BACKGROUND:Cystitis and pyelonephnitis in ruminants most
often result from ascending urinary tract infection (UTI).
OBJECTIVES: Investigating the prevalence of urine infection and
its relationship with histopathological findings of bladder and
kidney. METHODS: This study was carried out on 353 slaughtered
buffalos (143 female and 210 male) at Ahvaz abattoir, southwest of
Iran. After slaughter, urinary samples were taken directly from
bladder by sterile syringe. The bladder and kidney were inspected
and samples from them were taken for histophatological study. All
of the urine samples were incubated for isolation of bacteria on
blood agar and MacConkey. Biochemical characterizations of the
isolates were performed according to Bergey's manual of systemic
bacteriology. Histopathological samples were taken from
representative parts of each bladder and kidney and were stained
with haematoxylin and eosin. RESULTS: Out of the 353 urine
samples, 19 (5.38%) were positive. The percentages of infected
cattle in female and male were 6.3% and 4.8%, respectively. These
bacteria were isolated: E.coli (21%), Staphylococcus spp. (31.6%),
Streptococcus spp. (15.8%), Proteus spp. (15.8%), Klebciella spp.
(5.3%), Yercinia spp. (5.3%), and Actinimyces and Pasteurelle spp.
(5.3%). In histopathological examination, 51(14.4%) and 5 (1.4%)
of the buffalos had chronic cystitis and pyelonephritis, respectively.
Bacteria were isolated only in 3 cases of chronic cystitis and 2 cases
of pyelonephritis. Statistical analysis showed there was no
relationship between isolation of bacteria from urine with cystitis
and pyelonephritis. CONCLUSIONS:Some cases of chronic cystitis
are not able to isolate of bacteria from urine and for causing
pyelonephritis they need predisposing factors which caused urine
retention.