University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 18 4 2024 10 01 Pichia pastoris an Ideal Host for the Production of Recombinant Influenza Vaccines 465 478 97192 10.32598/ijvm.18.4.1005523 EN Amir Asghari Baghkheirati Department of Avian Health and Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Mohammad Hadi Sekhavati Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Seyed Mostafa Peighambari Department of Avian Health and Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Kiarash Ghazvini Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Jamshid Razmyar Department of Avian Health and Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Journal Article 2024 02 17 Pichia pastoris is a methylotrophic yeast with remarkable characteristics such as lacking endotoxin, producing high amounts of recombinant protein, performing post-translational modifications, and so on. Influenza A virus, a member of the Orthomyxoviridae family, is the cause of avian influenza. Three avian influenza virus subtypes, H5, H7 and H9, are commercially and physiologically significant in the poultry industry. Some researchers considered influenza to be the next pandemic disease. Nowadays, researchers have paid attention to producing novel and effective recombinant vaccines, especially in the poultry industry. Due to the advantages of P. pastoris yeast, it can be used as an ideal expression system for producing subunit vaccines. Although several studies have been conducted in this field, there is no comprehensive review of using P. pastoris to produce recombinant influenza vaccines. This review explains the different strains, phenotypes, and advantages of this yeast and then the production of recombinant influenza vaccines using this expression system is discussed in detail. https://ijvm.ut.ac.ir/article_97192_3316b12d7302d183457b19144394911a.pdf