TY - JOUR ID - 89862 TI - Phylogenetic Analysis of Attaching and Effacing E. coli Strains Isolated From Pet Birds in Iran JO - Iranian Journal of Veterinary Medicine JA - IJVM LA - en SN - 2251-8894 AU - Abbasi, Mina AU - Peighambari, Seyed Mostafa AU - Razmyar, Jamshid AD - Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Y1 - 2024 PY - 2024 VL - 18 IS - 1 SP - 59 EP - 70 KW - Attaching and effacing E. coli (AEEC) KW - Escherichia coli (E. coli) KW - Phylogrouping KW - Shiga toxin KW - Shiga toxin-producing E. coli (STEC) DO - 10.32598/ijvm.18.1.1005301 N2 - Background: Enteropathogenic E. coli (EPEC) and Shiga toxin-producing E. coli (STEC) are categorized as attaching and effacing E. coli (AEEC) due to their eae gene. One of the essential causes of diarrhea in humans is AEEC, which affects birds, too, thereby being considered a zoonotic pathogen.Objectives: Our study aimed to determine AEEC and evaluate its antibiotic resistance and phylogroups. Methods: A total of 200 fecal samples were collected from pet birds referred to the Veterinary Medicine Hospital, University of Tehran. PCR methods were used to detect AEEC using uspA, eae, bfpA, stx1, and stx2 gene-specific primers. The antimicrobial susceptibility of the recovered isolates was determined by the agar disk diffusion and MIC methods. Their phylogroups were analyzed based on Clermont phylotyping methods.Results: Of 200 samples, we isolated 26 (13%) E. coli strains, 9 harbor eae genes. None of the ease-positive samples possessed the bfpA gene, but 4 had stx2, and 5 had stx1 and stx2 genes. Phylogenetic analysis identified the phylogenetic groups of all AEEC isolated strains but 2 (duck and cockatiel). Detected phylogroups include four B2 and three D. Based on our results, 7 out of 9 AEEC isolated strains showed multi-drug resistance.Conclusion: The discovery of common phylogroups of AEEC in pet birds (a common companion animal in Iran with intimate contact with their owners, especially children) and humans, as well as their resistance to a wide range of antibiotics used in human medicine, verifies AEEC as a serious public health threat. UR - https://ijvm.ut.ac.ir/article_89862.html L1 - https://ijvm.ut.ac.ir/article_89862_4ce482d98d4caba7ae0dc42e0a7c3f7b.pdf ER -