University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 7 3 2013 10 01 Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011 تشخیص کوکسیلا برونتی با استفاده از آزمون زنجیره‌ای پلی مراز آشیانه‌ای در شیرهای فله‌ای گاودرای های استان قم در سال 1390 207 211 35972 10.22059/ijvm.2013.35972 EN Arash Ghalyanchi Langeroudi Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Narges Babkhani Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Mohammad Reza Zolfaghari Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Keivan Majidzadeh Arbadili TASNIM Biotechnology Research Center, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran Abbas Morovvati Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Mohammad Soleimani Department of Microbiology, Qom Branch ,Islamic Azad University, Qom, Iran Journal Article 2013 01 09 BACKGROUND: Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed worldwide. In cattle, Coxiella burnetii infections are generally asymptomatic but can also be associated with reproductive disorders. OBJECTIVES: The aim of this study was to achieve molecular detection of Coxiella burnetii in dairy bovine milk farms using Nested PCR in Qom province, Iran. METHODS: From January to February 2011 (winter) and July to September 2011(summer) a total of 100 bovine bulk milk samples were equally collected from five areas of Qom. The nested PCR assay used to screen for C. burnetii was designed from the nucleotide sequence of the com1 gene encodin a 27-kD outer membrane protein (OMP). RESULTS: In this study, 14% (14 of 100) of bulk milk were positive. CONCLUSIONS: These results support the hypothesis of high prevalence and endemic pattern of Q fever in Qom province of Iran.

https://ijvm.ut.ac.ir/article_35972_2904fe92563ec35289d7bf19a60d6e3e.pdf