TY - JOUR ID - 67233 TI - Comparison of the Effect of Bovine Platelet Lysate and Platelet Rich Plasma as Growth Promoters on Growth Rate and Viability of Different Cell Lines JO - Iranian Journal of Veterinary Medicine JA - IJVM LA - en SN - 2251-8894 AU - Erfanmanesh, Ahmad AU - Nikaein, Donya AU - Mohajerfar, Tahereh AD - Academic Center for Education, Culture and Research (ACECR), Tehran Organization, Tehran, Iran AD - Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Y1 - 2018 PY - 2018 VL - 12 IS - 3 SP - 249 EP - 258 KW - Cattle KW - fetal bovine serum KW - fetal bovine serum alternatives KW - platelet lysate KW - platelet rich plasma DO - 10.22059/ijvm.2018.250527.1004872 N2 - Background: Cell therapy and cell culture have received much attention in recent decades. Suitable cell growth requires growth supplements such as fetal bovine serum (FBS). FBS is component rich in nutrients, growth factors and supplementary compounds. However, FBS utilization has some limitations including mass production. Therefore, finding alternatives with the same growth promoting effects is inevitable. OBJECTIVES: This study was designed to compare the effect of bovine platelet lysate (PL) and PRP on different cell lines as a cost effective and available alternative for FBS. METHODS: Three conventional cell lines were investigated. Protein pattern of PL and platelet rich plasma (PRP) in comparison to FBS was determined using SDS page electrophoresis, and MTT and plating efficiency of cell lines in presence of PL and PRP were evaluated. RESULTS: The results demonstrated that platelet rich plasma and platelet lysate could increase cells’ viability similar to FBS. These results were significant in comparison with control group. CONCLUSIONS: It can be concluded that platelet lysate could be a valuable candidate to replace FBS in cell culture techniques, however, more studies should be done to understand its exact efficacy. UR - https://ijvm.ut.ac.ir/article_67233.html L1 - https://ijvm.ut.ac.ir/article_67233_6b27285e04b89cfea24a32e522178a23.pdf ER -