TY - JOUR ID - 32017 TI - Distribution of virulence associated genes in isolated Escherichia coli from avian colibacillosis JO - Iranian Journal of Veterinary Medicine JA - IJVM LA - en SN - 2251-8894 AU - Kafshdouzan, Khatereh AU - Zahraei Salehi, Taghi AU - Nayeri, Bahar AU - Madadgar, Omid AU - Yamasaki, Shinji AU - Hinenoya, Atsushi AU - Yasuda, Nouritomo AD - Department of Microbiology, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran AD - Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan Y1 - 2013 PY - 2013 VL - 7 IS - 1 SP - 1 EP - 6 KW - Avian pathogenic Escherichia coli KW - multiplex PCR KW - virulent factors DO - 10.22059/ijvm.2013.32017 N2 - BACKGROUND: Colibacillosis is one of the most prevalentdiseases in the world that causes multimillion-dollar annuallosses. OBJECTIVES: In order to evaluate molecular epidemiologyof some virulence associated factors in Escherichia coli,isolated from poultry, the presence of iut A, iss, hly F, omp T, iroN, afa, sfa (S)and pap G (II) were investigated by multiplex PCRassay. METHODS: Two hundred thirty four Escherichia coliisolated from avian colibacillosis (APEC) and fifty four fecal E.coli isolates from the feces of apparently healthy birds (AFEC)were investigated for presence of some virulence associatedgenes by two panel of multiplex PCR. Statistical analysis wasperformed using |c2 test. the p-value was |£|0.05. RESULTS:Among 234 E. coli strains associated with colibacillosis and 54AFEC strains, 85% of isolates were positive for at least one of thevirulence gene. The three most prevalent genes in E. coli isolatedfrom colibacillosis were hly F (77.3%), omp T(73%) and iss(68.2%). Iut A, iro Nand pap G (II) were detected in 157 (67.4%),152 (65.2%) and 41(17.6%) respectively. None of isolatesharbored sfa (s) and afa genes. Several combination patterns ofvirulence genes were detected. Combination of hly F, omp T(70.8%) was the most prevalent pattern. CONCLUSIONS: theprevalence of iss, hly F, omp T, iro N genes in APEC isolates wassignificantly more than AFEC strains and probably these genesplay an important role in the pathogenesis of APEC strains. UR - https://ijvm.ut.ac.ir/article_32017.html L1 - https://ijvm.ut.ac.ir/article_32017_8437796cc482a67e5069d1e45c2af869.pdf ER -