TY - JOUR ID - 61638 TI - Evaluate Toxocara Canis Excretory-Secretory Antigens in Experimental Allergic Encephalomyelitis (EAE) JO - Iranian Journal of Veterinary Medicine JA - IJVM LA - en SN - 2251-8894 AU - Borhani zarandi, Mehdi AU - Hoseini, Seyed Hosein AU - Jalousian, Fatemeh AU - Etebar, Fazeleh AU - Vojgani, Mohamad AD - , Department of Parasitology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran AD - Department of Parasitology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran AD - Department of Parasitology, faculty of Veterinary medicine, Tehran University, Tehran, Iran AD - Ph.D., Department of Immunology and Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Y1 - 2017 PY - 2017 VL - 11 IS - 2 SP - 175 EP - 183 KW - disability score KW - EAE gene expression KW - experimental allergic encephalomyelitis KW - immune system in EAE Toxocara canis excretory- secretory antigens DO - 10.22059/ijvm.2017.61638 N2 - BACKGROUND: Toxocara canis is the most prevalent intestinal roundworm of canid species. OBJECTIVES: This study aims to evaluate the effects of Toxocara canis excretory-secretory antigens (TcES Ag) on modulating the immune system in Experimental Allergic Encephalomyelitis (EAE) model. METHODS: Adult worms of T.canis were collected from dogs to obtain excretory-secretory antigens. Female C57BL/6mice divided to four groups (5 mice in each) including: group 1 (MOG +TcES Ag), 2 (MOG), 3 (normal) and 4 (TcES Ag). EAE was induced in groups 1 and 2 using myelin oligodendrocyte glycoprotein peptide. Before EAE induction, TcES Ag was injected in group 1. Twenty nine after EAE induction, mice spleens were removed. Mononuclear cells were cultured and used for RNA extraction.Real Time PCR was performed to evaluate RNA expression levels of T-bet (Th1 lineage-specific transcription factor), GATA-3 (Th2 transcription factor), and FOXP3 (Treg transcription factor). RESULTS: Our results indicated the clinical signs (disability score) of mice in group 1 significantly were decreased as compared to the control group. Gene expression of T-bet in the TcES Ag treatment groupmarkedly were diminished compared to MOG and normal group. The expression of GATA-3 gene in group 1 was lower than that in group 2. CONCLUSIONS: It seems that the TcES Ag may reduce disability score in multiple sclerosis EAE model, and other recombinant antigens should be examined. UR - https://ijvm.ut.ac.ir/article_61638.html L1 - https://ijvm.ut.ac.ir/article_61638_c64c34abe398a08a7aafd21475d7d05a.pdf ER -