TY - JOUR ID - 24618 TI - A novel chemical method for measuring ketone bodies in subclinical ketosis JO - Iranian Journal of Veterinary Medicine JA - IJVM LA - en SN - 2251-8894 AU - Gholampour, Hadi AU - Asadian, Peyman AU - Erfanmanesh, Ahmad AU - Jahantigh, Mahdi AD - گروه پژوهشی فرآورده‌های بیولوژیک دامی، جهاد دانشگاهی، واحد تهران AD - گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه لرستان AD - گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه زابل Y1 - 2012 PY - 2012 VL - 6 IS - 1 SP - 7 EP - 11 DO - 10.22059/ijvm.2012.24618 N2 - Backgrounds: Subclinical ketosis can cause greater economic loss due to a lack of clinical symptoms. Objectives: The present study was aimed to design a chemical method for measuring serum ketone bodies in the affected subclinical cows. Methods: Acetoacetate concentrations were measured using a nitroprusside reaction and b-hydroxy butyric acid (BHBA) which was oxidized to acetoacetate using nanosilver particles to determine its concentration. Recovery tests were done for different concentrations of betahydroxy butyrate in bovine pooled serum. Results: Actoacetate levels were in range of 0.1-6 mM, and the values for BHBA were found to be in the range of 0.125-3 mM. Conclusions: It was concluded that this technique can be considered as a simple method for measuring ketone bodies in biological fluids. Backgrounds: Subclinical ketosis can cause greater economic loss due to a lack of clinical symptoms. Objectives: The present study was aimed to design a chemical method for measuring serum ketone bodies in the affected subclinical cows. Methods: Acetoacetate concentrations were measured using a nitroprusside reaction and b-hydroxy butyric acid (BHBA) which was oxidized to acetoacetate using nanosilver particles to determine its concentration. Recovery tests were done for different concentrations of betahydroxy butyrate in bovine pooled serum. Results: Actoacetate levels were in range of 0.1-6 mM, and the values for BHBA were found to be in the range of 0.125-3 mM. Conclusions: It was concluded that this technique can be considered as a simple method for measuring ketone bodies in biological fluids. UR - https://ijvm.ut.ac.ir/article_24618.html L1 - https://ijvm.ut.ac.ir/article_24618_dab0442a5b3d7ab283b543ee6d1d41a4.pdf ER -