University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 7 3 2013 10 01 Blood and tissue levels of diazinon in rabbit following a subacute dermal exposure to incremental doses تعیین میزان دیازینون در سرم و بافت‌های خرگوش بدنبال تماس تحت بالینی دوزهای افزایشی از راه پوست 213 219 35973 10.22059/ijvm.2013.35973 EN Hoseinali Arab Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Masoud Goudarzi Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Mohammadkazem Koohi Departemt of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Gholamreza Shams Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2013 03 10 BACKGROUND:Uncontrolled application of diazinon (DZN)<br />can cause environmental contamination and adverse health<br />effects on humans or animals. OBJECTIVES:This study aimed to<br />investigate the toxic effects and the level of DZN in serum and<br />tissues of rabbits following a sub acute dermal exposure to<br />toxicant. METHODS:Different doses of DZN were applied daily<br />to New Zealand rabbits through the ear skin in incremental doses<br />for 4 weeks. Blood samples were collected at the beginning and<br />the end of each dose-week period. Tissue samples were collected<br />from brain, muscle, kidney and liver on day 28, after euthanizing<br />the rabbits. DZN contents of the blood and tissue samples were<br />measured using a reversed phase HPLC system. RESULTS:<br />Clinical observations indicated signs of toxicity in the animals<br />exposed to DZN as shown by diarrhea and body weight loss from<br />day twenty. The level of DZN in the blood elevated with<br />enhancing exposure time and reached the highest level at the end<br />of the fourth week (0.620±0.26ppm). The highest level of DZN<br />was found in the brain tissue (0341±0.015 ppm). CONCLUSIONS:<br />The results of this study revealed the tissue accumulation and<br />subsequent toxic effects of DZN following the subacute dermal<br />exposure to the toxicant. It suggests that the determination of the<br />toxicant level in the serum or tissue can be a monitoring method<br />for the detection of the contamination rate. https://ijvm.ut.ac.ir/article_35973_a776751fbba28300348c1a88efd2ab20.pdf