Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs)

Rezaei, Mahdieh; Jamshidi, Shahram; Saffarpour, Anna; Ashouri, Mahdi; Sharifi, Davood; Zamankhan Malayeri, Hamed; Tamimi, Naqa; Rokn, Amir Reza

doi:10.22059/ijvm.2013.35970

Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs)

Authors

Mahdieh Rezaei ¹

Shahram Jamshidi ¹

Anna Saffarpour ²

Mahdi Ashouri ³

Davood Sharifi ⁴

Hamed Zamankhan Malayeri ¹

Naqa Tamimi ¹

Amir Reza Rokn ⁵

¹ Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

² Department of Periodontology, International Campus School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

³ Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University of Medical Sciences, Tehran, Iran

⁴ Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

⁵ Dental Implant Research Center and Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

10.22059/ijvm.2013.35970

Abstract

BACKGROUND: Stem cell therapy in small animal medicine
is still in its infancy and few in vitro and in vivo research projects
regarding animal Mesenchymal Stem Cells (MSCs) have been
carried out. On the other hand, Cell tracking is the first step of the
cell-based therapies and is essential to recognize cell fate post
transplantation. OBJECTIVES: The aim of this study was to
isolate, characterize, and transduce cBM-MSCs. METHODS:
Canine Bone Marrow-derived Mesenchymal Stem Cells (cBMMSCs)
were isolated from bone marrow of dogs and
characterized based on morphology, differentiation capacities,
and surface marker expressions. For the first time, we labeled
cBM-MSCs by GFP-encoding lentiviral vector to track them.
RESULTS: cBM-MSCs were successfully isolated and proliferated.
Morphologically, these cells were similar to other
MSCs from other sources and species and were able to
differentiate into osteocytes and adipocytes. cBM-MSCs
expressed surface marker CD44 but were not able to express
CD34. Approximately, 70% of cells efficaciously expressed
GFPafter labeling; CONCLUSIONS:We found that GFP labeling
is an easy and effective technique to track transplanted cBMMSCs.
Our results also provide fundamental information about
canine BM-MSCs in order to use in veterinary medicine.

Keywords

canine Bone Marrow-derived Mesenchymal Stem Cells (cBMMSCs)

Characterization

green fluorescent protein (GFP)

transduction