2024-03-29T12:32:51Z
https://ijvm.ut.ac.ir/?_action=export&rf=summon&issue=4325
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Distribution of virulence associated genes in isolated
Escherichia coli from avian colibacillosis
Khatereh
Kafshdouzan
Taghi
Zahraei Salehi
Bahar
Nayeri
Omid
Madadgar
Shinji
Yamasaki
Atsushi
Hinenoya
Nouritomo
Yasuda
BACKGROUND: Colibacillosis is one of the most prevalentdiseases in the world that causes multimillion-dollar annuallosses. OBJECTIVES: In order to evaluate molecular epidemiologyof some virulence associated factors in Escherichia coli,isolated from poultry, the presence of iut A, iss, hly F, omp T, iroN, afa, sfa (S)and pap G (II) were investigated by multiplex PCRassay. METHODS: Two hundred thirty four Escherichia coliisolated from avian colibacillosis (APEC) and fifty four fecal E.coli isolates from the feces of apparently healthy birds (AFEC)were investigated for presence of some virulence associatedgenes by two panel of multiplex PCR. Statistical analysis wasperformed using |c2 test. the p-value was |£|0.05. RESULTS:Among 234 E. coli strains associated with colibacillosis and 54AFEC strains, 85% of isolates were positive for at least one of thevirulence gene. The three most prevalent genes in E. coli isolatedfrom colibacillosis were hly F (77.3%), omp T(73%) and iss(68.2%). Iut A, iro Nand pap G (II) were detected in 157 (67.4%),152 (65.2%) and 41(17.6%) respectively. None of isolatesharbored sfa (s) and afa genes. Several combination patterns ofvirulence genes were detected. Combination of hly F, omp T(70.8%) was the most prevalent pattern. CONCLUSIONS: theprevalence of iss, hly F, omp T, iro N genes in APEC isolates wassignificantly more than AFEC strains and probably these genesplay an important role in the pathogenesis of APEC strains.
Avian pathogenic Escherichia coli
multiplex PCR
virulent factors
2013
04
01
1
6
https://ijvm.ut.ac.ir/article_32017_8437796cc482a67e5069d1e45c2af869.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Effects of mesenchymal stem cells with injectable scaffold on
cardiac function in myocardial infarction in Rabbit
Nazanin
Jafari
Mohammad Mahdi
Dehghan
Mohammad
Abarkar
Mohammad
Hejazi
Pegah
Abbasnia
Mohammad
Molazem
Amir
Tavakoli
Rouh-allah
Mehdinavaz Aghdam
Seyed Hosein
Ahmadi Tafti
Parviz
Tajik
BACKGROUND: Bone marrow-derived mesenchymal cellscan transdifferentiate into Cardiomyocyte cells and improveheart function after transplantation. Since biomaterials canimprove the cell retention in the site, cell survival and differentiation,heart tissue engineering is now being explored as anapplied solution to support cell-based therapies and increasetheir efficacy for myocardial diseases. Chitosan in combinationwith Glycerol Phosphate (GP) can produce a thermo sensitivematerial that in body temperature can form a jellylike material.OBJECTIVES:The aim of this study was to evaluate the effects ofa combination of autologous undifferentiated bone marrowmesenchymal stem cells (MSCs) and injectable scaffold on cardiacfunction improvement in rabbits after inducing myocardialinfarction. METHODS: The Left Anterior Descending (LAD)coronary artery was ligated by No. 6-0 polyamide suturematerial, and autologous MSCs with injectable scaffold wereinjected into the margins of the infarcted zone at the time ofsurgery. At 4 weeks after transplantation, the cardiac functionand structure was detected using echocardiography. RESULTS:There was no significant difference among the three groups (MIonly, MI Scaffold, and MI+Scaffold+MSCs) in the Echocardiographicparameters including, heart rate (HR), Ejection Fraction(EF), Fractional Shortening (FS), Left Ventricular Diameter(LVD) and Left Ventricular Parietal Wall Diameter (LVPW).CONCLUSIONS: A combination of autologous undifferentiatedbone marrow MSCs and injectable scaffold made of Chitosan+Glycerol Phosphate in echocardiographic evaluation did nothave a positive influence on achieving functional improvement.
Echocardiography
mesenchymal
stem cell
Scaffold
ejection fraction
fractional shortening
2013
04
01
7
13
https://ijvm.ut.ac.ir/article_32018_79e1ddedcc421480fe1cdee3769a13ce.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
The mRNAexpression study on small amount of Theileria
annulata lymph node biopsy sample using SMART-cDNA
technology
Nastaran
Sadr Shirazi
Parviz
Shayan
Berigiteh
Eckert
Elaheh
Ebrahimzadeh
Sedigheh
Jafari
BACKGROUND: A major issue in many gene expressionstudies utilizing small amount of biological materials is thelimited quantity of RNApurified from clinical samples, which isoften used for RT-PCR or standard Northern blot analysis.OBJECTIVES: The SMART cDNA synthesis method and subsequentSMART-cDNA-PCR technique was used to analyse 3genes in macroschizonts of Theileria annulata in small lymphnode biopsy material. METHODS: The SMART-cDNA of TaSpgene was cloned in pTZ57R/T-vector and sequenced. We focusedon genes encoding surface proteins TaSp, TaD and HSP70.RESULTS: Our results showed that SMART cDNA dependablyreproduces the expression profile found in messenger RNA. TheRT-SMART-PCR showed the amplification of the processedmRNAs. The sequencing analysis showed that the amplifiedcDNA was coded for TaSp protein in Theileria annulata.CONCLUSIONS: It was concluded that the SMART PCRtechnique is practical for amplification of complete sequence ofmRNAs in the form of cDNAs, and therefore for gene expressionstudies if only small amounts of starting material are available.
Theileria annulata
SMART-cDNA
TaSp
TaD
HSP70
2013
04
01
15
22
https://ijvm.ut.ac.ir/article_32019_3e488d7a07aad80a635656ada23e1571.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Molecular characterization of non-structural gene of H9N2
subtype of avian influenza viruses isolated from broiler
chickens in Iran
Hesam-aldin
Emadi Chashmi
Mahdi
Vasfi Marandi
Mohammad Hasan
Bozorgmehrifard
Mohsen
Bashashati
Abbas
Barin
BACKGROUND: The H9N2 subtype of avian influenzaviruses (AIVs) have spread in Asia and Middle East countriesand have become a serious threat to poultry industry in Iran.OBJECTIVES:Characterization of genes of H9N2 subtype involvingin pathogenicity and diagnosis are crucial in control of avianinfluenza outbreaks. The Nonstructural (NS) gene and its proteinproducts (NS1 & NS2) are important as diagnostic marker, lifecycle and pathogenicity of AIVs. METHODS:The NS gene of fivestrains, isolated from 1998 to 2010, were completely sequencedand analyzed. RESULTS:All of the examined strains were composedof 890 nucleotides with 230 amino acids. In this regard, onlytwo Iranian strains from GeneBank had 217 amino acids in NS1protein. All Iranian H9N2 strains subdivided into two distinctsublineages including I and II. Comparative analysis of NS genesof Iranian strains showed that since 2003, they might haveoriginated from Pakistan H7N3 strains; whereas from 2008 theycould be originated from Pakistan H9N2 strains. CONCLUSIONS:Although the low pathogenic H9N2 subtype has been permanentlycirculating from 1998 to the present in Iran, phylogeneticanalysis of NS genes revealed that sublineage II has circulatedmore in poultry industry of Iran. These epidemio-logicallyvariations could be related to vaccination pressure due tomassive vaccination or NS gene reassortment in rural andbackyard chickens.
Avian influenza virus
H9N2 subtype
nonstructural gene
Poultry
2013
04
01
23
34
https://ijvm.ut.ac.ir/article_32020_300b5d47321ba06e9c430a8ef76de3e7.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Characterization of Salmonella isolates from poultry
sources in Iran
Seyed Mostafa
Peighambari
Ramin
Akbarian
Rima
Morshed
Azam
Yazdani
BACKGROUND: Salmonellosis is one of the most importantzoonotic diseases throughout the world. OBJECTIVES: Thepurpose of this study was to characterize a large collection ofSalmonella isolates from different poultry sources in Iran.METHODS: A total of 123 Salmonella isolates from differentpoultry sources were subjected to drug susceptibility test,hemolysin production, motility test, and plasmid profile (50isolates). RESULTS: Seventy-one resistance patterns were foundto 29 antimicrobial agents among 123 Salmonella isolates, inwhich 81% of isolates were resistant to more than oneantibacterial agent. The resistance patterns of 123 isolates to 10commonly used antibacterials in Iranian poultry industry werealso quite variable and included 31 patterns. Four differentplasmid patterns were found among 50 Salmonellaisolates. Fiftyfour percent of Salmonella isolates harbored one or threeplasmids with approximate molecular size ranging from 2.3 to68 kb. No plasmid was detected in 46% of isolates. Aband of 68kb size was detected in all isolates that harbored plasmid. Allisolates were motile but no isolate showed hemolysinproduction. CONCLUSIONS: The frequency of resistance toantibacterial agents among avian Salmonella isolates is a majorpublic health concern.
Salmonella
drug susceptibility
plasmid profile
Poultry
2013
04
01
35
41
https://ijvm.ut.ac.ir/article_32021_1c61cc2f7276f0b6dadc33d7e3a4f269.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Seroepidemiology and molecular detection of Brucella infection
in Iranian horses: Aprovincial study
Khalil
Badiei
Hasan
Sharifiyazdi
Mehrdad
Pourjafar
Mohsen
Ghane
Seyyed Adol-Nabi
Hashemi
BACKGROUND: Brucellosis is a febrile zoonotic infectionand has worldwide distribution among humans as well asanimals. Although the seroprevalence of brucellosis in variousanimals has been described in Iran, there is only one report onequine brucellosis in the region. OBJECTIVES: This study wascarried out to determine the seroprevalence of brucellosis inracing clubs and private horse owners in the south of Iran and riskfactors associated with the disease in horses. METHODS: 312randomly selected equine serum samples were investigated forthe presence of antibodies against Brucella genus, using slideagglutination by Rose Bengal plate test (RBPT), serum agglutinationtest (SAT) and 2-mercaptoethanol (2-ME) test, usingwhole cell antigen. PCR assay was also used for detection ofclinically suspected cases. RESULTS: Most seropositive horsesin this study were asymptomatic. The true seroprevalence ofbrucellosis was found to be 9.9, 8 and 7% by RBPT, SAT and 2-mercaptoethanol tests, respectively. All horses with history ofclinical signs (3.2% of all samples) had RBPT, SAT and 2-mercaptoethanol positive results. It was also revealed that age,sex and a history of contact with ruminants had no effect onacquiring the infection in positive cases. In the PCR, one of thethree horses with fistula withers produced amplicon of 450 bpfragment of wbo sequences specific to Brucella spp. field strain.CONCLUSIONS: This study showed the seroprevalence ofbrucellosis in horses of Fars province and it was indicated thatthe PCR assay may be helpful in detection of clinically suspectedhorses.
Horse
Brucella infection
PCR
2013
04
01
43
49
https://ijvm.ut.ac.ir/article_32022_94ce46da136eceef48aa41cbb54a299e.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
An outbreak of abortion in Afshari sheep with probable
involvement of Campylobacter fetus
Masoumeh
Saleh
Mohammad Taher
Harkinezhad
Alireza
Marefat
Vahid
Salmani
BACKGROUND:Abortion is one of the most important factorsreducing lambing rate and consequently profitability of sheepfarms. In addition to financial losses, it is also important from azoonotic point of view. OBJECTIVES: The aim of this study wasto investigate bacterial abortifacient agents in an outbreak ofabortion occurring in Afshari sheep in the northwest of Zanjanprovince. METHODS:Vaginal swab samples were collected from217 Afshari ewes (129 samples were taken from aborted ewes, 3samples from ewes with crippled and deformed lambs, and 85samples from animals that had given birth to healthy lambs) fromreported flocks involved in outbreak. Swabs were examined byPCR assay to detect DNAfrom Coxiella burnetii, Chlamydophilaabortus, Salmonella enterica, Yersinia enterocolitica, Campylobacterfetus, Brucella ovis and Leptospira interrogans. RESULTS:Based on the results, only DNA of Campylobacter was detectedin the samples. A 266 bp fragment specific for Campylobacterwas amplified from 51.52% and 34.12% samples belonging toaborted and non-aborted ewes, respectively. CONCLUSIONS:Significant presence of the bacterium in aborted ewes (p<0.001)compared to the non-aborted groups with odd ratio of 3,emphasizes that Campylobacter could be involved in theoutbreak of the abortion. Considering the importance of thedisease, prophylactic measures are needed to reduce the disease.However, further investigations are required to determine theimpact of this bacterium in prevalence of abortion in sheep inother areas.
PCR
Campylobacter
abortion
afshari ewes
2013
04
01
51
56
https://ijvm.ut.ac.ir/article_32023_fb8844e18687f293377ccc49056ff4c5.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Comparison of immunochromatographic rapid test with
molecular method in diagnosis of canine parvovirus
Shahab-aldin
Mohyedini
Shahram
Jamshidi
Sima
Rafati
Gholam Reza
Nikbakht Boroujeni
Abdolali
Malmasi
Yasaman
Taslimi
Hesam-aldin
Akbarein
BACKGROUND: Canine parvovirus (CPV) infection is one ofthe most common causes of infectious gastroenteritis in dogs andis a highly contagious, often fatal disease. The original virus(CPV type 2) has had some mutations since its emergence andnew variants (CPV-2a, 2b and 2c) have been reported from manycountries all around the world. Early diagnosis and treatment canprofoundly affect the disease outcome. OBJECTIVES:To comparethe ability of Immunochromatographic (IC) test to detect CPVinfection in 50 PCR positive samples (n=50) with regard to virusstrains. METHODS: 50 rectal swabs (n=50) were prepared fromsuspicious dogs and subjected to PCR and IC test respectively.RESULTS: The sensitivity of IC test in PCR positive samples was84% (42 out of 50 samples) and the positive predictive value ofthe test was 100%. Using PCR, CPV strains in our study were 2a(18/50, 36%) and 2b (32/50, 64%) with the predominance of 2bstrain. IC test was also able to diagnose 15/18 (83.3%) of CPV-2a and 27/32 (84.3%) CPV-2b strain positive samples, whichmeans IC test can detect CPV infections caused by both virusstrains (2a and 2b), without significant difference. CONCLUSIONS:This study shows that IC test results are relatively reliable fordiagnosing CPVinfection in daily veterinary practice and the testis able to diagnose both CPV-2a and CPV-2b which are prevalentstrains in Iran.
canine parvovirus
detection methods
PCR
immunochromatography
2013
04
01
57
61
https://ijvm.ut.ac.ir/article_32024_674aafe016c520e2e20d036ec268ac53.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Histological study of cartilaginous cells in the body of penis in
one-humped camel (camelus dromedarius)
Mohammad Hasan
Yousefi
Hasan
Gilanpour
Mohammad Reza
Salimi-Bejestani
BACKGROUND:Several investigations showed cartilaginouscells in fibrous tissue of the free part of the penis in one humpedcamel. OBJECTIVES: The aim of this study was accurate assessmentof existence of cartilaginous cells in penis shaft of onehumpedcamel. METHODS: Six camel penises from maturedcamels more than 3 years-old were collected from an abattoir.Different specimens were prepared from each penis and kept in10% formalin container for fixation. After passing differentstages of histotechnique methods, several slides were preparedfrom each specimen, stained with Haematoxylin Eosin andstudied. RESULTS: Results showed that the majority of cartilaginouscells were inside the collagen fibers of tunica albuginea andaround corpus cavernosum and corpus spongiosum of penis andtheir distributions were dissimilar in different parts of the penisshaft. This survey further showed that in penis shaft length, themajority of cartilaginous cells were inside tunica albuginea,which is surrounded by corpus spongiosum and particularly, theventral surface of urethra. CONCLUSIONS: The number ofcartilaginous cells decreased gradually from distal extremitytowards the proximal extremity of the body of the penis andincreased gradually from external layer of tunica albugineatowards the internal layer of tunica albuginea and centre ofcorpus cavernosum penis. Existence of cartilaginous cells insidethe leaf tissue of the penis was seen with aging and puberty.
Camel
Histology
penis
Cartilage
2013
04
01
63
67
https://ijvm.ut.ac.ir/article_32025_31835449d44e4a1765f47b52436a628e.pdf
Iranian Journal of Veterinary Medicine
Iran J Vet Med
2251-8894
2251-8894
2013
7
1
Probable occurrence of black leg in a sucker dairy calf: the
necessity of providing sufficient maternal antibody in endemic
regions
Javad
Tajik
Aziz Allah
Khodakaram Tafti
Abdollah
Derakhshandeh
Tahmineh
Tajik
Black leg has been reported in a variety of animals, but is ofthe most importance in cattle and sheep. A20 days old Holsteindairy calf was examined because of anorexia and lameness from2 days ago. The calf was depressed, tachypneic, tachycardic, andhad a body temperature of 38.5ºC. Both hind limbs proximal tothe tarsal joint were markedly swollen, firm and painful. Nocrepitation was noted on palpation. The calf had bruxism, stiffnessof gait and unwillingness to move. At necropsy, massivenecrosis of thigh muscles which caused dark discolorated tissuewith metallic sheen, large amount of thin sanguineous exuda andabundant gas bubbles were evident in the underlying tissues.Histopathologic examination revealed extensive degenerationand coagulative necrosis of muscle fibers and supported adiagnosis of black leg. No vaccination against Clostridiumchauvoei was applied in the herd and the calf did not receivenotable maternal antibody. Providing sufficient maternalantibody or early vaccination of the susceptible newborn calvesshould be considered in the endemic regions.
black leg
sucker calf
Dairy
maternal antibody
endemic regions
2013
04
01
69
72
https://ijvm.ut.ac.ir/article_32026_18bab4326d2417c4f9e602a06f007552.pdf