University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 7 3 2013 10 01 Serological and bacteriological study of leptospirosis in dairy herds and feedlot in Tehran suburbs مطالعه سروباکتریولوژیک لپتوسپیروز در گاوداری های شیری و پرواری اطراف تهران 177 183 35968 10.22059/ijvm.2013.35968 EN Shahram Maleki Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Gholamreza Abdollahpour Department of Hygiene and Food control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Alireza Bahonar Department of Hygiene and Food control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2013 03 04 BACKGROUND: Leptospirosis is a worldwide zoonosis caused<br />by Leptospira interrogans. Leptospirosis results in decreased<br />milk production, abortion, stillbirth, infertility and mortality,<br />which causes financial loss in the cattle industry. OBJECTIVES:<br />The aim of this research was to perform a serological and<br />bacteriological study of leptospirosis in 6 industrial dairy herds<br />and 3 feedlots with previous records of leptospirosis in Tehran<br />suburbs in 2011-2012. METHODS: For the purpose of this study,<br />408 blood samples from dairy cattle and 154 blood samples from<br />feedlots were collected using sterile 10ml venoject vacutainers<br />from tail vein. Two months later, 118 urine samples were<br />collected from 20% of the two groups of serological negative and<br />positive animals. All serum samples were serologically tested by<br />microscopic agglutination test (MAT), a standard method for<br />serological diagnosis of leptospirosis. The serum samples were<br />tested for antibodies against five live antigens of Leptospira<br />interrogans serovars: Pomona, Grippotyphosa, Hardjo, Icterohaemorrhagiae<br />and Canicola. Urine samples were used for<br />bacteriological isolation of Leptospira spp. RESULTS: Serological<br />results showed that 228 (40.6%) of animals had a positive<br />reaction against one or more serovars. The most prevalent<br />Leptospira serovars was Pomona 118 (40.3%) and the least<br />prevalent was Canicola 4 (1.4%). The most prevalent titer was<br />1:100, and the highest titer was 1:1600. Also the most<br />seropositive cases were observed in 3 to 4-year-old cows.<br />Bacteriological results revealed that in 11 (9.3%) urine samples<br />Leptospira spp. were isolated, all taken from one feedlot farm.<br />According to the history taken from each farm, the main risk<br />factors were the presence of rodents and low hygienic conditions<br />of the farms. CONCLUSIONS: The results of this study revealed<br />that cows could have a major role in maintaining Pomona,<br />Grippotyphosa and Hardjo serovars; indeed, they are a potential<br />zoonotic risk to slaughter house workers, meat inspectors,<br />milkers and farmers. https://ijvm.ut.ac.ir/article_35968_ce805ddb61a2a853949ebbc5f4e7f6cc.pdf