University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 20 2 2026 03 01 Seroprevalence of Antibodies of Crimean-Congo Hemorrhagic Fever Virus in Cattle in the Turkestan Region of Kazakhstan 271 278 106048 10.32598/ijvm.20.2.1005804 EN Balzhan Myrzakhmetova Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Lespek Kutumbetov Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Talshyngul Tlenchiyeva Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Aiganym Tussipova Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. 0000-0002-7767-0542 Gulzhan Zhapparova Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Karina Bissenbayeva Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Kuandyk Zhugunissov Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Sergazy Nurabayev Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Aslan Kerimbayev Research Institute for Biological Safety Problems LLP, QazBioPharm National Holding, Gvardeyskiy, Kazakhstan. Journal Article 2025 05 13 <strong>Background</strong>: Crimean-Congo hemorrhagic fever (CCHF) is endemic in southern Kazakhstan, yet data on livestock exposure remain scarce. Because cattle can amplify virus-infected ticks, accurate prevalence estimates are essential for risk-based control.<br />Objectives: This study aimed to determine the seroprevalence of CCHF virus (CCHFV) antibodies in cattle across the Turkestan region and to identify demographic and spatial factors associated with exposure.<br /><strong>Methods</strong>: In 2024, a cross-sectional survey generated 840 bovine serum samples (420 in spring and 420 in autumn) from 14 districts of the Turkestan Region, Kazakhstan. Sampling was performed using a random approach with stratification: 42 rural settlements were included (3 rural districts per district, and 10 heads of cattle from each district). Animals were selected from multiple owners in each village, ensuring the data were representative for assessing the cattle population in the region. CCHFV antibodies were detected using a commercial double-antigen enzyme-linked immunosorbent assay (ELISA), which enhances specificity and reduces the risk of cross-reactivity with related viruses. Spatial variation in seroprevalence was visualized in ArcGIS 10.8, while statistical analysis, comprising descriptive summaries, χ² test, and multivariable logistic regression, was conducted in R software, version 4.1.0. Odds ratios (OR) with 95% confidence intervals (CI) were calculated. Results with P=0.05 were deemed significant.<br /><strong>Results</strong>: The overall seroprevalence was 36.2%, with a significant range of district-level seroprevalence from 10% to 76.6%. The study identified higher seropositivity in adult cattle (43.42%) than in juveniles (11.86%), and females had slightly higher rates than males (38.32% vs 29.99%). Logistic regression analysis showed that age was a significant risk factor, with adults having 4.06 times higher odds of seropositivity (OR=4.06; 95% CI, 2.62%, 6.29%) compared to juveniles. <br /><strong>Conclusion</strong>: The findings confirm active, heterogeneous circulation of CCHFV among cattle in the Turkestan region, with older animals and districts practicing communal grazing at greatest risk. Incorporating livestock serosurveillance into routine tick control programs and focusing interventions on identified hotspots could curtail zoonotic spill-over to humans.  https://ijvm.ut.ac.ir/article_106048_056f73b27babb8c6d01c3c80e8c0943e.pdf