Frequency of selected virulence-associated genes in intestinal and extra-intestinal Escherichia coli isolates from chicken

Document Type : Infectious agents- Diseases

Authors

1 DVSc (Microbiology) Student, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Molecular Microbiology Research Group, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran

4 Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

5 DVSc (Poultry Diseases) Graduate, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Background: Although Escherichia coli (E. coli)
is a part of intestinal normal microflora of warm-blooded animals, including
poultry, outbreaks occur in poultry raised below standard sanitation and during
the course of respiratory or immunosuppressive diseases. Avian pathogenic E.
coli (APEC) harbors several genes associated with virulence and
pathogenicity. APEC strains are responsible for some diseases in poultry
including colibacillosis, swollen head syndrome, yolk sac infection, omphalitis
and coli granuloma. OBJECTIVES:  The
aim of this study was examination of the presence and frequency of three
important virulence genes in intestinal and extra-intestinal (liver) E. coli
isolates from chicken of Khuzestan province in the southwest of Iran. METHODS:
Totally 120 (60 intestinal and 60 liver) E. coli isolates were examined
by polymerase chain reaction (PCR) for the presence of aerobactin (iutA),
temperature sensitive hemagglutinin (tsh) and fimbriae type 1 (fimH )
genes.  RESULTS: The results showed
that tsh, iutA and fimH are respectively present in 78.3%, 70% and 61.7% of
liver isolates while in intestinal ones the frequency of these genes was 21.7%,
41.7% and 41.7% respectively. The most prevalent genotypes in extra intestinal
and intestinal isolates were tsh+fimH+iutA+ and tsh-fimH-iutA-respectively. CONCLUSIONS: It seems that these sets of virulence genes are significantly more
prevalent (P<0.05) in extra intestinal isolates and probably these genes
play an important role in the pathogenesis of APEC isolates in the southwest of
Iran. Although these virulence genes were not present in all APEC isolates  their frequencies were high and using the
products of these genes in vaccines may be effective in protecting against
infections caused by this bacterium

Keywords

References

Brzuszkiewicz, E., Thürmer, A., Schuldes, J., Leimbach, A., Liesegang, H., Meyer, F.D., Boelter, J., Petersen, H., Gottschalk, G., Daniel, R. (2011) Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: entero-aggregative-haemorrhagic Escherichia coli (EAHEC). Arch Microbiol. 193: 883-91.
Campos, T.A., Stehling, E.G., Ferreira, A., Castro A.F.P., Brocchi, M., Silveria, W.D. (2005)   Adhesion properties, fimberial expression and PCR detection of adhesion related genes of avian Escherichia coli strains. Vet Microbiol. 106: 275-285.
Cavicchio, L., Dotto, G., Giacomelli, M., Giovanardi, D., Grilli G., Franciosini, M.P., Trocino, A., Piccirillo, A. (2015) Class 1 and class 2 integrons in avian pathogenic Escherichia coli from poultry in Italy.  Poult Sci. 3: 1–7.
Delicato, E.R., Guimaraes de Brito, B., Gaziri, L.C.J., Vidotto, M.C. (2003) Virulence associated genes in Escherichia coli isolates from poultry with colibacillosis. Vet Microbiol. 94: 97-103.
Dizois, C.M., Daigle, F., Curtiss, R. (2003) Identification of pathogen specific and conserved genes expressed invivo by an avian pathogenic Escherichia coli strain. Proc Nati Acad Sci USA. 100: 247-252.
Ghanbarpour, R., Sami, M., Salehi, M., Ouromiei, M. (2011) Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran. Trop Anim Health Prod. 43:153-7.
Kafshdouzan, K.H., Zahraei Salehi, T., Nayeri Fasaei, B., Madadgar, O., Yamasaki, S.H., Inenoya, A., Yasuda, N. (2013) Distribution of virulence associated genes in isolated Escherichia coli from avian colibacillosis. Iran J Vet Med. 7: 1-6.
Kheirandish, R., Salehi, M., Ghanbarpour, R., Alidadi, S., Askari, N. (2012) Coligranuloma in a pigeon. Eurasian J Vet Sci. 28: 237-239.
La Ragion, R.M., Woodward, M.J. (2002) Virulence factor of Escherichia coli serotypes associated with avian colisepticaemia. Res Vet Sci. 73: 27-35.
Markey, B.K., Leonard, F.C., Archambault, M., Cullinane, A., Maguire, D. (2013) Clinical Veterinary Microbiology. (2nd ed.) Mosby. China.Maurer, J.J., Brown, T.P., Steffens, W.L., Thayer, S.G. (1998) The occurrence of ambient temperature-regulated adhesins, curli, and the temperature-sensitive hemagglutinin Tsh among avian Escherichia coli. Avian Dis. 42: 106–118.
McPeake, S.J., Smyth, J.A., Ball, H.J. (2005) Characterization of avian pathogenic Escherichia coli (APEC) associated with colisepticaemia compared to faecal isolates from healthy birds. Vet Microbiol. 110: 245-253.
Nakazato, G., Campos, T.A., Stehling, E.G., Brocchi, M., Silveria, W. (2009) Virulence factor of avian pathogenic Escherichia coli (APEC). Pesq Vet Bras. 29: 479-486.
Ramirez, R.M., Almanza, Y., Garcia, S., Heredia, N. (2009) Adherence and invasion of avian pathogenic Escherichia coli to avian tracheal epithelial cells. World J Microbiol Biotechnol. 25: 1019-1023.
Rodriguez-siek, K.E., Giddings, C.W., Doetkott, C., Johnson, T.J., Nolan, L.K. (2005) Characterizing the APEC pathotype. Vet Res. 36: 241-256.
Roussan, D.A., Zakaria, H., Khawaldeh, G., Shaheen, I. (2014) Differentiation of avian pathogenic Escherichia coli strains from broiler chickens by multiplex polymerase chain reaction (PCR) and random amplified polymorphic (RAPD) DNA. Open J Vet Med. 4: 211-219.
Salehi, M. (2014) Determination of intimin and Shiga toxin genes in Escherichia coli isolates from gastrointestinal contents of healthy broiler chickens in Kerman City, Iran. Comp Clin Pathol. 23: 125-129.
Schouler, M., Reperant, M., Laurent, S., Bree, A., Grasteau, S.M., Germon, P., Rasschaert, D., Schouler, C. (2007) Experimental pathogenic Escherichia coli strains of avian and human origin: link between phylogenetic relationships and common virulence patterns. J Clin Microbiol.  45: 3366-3376.
Wang, S., Bao, Y., Meng, Q., Xia, Y., Zhao, Y., Wang, Y., Tang, F., ZhuGe, X., Yu, S., Han, X., Dai, J., Lu, C. (2015) IbeR facilitates stress-resistance, invasion and pathogenicity of avian pathogenic Escherichia coli. PLoS One. doi: 10.1371.
Wang, S., Dai, J., Meng, Q., Han, X., Han, Y., Zhao, Y., Yang, D., Ding, C., Yu, S. (2014) DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli. Front Microbiol.  doi: 10.3389.
Iranian Journal of Veterinary Medicine
Volume 10, Issue 2
April 2016
Pages 91-96

Files

Share

How to cite

Statistics