The effect of all-trans retinol on in vitro mouse embryo's developmental competence

Authors

1 Department of Animal Sciences, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran

2 Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University, Varamin Branch, Varamin, Iran

3 Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University, Science and research Branch, Tehran, Iran

4 Department of Statistic, Faculty of Mathematics, University of Tehran, Tehran, Iran

Abstract

BACKGROUND: All-trans retinol is a biological antioxidant
scavenging the ROS in the cell culture. OBJECTIVES: This study
was conducted to investigate the effect of all-trans retinol in
fertilization and culture medium on mouse embryo's developmental
competence. METHODS: This study was designed into two
experiments. In the first experiment, in vitro mature oocytes were
co-cultured with sperm in fertilization medium containing different
concentrations of all-trans retinol (0, 1, 5, and 10 μM). After
fertilization, zygotes in each group were separately cultured in CZB
culture medium for 5 days to the blastocyst stage. In the second
experiment, in vitro produced zygotes were cultured in CZB culture
medium containing different concentrations of all-trans retinol (0,
1, 5, and 10 μM) for 5 days to the blastocyst stage. RESULTS: In the
first experiment, the blastocyst formation rate significantly
increased by 5 μM in all-trans retinol, which was more than those of
the other groups. Also, percentage of grade one embryos was
significantly higher in the presence of 5 μM all-trans retinol than
those in the presence of 0 and 1 μM all-trans retinol. In the second
experiment, different concentrations of all-trans retinol could not
alter blastocyst formation rate; however, the percentage of grade
one embryo was higher in the presence of 10 μM all-trans retinol
than that of the control group. CONCLUSIONS: These results
showed that supplementation of fertilization medium with 5 μM alltrans
retinol could improve mouse embryo's development and
morphology. On the other hand, supplementation of embryo culture
medium can improve mouse embryo morphology without any
effect on embryo developmental competence.

Keywords

Iranian Journal of Veterinary Medicine
Volume 8, Issue 3 - Serial Number 3
October 2014
Pages 207-212

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