University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Influence of Graded Dose of Moringa oleifera Seed Extract Administered Orally on Testicular Pathology, Gonadal and Extra Gonadal Sperm Reserves of Wistar Rats Experimentally Infected with Trypanosoma brucei brucei اثر تجویز خوراکی دوزهای درجه بندی شده دانه اوله فرای مورنیگا بر روی پاتولوزی بیضه ها ذخایر گنادی و خارج گنادی اسپرم رت های نزاد ویستارآلوده شده به تریپانوزوما بروسه ای بروسه ای 369 377 82897 10.22059/ijvm.2021.318812.1005160 EN Dauda Iliyasu Department of Theriogenology, Faculty of Veterinary Medicine, University of Maiduguri. Nigeria. Mohammed Abdullahi Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Maiduguri. Nigeria Adama Musa Abdullahi Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri. Nigeria Fatima Lawan Adamu Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri. Nigeria. Journal Article 2021 05 23 BACKGROUND: Moringa oleifera seeds are known for their high protein and vitamin content. Antioxidants are abundant in these seeds. Aqueous extraction was done. After that, an acute toxicity test was performed. OBJECTIVES: The purpose of the study was to see how a graded dose of M. oleifera aqueous seed extracts altered testicular pathology, gonadal and extragonadal sperm reserves in Wistar rats infected with Trypanosoma brucei brucei. METHODS: The rats were A, B, C, D, and E were randomly assigned to five groups with group E serving as the control group. The rats in Groups A to D were inoculated intra-peritoneal with 1× 106 virulent T. brucei brucei, and they were held for one week to demonstrate clinical signs before starting the extract therapy. Every day at 10:00 a.m., the rats were given treatment for five weeks with (75, 100, 125, and 150) mg/kg of M. oleifera aqueous seed extract for groups A, B, C, and D respectively. While the control group E received 0.5 mL/kg of water. For hema-tological indices, blood samples were collected every Monday between 10:00 and 11:00 a.m. All of the rats were humanely sacrificed at the end of the six-week experiment, and their gonadal and extragonadal sperm stores were collected, tested, and processed for histopathology. RESULTS: After treatment, the rats' gonadal and extragonadal sperm reserves (groups A to E) showed a substantial increase (213±1.1a; 221±2.1; 250±0.0c; 259±2.6d; 295±2.6e) × 106 and (115±1.1; 160±2.1; 153±0.0; 167±2.6; 120±0.6) × 106 respectively, compared to control group at P < 0.05 level. Sperm concentration of the right epididy-mis (60.0±1.1a; 90.2±2.1b; 96.5±0.0c; 98.7±2.6d; 69.4±0.6e) × 106 were significantly higher compare to the left epididymis (55.0±1.1; 69.8±2.1; 56.5±0.0; 68.3±2.6; 50.6±0.6) × 106. The PCV (%) and WBC (103/μL) levels in groups A, B, and C were significantly greater following infection than that in group E. Infection with T. brucei at weeks 2 and 3 shows poor semen characteristics, thereafter the semen quality has improved. CONCLUSIONS: Moringa oleifera aqueous seeds extract has drastically abridged the impact of trypanosomosis and enhanced the semen quality of the experimental rats.

https://ijvm.ut.ac.ir/article_82897_8fb12c56e0fa016c738e5763faa7237b.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 09 23 Drug Resistance Pattern of Pseudomonas aeruginosa Isolates Carrying MexAB-OprM Efflux Pump’s Associated Genes in Companion Birds with Respiratory Infection الگوی مقاومت داروئی جدایه های سودوموناس اروژینوزا (Pseudomonas aeruginosa) حامل ژن های مرتبط با پمپ برون ریز MexAB-OprM بدست آمده از پرندگان زینتی درگیر عفونت تنفسی 378 386 82769 10.22059/ijvm.2020.295678.1005051 EN Niloofar Meamar Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Jamshid Razmyar Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Seyed Mostafa Peighambari Department of Avian Diseases Faculty of Veterinary Medicine University of Tehran, Tehran, Iran Azam Yazdani Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2021 03 01 BACKGROUND: Pseudomonas aeruginosa is considered one of the most common bacterial pathogens causing nosocomial infections in human cases. However, the pathogenesis of this bacterium in companion birds is poorly understood. OBJECTIVES: The aim of the present study was to isolate P. aeruginosa from pet birds with respiratory illness manifestations referred to the clinic of the University of Tehran. Moreover, the antimicrobial susceptibility of the recovered P. aeruginosa isolates carrying MexAB-OprM efflux pump was evaluated. METHODS: Selective media and biochemical tests were used to isolate and identify P. aeruginosa isolates from 126 companion birds. The species-specific polymerase chain reaction (PCR) based on the 16S rRNA gene was applied to confirm P. aeruginosa. In addition, the sensitivity of isolates to 20 antimicrobial agents was assessed by an antimicrobial susceptibility test. Multiplex PCR was used to detect genes associated with MexAB-OprM efflux pump by specific primers in recovered P. aeruginosa isolates. RESULTS: All seven isolates identified as P. aeruginosa in culture by biochemical tests were confirmed utilizing species-specific PCR. The results of the antimicrobial susceptibility test demonstrated multidrug resistance (MDR) among the isolates with the highest resistance to neomycin, kanamycin, rifampicin, and vancomycin (100% of iso-lates) followed by colistin (57% of isolates). The mexA and oprM genes were detected in all isolates by multiplex PCR, while the mexB gene was not amplified in any of the seven isolates. CONCLUSIONS: We found P. aeruginosa isolates in sick birds and observed MDR in these isolates. Therefore, companion birds could be considered a potential public health concern, especially for owners and veterinary staff.

https://ijvm.ut.ac.ir/article_82769_cf2e31e949e87d9582c865ed09a12540.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Efficacy of Rev-1 Vaccine Against Brucella melitensis Infection in Dog مطالعه تجربی در رابطه با ایمنی زایی واکسن Rev-1 علیه عفونت بروسلا ملی‌تنسیس در سگ 387 394 82774 10.22059/ijvm.2021.309899.1005126 EN Hosein Esmaeili Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Ameneh Sadat Mahdavi Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Mona Hamedi Graduated for the Degree of Doctorate of Veterinary Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2021 04 06 BACKGROUND: Canine brucellosis may occur due to Brucella spp. other than Brucella canis. Brucella bacterium is transferred between dogs, ruminants, and humans. Therefore, there is a need for vaccinating the hosts of Brucella, especially dogs. OBJECTIVES: The present study evaluates the efficacy of Rev.1 against B. melitensis in experimentally infected dogs. METHODS: Twelve Brucella-negative dogs were divided into two groups of test and control. The animals in the experimental group were vaccinated with Rev.1. After vaccination, sera of the dogs were tested by the standard tube agglutination test (STAT) and Rose Bengal test (RBT). Five months following vaccination, dogs in both groups were inoculated with 3×109 CFU of B. melitensis biotype 1. Serum samples were taken after inoculating the bacteria and were examined using the STAT and RBT. The specimens of lymph nodes and reticuloendothelial organs were collected for bacteriological culture. RESULTS: After the inoculation of Brucella, the antibody titer was significantly higher in the control dogs than in the experimental group. B. melitensis biotype 1 was isolated from all the control dogs, but it was isolated from three dogs in the test group. CONCLUSIONS: According to the findings of the current study, we recommend further studies on the immuniza-tion of dogs with the Rev.1 vaccine along with vaccinating small ruminants.

https://ijvm.ut.ac.ir/article_82774_69a181f7f543ad69ce446ce438399f82.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Correlation Between the Transcriptional Level of iss and bor Virulence Genes of Avian Pathogenic Escherichia Coli O78 Inoculated to Chicken Serum همبستگی بین مقادیر رونوشت برداری ژن های حدت افزایش دهنده بقای سرمی و خوانش باز آبی اشریشیاکلی بیماریزای طیور O78 تلقیح شده در سرم جوجه گوشتی 395 403 82776 10.22059/ijvm.2021.310042.1005129 EN Somaye Abbasi Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Saeed Salari Department of Pathology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Ahmad Rashki Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Mohsen Najimi Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Journal Article 2021 04 16 BACKGROUND: Blue Open Reading (bor) and increased serum survival (iss) genes, with intense structural and possible functional similarities, engage in the serum resistance of Avian Pathogenic Escherichia coli O78 (APEC-O78). OBJECTIVES: This research aimed to determine whether there is a correlation between the transcriptional level of bor and iss of APEC-O78 inoculated in serum. METHODS: Total RNA was extracted 24, 28, and 32 hrs after the inoculation of an APEC-O78 isolate, χ1378, to chicken serum. The fluorescence intensities related to the bands of gel electrophoresis of bor and iss were computed after cDNA synthesis and reverse transcription -PCR reaction assay. Using Pearson’s partial correlation tests, the relationship between the transcriptional levels of bor and iss, and the influence of the selected time points on the possible relationship were respectively measured at P<0.05 for statistical significance. RESULTS: A correlation was observed between the transcription levels of bor and iss (P=.012), which was not influenced by the selected time points (P=.001). CONCLUSIONS: The bor must be carefully weighed in the transcriptional analysis including iss and vice versa. The number of transcripts/alleles, upstream sequence, regulation process at the pre-transcriptional phase, and the location of bor and iss in χ1378, and taking samples from different time intervals may explain our result. Our find-ings emphasizes the multifactorial and complex mechanism of APEC-O78 serum resistance, and can lay the foundation for further studies on the transcription of bor and iss, particularly in the specific strain and the three time points.

https://ijvm.ut.ac.ir/article_82776_7e32a913d51d157591a5e7b7b0c0346e.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Purinergic Regulation of Food and Fat Intakes in Broiler’s Central Nervous System تنظیم پورینرژیک اخذ غذا و چربی در سیستم عصبی مرکزی جوجه های گوشتی نوزاد 404 410 82775 10.22059/ijvm.2021.309901.1005127 EN Sahel Motaghi Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman Hossein Jonaidi Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman Alireza Bashiri Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran Samaneh Nouri Gooshki Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman Journal Article 2021 02 08 BACKGROUND: Adenosine has many physiological roles in the brain, and in rodents, it changes food intake when applied centrally. OBJECTIVES: We investigated the effect of central injection of the purine molecule adenosine on both food and fat intakes in neonatal chicks. METHODS: In the first trial, various doses of adenosine (an endogenous P1 receptor agonist), and its synthetic antagonist CGS-15943, were injected intracerebroventricularly (ICV) to the chicks and the cumulative food intake was measured at definite time intervals. The second trial was similar to the first one, only the chicks were fed with a high-fat diet. RESULTS: Adenosine did not affect food or fat intake. Food consumption was increased 30 min after injection of CGS-15943. CGS-15943 also increased fat intake in chicks fed a high-fat diet. CONCLUSIONS: The present study suggests that in the avian central nervous system, P1 receptors are entailed in the regulation of food and fat intake in an antagonistic manner.

https://ijvm.ut.ac.ir/article_82775_07fa749558f3f213e60ef3f94676136d.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Effects of Parsley (Petroselinum Crispum) Hydroalcoholic Extract on Spermatogenesis and Pituitary- Gonadal Axis in Streptozotocin-Induced Diabetic Male Rat بررسی اثر عصاره هدروالکلی گیاه جعفری بر اسپرماتوژنز و محور هیپوفیز- گناد موشهای نر دیابتی شده با استرپتوزوتوسین جواد چراغی ، پریسا کریشچی خیابانی، سیما نصری، مریم زرگوشی 411 422 82778 10.22059/ijvm.2021.312948.1005139 EN Javad Cheraghi Department of Veterinary Laboratory Sciences, Faculty of Para-Veterinary Medicine, Ilam University, Ilam, Iran Parisa Kridhchi MSc of Biology, Payam-e Noor University, Tehran, Iran Sima Nasri Department of Biology, Faculty of Basic Sciences, Shahr-e Qods Branch, Islamic Azad University, Tehran, Iran Maryam Zargooshi Department of Biology, Faculty of Sciences, Payam-e Noor University, Tehran, Iran Journal Article 2021 01 31 BACKGROUND: Diabetes mellitus (DM), as a metabolic disease, has a high rate of mortality all over the world. In recent years, there is accumulating evidence that show this complex multifactorial disease has various effects on reproductive system. OBJECTIVES: This study aimed to investigate the effect of hydroalcoholic extract of parsley (Petroselinum cris-pum) leaves on spermatogenesis and pituitary-gonadal axis in male streptozotocin (STZ)-induced diabetic rats. METHODS: In the present study, 60 male rats were divided into 5 groups of 12 animals in each: control, DM control and 3 experimental groups (treated with doses 1, 2, and 4 gr/kg of hydroalcoholic extract of parsley leaves gavage for 28 days). DM was induced 48 h after intraperitoneal injection of a single dose of STZ (65 mg/kg). At the end of the treatment, rats were bled from the heart and follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels were measured. The testes of rats were isolated and weighed. Assessment of the sperm motility and evaluation of the seminiferous cells were performed using light microscopy. RESULTS: Comparison of LH, FSH and testosterone in rats treated with doses 1 mg/kg of hydroalcoholic extract of the plant revealed a significant increase as compared to the diabetic control group (P≤0.05). In addition, the results of histopathological evaluation elucidated a significant increase in the sperm cells number, and motility, testis weight, and total sperm count. It also improved condition of the seminiferous cells in the experimental group (1 mg/kg) compared to the diabetic control group (P≤0.001). CONCLUSIONS: It seems that parsley (P. crispum) hydroalcoholic extract can be effective in decreasing repro-ductive disorders in DM patients.

https://ijvm.ut.ac.ir/article_82778_1f84f5d991424bfccef88e367ef04011.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Conception Rate of Pre-synchronization and Two Short Term Heat-synch Programs using Two Doses of PGF2α in Lactating Holstein Dairy Cows میزان آبستنی متعاقب برنامه پیش همزمانی و دو برنامه هیتسینک کوتاه مدت با استفاده از ۲ تزریق PGF2α در گاوهای شیری 423 431 82785 10.22059/ijvm.2021.319206.1005162 EN Hamid Ghasemzadeh-nava Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran Mirmoein Bahrami Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran Vahid Akbarinejad Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran Seyed Alireza Alavi Tabatabaee Theriogenologist and Private Practitioner, Tehran-Iran Journal Article 2021 03 08 BACKGROUND: Improving the reproductive performance of dairy cows requires estrus manipulation using estrus synchronization protocols. OBJECTIVES: The current study aimed to assess the effect of reducing the length of estrus synchronization pro-tocol by two doses of prostaglandin injection on the reproductive performance of Holstein dairy cows. METHODS: At first, all cows received GnRH (day 0) followed by PGF2α on day 7 and GnRH on day 17. Next, cows were assigned randomly to one of the three groups: Group 1) PGF2α injection on days 22 and 23, estradiol benzoate injection on day 24, Group 2) PGF2α injection on days 23 and 24, estradiol benzoate injection on day 25, and Group 3) Control group; injection of PGF2α on day 24, injection of estradiol benzoate on day 25. Estrus detec-tion was performed twice a day up to 48 h (AM/PM) based on the signs of estrus, and fixed time AI protocol was used after 48 h if not previously inseminated. RESULTS: The conception rate in group 2 was significantly higher than the control group (P≤0.05) and tended to be higher than group 1 (P=0.079). CONCLUSIONS: Our results confirmed the hypothesis that a decline in the period of follicle dominance by re-ducing the interval between GnRH and PGF2α through two PGF2α injections improved the fertility of lactating dairy cows.

https://ijvm.ut.ac.ir/article_82785_90de7c86a86f37f87cb510ce204a51a9.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Threshold Time to Onset Serum Biochemical Changes of Turkoman Racehorses at Different Serum-Clot Contact Times and Temperatures زمان آستانه شروع تغییرات بیوشیمیایی اسب های مسابقه ای ترکمن در زمان ها و دما های مختلف تماس سرم - لخته 432 439 82772 10.22059/ijvm.2021.308928.1005124 EN Samareh Kamali Sadeghian Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran. Mahmood Ahmadi-hamedani Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Mohammad Hassan Yousefi Department of Basic Sciences, Faculty of Veterinary Science, Semnan University, Semnan Reza Narenji Sani Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Journal Article 2021 04 14 BACKGROUND: It is essential to minimize the effect of time and temperature on the serum biochemical param-eters and determine the stability limits of each analyte in pre-centrifuged blood samples. OBJECTIVES: This study aimed to investigate the stability limits of 10 analytes in Turkoman racehorses blood samples stored in different temperatures and time points. METHODS: The whole blood samples from healthy horses (n=10) were stored for 2 h (baseline), 6, 12, 24, and 48 h at 25ºC or 4ºC. The commercial kits (Parsazmoon, Tehran, Iran) were used for the samples analysis. RESULTS: Albumin (ALB), total bilirubin (TB), and phosphorous (P) exhibited remarkable changes at 25ºC. The storage time for as long as 12 h at 25ºC had no significant effect on urea, total protein (TP), lactate dehydrogenase (LDH), creatinine, and alkaline phosphatase (ALP). The stability of alanine transaminase (ALT) in serum samples stored at 25ºC was for 24 h and for LDH was for 48 h at 4ºC. Aspartate transaminase (AST) was the most unstable analyte at different storage times at both temperatures. Urea, TP, ALB, TB, and P were stable at 4ºC for as long as 6 h. Creatinine and ALP were affected by 24 and 48 h storage times at both temperatures. There was a significant difference (P<0.05) in AST and ALT activities between two temperatures. No significant difference was observed in creatinine, urea, and TB concentrations between two storage temperatures at any of the storage times. CONCLUSIONS: This study showed that some analytes have acceptable stability in the clotted blood samples stored at 4°C for 6 h.

https://ijvm.ut.ac.ir/article_82772_ef714399e52505eb1dff92f265e336bf.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Investigating the Effect of Hydroalcoholic Extract of Eryngos on Plasma Concentration of Blood Glucose, Blood Cells and Pancreatic Tissue in Diabetic Rats بررسی اثر عصاره هیدروالکلی بوقناق برغلظت پلاسمایی قند خون ، سلول‌های خونی وبافت پانکراس دررت های دیابتی 440 451 82777 10.22059/ijvm.2021.311523.1005134 EN Elham Moghtadaei Khorasgani بخش پاتوبیولوژی واحد شهرکرد Abbas Khani ... Journal Article 2021 03 10 BACKGROUND: Diabetes mellitus is one of the most common metabolic disorders with a severe impact on the quality of life. OBJECTIVES: The objective of the present study was to investigate the impact of the hydroalcoholic extract of Eryngos on blood glucose, blood cells, and pancreatic tissue. METHODS: Thirty-five male rats were prepared. After diabetes induction by streptozotocin, they were randomly divided into 5 groups, including; non-diabetic control, diabetic control, diabetic treated with hydroalcoholic extract of Eryngos at doses of 300 and 500 mg/kg administered intraperitoneally, and metformin at a dose of 500 mg/kg. At the end of the study, glucose level was measured, and blood cells and pancreatic tissue were examined. RESULTS: Hydroalcoholic extract of Eryngos caused a significant reduction in blood glucose. Given the adverse effects of diabetes on the number of WBC, Eryngos extract at a dose of 300 mg/kg had a protective effect on the number of WBC. It decreased their number significantly compared with the diabetic control group. This effect was the same for both diabetic and non-diabetic groups. Histopathological results also indicated that Eryngos extract significantly increased the number of islets of Langerhans and beta cells. CONCLUSIONS: The results suggest that the hydroalcoholic extract of Eryngos may be effective in the treatment of diabetes. Moreover, based on the biochemical and histological results, it can be concluded that the hypoglycemic effect of the extract is probably due to the restoration and repair of the islets of Langerhans

https://ijvm.ut.ac.ir/article_82777_2a3fdf2b998bc9a7fb320f892a7823d4.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 15 4 2021 10 01 Clinical, Radiological and Microscopical Findings of Chondrosarcoma in a Cat یافته های بالینی، رادیولوژی و میکروسکوپی کندروسارکوم در یک گربه 452 456 82784 10.22059/ijvm.2021.318789.1005158 EN Sara Shokrpoor Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Behzad Pourreza Department of Radiology and Surgery, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Mina Khodadadi Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Masoumeh Moradi-Ozarlou Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Reza Khoshvaghti Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Bita Fazel Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2021 02 13 Chondrosarcoma is an uncommon malignant neoplasm in which the neoplastic cells produce chondroid and varying amounts of matrix. This tumor is the second most common primary skeletal tumor in animals. In our case, radio-logical and histopathological findings supported the final diagnosis of chondrosarcoma in a six-year-old male cat weighing 6.3 kg with a large and solitary mass in the right humerus. The shoulder joint had a limited range of motion. Radiographs were obtained from the raised growth. The mass was surgically excised for histopathological evaluation. Microscopically, the mass was composed of bundles and nests of neoplastic mesenchymal cells. Histo-pathologically, multiple-sized lacunae within a homogenous cartilage matrix were observed. There was marked anisocytosis and anisokaryosis. Neoplastic chondrocytes and oval cells with 1 to 3 nucleoli were seen. Based on the macroscopic, radiological, and cartilaginous differentiation of tumor cells and matrix, the mass was diagnosed as well-differentiated chondrosarcoma

https://ijvm.ut.ac.ir/article_82784_3f3a7e436e97cd30c36bc62f9535bc93.pdf