University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 3 1 2009 07 01 Laboratory evaluation of three strains of the entomopathogenic fungus Metarhizium anisopliae for controlling Hyalomma anatolicum anatolicum and Haemaphysalis punctata استفاده از قارچ متاریزیوم آنیزوپلیه در کنترل بیولوژیکی هیالوما آناتولیکوم آناتولیکوم و همافیزالیس پونکتاتا در شرایط آزمایشگاهی ‌ ‌ 19604 10.22059/ijvm.2009.19604 EN Mosa Tavassoli دانشکده دامپزشکی Abdol Ghaffar Ownag دانشکده دامپزشکی RuGhayeh Meamari بخش خصوصی Salaheddin Rahmani بخش خصوصی Karim Mardani دانشکده دامپزشکی Taregh Butt Journal Article 1970 01 01 Hyalomma anatolicum anatolicum and Haemaphysalis punctata larval ticks were shown to be susceptible to different strains of entomopathogenic fungi Metarhizium anisopliae under laboratory conditions. To determine the susceptibility of H. anatolicum anatolicum and H. punctata to M. anisopliae, two suspensions of conidia were used (103 and 104 spores/ml). The treatments were conducted by immersing larval stage (at least 20 larvae/Petri dish) of H.anatolicum anatolicum and H.punctata in the spore suspension for 30 sec, followed by transferring to Petri dish containing moist filter paper. Control larval ticks were only immersed in 0.05% aqueous Tween 80. All treated and untreated ticks were observed by day interval up to day 18 to detect dead ticks and signs of mycosis. Comparative results of bioassays using three different fungal strains showed that all strains of M. anisopliae were highly pathogenic against two tick species used. The higher mortality rate was seen with strain of 689 when used in H. punctata and 685 in H. anatolicum anatolicum in comparison with the other strains(p<0.05). The present study suggests that Metarhizium anisopliae has great potential for the control of H. punctata and H. anatolicum anatolicum. https://ijvm.ut.ac.ir/article_19604_46300280f3bb037325427bd21788dd91.pdf