University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 3 1 2009 07 01 Growth and toxigenesis behavior of Clostridium botulinum type E in Persian sturgeon (Acipenser persicus) Caviar prepared with various preservatives رفتار رشد توکسین‌زایی کلستریدیوم بوتولینوم تیپ Eدر خاویار تاس‌ماهی ایرانی فرآوری شده با برخی نگهدارنده‌ها‌ 19611 10.22059/ijvm.2009.19611 EN .A Salmani پژوهشکده اکولوژی آبزیان دریای خزر .R Safari دانشکده دامپزشکی .M Soltani داتشکده دامپزشکی دانشگاه تهران .H.R Tavakoli دانشکده بهداشت دانشگاه علوم پزشکی بقیه ا... Journal Article 1970 01 01 Growth behavior of Clostridium botulinum type E beluga was studied in Acipenser persicus granular caviar treated with 5% NaCl, 5%NaCl plus 0.3% boric acid and 0.4%borax and 5%NaCl plus 0.15% methyl paraben incubated at temperatures -2, 5 and 15 °C for 224 days. The initial number of inoculated bacterial spore was 5.1×104 cfu/g caviar in each treated sample. The number of bacterial spores in samples treated with 5% NaCl changed to 3.59 × 104, 1.02×105 and 9.9×105 cfu/g at -2 °C, 5 °C and 15 °C, respectively, while those samples treated with 5%NaCl plus 0.3% boric acid and 0.4%borax changed to 1.56×104, 3.65×104 and 9.22×104 cfu/g, respectively. Also, number of bacterial spores in samples treated with 5% NaCl plus 0.15% methyl paraben changed to 1×104, 2.86×104 and 3.56×104 cfu/g at the above storage temperatures, respectively. Fourteen days post-inoculation, toxin production was positive in samples treated with 5% NaCl incubated at 5 °C and 15 °C, while those samples treated with 5%NaCl plus 0.3% boric acid and 0.4% borax were positive for toxin production only at 15 °C. Toxin production was negative in samples treated with 5%NaCl plus 0.15% methyl paraben incubated at all three above mentioned temperatures. These data showed that 5%NaCl plus methyl paraben had more inhibitory effect than boric, borax and NaCl. Also, such inhibitory effects can be increased at lower storage temperature of -2°C. Therefore, due to restricted usage of boric acid and borax in granular caviar, use of methyl paraben can be recommended as a safe preservative with a strong antimicrobial effect at caviar pH. https://ijvm.ut.ac.ir/article_19611_1885c19d81f1104cecd66bb1e4387a1c.pdf