University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 7 2 2013 07 01 Preventive effect of omega-3 fatty acids on ethinyl estradiolinduced hepatosteatosis in female wistar rat اثر محافظتی اسیدهای چرب امگا–3 برهپاتو استئاتوز القاء شده توسط اتینیل استرادیول در رتهای ماده 129 134 35061 10.22059/ijvm.2013.35061 EN Marjan Chahardahcherik Department of Basic Sciences, Faculty of Veterinary Medicine Shahid Chamran University, Ahwaz, Iran Ali Shahriari Department of Basic Sciences, Faculty of Veterinary Medicine Shahid Chamran University, Ahwaz, Iran Peyman Asadian Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran Saleh Esmaeilzadeh Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahwaz. Iran. Journal Article 2013 02 06 BACKGROUND: Hepatic steatosis due to estrogen therapy<br />increases the activity of inflammatory markers, particularly the<br />activity of TNFα which in turn induces more lipogenesis.<br />Omega-3 fatty acids are among the negative regulators of hepatic<br />lipogenesis. OBJECTIVES: In this research, the preventive effect<br />of omega-3 fatty acids on estrogen-induced steatosis in rats was<br />evaluated. METHODS:2 mg/kg. BW/SC of 17α-ethiny-lestrasdiol<br />were injected into 25 female wistar rats in 5 equal groups<br />(excluding the control group) over 10 consecutive days.<br />Simultaneously, 3 of estradiol-treated groups were orally given<br />250, 500, and 1000 mg/kg. BW omega-3 fatty acids, respectively.<br />At the end of the experiment, plasma ALT, AST, and TNFα<br />level were determined. Histopathological changes in the liver<br />were also identified by the evaluation of samples stained with<br />H&E and Oil Red O. RESULTS: The histological findings<br />revealed hepatic microvesicular steatosis and fat deposit in<br />ethinylestradiol and, to a lesser extent, in the 250 mg/kg BW<br />omega-3 fatty acids groups. The plasma levels of AST, ALT, and<br />TNFα significantly increased in the ethinylestradiol group<br />compared to the control (p<0.05) and 1000 mg/kg. B.W omega-<br />3 group. Omega-3 fatty acids reduced these parameters in<br />comparison to the estradiol group (p<0.05). CONCLUSIONS: It<br />was concluded that 1000mg/kg.BW of omega-3 protects the<br />liver against steatotic injuries. https://ijvm.ut.ac.ir/article_35061_ffb3d5ed7a56a8a9e43ae22e7944fc5b.pdf