TY - JOUR ID - 19607 TI - Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins JO - Iranian Journal of Veterinary Medicine JA - IJVM LA - en SN - 2251-8894 AU - Nikbakht Brujeni, Gholam Reza AU - Tabaabaei, Saeid AU - Khormali, Mahmood AU - Ashrafi, Iraj AD - دانشکده دامپزشکی Y1 - 2009 PY - 2009 VL - 3 IS - 1 SP - EP - DO - 10.22059/ijvm.2009.19607 N2 - Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion, immunoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion and immunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of mammalian species. Anti-camel antibodies bound camel IgG1, IgG2, IgG3 subclasses like the subclasses of sheep, but no reactivity to all subclasses chains in cattle and horse antibodies were observed. Polyclonal anti-camel IgY was specifically light chain reactive. Overall, the potential of using egg yolk immunoglobulins as a convenient source of anti antibodies to camel immunoglobulins was demonstrated. UR - https://ijvm.ut.ac.ir/article_19607.html L1 - https://ijvm.ut.ac.ir/article_19607_7d6b01229e83471ca54fa84e0db120a5.pdf ER -