University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 14 2 2020 04 01 The in vitro Effect of Doxorubicine-G2-FA Treatment on Breast Cancer Copyright تاثیر in vitroدوکسوروبیسین FA-G2- بر سرطان سینه 147 158 76816 10.22059/ijvm.2019.291998.1005039 EN Hamed Mansoor Lakooraj 1Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Zohreh Khaki Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 0000-0003-2267-2185 Masoud Ghorbani Pasteur Institute of Iran, Research and Production Complex, Department of Research and Development Mehdi Shafiee Ardestani 3Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Omid Dezfoulian 4Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran Journal Article 2019 11 09 BACKGROUND:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">To achieve delivery of a drug to tumors, folic acid (FA) was used as a targeting ligand to</span></span><br /> change nanocarriers. Since the folate receptor has more expression in several tumor types such as breast cancer.<br /> OBJECTIVES:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">The present study aimed to evaluate the effect of Doxorubicine-G2-FA (Dox- G2-FA) with</span></span><br /> in vitro assays. The abbreviation of G2 represents the second generation of dendrimer synthesis.<br /> METHODS:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">For this purpose, Dox-G2-FA was synthesized and mass spectroscopy was used to confirm the</span></span><br /> synthesized component. Also, MTT assay, flow cytometry, and gene expression assay by real-time PCR were<br /> used to evaluate cell viability, apoptosis, and necrosis.<br /> RESULTS:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">In this study, the effect of Dox and Dox-G2-FA on the expression of Bax, Bcl2 genes showed</span></span><br /> that there was a significant decrease in the expression of the Bcl2 gene in the Dox-G2-FA group compared to<br /> Dox and control groups(<br />  <br /> <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;">P</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><0.05). Also, the results of flow-cytometry showed that apoptosis in the presence of</span></span><br /> Dox-G2-FA was greater than in the Dox group (<br />  <br /> <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;">P</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><0.05).</span></span><br /> CONCLUSIONS:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">Therefore, it seems that the effect of Dox-G2-FA on apoptosis is better than the effect of</span></span><br /> Dox usage alone. https://ijvm.ut.ac.ir/article_76816_00d9a550c05698bf3c0d4d8faed788ee.pdf