TY - JOUR ID - 68661 TI - The Effects of L-Citrulline Pretreatment on the Isometric Tension of the Isolated Perfused Rat Aorta JO - Iranian Journal of Veterinary Medicine JA - IJVM LA - en SN - 2251-8894 AU - Hashemi, Seyyed Reza AU - Arab, Hossein Ali AU - Seifi, Behjat AU - Muhammadnejad, Samad AD - Department of Comparative Biosciences, Faculty of Veterinary Medicine AD - Department of Comparative Biological Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran AD - Cell-Based Therapies Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran Y1 - 2018 PY - 2018 VL - 12 IS - 4 SP - 357 EP - 367 KW - Aortic ring KW - Hypertension KW - L-citrulline KW - NO KW - Vasorelaxation DO - 10.22059/ijvm.2018.267039.1004930 N2 - Background: The achievement of an effective compound for prevention/treatment of hypertension with fewer complications has been of interest in recent years. OBJECTIVES: This study aimed to examine the effects of L-citrulline pretreatment on the tension of isolated rat aortic tissues precontracted by different vasoconstrictors. METHODS: Sixteen male Wistar rats (300-350g) were randomly divided into two groups of control and test. The control group was injected 1ml distilled water, while the animal in the test group received 200 mg/Kg L-citrulline (CIT) i.p. for 7days. Rats were euthanized, their thoracic aortas were immediately separated and placed into a petridish containing cold Kerebs-Henseleit solution (KHS). The aorta were cleaned of the surrounding tissues and cut into 4 rings in the presence of 95% O2+5% CO2. The aortic rings divided into 6 subgroups, were suspended into organ bath containing KHS at 37oC. The isolated rings were contracted by 2×10-6 M phenylephrine (Phe) and 60Mm KCl. When the plateau was reached, a cumulative concentration of acetylcholine (Ach) was added into organ bath to induce relaxation. The effects of CIT on relaxation and the role of NO were tested using L-NAME as a pharmacological probe. RESULTS: The pretreatment of rats by CIT significantly (P<0.05) reduced the plateau contraction induced by Phe. CIT also significantly (P<0.01) decreased the contraction induced by KCl and L-NAME+Phe. However, cumulative addition of ACh significantly (P<0.001) decreased the vasoconstriction induced by Phe but not by KCl and L-NAME+Phe in both control and CIT-treated group. CONCLUSIONS: It suggests that, CIT can reduce the rat aorta vasoconstriction through releasing NO. UR - https://ijvm.ut.ac.ir/article_68661.html L1 - https://ijvm.ut.ac.ir/article_68661_870c8539eeeb2d0c7ca18eccad3d3d1d.pdf ER -