The effect of pure phenol on sperm parameters and fertility rate in male mice

Document Type : Pharmacology

Authors

1 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Anatomy, Tehran Medical Branch, Islamic Azad University, Tehran, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

4 Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Background: Phenol is an estrogenic and toxic compound and people are widely exposed to it, in different ways.  OBJECTIVES: The aim of this study was to determine  the  negative effects of phenol on the fertility of male mice, by investigating sperm parameters including viability, motility, fertility rates and daily sperm production (DSP). METHODS: A total of 106 adult NIH mice were divided into four groups, one control and three experimental groups (n=13). The treatment groups were given daily dose of phenol for 35 days through gavage method (30, 75 and 100 mg/kg) while the control group received only normal saline. At day 36, six mice were sacrificed from each group. Gonadosomatic index (GSI), viability, motility and daily sperm production were determined carefully. The remaining 7 mice from each group were used to mate with 2 female mice. On GD10, the female mice were sacrificed and the fertility was verified. RESULTS: Daily sperm production in treatment groups decreased significantly compared to the control group (p≤0.05). Body weight, sperm motility, viability and fertility percentage were significantly decreased in 75 and 100 mg/kg groups in comparison with the control group (p≤0.05). While the testes showed no significant changes in weight in any groups. Gonadosomatic index in the treatment groups compared to the control group, was significantly decreased (p≤0.05). CONCLUSIONS: Pure phenol could reduce fertility rate through decreasing motility, daily sperm production and sperm viability.

Keywords

Article Title [Persian]

اثر فنل خالص بر پارامترهای اسپرم و میزان باروری در موش سوری نر

Authors [Persian]

  • زهرا طوطیان 1
  • سیمین فاضلی پور 2
  • نادر گودرزی 3
  • حسینعلی عرب 4
1 گروه علوم پایه، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
2 گروه آناتومی، دانشکده پزشکی ، دانشگاه آزاد اسلامی واحد تهران ، تهران-ایران
3 گروه علوم پایه، دانشکده دامپزشکی دانشگاه رازی، کرمانشاه- ایران
4 4) گروه فارماکولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران- ایران
Abstract [Persian]

زمینه مطالعه:  فنل ترکیبی استروژنیک و سمی است که اغلب افراد به طرق مختلف و به میزان وسیعی در معرض آن هستند. هدف: هدف از این تحقیق تعیین اثرات منفی فنل بر باروری موش سوری نر از طریق بررسی پارامترهای تحرک، قدرت زنده ماندن و تولید روزانه اسپرم می‌باشد. روش کار: 106 موش سوری نژاد NIH به سه گروه تیمار و یک گروه کنترل 13 تایی تقسیم شدند. گروه‌‌های تیمار 35روز روزانه با دوزهای mg/kg‌30 ،75و 100 گاواژ شدند و گروه کنترل نرمال سالین دریافت کرد. در روز 36، 6 موش نر از هر گروه آسان کشی شده و ایندکس گونادوسوماتیک، قدرت زنده ماندن، تحرک، و تولید روزانه اسپرم به دقت تعیین گردید. 7 موش باقیمانده از هر گروه، هر کدام با دو موش ماده جفت شدند. در روز دهم آبستی،موش‌ها بیهوش و میزان باروری تعیین شد. نتایج: تولید روزانه اسپرم در گروه‌های تیمار نسبت به گروه کنترل کاهش معنی‌داری داشت (0.05>p). وزن بدن، تحرک، قدرت زنده ماندن و درصد باروری در بین گرو‌های 75، 100 با گروه کنترل دارای کاهش معنی‌داری بود (0.05>p). در حالیکه تغییرات وزن بیضه در هیچ یک از گروه‌ها معنی‌دار نبود. ایندکس گونادوسوماتیک در گروه‌های تیمار نسبت به گروه کنترل افزایش معنی‌داری داشت (0.05>p). نتیجه‌گیری‌نهایی: فنل خالص با کاهش تحرک و تولید روزانه و قدرت زنده ماندن اسپرم می‌تواند میزان باروری را کاهش دهد.

Keywords [Persian]

  • باروری
  • فنل
  • موش سوری
  • اسپرم

References

Amann, R.P. (1970) Quantitative testicular  histology and comparisons between daily sperm production as determined histologically and daily sperm output. Fertil Steril. 21: 662-672.
Baj, Z., Majewska, E., Zeman, K., Pokoca, L., Dworniak, D., Paradowski, M., Tchórzewski, H. (1994) The effect of chronic exposure to formaldehyde, phenol and organic chlorohydrocarbons on peripheral blood cells and the immune system in humans. J Investig Allergol Clin Immunol 4: 186–191.
Ban, Y., Komatsu, T., Kemi, M., Inagaki, S., Nakatsuka, T., Matsumoto, H. (1995) Testicular spermatid and epididymal sperm head counts as an indicator for reproductive toxicity in rats. Exp Anim. 44: 315-322.
Bian, Q., Qian, J., Xu, L.C., Chen, J.F., Song, L., Wang, X.R. (2006) The toxic effects of 4-tert- octylphenol on the reproductive system of male rats. Food Chem Toxicol. 44: 1355-1361.
Blom, E.A. (1950) One-minute live-dead sperm stain by means of eosin-nigrosin. J Fertile Steril. 1: 176-177.
Boockfor, FR., Blake, C.A. (1997) Chronic administration of 4-tert-octylphenol to adult male rats causes shrinkage of the testes and male accessory sex organs, disrupts spermatogenesis, and increases the incidence of sperm deformities. Biol Reprod. 57: 267-77.
Bruce, R.M., Santodonato, J., Neal, M.W. (1987) Summary review of the health effects associated with phenol. Toxicol Int Health. 3: 535–568.
Cosentino, M.J., Pakyz, R.E., Fried, J. (1990) Pyrimethamine: an approach to the development of a male contraceptive. Proc Natl Acad Sci. 87: 1431-1435.
Darmani, H., Al-Hiyasat, A.S. (2004) Reproductive toxic effect of bisphenol A dimethacrylate in mice. J Biomed Mater Res. 69: 637-643.
El-Dakdoky, M., Helal, M.A.M. (2007) Reproductive toxicity of male mice after exposure to nonylphenol. Bull Environ Toxicol. 79: 188-191.
Fazelipour, S., Kiaei, S., Tootian, Z. (2010) Adverse effect of heroin hydrochloride on selected male reproductive parameters in mice. Comp Clin Pathol. 19: 565-569.
Fessehaye, Y., Komen, H., Rezk, M.A., Johan, A.M., Van Arendonk, JAM., Bovenhuis, H. (2007) Effects of inbreeding on survival, body weight and fluctuating asymmetry (FA) in Nile tilapia, Oreochromis niloticus. J Aquac. 264: 27-35.
Finkelstein, Y., Rezvani, M., Garcia-Bournissen, F., Nurmohamed, L. (2007) Inactive pharmaceutical ingredients: implications for pregnancy. Can J Clin Pharmacol. 14: 17–28.
Griswold, M.D. (1995) Interactions between germ cells and sertoli cells in the testis. Biol Reprod. 52: 211-216.
Han, X.D., Tu, ZG., Gong, Y., Shen, S.N., Wang, X.Y., Kang, L.N., Hou, Y.Y., Chen, J.X. (2004) The toxic effects of nonylphenol  on the reproductive system of male rats. Reprod Toxicol 19: 215-221.
Hess, R.A., Bunick, D., Bahr, J. (2001) Estrogen, its receptors and function in the male reproductive tract, a review. Mol Cell Endocrinol. 178: 29-38. 
Hsieh, G.C., Sharma, R.P., Parker, R.D. (1988) Subclinical effect of groundwater contaminants. I. Alternation of humeral and cellular immunity by benzene in CD-I mice. Arch Environ Contam Toxicol 17: 151.
Louei Monfared, A., Salati, A.P. (2012) Hematological alterations induced by phenol exposure in Oncorhynchus mykiss. Comp Clin Pathol. 23: 529–534.
McGeady, TA., Quinn, P.J., Fitzpatrick, E.S., Ryan, M.T. (2006) Veterinary Embryology. (1st ed.) Blackwell, London, UK.
Meehan, T., Schlatt, S., O’Bryan, M.K., de Kretser,  D.M., Loveland, K.L. (2000) Regulation of germ cell and sertoli cell development by activin, follistatin, and FSH. Dev Biol. 220: 225-237.
Oberländer, G., Yeung, C.H., Cooper, T.G. (1994) Induction of reversible infertility in male rats by oral ornidazole and its effects on sperm motility and epididymal secretions. J Reprod Fertil. 100: 551-559.
Qiu, Y.L., Wu, D.S., Zeng, X.G., Zhang, H. (2005) Adverse effects of nonylphenol on the reproductive development of F1 male SD rats in sexual maturation period. Sichuan Da Xue Xue Bao Yi Xue Ban. 36: 382-385.
Robert, B.R.W., Anderson, R.A. Jr., Oswald, C., Zaneveld, L.J.D. (1983) Ethanol induced male reproductive tract pathology as a function of ethanol dose and duration of exposure. J Pharmacol Exp Therap. 225: 470-478.
Salian, S., Doshi, T., Vanage, G. (2009) Neonatal exposure of male rats to Bisphenol A impairs fertility and expression of sertoli cell junctional proteins in the testis. Toxicology. 265: 56-67.
Takahashi, O., Oishi, S. (2003) Testicular toxicity of dietarily or parenterally administered bisphenol A in rats and mice. Food Chem Toxicol. 41: 1035-1044.
Tan, B.L.L., Kassim, N.M., Mohd, M.A. (2003) Assessment of pubertal development in juvenile male rats after sub-acute exposure to bisphenol A and nonylphenol. Toxicol Lett. 143: 261-270.
Tootian, Z., Louei Monfared, A., Fazelipour, S., Sheibani, M.T., Rouholla, F., Sasani, F., Molaemi, E. (2012) Biochemical and structural changes of the kidney in mice exposed to phenol. Turk J Med Sci. 42: 695–703.
Toth, G.P., Wang, S.R., McCarthy, H., Tocco, D.R., Smith, M.K. (1992) Effects of three male reproductive toxicants on rat cauda epididymal sperm motion. Reprod Toxicol. 6: 507-515.
Vom Saal, FS., Cooke, P.S., Buchanan, D.L., Palanza, P., Thayer, K.A., Nagel, S.C., Parmigiani, S., Welshons, W.V. (1998) A physiologically based approach to the study of bisphenol A and other estrogenic chemicals on the size of reproductive organs, daily sperm production, and behavior. Toxicol Ind Health. 14: 239-60.
Windus-Podehl, G., Lyftogt, C., Zieve, L., Brunner, G.J. (1983) Encephalopathic effect of phenol in rats. Lab Clin Med. 101: 586–592.
 
Iranian Journal of Veterinary Medicine
Volume 9, Issue 4 - Serial Number 4
January 2016
Pages 295-301

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