Background: The structural health of the testes and semen quality in rams depend on their ability to withstand oxidative damage and nutritional imbalances. Although research has shown that Withania somnifera and vitamin E provide benefits, their specific effects on male reproductive health and their ability to reduce oxidative stress have not been determined.
Objectives: This research examined the effects of an aqueous extract of W. somnifera administered with vitamin E supplements on testicular dimensions and semen characteristics in rams.
Methods: The research included 24 clinically healthy rams that were ten months old and weighed 35±0.5 kg. The animals were divided into four groups of six each. The T1 animals received only the basal ration, the T2 animals received W. somnifera at 150 mg/kg body weight, and the T3 animals received vitamin E at 100 mg/kg body weight. The T4 animals received both agents simultaneously. The study lasted 12 weeks, during which the animals received their assigned treatments orally. The researchers measured testicular dimensions twice a week and collected semen samples to analyze volume, concentration, sperm motility, and morphology.
Results: The T4 group, which received both supplements, demonstrated substantial improvements in sperm motility, ejaculate concentration, and testicular size (P≤0.05). The combined application of antioxidant and phytochemical compounds produced enhanced results, suggesting that these compounds interact with biological systems at the cellular or physiological levels.
Conclusion: The combination of W. somnifera and vitamin E resulted in major improvements in testicular structure and semen quality, showing promise for enhancing reproduction in rams. The research shows that using botanical antioxidants together with micronutrient supplements is an effective method for improving fertility and reproductive outcomes in livestock.