University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 16 4 2022 10 01 Effects of Quebracho Tannin Extract on Testicular Ischemia-/Reperfusion اثرات تجویز عصاره تانن کبراکو بر آسیب متعاقب ایسکمی/رپرفیوژن بیضه در موش صحرایی 423 431 85860 10.22059/ijvm.2022.333335.1005206 EN Zahra Ghotbitabar Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Ahmad Asghari Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran 0000-0001-5152-8807 Shahin Hassanpour Department of Physiology Faculty of Veterinary Medicine Science and Research Branch Islamic Azad University-Tehran-Iran Alireza Jahandide Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Journal Article 2021 11 02 BACKGROUND: Ischemia/reperfusion (I/R) injury is one of the main pathophysiologic conditions of the testis. Although free radical scavenging properties of the tannins are approved, limited information exists on their antiox-idant activities. OBJECTIVES: The aim of this study was to evaluate the effect of quebracho tannin extract (QTE) on testicular I/R injury in rats. METOD: Thirty male Wistar rats were randomly divided into 5 experimental groups (n=6). One group was kept as control. In I/R group, rats were subjected to 4 hours I/20 hours R, and the group was intraperitoneally (ip) injected with saline 1 hour before R. In groups 3-5, rats were subjected to 4 hours I/20 hours R, and ip injected with QTE (100, 200, and 400 mg/kg) 1 hour before R. At the end of the study, left testis was removed for histological analysis and antioxidant measurement. RESULTS: I/R resulted in degenerated seminiferous tubules and loss of spermatogenesis. Administration of QTE (200 and 400 mg/kg) to I/R rats showed many normal seminiferous tubules. Tissue malondialdehyde (MDA) levels significantly increased in I/R rats (P<0.05) while QTE decreased IR-induced MDA level (P<0.05). Experimental I/R significantly decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities (P<0.05). Ad-ministration of QTE significantly increased tissue SOD and GPx activities in I/R rats (P<0.05). CONCLUSIONS: These findings suggested treatment with QTE had a benefial effect against I/R.

https://ijvm.ut.ac.ir/article_85860_7d1103ee87775fb8022498553cb650e7.pdf