University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 6 1 2012 03 01 Identification of Gyrodactylus gurleyi in Carassius auratus using morphometric and molecular characterization شناسایی انگل ژیروداکتیلوس گرلیی در ماهی کاراسیوس اوراتوس با استفاده از ‌بررسی مورفومتریک و ملکولی 41 46 24624 10.22059/ijvm.2012.24624 EN حسینعلی ابراهیم زاده موسوی گروه بهداشت و بیماریهای آبزیان، دانشکده دامپزشکی، دانشگاه تهران Hossein Ali Ebrahimzadeh Mousavi گروه بهداشت و بیماریهای آبزیان، دانشکده دامپزشکی، دانشگاه تهران Mahdi Soltani گروه بهداشت و بیماریهای آبزیان، دانشکده دامپزشکی، دانشگاه تهران Parviz Shayan گروه انگل شناسی، دانشکده دامپزشکی، دانشگاه تهران Elaheh Ebrahimzadeh گروه انگل شناسی، دانشکده دامپزشکی، دانشگاه تهران Mohammad Hoseini گروه پاتولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد بابل Journal Article 1970 01 01 BACKGROUNDS: Gyrodactylus is a small monogenean ectoparasite that lives on the skin and fins of most of the world's fish species. Gyrodactylus appears to be one of the most prevalent parasites found in ornamental fish, especially in Cyprinids. Goldfish (Carassius auratus) are a popular ornamental fish that are highly contaminated by Gyrodcatylus. OBJECTIVES: The present study is aimed to identify morphological and molecular character-istics of the Gyrodactylus parasite on gold fish. METHODS: The morphological identification of Gyrodactylus specimens was performed using the measurements and drawings of opisthaptoral hard parts of the parasites. The molecular species description was based on a polymerase chain reaction (PCR) of partial sequence of the 5.8S region of ribosomal RNA, and a partial sequence of the internal transcribed spacer 2 (ITS2) of ribosomal RNA. The nucleotide sequences of the PCR products were compared with corresponding sequencing registered in GenBank. RESULTS: Based on the morphometric analysis and sequencing, the Gyrodactylus specimens were described as Gyrodactylus gurleyi. CONCLUSIONS: A combination of molecular techniques with morphological analysis seems to be the best approach for the identification of Gyrodactylus speices. https://ijvm.ut.ac.ir/article_24624_dbe887405f6b74c6a5bc35a633cf9ddf.pdf