University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Trichinellosis Prevalence and Distribution Flow of Wild Boar Meat in West Pasaman Regency, West Sumatera Province, Indonesia 169 178 101508 10.32598/ijvm.19.2.1005677 EN Yasir Hamdani Dalimunthe Animal Biomedicine Study Program, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia. Fadjar Satrija Animal Biomedicine Study Program, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia. Yusuf Ridwan Animal Biomedicine Study Program, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia. Sri Murtini Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia. Etih Sudarnika Division of Veterinary Public Health and Epidemiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia. Journal Article 2024 11 09 Background: Trichinellosis is a zoonotic disease transmitted by the consumption of undercooked meat infected with Trichinella larvae. Wild boars are significant reservoirs of this pathogen, posing health risks to humans, particularly in regions where hunting is prevalent. Wild boar meat infected with larvae Trichinella spp. has an increased risk of human infection with trichinellosis. Objectives: This study aimed to measure the prevalence of trichinellosis in wild boars and analyze the wild boar meat distribution flow in West Pasaman Regency, West Sumatera Province, Indonesia. Methods: Muscle samples were collected from 106 wild boars that were captured during a traditional hunting event. The samples were obtained from four anatomical sites (masseter, forelimb, diaphragm, and intercostal muscles), pooled, and tested for antibodies against Trichinella excretory/secretory antigens using indirect enzyme-linked immunosorbent assay (ELISA). Data on meat distribution were gathered through interviews with hunters, collectors, traders, and authorized officers, using a structured questionnaire. Results: ELISA results showed that 48(45.28%) of the 106 wild boar samples tested positive for Trichinella spp. There was no significant correlation between seropositivity against sex (P=0.503) and body weight (P=0.485) of wild boars. The interview results showed that the captured wild boars were collected by local small-scale collectors and sold the meat either to local consumers or large-scale collectors. Local large-scale collectors sold meat both inside and outside the West Pasaman Regency. Large-scale collectors outside West Pasaman Regency sold meat to traders or directly to consumers. The Plantation and Livestock Services of West Pasaman Regency did not know about the distribution of the resulting wild boar meat or inspect this product. Distribution flow analysis showed that wild boar meat is consumed locally and sold outside the region and province. Conclusion: This study revealed a relatively high prevalence of trichinellosis in wild boars in the West Pasaman Regency, West Sumatra Province, Indonesia. Hunted wild boars were distributed to consumers through various actors in the West Pasaman Regency and beyond. Such conditions raise concerns about the health implications for consumers. Therefore, it is important to increase public awareness regarding safe food practices and conduct surveillance of wildlife and livestock health in the region.

https://ijvm.ut.ac.ir/article_101508_ffa21c6096df7128f187e89fb8fd49e4.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Molecular and Clinical Study of Feline Infectious Peritonitis Virus in Iran Showing a Paraphyletic Tree: Emphasizing the “Internal Mutation” Hypothesis 179 190 97622 10.32598/ijvm.19.2.1005247 EN Shahram Jamshidi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Farnoosh Momeni Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Iradj Ashrafi Tamai Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Omid Madadgar Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Journal Article 2023 07 08 Background: Feline infectious peritonitis (FIP) is a severe and often fatal disease affecting feline species. Despite the high prevalence of feline coronavirus (FCoV) infections, the manifestation of FIP occurs in only a small percentage (1%-5%) of cases. The intricate aspects of FIP differential diagnosis persist, and a comprehensive understanding of the molecular mechanisms driving FIP pathogenesis remains elusive. Objectives: This study aims to thoroughly investigate the characteristics of Iranian feline infectious peritonitis viruses (FIPV), encompassing sequence analysis and detailed examination of laboratory and clinical findings. The primary objective is to unravel the hypothesized genesis of the FIP virus, with a specific focus on the membrane (M) gene level. Methods: Our methodology involved examining abdominal or thoracic fluids from 17 cats suspected of having FIP, utilizing biochemical tests, such as total serum protein, albumin to globulin (A/G) ratio, and the Rivalta test. A molecular approach utilizing reverse transcription-polymerase chain reaction (RT-PCR) based on the M gene was employed. Sequence analysis of five crucial residues in the M genes and subsequent construction of a phylogenetic tree using the five sequenced viruses further enriched our investigation. Results: The study confirmed FIP in 6 of 17 cats through the Rivalta test, guiding subsequent evaluations. Significant gender disparities in FIP occurrence were observed among young cats (9-30 months old), with males exhibiting a two-fold higher incidence than females. Affected cats within the 9-30 months age range consistently exhibited an albumin to globulin (A/G) ratio below 0.66 and total serum protein exceeding 0.43 g/dL. Cavity fluid cytology indicated non-degenerated macrophages and neutrophils against a basophilic background due to a high protein percentage, confirming FIP diagnosis. Importantly, sequence analysis of five M protein amino acid hotspots revealed negligible differences in nucleotide sequences between feline enteric coronavirus (FECoV) and FIPV, aligning with their biotypic patterns. Conclusion: The phylogenetic tree generated in this study displayed a paraphilic pattern, emphasizing the “internal mutation” hypothesis, suggesting that viral mutations occur within the cat’s body and no significant differences are observed in FECoV and FIPV-generating viruses. These results provide valuable insights into the discourse surrounding FIP pathogenesis, potentially guiding future diagnostic and therapeutic approaches.

https://ijvm.ut.ac.ir/article_97622_bc05ac17a999972ca8cd4d4c75692546.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Effect of Coumestrol Supplementation on Ovine Semen Cryopreservation 191 202 97606 10.32598/ijvm.19.2.1005551 EN Doaa Hosny Elsayed Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. Laila Esmael Kortam Immunity Unit, Agriculture Research Center (ARC), Animal Reproduction Research Institute, Giza, Egypt. Heba Mohamed Ahmed Abdelrazek Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. Ahmed Monir Department of Artificial Insemination and Embryo Transfer, Agriculture Research Center (ARC), Animal Reproduction Research Institute, Giza, Egypt. Journal Article 2024 04 27 Background: Antioxidant supplementation promotes the fertilizing capacity of post-thawed ram spermatozoa. Objectives: This study was designed to validate the effect of different levels of coumestrol as an antioxidant on post-thawing parameters of cryopreserved spermatozoa in rams. Methods: A total of 60 ejaculates were collected from six sexually mature Barki rams. The accepted semen samples were extended, pooled, cooled, and then divided into five aliquots: A control group (tris-based egg yolk extender) without coumestrol addition, and 4 aliquots with concentrations of 0.1, 1, 10, and 100 µM of coumestrol. These aliquots were then subjected to the cryopreservation process. The control and treated frozen semen were thawed and assessed for motility using computer-assisted sperm analysis (CASA), total antioxidant capacity (TAC), malondialdehyde (MDA) levels, mitochondrial activity, comet assay for DNA integrity, acrosome integrity, and casein kinase 2 alpha 2 (CK2α2) gene expression. Results: The addition of 1 µM of coumestrol significantly improved progressive motility. Metabolic activity was significantly higher in semen treated with 1, 10, and 100 µM coumestrol compared to control and 0.1 µM coumestrol groups. Furthermore, acrosome integrity was significantly higher in the 0.1 and 1 µM of coumestrol groups. In the 1, 10, and 100 µM coumestrol groups, TAC was significantly higher than in controls. Furthermore, MDA levels were significantly lower in all coumestrol groups compared to the controls. The comet assay exhibited a significant reduction in fragmented DNA in semen treated with coumestrol, especially with the addition of 1 µM coumestrol. The expression of CK2α2 showed a significant fold decline in semen supplemented with 10 and 100 µM coumestrol compared to the control group. Conclusion: The addition of 1 µM of coumestrol could ameliorate the deleterious impacts of cryo-damage by improving the sperm antioxidant capacity, mitochondrial activity, and both acrosome and DNA integrity.

https://ijvm.ut.ac.ir/article_97606_07f1925accb356e6fa9398b5ca8416a7.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Investigation of the Vaginal and Clitoral Fungi in One-Humped Camels (Camelus Dromedarius) 203 210 94363 10.32598/ijvm.19.2.1005423 EN Hosein Kochakzadeh Omran Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran. Tehran, Iran. Massoud Talebkhan Garoussi Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran. Tehran, Iran. Ali Reza Khosravi Mycology Research Centre, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Jorgen Steen Agerholm Department of Veterinary Clinical Sciences, University of Copenhagen, Taastrup, Denmark. Mehran Dabiri Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran. Tehran, Iran. Journal Article 2023 08 05 Background: Bacteria and fungi constitute crucial parts of the genital tract microenvironment and participate in mucosal homeostasis. While the genital tract microflora has been studied for several animal species, this is not true for the one-humped camel (Camelus dromedarius). Objectives: This survey aims to investigate the prevalence of vaginal and clitoral fungi in one-humped camels (C. dromedarius ) from different regions of Iran. Methods: Dairy camel farms were sampled in Khorasan Razavi, Semnan, Tehran, Qom, and Isfahan Provinces in central and northeast Iran. Two hundred and twenty paired samples were taken from the vaginal and clitoral mucosa of 110 camels and cultured on Sabouraud dextrose agar and CHROMagarTM Candida, followed by identification of cultured fungi. Data were analyzed using the Genmod procedure. Results: Six isolates were identified. Fungi were cultured and isolated from 31% of the camels. Cladosporium spp. and Aspergillus flavus were the most isolated prevalent. Aspergillus was the most frequently recovered species. The presence of fungi was evaluated in camels of different ages, but no correlation was observed (P>0.05). Conclusion: Fungal contamination of the camel's external organs of the reproductive system is very low compared to other domestic animals. It is likely due to the intrinsic immunity of one-humped camel (C. dromedarius).

https://ijvm.ut.ac.ir/article_94363_56a43c223f4feff18f7fa6ff7dec6bde.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Effect of Rhazya stricta-synthesized Copper Nanoparticles on Staphylococcus aureus-infected Wounds in Rabbit 211 226 100095 10.32598/ijvm.19.2.1005593 EN Ali Hussein Aldujaily Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Kufa, Kufa, Iraq. Kifah Fadhil Hassoon Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Kufa, Kufa, Iraq. Douaa Barzan Salman Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Kufa, Kufa, Iraq. Ghadeer Sabah Bustani Department of Anesthesia, Faculty of Medical Technologies, The Islamic University, Najaf, Iraq. Journal Article 2024 06 07 Background: Nanoparticles (NPs) are utilized in various technological fields, including medicine, due to their inherent antibacterial properties. Recent research has focused on the biosynthesis of copper NPs (CuNPs) and their potential medical applications. Objectives: This study aimed to use Rhazya stricta for the green synthesis of CuNPs and assess their effectiveness in eradicating bacterial pathogens, particularly Staphylococcus aureus, and promoting wound healing in rabbits. Methods: The synthesized NPs were characterized using UV-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), x-ray diffraction (XRD), atomic force microscopy (AFM), and zeta potential analysis. Fifteen rabbits were divided into three groups of five. Full-thickness costo-abdominal skin wounds were created on the right side of each rabbit. The first group served as the untreated control, the second group was treated with CuNPs, and the third group received fusidic acid treatment. Results: R. stricta extract successfully synthesized CuNPs. The application of CuNPs on S. aureus-contaminated wounds showed faster healing than fusidic acid treatment. The CuNPs group healed in 16 days, while the fusidic acid group healed in 22 days. CuNPs-treated wounds had significantly reduced wound area, total cell count, neutrophil count, macrophage count, and lymphocyte count (P<0.05), along with increased wound contracture (P<0.05). Bacterial counts indicated that CuNPs eradicated S. aureus infections in seven days, compared to 12 days for fusidic acid. CuNPs reduced inflammation and promoted collagen fiber deposition, leading to better healing of S. aureus-infected wounds by decreasing hemorrhagic regions and inflammatory cells. Conclusion: CuNPs synthesized using R. stricta show promising potential as a safe and effective treatment for infected wounds. They effectively eradicate infections and promote efficient wound healing, making them a viable therapeutic option for managing infected wounds.

https://ijvm.ut.ac.ir/article_100095_a6151b241d9e0451ed63303a25f674c5.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Clinical Presentation and Diagnosis of Anthrax in Iranian Goats 227 236 96954 10.32598/ijvm.19.2.1005510 EN Hossein Esmaeili Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Seyed Mehdi Joghataei Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Fatemeh Haji Agha Khiyabani Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Journal Article 2024 01 28 Background: Anthrax is considered one of the most important zoonotic diseases due to its widespread distribution, significant economic losses, and potential risks to public health. The manifestation of anthrax in goats is comparable to its presentation in other small ruminants. Objectives: To enhance understanding of the clinical signs and post-mortem findings of anthrax and improve its diagnosis in the goat population of Iran, this study examined the occurrence of anthrax in goats in some provinces of Iran between 2016 and 2021. Methods: Among a population of 3,465 goats, 50 cases of sudden death were documented. Samples were collected from recently deceased animals, including bone marrow and blood from ear veins. These samples were sent to the laboratory for direct examination, culture, and molecular assay (polymerase chain reaction [PCR]). Results: Out of the collected samples, 36 cases were attributed to anthrax. Among these deaths, 27 animals were found dead, while nine goats were slaughtered before succumbing. Bacillus anthracis was confirmed in all 36 suspicious samples using direct examination and PCR. Additionally, 11 samples (30%) were found to be culture-positive for anthrax. It is worth noting that classic signs of anthrax, such as unclotted blood oozing from natural orifices and the absence of rigor mortis, were not observed in 19 dead goats. Conclusion: This study reveals that there are instances of anthrax in goats where the typical clinical signs, such as blood oozing from natural body orifices, are absent. This finding should be considered during the differential diagnosis, especially when the classic signs are used as criteria.

https://ijvm.ut.ac.ir/article_96954_2be798dbb2706f8a421d3010b1db7798.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 The Inhibitory Effect of Camel Lactoferrin-chimera, a Recombinant Antimicrobial Peptide, on Avian Influenza Virus Subtype H9N2 237 254 95719 10.32598/ijvm.19.2.1005499 EN Moein Khodayari Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Mohammad Hadi Sekhavati Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Seyed Mostafa Peighambari Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Abbas Barin Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Omid Dezfoulian Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran. Jamshid Razmyar Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Journal Article 2023 12 27 Background: Avian influenza subtype H9N2 is poultry’s most prevalent influenza virus worldwide. It imposes economic losses to the poultry industry and has zoonotic potential. Currently, the two main groups of anti-influenza drugs are adamantanes and neuraminidase inhibitors. In recent years, there has been an increase in the resistance to existing anti-influenza drugs. Antimicrobial peptides are a group of potential drug candidates with broad-spectrum activity. Camel lactoferrin (cLF)-chimera is an antimicrobial peptide synthesized from camel milk lactoferrin. Objectives: This study aims to evaluate the inhibitory effects of cLF-chimera on avian influenza, subtype H9N2. Methods: One hundred and seventy 11-day-old specific pathogen-free (SPF) embryonated eggs were randomly distributed into 17 groups. Different virus and peptide concentrations were injected into the eggs. The eggs were incubated for four days, and daily candling was done for viability assessment. On the 4th day of incubation, each group’s live or dead embryos were sorted and evaluated for gross anomalies. Next, for histopathological analysis, chick embryos were fixed with 10% neutral buffered formalin for one week. The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay was performed to determine peptide and virus concentrations. Results: Embryo viability results and macroscopic and histopathologic findings showed that the peptide had inhibitory effects against the virus. These results are consistent with the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (MTT) assay. Moreover, the peptide has proven effects against pathogenic bacteria, which can be advantageous compared to common anti-influenza medications. Conclusion: According to the results, cLF-chimera has an inhibitory effect on the H9N2 influenza virus.

https://ijvm.ut.ac.ir/article_95719_9f56758f82009000e025e01933cba49c.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Nutrient Digestibility Coefficients and Carcass Evaluation of Japanese Quails to Aqueous Administration of Egg Lime Molasses Mixture 255 264 98572 10.32598/ijvm.19.2.1005477 EN Adeyinka Oye Akintunde Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo, Nigeria. Lois Chidinma Ndubuisi-Ogbonna Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo, Nigeria. Olayinka Abosede Ojo Department of Animal Production Fisheries and Aquaculture, Kwara State University, Malete, Nigeria. Bolatito Adenike Shobo Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo, Nigeria. Olufunso Emmanuel Akinboye Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo, Nigeria. Osagie John Afodu Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo, Nigeria. Oluwafunmike Omowunmi Oyekale Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo, Nigeria. Journal Article 2023 11 26 Background: Japanese quails are cheap and easy to maintain, and they have the potential to bridge the gap in animal protein consumption shortage; however, their performance is highly dependent on the efficiency of nutrient utilization. Objectives: This study aimed to determine the effect of an egg-lime-molasses mixture (ELM) administration on Japanese quails’ nutrient utilization and carcass parameters. Methods: The study was conducted at the poultry unit of the Babcock University farmhouse, Ilishan-Remo, Ogun State, Nigeria, from January to March 2022. ELM was prepared by placing fresh chicken eggs in a bowl, after which one liter of lime juice and 500 g of molasses were added and covered tightly for 10 days at 27 °C with a relative humidity of 61%. The solution was then blended. Two-hundred-day-old Japanese quails were assigned to one of five treatments in a completely randomized design. The birds were subdivided into four replicates of 10 birds each. The control (T1) had no administration of ELM; T2, T3, T4, and T5 had inclusion levels of 10, 20, 30, and 40 mL, respectively, all in 500 mL of water. Food and water were provided ad libitum. Data on performance characteristics, apparent digestibility coefficients, and carcass parameters were collected and analyzed using SPSS software, version 22 using descriptive statistics and analysis of variance. Results: The results revealed that ELM did not significantly affect performance characteristics and carcass parameters (P>0.05). The values for digestible crude protein were significantly higher (P<0.05) in the control (62.99%). However, the lowest values were observed among birds administered the lowest dosages (10 and 20 mL ELM with 56.36% and 54.38%, respectively). Birds administered the lowest dosages of ELM (10 mL) had significantly higher (P<0.05) digestible lipids (55.50%) and digestible ash (23.41%) values. The birds administered 20 mL ELM had significantly least (P<0.05) values for digestible lipids (25.50%) and digestible ash (2.58%), while significantly highest (P<0.05) values were observed for the same group of birds for digestible crude fiber (41.05%) and digestible carbohydrates (38.79%). Conclusion: The inclusion of ELM in the water of Japanese quails did not alter their growth performance or carcass parameters.

https://ijvm.ut.ac.ir/article_98572_325e190583a8599ee9051f35f9a332ae.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Investigation of Probiotic Attributes and Aromatic Components Produced by Lactic Acid Bacteria Isolated From Iranian Traditional Yogurts 265 278 98899 10.32598/ijvm.19.2.1005497 EN Nassim Azari Department of Food Science and Technology, Faculty of Agriculture Sciences, Takestan Branch, Islamic Azad University, Takestan, Iran. Marjan Nouri Department of Food Science and Technology, Agriculture and Basic Sciences, Roudehen Branch, Islamic Azad University, Roudehen, Iran. 0000-0001-7028-8474 Journal Article 2023 12 23 Background: Yogurt is consumed in different ways all over the world owing to its high nutritional value, making it important to identify distinct strains from local producers with specific characteristics. Objectives: The purpose of the present study was to investigate the presence of probiotic bacterial populations in traditional yogurt, as an Iranian dairy product, and their effect on the probiotic specifications of yogurt. Methods: Initially, the isolation of lactic acid bacteria (LAB) was done using the culture method and then, the isolates were identified by examining their biochemical characteristics and 16S rRNA gene sequences. Finally, the characteristics of sensitivity to acidic conditions, bile salts, antimicrobial functions, survival rate, sensory properties, and aroma production for the isolates were evaluated. Results: Twelve isolates were identified from Lactobacillus and Enterococcus families. In general, the Lactobacillus plantarum strain KLDS 1.0725 exhibited the maximum ability to survive under acidic conditions. The L. plantarum strain KLDS 1.0725 and Enterococcus faecium strain FS019 had the highest survival in 0.3 and 0.5 % bile salts, respectively. L. plantarum WCFS1 and E. faecium Aus0004 created the maximum and minimum inhibition halos against all pathogens, respectively. The L. plantarum strain KLDS 1.0725 indicated enhanced abilities to produce acetaldehyde (25.59 ppm), while L. delbrueckii subsp. lactis illustrated the highest diacetyl production (5.96 ppm). The highest acceptability score in the sensory assessment was obtained for the L. plantarum strain KLDS 1.0725 and the E. faecalis strain V583. Conclusion: The overall results demonstrated the ability of isolated strains from yogurt to be applied in the industry, exhibiting desirable technological features and suitable aroma.

https://ijvm.ut.ac.ir/article_98899_79cce507abf7842798cb6126916dce4d.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Improving the Antioxidant Status, Meat Quality, and Immune Response of Broilers by Fermented Feather Meal 279 298 96864 10.32598/ijvm.19.2.1005483 EN Hassan Safari Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran. Ardeshir Mohit Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran. Maziar Mohiti-Asli Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran. Journal Article 2023 12 04 Background: Feather biodegradation is an effective alternative to other processing methods. Objectives: This study aims to investigate the effects of hydrolyzed feather meal (HFM) on broilers’ antioxidant status, meat quality, and immune response. Methods: In October 2022, 480-day-old Ross 308 male broilers were used for 42 days in a completely randomized design with eight treatments and five replicates (12 chicks/replicate) in the research farm of Agriculture Faculty, Guilan University (Rasht City, Iran). The experimental diets were as follows: (1) control diet (without feather meal [FM]), (2), (3), (4), (5), (6), (7), and (8) all containing 4% raw FM (RFM), HFM by autoclave (Au-HFM), fermented FM (FFM) by Bacillus licheniformis (Bl-FFM), FFM by Bacillus subtilis (Bs-FFM), FFM by Aspergillus niger (An-FFM), FFM by B. licheniformis+B. subtilis+A. niger (Co-FFM), and HFM by an enzyme (En-HFM), respectively. Results: The results of the in vitro experiment showed that 2,2-diphenyl-1-picrylhydrazl (DPPH) and 2, 2ʹ-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging activities, as well as microorganism count of FFM, increased with increasing fermentation time (P≤0.05). Also, the pH of FFM significantly decreased by increasing the fermentation time (P≤0.05). The DPPH and ABTS radical-scavenging activities of FFM were higher than those of RFM, Au-HFM, and En-HFM (P≤0.05). The in vivo experiment results showed that the total antioxidant capacity (TAC) serum levels, glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) in the FFM were higher than those in the control and other treatments (P≤0.05). Compared to control and other treatments, broilers fed FFM had lower meat malondialdehyde (MDA) levels, higher meat pH, and higher water-holding capacity at 0, 7, and 14 d of the breast meat storage, as well as lower serum MDA levels (P≤0.05). Broilers fed FFM had higher antibody titers against Newcastle disease virus, lower heterophil count, and lower heterophil-to-lymphocyte ratio, as well as higher total anti-sheep red blood cell (anti-SRBC) titer, immunoglobulin G (IgG), and immunoglobulin M (IgM), compared to the control and other treatments (P≤0.05). Conclusion: Fermentation of FM increased its antioxidant properties, improved the body’s antioxidative status, and contributing to improved the meat quality and immune response of broilers.

https://ijvm.ut.ac.ir/article_96864_34b4afe21798dc71df9613e459f48b20.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Oxytetracycline Residues in Eggs From Commercial Poultry Farms in Ilorin City, Nigeria 299 306 98900 10.32598/ijvm.19.2.1005536 EN Ibraheem Ghali-Mohammed Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. Shukrah Omotayo Ghali Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. Ismail Ayoade Odetokun Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. Ismail Adewuyi Adeyemo Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. Isaac Olufemi Olatoye Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria. Journal Article 2024 03 16 Background: The risk of indiscriminate antibiotic use in Nigeria is high and has serious public health and food implications. The unrestricted usage of oxytetracycline can lead to the accumulation of antibiotic residues in animal products, such as eggs. Objectives: This study investigated oxytetracycline residues in eggs from 20 commercial poultry farms in Ilorin City, Nigeria. Methods: Samples were randomly collected from poultry farms in Ilorin City, Nigeria, and analyzed using high-performance liquid chromatography (HPLC). Results: Oytetracycline residues were detected in pooled egg samples from 15 farms (75% prevalence). The mean concentration of 398.30±186.73 µg/kg was obtained with six samples (30%) exceeding the recommended maximum residue limit (MRL) of 400 µg/kg. Standard curve analysis showed linearity (r2=0.98). Conclusion: This study revealed a high prevalence of oxytetracycline residues in eggs produced by commercial poultry farms in Ilorin City, Nigeria. It underscores the need for better regulation and oversight of antibiotic use in poultry farming to mitigate health risks associated with antibiotic residues and antimicrobial resistance (AMR).

https://ijvm.ut.ac.ir/article_98900_830366a46fe833399d24368cc3a7559d.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Serum Trace Elements and Oxidant/Antioxidant Status in Persian Cats With Dermatophytosis Compared to Other Dermatological Disorders 307 316 96033 10.32598/ijvm.19.2.1005476 EN Bahareh Ahmadi Torkamani Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Samaneh Eidi Section of Mycology Research, Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Mohammad Heidarpour Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Javad Khoshnegah Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Journal Article 2023 11 29 Background: Despite the high prevalence of dermatophytosis in cats, little is known about the impact of this disease on the antioxidant status and trace elements in these animals. Objectives: This study aimed to investigate the concentration of serum trace elements (copper, iron, zinc, and selenium) and oxidant/antioxidant status (malondialdehyde, total antioxidant capacity (TAC), and thiol group) in Persian cats with dermatophytosis compared to healthy controls and other dermatological disorders. Methods: Three groups of cats were selected: Cats with dermatophytosis (n=13), cats with other dermatological conditions (n=6), and clinically and dermatologically healthy cats (n=6). All 25 cats were subjected to clinical and dermatological examinations, including direct microscopic examination and fungal cultures. Additionally, possible contamination with feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) were tested. Results: Microsporum canis was the only dermatophyte species isolated from the affected cats, and only two cats were infected with the FIV: One in the dermatophytosis group and one in the other skin disease group. For trace elements, we did not detect any differences between cats with dermatophytosis and healthy cats. However, copper levels were higher in other skin disease groups than healthy controls (P<0.05). Cats with dermatophytosis and other skin diseases revealed a decrease in TAC compared to healthy controls (P<0.01). Conclusion: The present study found variations in the oxidative indices in cats with dermatophytosis and other skin disorders. This result supports the hypothesis that improving antioxidant status through dietary supplementation may be beneficial in preventing and resolving skin diseases in cats.

https://ijvm.ut.ac.ir/article_96033_7b54a8830041c101fb0b5e96216d3e67.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Effect of Solanum Melongena Peel Extracts on Glucose Level and Biochemical Parameters in Alloxan-Diabetic Mice 317 332 101527 10.32598/ijvm.19.2.1005611 EN Zena Ismail Abdullah Department of Chemistry, College of Education for Pure Sciences, University of Mosul, Mosul, Iraq. Nashwan Ibrahem Al-Lehebe Department of Chemistry, College of Education for Pure Sciences, University of Mosul, Mosul, Iraq. Journal Article 2024 07 16 Background: Natural compounds are safe and commonly used in medicine. Diabetes is a chronic metabolic disease that is widely spread in the world. Natural products can decrease blood sugar levels. This effect controls the pancreas and metabolic pathways. Objectives: The aim of this research is to extract effective compounds from eggplant peels and study their effects on blood glucose levels, lipid profiles, kidney function, and liver enzymes in diabetic mice. Methods: In this study, crude extract and some natural products such as oils, polyphenols, and anthocyanins were isolated. These extracts were identified by gas chromatography (GC) and high-performance liquid chromatography (HPLC). Some parameters were measured, such as glucose, urea, creatinine, triglyceride (TG), high- and low-density lipoprotein cholesterol (LDL-c), very low-density lipoprotein cholesterol (VLDL-c), alanine transaminase, aspartate aminotransferase (AST), lipase, and atherogenic index in male diabetic mice. Results: The results showed the presence of many fatty acids, polyphenols, and anthocyanin compounds in eggplant peels. A significant effect on lipid profiles, glucose, creatinine, and lipase was found after ten days of treatment in diabetic mice with the aforementioned extracts. In contrast, a nonsignificant effect was noted in HDL-c and urea levels with oil and polyphenol extracts. Nevertheless, these extracts had no effect on AST and ALT levels. Conclusion: In this study, the effects of these extracts were found to be mixed. However, they demonstrated beneficial effects on blood glucose, creatinine levels, and lipid profiles. These extracts may help reduce the severity of diabetes or its complications. No clear effect was observed in liver function tests.

https://ijvm.ut.ac.ir/article_101527_c239b2f28ae46eba2f3be318b12a45bb.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Effects of Vitamin E on Electrocardiographic Parameters of Broiler Chickens With Pulmonary Hypertension 333 340 100659 10.32598/ijvm.19.2.1005708 EN Hamed Zarei Department of Biology, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Hamid Yaeghoobzadeh Department of Basic Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran. Leila Mohammadyar Department of Basic Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran. Journal Article 2024 12 24 Background: Pulmonary hypertension in broiler chickens is a multifactorial syndrome leading to significant mortality, primarily due to hypoxemia and cardiac overload. Objectives: This study aimed to assess the impact of vitamin E supplementation on electrocardiographic parameters in broiler chickens subjected to triiodothyronine (T3)-induced pulmonary hypertension. Methods: A total of 180 chicks were randomly assigned to four groups: One control and three treatment groups. The chicks in the treatment groups received vitamin E (α-tocopherol) at doses of 100, 200, and 400 IU from one day to 49 days of age. To induce pulmonary hypertension, all chicks were administered 1.5 mg/kg of T3 from the seventh day to the end of the experiment through their diet. Electrocardiograms were recorded at 14 and 49 days of age, and right ventricular hypertrophy (RVH) was assessed post-euthanasia. Results: Significant decreases in the right ventricle/ total ventricle weight (RV/TV) ratio were observed in the treated groups on day 49 (P<0.05). Electrocardiographic analysis revealed reductions in S, T, and R wave amplitudes specifically on day 49 for all vitamin E doses (P<0.05). The QRS interval significantly increased at all doses on day 49, while the RR interval increased at certain doses on days 14 and 49 compared to the control group (P<0.05). Conclusion: Vitamin E supplementation effectively modulates the induction of pulmonary hypertension and associated ventricular hypertrophy in broiler chickens.

https://ijvm.ut.ac.ir/article_100659_e1e841f00b3459b6dc75e62e11fbff47.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Protective Effects of Eugenol Against Iron Overload-induced Nephrotoxicity in Male Rats 341 356 97605 10.32598/ijvm.19.2.1005540 EN Ahmed M. Malik Al-Mashhadi Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary, Medicine University of Kufa, Kufa, Iraq. Nabeel Mohammed Al-Sharafi Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary, Medicine University of Kufa, Kufa, Iraq. Journal Article 2024 03 23 Background: Nephrotoxicity is a term used to describe when the renal system suffers from reduced renal function due to both direct and indirect toxin damage caused by exposure to certain drugs. Objectives: This study aimed to investigate the protective effects of eugenol against iron overload (IOL)-induced nephrotoxicity in male rats. Methods: Thirty rats were randomly divided into six equal groups: The first group, control negative C-, received intraperitoneal (IP) injection of distilled water. The second group, control positive C+, received iron dextran only at 100 mg/kg body weight (BW) IP. The third and fourth groups (iron+eugenol [IE]1 and IE2) received iron dextran 100 mg/kg BW IP and eugenol 50,100 mg/kg BW orally, respectively. The fifth and sixth groups (E3 and E4) received eugenol only at 50 100 mg/kg BW orally. Results: The results revealed significant improvements in biomarkers and histological characteristics in rats treated with eugenol compared to those in the control group (C+). Rats treated with eugenol exhibited decreased levels of creatinine, blood urea nitrogen (BUN), malondialdehyde (MDA), erythropoietin (EPO) and kidney injury molecule-1 (KIM-1), along with increased concentrations of glutathione (GSH). Microscopic examination of kidney tissue from the control (C-) and eugenol-treated (E3 and E4) groups showed typical histological features, indicating preserved kidney architecture. In contrast, the control group (C+) showed epithelial cell necrosis in the renal tubules and inflammatory processes, particularly in the glomeruli and interstitial sections of the proximal renal tubules. The (IE1 and IE2) groups exhibited varying degrees of renal damage, with IE1 showing moderate epithelial cell necrosis and inflammation, while IE2 displayed relatively normal cortical architecture with mild inflammatory changes in the medulla. Conclusion: Eugenol ameliorated IOL-induced nephrotoxicity in male rats.

https://ijvm.ut.ac.ir/article_97605_3f54be00117a18191671cfd1dd2dac27.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Effects of Betaine on Ameliorating Depression in Gonadectomized Male Rats 357 366 95509 10.32598/ijvm.19.2.1005466 EN Jina Ghasemi Jalal Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran. Shahin Hassanpour Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran. Journal Article 2023 11 08 Background: Depression is a major mental disorder categorized by mood impairment, and betaine has antinociceptive activity in mice. Objectives This study aimed to determine the antidepressive activity of betaine in gonadectomized male rats. Methods: Twenty adult male rats were allocated to four experimental groups. Group 1 was kept in the control group, and castration was performed in the other groups. Group 2 was the sham group, which was castrated without treatment. In the imipramine group, the rats were castrated and administered imipramine (15 mg/kg) for two weeks. In group 4, following castration, the rats were intraperitoneally injected with betaine (30 mg/kg) for two weeks, respectively. Antidepressive tests were performed using the forced swimming test (FST), tail suspension test (TST), and open field test (OFT). At the end of the study, blood samples were collected from each cardiac mouse, and serum malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) levels were determined. Results: Castration significantly increased the immobility time in the FST and TST and the activity of the rats in the OFT (P<0.05). Administration of the betaine with castration significantly decreased mobility time on FST and TST and the rat’s movement in OFT compared to the untreated group (P<0.05). Castration significantly increased serum MDA levels and decreased SOD, GPx, and CAT levels compared to the control group (P<0.05). Betaine significantly decreased serum MDA levels and enhanced SOD, GPx, and CAT levels compared to the control group (P<0.05). Conclusion: These results suggest that betaine, a natural antioxidant, is beneficial in decreasing castration-induced depressive behaviors.

https://ijvm.ut.ac.ir/article_95509_725aac9e09c5a1b587fd5f84f69c5f74.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Assessment of the Toxicity of Polystyrene Microplastic in the Colon and Liver of Adult NMRI Mice 367 376 96876 10.32598/ijvm.19.2.1005532 EN Somaye Zangene Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 0009-0002-5856-0329 Hassan Morovvati Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Hojat Anbara Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Nicola Bernabò Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, Teramo, Italy. Journal Article 2024 03 13 Background: In recent years, microplastics (MPs), emerging environmental contaminants measuring less than 5 mm in diameter, have garnered significant attention. Objectives: This study aims to evaluate the impact of MPs on colon samples, which are directly exposed to MPs that enter the digestive tract through food, and on the liver, which is responsible for processing chemicals from the digestive tract in mice. Methods: During this experiment, 36 adult male mice were randomly divided into four groups of nine animals each. Three groups received polystyrene MPs (PS-MPs) at doses of 0.001, 0.01, and 1 (gavage) for 42 days; a control group was also considered. Tissue samples were collected for histomorphological, histomorphometric, inflammatory factor, and gene expression analyses 24 h after the last treatment. Results: The results showed that receiving PS-MPs negatively affected on the histomorphology and histomorphometry of the colon and liver. Also, PS-MPs caused a significant increase (P<0.05) in inflammatory factors, such as tumor necrosis factor α (TNF-α) and prostaglandin E2 (PGE2), compared to the control group. In addition, a significant increase (P<0.05) in β-catenin and hypoxia-inducible factor 1-alpha (HIF-1α) messenger ribonucleic acid (mRNA) expression was observed in the groups treated with PS-MPs compared to the control group. Conclusion: PS-MPs negatively affect histomorphology and histomorphometry and increase the concentration of TNF-α and PGE2 and the expression of HIF-1α and β-catenin genes in the colon.

https://ijvm.ut.ac.ir/article_96876_2db864d78f475689d06b3caff6c462b8.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Protective Effect of Propolis and Vitamin E on Testicular Dysfunction in Male Rats Induced by Cadmium Chloride 377 384 101509 10.32598/ijvm.19.2.1005656 EN Ahzan K. Abdulameer Department of Clinical Science, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq Saif Sattar Rasheed Department of Pathology and Poultry diseases, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq Fouad Zeidan Hamza Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq Karar Kahya Sigma Veterinary Laboratory, Najaf, Iraq Journal Article 2024 10 15 Background: Testicular dysfunction in male rats can manifest in various ways and may be caused by genetic, environmental, hormonal, and nutritional factors. Objectives: This study aims to evaluate the effects of Propolis and vitamin E against induced testicular dysfunction by cadmium chloride (CdCl2) in male rats. Methods: Forty adult male rats were randomly divided into four groups containing 10 animals each as follows: Group 1 (negative control group): rats received distilled water; group 2 (positive control group): Rats received CdCl2 at 1 mg/kg every 72 h via intraperitoneal (IP) injection; group 3 (CdCl2+propolis): rats were treated with Propolis at 250 mg/kg body weight (BW) orally daily by a gavage needle, followed by CdCl2 at 1 mg/kg IP injection every 72 h and group 4 (CdCl2 + Vit E): rats were treated with Vit E at 100 mg/kg body weight (BW) orally daily by a gavage needle followed by CdCl2 1 mg/kg by IP injection every 72 h. After four weeks, the animals were sacrificed, and tissues were collected to measure experimental parameters, including histopathological study, testosterone level, tumor necrosis factor α (TNF-α), and caspase 3. Results: The results showed a significant (P≤0.5) increase in caspase and TNF-α in G2 compared to G1, while testosterone showed a significant (P≤0.5) decrease in G2 compared to G1. Treatment with propolis and vitamin E enhanced testosterone, TNF-α, and caspase three concentrations compared with G2 (positive group). Histopathological analysis of Propolis and Vitamin E restored normal tissue structure compared to the positive control group, showing clear damage in the interstitial Leydig cells and severe damage in the interstitial connective tissue. In contrast, the Sertoli cells and primary spermatocytes showed clear necrotic changes. Conclusion: The study concluded that propolis and vitamin E have neuroprotective effects against testicular dysfunction.

https://ijvm.ut.ac.ir/article_101509_cfa5a33d1fbf510cbd071274dddd8c83.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Comparative Evaluation of Disinfectants’ Efficacy in Reducing Bacterial and Fungal Contamination in Livestock Feed Production 385 396 95717 10.32598/ijvm.19.2.1005480 EN ‌Arash Omidi Department of Animal Health Management, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran. Hassan Mosleh Department of Food hygiene and Quality Control, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran. Maryam Rahimi-Kakolaki Department of Food hygiene and Quality Control, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran. Journal Article 2023 12 03 Background: Disinfectants in feed factories are crucial in maintaining a clean and hygienic environment, preventing disease spread, controlling cross-contamination, and ensuring product quality, thereby ensuring food safety. Objectives: This study aims to assess the performance of multiple disinfectants in a factory producing livestock, poultry, and aquatic feed, as well as in a laboratory. Methods: Microplate and agar-well diffusion methods were utilized to assess the efficiency of commercial chemical disinfectants (1 and 2) and formalin (37%) on the internal surfaces of the mixer, mill, extruder, dryer, and cooler in the factory and to examine the performance of eight common disinfectants, including disinfectants 1, 2, and 3, sodium hypochlorite (NaClO) (10%), ethanol (70%), methanol (70%), povidone-iodine (10%), and formalin, against Salmonella typhimurium, Escherichia coli, and Fusarium oxysporum. Results: The extruder had the highest level of microbial contamination, while the cooler had the lowest level. Disinfectant 2 and formalin showed the most effective antibacterial and antifungal properties. Disinfectants 2 and 3 showed the highest antibacterial effects in the laboratory, while other disinfectants had the lowest. Disinfectant 2 showed the strongest antifungal effect, followed by formalin, povidone-iodine, and NaClO. Ethanol and methanol showed the least effect. Conclusion: The study emphasizes the importance of selecting effective disinfectants to reduce contamination in animal feed production facilities. Disinfectant 2 (Huwa-San TR-50), with its unique combination of hydrogen peroxide and silver-based ionic chemistry, is a powerful disinfectant solution for various applications. These results can serve as a valuable guide for choosing appropriate disinfectants for similar industries.

https://ijvm.ut.ac.ir/article_95717_51f4971cb2dac94ba5141e33b660b30c.pdf

University of Tehran Iranian Journal of Veterinary Medicine 2251-8894 19 2 2025 04 01 Successful Treatment of Feline Infectious Peritonitis Disease (FIP) with Mesenchymal Stem Cells 397 404 98910 10.32598/ijvm.19.2.1005555 EN Samira Mohamadian Department of Internal Medicine, Pouya Pet Clinic, Tehran, Iran. Pejman Kazerooni Department of Internal Medicine, Pouya Pet Clinic, Tehran, Iran. Ali Taheri Mirghaed Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Mahsa Kia Department of Stem Cell Research Technical, Treata Caspian Parsian Company, Tehran, Iran. Minoo Soltani Rastegar Reference Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Ali Akbaripazouki Department of Cell and Molecular Biology, Faculty of Biology, University of Tehran, Tehran, Iran. Saman Ahani Department of Diagnostic Imaging, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran. Pouria Asgari Department of Clinical Science, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran. Journal Article 2024 04 17 Feline infectious peritonitis (FIP) caused by feline coronavirus (FCoV) is a common disease that leads to a cytokine storm and causes organ failure, with a high mortality rate in feline patients. This is the first case report on the detailed treatment of three cats with FIPs using allogeneic bone marrow mesenchymal stem cells. We aimed to evaluate the effectiveness of cell therapy in this disease in a shorter period with greater efficiency. Infected cats received five doses of bone marrow stem cells through intravenous infusion. During the treatment period, the subjects were kept in an isolated place and their clinical conditions were evaluated under the supervision of an internal specialist. This treatment resulted in the full recovery of all cats within 21 days. One cat was re-infected two months later after exposure to an infected cat, while two cats remained in remission at the time of writing this report. This case report suggests the effectiveness of using bone marrow mesenchymal stem cell therapy in the treatment of FIPs.

https://ijvm.ut.ac.ir/article_98910_42e38ed5a8572efdb11ee465a204c117.pdf