control group but 99.694±1.839 in PRP group and in group treated with MSC was recorded 134.322±2.123 mg/g dry matter (<em>P</em><0.05%) it was quite significantly different between control tendons with that of normal one of the same animal. There was marked increase in hydroxyproline content of MSC group when compare with that of control and PRP groups.<br /> <strong>CONCLUSIONS: </strong>The results of this study showed that MSCs could enhance HP content in the frozen grafted tendon in lamb.
Frozen tendon,Hydroxyproline,Lamb,Mesenchymal cell,PRP
https://ijvm.ut.ac.ir/article_69865.html
https://ijvm.ut.ac.ir/article_69865_50c97f318018912e29ca7c4d4e18c78b.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse
11
25
EN
Bassim
Khashjoori
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
bassem@ut.ac.ir
Seyed Mehdi
Ghamsari
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2Institute of Biomedical Research, University of Tehran, Tehran, Iran
ghamsari@ut.ac.ir
Mohammad Mehdi
Dehghan
0000-0002-8825-9070
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2Institute of Biomedical Research, University of Tehran, Tehran, Iran
mdehghan@ut.ac.ir
Saeed
Farzad Mohajei
0000-0003-4694-347X
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2Institute of Biomedical Research, University of Tehran, Tehran, Iran
saeedfarzad@ut.ac.ir
Hossein
Gholami
2Institute of Biomedical Research, University of Tehran, Tehran, Iran
hgholami@ut.ac.ir
Hananeh
Golshahi
3Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
hgolshahi@ut.ac.ir
10.22059/ijvm.2019.244278.1004854
<strong>Background:</strong> Equine wounds, particularly those involving the distal portion of the limbs, often undergo prolonged complex healing and may enter a non-healing state with obvious financial and welfare implications. Poor blood supply and lower levels of oxygen to the lower limbs lead to imbalance of growth factors related to wound healing. Decellular amniotic membrane has been widely used in soft tissue engineering especially skin regeneration due to its biological and mechanical properties. It is used as a scaffold for treatment of partial full thickness wound dressing.<br /> <strong>OBJECTIVES:</strong> Evaluation of the effects adipose derived mesenchymal cells (ADMSCs) cultured on decellularized amniotic membrane (DAM) in wound healing of distal part of equine limb.<br /> <strong>METHODS:</strong> Stem cells were isolated from adipose tissue in the base of the tail and then cultured and seeded on bovine decellular amniotic membrane. Full thickness excision wounds (2x3 cm) were created on the mid-lateral of all metatarsus of each horse aseptically. The wounds were classified into four groups: the first one covered by DAM seeded by ADMSCs, the second one dressed with only DAM, the third one treated with1% silver sulfadiazine, and the last one as control with no treatment. Digital photographs of wounds were obtained at each bandage change while biopsy samples were obtained for histopathology at days 5, 15 and 30 after treatment.<br /> <strong>RESULTS:</strong>Wounds treated with DAM/ADMSCs significantly demonstrated promotion of wound healing in geometric analysis of total wound area, epithelialization and wound contraction compared with the other groups (<em>P</em><0.05). Microscopically, re-epithelization of wounds covered by DAM/ADMSCs and DAM at day 5 was significantly more than Control group (<em>P</em><0.05). Fibroplasia in wounds treated with DAM/ADMSCs and DAM was higher than the group that received sulfadiazine (<em>P</em><0.05).<br /> <strong>CONCLUSIONS: </strong>These results indicate positive effects of using DAM to improve the quality and speed of wound healing. Use of DAM as a scaffold for ADMSCs shows better results.
Amniotic membrane,Distal limb,Horse,Mesenchymal stem cells,Wounds healing
https://ijvm.ut.ac.ir/article_70105.html
https://ijvm.ut.ac.ir/article_70105_05e533f2747e1de5c31fb281900a6f25.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Detection and Molecular Characterization of Avian Rotaviruses in Broiler Farms in Guilan
27
36
EN
Fatemeh
Poursafar
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
poursafar.f@gmail.com
Vahid
Karimi
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
vkarimi@ut.ac.ir
Arash
Ghalyanchi Langeroudi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
arashghalyanchi@gmail.com
Hossein
Hosseini
0000-0003-2696-621X
Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, IRAN.
hosseini.ho@gmail.com
Fatemeh
Mousavi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
fatememousavi7715@gmail.com
10.22059/ijvm.2018.265109.1004919
<strong>Background:</strong> Avian rotaviruses are one of the important agents of enteritis in birds and have a worldwide distribution. These viruses have an important role in runting and stunting syndrome in chickens.<br /> <strong>Objectives:</strong> The aim of this study was detection and molecular characterization of avian rotaviruses in broiler farms in Guilan province.<br /> <strong>Methods:</strong> In this study, 100 samples were collected on different farms from the intestines of healthy and diseased broiler chickens and tested by RT-PCR targeting of the NSP4 gene. Sequencing of NSP4 gene has been used for characterization of detected viruses.<br /> <strong>ResultS: </strong>Avian rotaviruses were detected in 46% of samples by RT-PCR. Partial sequencing of seven NSP4 genes and phylogenetic analysis indicated that the viruses belonged to group A avian rotaviruses and were 97.52% to 100% homologous. Sequence identity between viruses with previous chicken rotavirus sequences was 31.68% to 99.5%. In comparison with turkey rotavirus Ty-1 and pigeon rotavirus, the homology was 76.98% to 78.22% and 74.75% to 76.98%, respectively. Evident differences were detected between the rotaviruses studied and human and pig rotaviruses with homologies of 31.19% to 38.86% for pig and 47.77% to 48.02% for human viruses.<br /> <strong>Conclusions:</strong> This study was the first molecular characterization of avian rotaviruses in Iran. Results of this study showed that the group A avian rotaviruses are one of the considerable enteric pathogens in broiler chickens of Guilan province. Further study on the whole genome structure could elucidate the evolutionary process of avian rotaviruses circulating in Iran.
Avian rotaviruses,Broiler Chickens,Iran,NSP4 gene,Phylogenetic study ____________________________________________________________
https://ijvm.ut.ac.ir/article_70049.html
https://ijvm.ut.ac.ir/article_70049_573ce90b84a553ed0553079b01c84561.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Ultrasonographic Liver Findings in a Sheep Flock Involved in Chronic Fasciolosis
37
43
EN
Siamak
Alizadeh
0000-0003-1112-7026
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
s_alizadeh01@yahoo.com
Tohid
Mohammadi
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
dr.mohammadi82@yahoo.com
10.22059/ijvm.2018.254143.1004888
<strong><span style="color: windowtext; text-transform: uppercase; font-size: 12pt;">Background:</span></strong><span style="color: windowtext; font-size: 12pt;"> <em>Fasciola hepatica</em> is a genus of the trematodes which mainly affects the liver of sheep and these livestock are accidentally infected by eating plants or drinking water contaminated with the metacercariae of this parasite. These parasites can cause considerable economic damage by destroying the liver and bile ducts.</span><br /> <strong><span style="color: windowtext; font-size: 12pt;">OBJECTIVES:</span></strong><span style="color: windowtext; font-size: 12pt;"> The aim of this study was to evaluate the role of ultrasonographic diagnosis in chronic hepatic fasciolysis of sheep and to provide ultrasonography views of fasciolosis in sheep’s liver.</span><br /> <strong><span style="color: windowtext; font-size: 12pt;">METHODS:</span></strong><span style="color: windowtext; font-size: 12pt;"> In this cross-sectional study, 256 sheep of the Makoei breed, including 130 male (50.8%) and 126 female (49.2%) with diagnosis of chronic fasciolosis confirmed by the appearance of fluke eggs in feces and using enzyme-linked immunosorbent assay (ELISA) were referred to the Department of Radiology and Ultrasonography, Doppler ultrasound was performed. Average age of sheep was 2.1 years (minimum age 7 months and maximum age 6 years). Data were analyzed by SPSS software (version 11.5) and the statistical test was chi-square test. P value less than 0.05 was considered significant.</span><br /> <strong><span style="color: windowtext; font-size: 12pt;">RESULTS:</span></strong><span style="color: windowtext; font-size: 12pt;"> In the results, 55.6% of sheep had ascites in the abdominal cavity and in 5.7% and 11.3%, bile ducts and gallbladder were affected, respectively, and in 4.8% of sheep both the liver and the bile ducts were involved. In 22.6% of sheep, there was no abnormal finding in ultrasound. The most common site of injury was in the posterior part of the right lobe of liver. There was no significant difference between the place of involvement in the liver, age and sex of the patients.</span><br /> <strong><span style="color: windowtext; font-size: 12pt;">CONCLUSIONS: </span></strong><span style="color: windowtext; font-size: 12pt;">According to this study, ultrasonography can play an important role in the diagnosis and follow up of sheep’s chronic fasciolosis treatment.</span>
Diagnosis,Fasciolosis,Liver,sheep,Ultrasonography
https://ijvm.ut.ac.ir/article_69784.html
https://ijvm.ut.ac.ir/article_69784_32a526de9182b9fc3000a2058236a58a.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Genetic Characterization of Argas persicus From Iran by Sequencing of Mitochondrial Cytochrome Oxidase I (COX1) and 16s rRNA Genes
45
57
EN
Shahriar
Yavari
Department of Parasitology, Iranian Center for Ticks and Tick-borne Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
shahriar.yavari@yahoo.com
Sedigheh
Nabian
Department of Parasitology, Iranian Center for Ticks and Tick-borne Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
yavarishariyar@gmail.com
Elahe
Ebrahimzade Abkooh
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
eebrahimzade@um.ac.ir
Parviz
Shayan
0000-0002-4666-3235
Department of Parasitology, Iranian Center for Ticks and Tick-borne Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
pshayan@ut.ac.ir
Hamidreza
Shokrani
0000-0001-5692-3718
Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
hamidreza_shokrani@yahoo.com
10.22059/ijvm.2019.266128.1004926
<strong>Background:</strong> <em>Argas persicus</em> has a great importance for health and veterinary, it can transmit many infectious agents such as <em>Borrelia anserina</em> (avian spirochetosis) and <em>Aegyptianella pullorum</em>. Distinguishing Argasidae due to close morphological relationship is difficult.<br /> <strong>OBJECTIVES:</strong> In the present study, we performed molecular analyses based on PCR and sequencing of Amplicon derived from 16S rRNA and COX1 genes of <em>A. persicus</em> specimens in several provinces of Iran. <br /> <strong>Methods:</strong> Out of seventy <em>Argas persicus</em> collected and confirmed morphologically, eight ticks were chosen from five provinces of Iran for gene analysis. Their DNA were extracted and amplificated using primers derived from 16 S ribosomal RNA and COX1 genes using PCR. Then the amplicons were sequenced and analyzed by Chromas software and sequence alignment program (Clustal W). Phylogenetic analysis was also conducted using MEGA ver. 6.06 with a maximum-likelihood method.<br /> <strong>RESULTS:</strong> Sequencing results indicated that all eight samples belonged to <em>A. persicus</em> species. Their nucleotide sequencing revealed that the interspecific sequence differences of both genes (16S rRNA genes and COX1) between our isolates were very infrequent. All isolates from different provinces were conserved across regions except for one isolate that exhibited a difference of only 1 nucleotide. Within Phylogenetic tree, <em>A. persicus</em> formed a clade with <em>A. persicus</em> from other regions of the world (South Arica, Italy, China, and South Australia).<br /> <strong>CONCLUSIONS:</strong> Our findings suggested a very close phylogenetic relationship between <em>A. persicus</em> specimens obtained from different regions of Iran.<br /> <strong>Keywords:</strong>
Argas persicus,COX1,Phylogenetic analysis,16S rRNA
https://ijvm.ut.ac.ir/article_70106.html
https://ijvm.ut.ac.ir/article_70106_a12973d676a912d843d5612ba9978c8c.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Molecular Characterization and Phylogeny Analysis Based on Sequences of Cytochrome Oxidase gene From Hemiscorpius lepturus of Iran
59
67
EN
Abbas
Jolodar
Department of Basic Sciences, Biochemistry and Molecular Biology Section, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
jolodara@yahoo.com
10.22059/ijvm.2018.245802.1004883
<strong>Abstract:</strong><br /> <strong>Background:</strong> <em>Hemiscorpius lepturus</em> is a medically important scorpion found along the Iranian borders, especially near to Khuzestan Province in the south-west of Iran. This is the only non-buthid scorpion which is potentially lethal in southern Iran and is responsible for severe dermonecrotic scorpionism.<br /> <strong>OBJECTIVES: </strong>In this study, DNA fragment of the mitochondrial cytochrome c oxidase subunit I (COXI) gene of <em>H. Lepturus</em> for the molecular phylogenetic analysis was amplified.<br /> <strong>METHODS:</strong> We amplified a 624 bp gene fragment of cytochrome C oxidase subunit I (COXI) from <em>H. lepturus</em> collected from Khuzestan, Ahvaz by PCR. After sequencing of the PCR products, the phylogenetic analysis was performed using the neighbor-joining method with 1000 replicates of bootstrapping using the MEGA7 software.<br /> <strong>RESULTS: </strong>The results of phylogenetic analysis revealed four distinct clusters (A1, A2, B and C) belonging to the family of Hemiscorpionidae that were grouped together with bootstrap score between 77-96%. The gene fragment of Hl-Kh formed a cluster relative to the only scorpion of <em>H. Lepturus</em> (Hl) from Izeh, Iran with a good bootstrap score of 96. These two samples isolated from the Khuzestan province of Iran are closely related, as they clustered together as Cluster B. The genetic distances of Hl-Kh among the cluster A1, A2 and C ranged from 16 to19% and the lowest interspecific distance was in cluster B between Hl-Kh and Hl (6%).<br /> <strong>CONCLUSIONS:</strong> Hl-Kh isolated from the Khuzestan province of Iran are closely related to Hl, as they showed the greatest interspecific variation observed in Hemisccorpius genus in this study. Although the sample size in Cluster B is not large enough to draw a final conclusion, the percentage of sequence divergence was high enough for interspecific comparisons to provide separation of species.
Hemiscorpius lepturus,Scorpion,phylogenetic,Cytochrome C oxidase subunit 1,Venom
https://ijvm.ut.ac.ir/article_69954.html
https://ijvm.ut.ac.ir/article_69954_57815f6b2aa9b0bf394cfd81f07fbddb.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Hypothyroidism effects on the morpho-histometrical features of testes in adult rats
69
77
EN
hero
Sheikholeslami
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
mahabad9160@gmail.com
Farzam
Sheikhzadeh
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
fsheikhzadeh@tabrizu.ac.ir
Seyed Mahdi
Banan Khojasteh
1Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
smbanan@tabrizu.ac.ir
Nazli
Khajehnasiri
2Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran
n_khajehnasiri@sbu.ac.ir
Ali
Dastranj
3Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
dastranjt@yahoo.com
Hassan
Morovvati
0000-0003-0275-1636
4Department of Basic Sciences, School of Veterinary Medicine, University of Tehran, Tehran, Iran
hmorovvati@ut.ac.ir
10.22059/ijvm.2018.260974.1004909
<strong>Background:</strong> Up until now, the effect of thyroid hormones on the male reproductive organ, after puberty, is not well understood.<br /> <strong>OBJECTIVES: </strong>In the present study, the morpho-histometrical effects of thyroid hormone deficiency on the adult-male rat reproductive organs were investigated.<br /> <strong>MATHODS: </strong>Thirty adult male rats were randomly divided into three groups (n=10): control, hypothyroid, and hypothyroid which receives Levothyroxine. After 4-months we examined the number of spermatogonia cells, primary spermatocytes, and Sertoli cells, as well as the seminiferous tubules epithelium thickness, area of seminiferous tubules, and the diameter and thickness of testis artery walls.<br /> <strong>RESULTS:</strong> No significant difference was observed between the hypothyroid and control rats in terms of the area of seminiferous tubules, the number of spermatogonia cells, primary spermatocytes, and Sertoli cells. Moreover, the epithelium thickness of seminiferous tubules and the diameter and thickness of artery walls were significantly decreased in hypothyroid group compared with the control rats.<br /> <strong>CONCLUSIONS:</strong> These observations are consistent with the hypothesis that hypothyroidism can affect the male fertility and declare that it may influence male reproduction by reducing the testis blood flow.<br />
Artery,Hypothyroidism,Male reproductive cells,Seminiferous tubules
https://ijvm.ut.ac.ir/article_69976.html
https://ijvm.ut.ac.ir/article_69976_dbfa3cdee87b0c9b65d5f90609cd56ac.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
TiO2 Nanoparticles as a Common Component of Sunscreens: An Experimental Study of Dermal/Ocular Safety Assessment
79
86
EN
Seyedeh Maedeh
Jafari
Department of Comparative Biosciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
shahroozian@semnan.ac.ir
Goudarz
Sadeghi Hashtjin
Department of Comparative Biosciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
gsadeghi@ut.ac.ir
Mohammad Kazem
Koohi
Department of Comparative Biosciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
mkkoohi@ut.ac.ir
Ali
Rasooli
0000-0001-6591-2404
Department of Comparative Biosciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
arasooli@ut.ac.ir
Ebrahim
Shahroozian
Department of Basic Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
shahroozian@gmail.com
10.22059/ijvm.2018.257860.1004898
<strong>Background:</strong> The safety evaluations of sunscreens containing Titanium Dioxide-Nanoparticles (TiO2-NPs) were done by dermal exposure, acute dermal and eye irritation/corrosion, and skin sensitization according to the guideline for Organization for Economic Co-operation and Development (OECD).<br /> <strong>OBJECTIVES: </strong>The aim of our study was the evaluation of safety and toxicity of TiO2-NPs following acute sunscreen exposure.<br /> <strong>METHODS: </strong>TiO2 and TiO2-NPs (20-40 nm and 98% purity) were purchased in the anatase crystal phase, and five types of concentration for sunscreens were made which were carried out in five different treatment groups in mice and rabbits.<br /> <strong>RESULTS:</strong> In acute eye irritation using rabbits, the only irritation effect was observed in the conjunctivae area within one hour after administration both in TiO2-NPs group and TiO2-Ps. In acute dermal irritation using rabbits did not show a significant difference among groups in different concentrations and durations. Similarly, in a skin sensitization test using mice, contact hypersensitivity (CHS) did not show a significant difference (<em>P</em><0.05) among groups in 15% concentration of TiO2 in the different durations after application.<br /> <strong>CONCLUSIONS:</strong> Our finding demonstrates that TiO2-NPs and TiO2-Ps in sunscreens are relatively safe and did not induce statistically significant eye and dermal irritation and skin hypersensitivity
Dermal irritation,Eye irritation,OECD,Skin sensitization,TiO2-NPs
https://ijvm.ut.ac.ir/article_70012.html
https://ijvm.ut.ac.ir/article_70012_d7ee735ba1e6cd22ff6766364fe3ff46.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
In vitro Scolicidal Effect of Ginger (Zingiber officinale Roscoe) Ethanolic Extract Against Protoscolices of Hydatid Cyst
87
99
EN
Elham
Houshmand
0000-0002-2282-5068
1Department of Parasitology, Faculty of Veterinary Medicine, Rasht Branch, Islamic Azad University, Rasht, Iran
drehooshmand@iaurasht.ac.ir
Hanieh Sadat
Kamalifar
2Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
hani_k72919@yahoo.com
Hossein
Elmi
3Department of Vet Bacteriology, Rasht Branch, Islamic Azad University, Rasht, Iran
hosseinelmi1373@gmail.com
10.22059/ijvm.2018.263561.1004915
<strong>Background:</strong> To prevent the recurrence of hydatid cysts after surgery, it is essential to use effective scolicidal agents. Most of these agents are not safe due to their undesired side effects. Recently, studies have been conducted to find natural scolicidal agents with more efficacies and low side effects.<br /> <strong>OBJECTIVES:</strong> In this study, the in vitro scolicidal effect of ethanolic extract of ginger (<em>Z.officinale</em>) on protoscolices of hydatid cyst was investigated.<br /> <strong>METHODS: </strong>A certain number of protoscolices (about 500) were treated with various concentrations of ginger ethanolic extract (40, 50, 100, 150 and 200 mg/ml) and the effect of each concentration was evaluated for specified time periods (15, 30, 45 and 60 minutes). Each concentration/time was performed in triplicate and the viability of protoscolices was confirmed by 0.1% eosin staining.<br /> <strong>RESULTS:</strong> The mortality rate with ethanolic extract of <em>Z.officinale</em> after 60 minutes was as follows: 68%, 92.3%, 93% and 100% at 50, 100, 150 and 200 mg/ml, respectively, and there was no significant difference between the three concentrations of 200, 150 and 100 mg/ml (<em>P</em>>0.05). However, at the concentrations of 200 mg/ml after 30 minutes of incubation, 100% protoscolices were dead. Based on Tukey’s test, a significant difference in the percentage of live protoscolices was found between the different concentrations of ginger extract with the exception of between 150 and 100 mg/ml concentrations (<em>P</em>>0.05).<br /> <strong>CONCLUSIONS:</strong> Ethanolic extract of <em>Zingiber officinale</em> had a high scolicidal activity in vitro, and it has the potential to be used as a scolicidal agent in the surgical treatment of hydatid cysts.
Ethanolic extract,Ginger,Hydatid cyst,in vitro,Scolisidal effect
https://ijvm.ut.ac.ir/article_69975.html
https://ijvm.ut.ac.ir/article_69975_160f1a3d73f86b48fc23bc1640d8bf6b.pdf
University of Tehran
Iranian Journal of Veterinary Medicine
2251-8894
2252-0554
13
1
2019
01
01
Mislabeling in Cooked Sausage is a Seriously Increasingly Problem in Food Safety
101
113
EN
Tareq
Al-Qassab
Department of Food Hygiene, Faculty of veterinary Medicine, University of Tehran
tarikmahdi.110@ut.ac.ir
Abolfazl
Kamkar
0000-0003-4001-2922
Department of Food Hygiene, Faculty of veterinary Medicine, University of Tehran
akamkar@ut.ac.ir
Parviz
Shayan
0000-0002-4666-3235
Department of Pathophysiology, Faculty of Veterinary Medicine University of Tehran
Research Institute Molecular Biological System Transfer, Iran Tehran (MBST)
pshayan@ut.ac.ir
Ali
Khanjari
Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran
akhanjari@ut.ac.ir
10.22059/ijvm.2018.267894.1004935
<strong>Background:</strong> Identifying the animal species origin in meat and meat products is important for preventing adulteration and protecting consumers in terms of health and religious convictions. Species-specific polymerase chain reaction (PCR) is known as a suitable method for identifying meat species.<br /> <strong>OBJECTIVES:</strong> This study aimed to use a species-specific PCR assay for the detection of mislabeling in cooked sausage meats as adulterants by use of multiplex PCR.<br /> <strong>METHODS:</strong> A total of 114 samples including sausage labeled containing 40%, 55% and 70% red meat of 10 different brands were collected from various markets and supermarkets. Following genomic DNA extraction from cooked sausages which were claimed to be made of red meat, multiplex PCR was performed to detect adulteration in processed food.<br /> <strong>RESULTS:</strong> According to the analysis, 60 sausage samples showed that they consist of only meat from chicken (52.6%), 48 sausage samples consist of meat from beef and chicken (42.1%) and only 5.3% of the examined sausages were prepared with the meat of beef (6 samples).<br /> <strong>CONCLUSIONS:</strong> This high rate of undeclared chicken meat in sausage samples is most probably due to achieving more profit. Our results indicated that the meat species substitution occurs often in processed meats like sausages, which indicates the need of more governmental controls.
Adulteration,Mislabeling,multiplex PCR,Polymerase Chain Reaction (PCR),Sausage
https://ijvm.ut.ac.ir/article_69843.html
https://ijvm.ut.ac.ir/article_69843_cbe04ecd6e2dfaa8b173b12d4893306f.pdf