Document Type : Nutrition - Hygiene
Authors
1 Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran,
2 Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abstract
Keywords
Article Title [Persian]
Authors [Persian]
زمینه مطالعه: کبد و قلب دو عضو اصلی آسیب دیده در سندرم آسیت در جوجه‌های گوشتی است. استفاده از سیلی‌مارین با اثر محافظتی بر کبد و قلب ممکن است یک راهکار مفید برای کاهش مرگ و میر ناشی از آسیت باشد.
هدف: مطالعه حاضر با بررسی شاخص‌های ECG و برخی از پارامترهای بیوشیمیایی سرم، اثرات قلبی-کبدی سیلی‌مارین را در جوجه‌های گوشتی تغذیه شده با رژیم غذایی آردی و پلت ارزیابی کرد.
روش کار: تعداد 120 قطعه جوجه نژاد آربراکرز به شش گروه تقسیم شدند: رژیم غذایی پایه آردی (CM)، رژیم غذایی پایه پلت (CP)، سیلی‌مارین در دوز ppm 500 جیره‌های آردی (M500) و پلت (P500)، سیلی‌مارین در دوز ppm2500 جیره‌های آردی (M2500) و پلت (P2500).
نتایج: CP فعالیت سرمی بالاتری از آنزیم‌های آسپارتات آمینو ترانسفراز، آلانین امینوترانسفراز، و کراتین کیناز در مقایسه با CM داشت (05/0p < /em><). P2500 پروتئین کل بالاتر و آسپارتات آمینو ترانسفراز، آلانین امینوترانسفراز، و کراتین پایین‌تری نسبت به CP داشت (05/0p < /em><). مدت زمان T، قطعه ST و فاصله R-R به‌طور معنیدار در CP در مقایسه با CM بیشتر و در P2500کوتاه تر از CP و P500 بود (05/0p < /em> <).
نتیجه‌گیری نهایی: جیره پلت در جوجه‌های گوشتی منجر به تغییر برخی از شاخص‌های بیوشیمیایی و الکتروکاردیوگرافی می‌شود و سیلی‌مارین با دوزppm 2500 می‌تواند به‌عنوان یک ترکیب محافظت‌کنندۀ کبد و قلب جهت تعدیل نارسایی قلبی- کبدی در جوجه‌های گوشتی حساس استفاده شود.
Keywords [Persian]
The genetic selection has led to a fast growth rate in modern strains of broiler chickens (Ahmadipour et al., 2015). In fast-growing broilers, the metabolic rate and the requirement of oxygen are increased, and the heart and lungs are not capable of providing enough oxygen, which causes metabolic disorders (Wideman et al., 2013). Right ventricular failure (RVF) and ascites syndrome are the most prominent metabolic disorders, commonly seen in broiler chickens with a high-growth rate (Ahmadipour et al., 2019).
Diet form (pellet, mash, and crumble) is one of the most important effective factors on the incidence of RVF and ascites in broilers (Sahraei, 2014). Broilers fed on the pellet diet present higher weight gain and better feed conversion ratio compared with those fed the mash diet (Amerah et al., 2008). Several studies have stated that susceptibility to RVF and ascites was higher in birds fed pelleted diets than in birds received mash diets (Jafarnejad et al., 2010; Sahraei, 2014). The heart and liver are two major damaged organs in RVF and ascites syndrome (Jafarnejad et al., 2010). Improving the health of the heart and liver using herbal extracts is a benefit and can be a clinical strategy to control RVF lesions and decrease RVF-induced mortality. Milk thistle is one of the more common medicinal herbs with protective effects on the liver and heart.
Milk thistle (Silybum marianum L., Asteraceae) is one of the most common herbs for the treatment of liver diseases (Schrieber et al., 2008). The seed and fruit of milk thistle contain silymarin that shows a variety of pharmacological activities, as well as antioxidant, anti-inflammatory, antibacterial, and antiviral properties in many experimental and clinical studies (Koçarslan et al., 2016).
Some studies have evaluated the positive effects of silymarin extract on growth performance and hepatic disorders in broilers (Schiavone et al., 2007; Kralik et al., 2015; Hosseinian et al., 2020). Further, various researches have established that silymarin had cardioprotective activity due to its antioxidant content in laboratory animals (Rao & Viswanath, 2007; Al-Rasheed et al., 2014; Koçarslan et al., 2016). However, there is a lack of knowledge about the positive effects of silymarin on broiler’s heart health. Evaluating heart electrical activity by electrocardiogram (ECG) is a simple method to assess heart health in birds (Yogeshpriya et al., 2018).
In veterinary medicine, there are several diagnostic tools for the evaluation of cardiac abnormalities, but ECG is one of the most useful and non-invasive techniques, which gives significant information about heart rate (HR), cardiac arrhythmias, and electrical conductance abnormalities (Cushing et al., 2013; Yogeshpriya et al., 2018).
In avian medicine, ECG is a useful tool utilized to measure HR and detect cardiac arrhythmias and cardiac chamber enlargement (Sharifi et al., 2015; Hosseinian et al., 2019). Several studies have indicated that RVF and ascites syndrome induce a change in the morphology of ECG waves in broilers, and they used ECG to detect and diagnosis the ascites syndrome (Hassanpour et al., 2009; Yousefi et al., 2013).
In this study, broilers were fed with two different forms of diet (mash and pellet), and heart and liver health was evaluated by assessing some serum biochemical and ECG induces. Also, the effects of two different doses of dietary silymarin (500 and 2500 ppm) were assessed on some serum parameters and ECG. In this research, this hypothesis was assessed that pellet diet can be led to heart and liver dysfunction in broilers, and silymarin could attenuate the cardiac and hepatic damages.
The results of the present study may clarify the protective effects of silymarin on the liver and heart in fast-growing broilers, and silymarin can be used as a cardioprotective and hepatoprotective compound during the rearing period of susceptible broilers to ascites syndrome.
This experiment was performed at the Veterinary College of Shiraz University. A total of 120 1-day-old Arbor Acres broiler chicks were used for the experiment and reared for 42 days. During study, broilers were raised in environmentally controlled rooms under standard environmental conditions suggested by commercial recommendations of the chick producer company (Aviagen, 2018).
On day 14 of the experiment, birds were weighed and randomly assigned into 6 equal groups (n=20) with five replicates. The treatment groups consisted of supplementation with silymarin extract in the mash and pelleted diets as follows: the control mash group (basal mash diet [CM], fed on a mash diet without silymarin), control pellet (basal pellet diet [CP], fed on a pellet diet without silymarin), two doses of silymarin in mash feed (silymarin at 500 ppm of mash [M500] and silymarin at 2500 ppm of mash [M2500]), and two doses of silymarin in pellet feed (silymarin at 500 ppm of pellet [P500] and silymarin at 2500 ppm of pellet [P2500]).
In the present study, the basal diet was corn-soybean meal-based and formulated to meet or exceed the minimum National Research Council (NRC; 1994) Standards. The starter (0-10 days), grower (10-25 days), and finisher (25-42 days) feeds were used during the experiment. All birds were fed on a pellet diet during the first and second weeks of the experiment, and then two forms of diet (mash and pellet) with a similar composition of ingredients (see Table 1) were used from the third to sixth weeks of the experiment.
Table 1. Ingredients and composition of mixture feed (gkg-1)
|
Component |
Diets |
||
|
|
Starter |
Grower |
Finisher |
|
Ingredients, % |
|
|
|
|
Yellow Corn |
53.4 |
58.0 |
61.3 |
|
Soybean meal (44%) |
39.0 |
35.0 |
31.0 |
|
Dicalcium phosphate |
1.7 |
1.5 |
1.3 |
|
CaCO3 (38%) |
1.7 |
1.4 |
1.3 |
|
Sunflower oil |
3.0 |
3.0 |
4.0 |
|
Sodium chloride |
0.3 |
0.3 |
0.3 |
|
Methionine |
0.2 |
0.15 |
0.15 |
|
Lysine |
0.2 |
0.15 |
0.15 |
|
Premix* |
0.5 |
0.5 |
0.5 |
|
Total |
100 |
100 |
100 |
|
Nutrient levels |
|
|
|
|
ME, kcal/kg |
2900 |
3000 |
3100 |
|
Crude protein, % |
22 |
20.5 |
19 |
|
Calcium, % |
1.05 |
0.9 |
0.8 |
|
Available Phosphorus, % |
0.5 |
0.45 |
0.4 |
|
Sodium, % |
0.19 |
0.19 |
0.19 |
|
Lysine, % |
1.31 |
1.25 |
1.14 |
|
Methionine, % |
0.54 |
0.48 |
0.44 |
|
Threonine, % |
0.85 |
0.81 |
0.75 |
|
Tryptophan, % |
0.25 |
0.22 |
0.2 |
* Vitamin and mineral content per kilogram of premix: vitamin A: 3,600,000 IU; vitamin D3: 800,000 IU; vitamin E: 7,200 IU; vitamin K3: 0.8 g; vitamin B1: 0.71 g; vitamin B2: 2.64 g; vitamin B3: 3.92 g, vitamin B5: 11.88 g; vitamin B6: 1.176 g; vitamin B12: 6 mg; folic acid: 0.4 g; biotin: 40 mg; choline chloride: 100 g; selenium: 80 mg; cobalt: 100 mg; iodine: 396 mg; copper:4 g; zinc: 33.88 g; iron: 20 g; manganese: 39.68 g.
The used diets in this study was prepared in the livestock affairs of the Faculty of Veterinary Medicine, Shiraz University. Firstly, the feed was supplied based on NRC Standards for broilers. Then, a part of the feed was finely grounded, mixed, and used as a mash form in mash treatments during the entire the experiment, and the other part of the feed was pelleted in a steam pellet mill. After pelleting, the pelleted feed was dried and cooled to an average temperature of 37°C and used as a pellet form in pellet treatments during the entire the experiment. Feed and freshwater were supplied ad libitum during the entire experiment.
Silymarin powder was purchased from BarijEsans Company (Iran), containing 43.87% of silibinin and 96.76% dry matter and was added to mash and pellet diets from days 14 to 42 of the experiment. All diets contained neither anticoccidials nor any other medications.
The temperature was maintained at 32°C during the first week and reduced gradually until a constant temperature of 24°C was achieved. A 24-hour lighting schedule was used during the first week and then reduced gradually until 20 h light: 4 h darkness (20L:4D).
Ten apparently healthy birds of each group were selected, and blood sampling (5 mL/birds) was done from the brachial vein on days 21, 28, 35, and 42. Blood samples were kept at room temperature for 30 minutes, and then the clotted blood samples were centrifuged at 3000g for 10 minutes to separate sera. The clear sera were collected and stored at -20℃ until the biochemical analysis.
The hepatic parameters, including total protein, albumin, globulin, uric acid, triglyceride, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL), were measured by an automated analyzer (Alpha Classic, Sanjesh Company, Iran) using commercial clinical investigation kits (Pars Azmoon, Tehran, Iran). Moreover, the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were evaluated using a commercial kit (Pars Azmoon, Tehran, Iran).
Biochemical Assays
Calcium, magnesium, phosphorous, chloride, and creatine kinase MB (CK-MB) were measured using commercial kits (Pars Azmoon, Iran) and biochemical auto analyzer (Alpha Classic, Sanjesh Company, Iran). Sodium and potassium were determined using a flame photometer (Clinical flame photometer, Fater Company, Iran).
From each group, 7 apparently healthy chicks were randomly selected on 21, 28, 35, and 42 days, and ECGs were recorded by a single channel ECG machine (Kenz-line EKG 110, Suzuken Company, Japan). The ECG measurements were obtained from unanesthetized and restrained birds in a standing position. Before ECG recording, the chickens rested for about 5 minutes to calm down. ECG gel was applied to the skin, and then alligator clip electrodes were positioned at the base of the right and left wings and gastrocnemius muscle of the right and left limbs (Yogeshpriya et al., 2018).
All ECGs were recorded using a calibration of 10 mm/mV and a paper speed of 50 mm/sec (Reddy et al., 2016). ECGs were recorded by different leads, including I, II, III, and aVR, aVL, and aVF for every chicken. In birds, lead II is commonly used to evaluate waves' morphology in ECG (Reddy et al., 2016). For this reason, the amplitude and duration of P, T, R, and S waves, as well as the duration of QT, R-R, PR, and ST intervals, in each bird of the present experiment were measured from lead II as a standard lead (Reddy et al., 2016). Finally, HR was measured in each bird.
The statistical analysis was performed using SPSS 22 (SPSS Inc., Chicago, Ill., USA). In the present study, the data from various serum parameters and ECG indices were analyzed by 1-way analysis of variance (ANOVA). The data were presented as mean ± SE. The significance level was set as P-value<0.05.
The results of serum hepatic parameters are presented in Tables 2 and 3.
As seen in Table 2, total protein and albumin significantly decreased in CP compared with CM at some times of the experiment (P<0.05). P500 and P2500 significantly had a higher total protein compared with CP on days 28, 35, and 42. Circulating uric acid increased significantly in CP and P500 compared with CM. In this experiment, P2500 had a lower level of uric acid compared to CP and P500. The serum activity of ALT and AST enzymes in the CP group was significantly higher than in CM, and the activity levels of ALT and AST decreased significantly in P2500 compared to CP on day 42 (P<0.05) (Table 2).
Table 2. Circulating hepatic parameters (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups (mean ±SE) |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
Total protein (gr/L) |
21 |
2.43±0.071a |
2.92±0.022a |
2.13±0.040a |
2.80±0.041a |
3.12±0.080b |
2.72±0.101a |
|
28 |
3.47±0.092a |
3.35±0.073a |
2.89±0.033b |
2.25±0.072b |
3.46±0.052a |
3.70±0.081a |
|
|
35 |
2.80±0.051a |
2.94±0.072a |
2.94±0.022a |
2.59±0.074a |
3.09±0.084b |
3.01±0.017b |
|
|
42 |
3.72±0.091a |
3.98±0.020a |
3.87±0.080a |
2.28±0.038b |
3.36±0.063a |
3.92±0.043a |
|
|
Albumin (gr/L) |
21 |
1.37±0.031a |
1.34±0.040a |
1.21±0.062a |
1.42±0.035a |
1.52±0.023a |
1.42±0.044a |
|
28 |
1.40±0.064a |
1.46±0.016a |
1.39±0.074a |
1.57±0.051a |
1.19±0.065a |
1.42±0.065a |
|
|
35 |
1.37±0.087a |
1.38±0.055a |
1.39±0.020a |
1.22±0.050a |
1.28±0.066a |
1.32±0.022a |
|
|
42 |
2.43±0.034a |
2.47±0.054a |
2.44±0.020a |
1.26±0.120b |
1.36±0.074b |
1.92±0.060b |
|
|
Globulin (gr/L) |
21 |
1.08±0.170a |
1.54±0.068a |
0.97±0.114a |
1.39±0.081a |
1.68±0.123a |
1.37±0.205a |
|
28 |
1.42±0.080a |
1.74±0.125a |
1.48±0.065a |
1.68±0.112a |
1.05±0.115a |
1.60±0.144a |
|
|
35 |
1.53±0.111a |
1.61±0.164a |
1.25±0.176a |
1.69±0.142a |
1.63±0.121a |
1.68±0.135a |
|
|
42 |
1.27±0.201a |
1.58±0.097a |
1.36±0.078a |
1.40±0.163a |
1.73±0.130a |
1.39±0.084a |
|
|
Uric acid (mg/dL) |
21 |
2.83±0.225a |
3.58±0.189b |
2.85±0.197a |
3.23±0.305b |
4.60±0.404c |
3.98±0.513b |
|
28 |
3.22±0.297a |
3.95±0.348a |
3.85±0.135a |
4.51±0.434b |
2.71±0.175c |
3.80±0.305a |
|
|
35 |
3.94±0.271a |
3.92±0.313a |
4.09±0.491a |
3.72±0.300a |
4.55±0.424b |
3.71±0.294a |
|
|
42 |
3.90±0.519a |
4.21±0.190b |
3.28±0.294a |
4.26±0.455b |
4.08±0.313b |
3.20±0.315a |
|
|
Alanine aminotransferase (IU/L) |
21 |
126.84±9.110a |
122.60±9.158a |
99.45±3.780b |
125.39±8.937a |
121.44±4.844a |
128.55±4.385a |
|
28 |
127.88±7.910a |
129.29±7.856a |
110.88±6.125b |
132.79±3.809a |
129.98±4.496a |
125.01±6.147a |
|
|
35 |
128.04±9.747a |
124.55±6.144a |
97.58±3.751b |
132.88±6.568a |
119.57±4.728a |
109.29±6.446a |
|
|
42 |
97.88±5.887a |
112.45±6.783b |
109.11±5.360b |
128.19±4.018b |
130.01±3.950b |
95.48±5.010a |
|
|
Aspartate aminotransferase (IU/L) |
21 |
253.70±10.317a |
245.60±10.958a |
199.90±7.888b |
258.30±8.178a |
242.90±9.981a |
248.30±6.480a |
|
28 |
252.80±8.015a |
257.20±8.154a |
240.00±12.125b |
265.70±6.906a |
259.90±7.410a |
249.70±10.744a |
|
|
35 |
240.30±10.846a |
242.40±11.541a |
193.20±6.785b |
263.50±11.765a |
229.70±7.027a |
203.20±12.033b |
|
|
42 |
199.50±6.812a |
219.50±7.683b |
207.90±8.664a |
241.30±6.112b |
247.90±8.255b |
198.60±9.050a |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
As seen in Table 3, the serum levels of total triglyceride, cholesterol, and LDL in supplemented groups (i.e., M500, M2500, P500, and P2500) decreased significantly compared to CM and CP groups (P<0.05). The CP group had lower concentrations of VLDL and LDL compared to the CM group.
Table 3Circulating hepatic indices (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups (mean ±SE) |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
Triglyceride (mg/dL) |
21 |
85.70±1.820a |
87.70±3.285a |
62.80±1.655b |
83.30±3.850a |
77.50±1.22b |
63.00±2.35b |
|
28 |
85.70±3.180a |
71.50±1.980b |
79.00±3.996b |
72.50±1.544b |
55.80±1.96c |
66.90±1.49b |
|
|
35 |
76.40±1.431a |
64.70±1.321b |
66.20±2.027b |
84.90±1.242c |
65.40±1.65b |
63.40±3.91b |
|
|
42 |
83.80±1.262a |
73.50±1.825b |
62.40±3.221c |
89.10±2.19a |
72.50±1.65b |
60.40±3.91c |
|
|
Cholesterol (mg/dL) |
21 |
148.50±2.39a |
100.80±6.32b |
98.50±2.551b |
145.60±2.16a |
101.50±2.54b |
103.20±3.1b |
|
28 |
148.50±2.71a |
122.80±2.51b |
122.40±2.03b |
144.30±3.74a |
96.10±2.640c |
122.50±3.8b |
|
|
35 |
139.40±2.64a |
110.70±3.48b |
107.60±4.62b |
139.50±3.09a |
100.70±3.49b |
97.20±3.87b |
|
|
42 |
135.20±1.24a |
116.00±2.13b |
105.40±2.24c |
143.20±3.20a |
113.90±1.71b |
93.30±2.58c |
|
|
HDL (mg/dL) |
21 |
70.60±1.07a |
78.20±0.284a |
71.10±1.425a |
70.90±0.704a |
71.70±1.094a |
74.20±0.520a |
|
28 |
70.90±0.92a |
72.60±0.921a |
76.00±0.285a |
71.40±1.175a |
70.30±0.975a |
77.30±0.634a |
|
|
35 |
75.30±0.25a |
70.30±0.908a |
70.80±1.034a |
75.00±0.870a |
76.00±0.891a |
70.90±0.910a |
|
|
42 |
67.10±1.09a |
74.20±0.169a |
71.30±0.363a |
70.00±1.954a |
73.10±1.208a |
71.40±1.097a |
|
|
LDL (mg/dL) |
21 |
93.00±2.23a |
58.70±2.41b |
54.80±1.27b |
81.20±1.944c |
55.90±1.50b |
51.00±1.74b |
|
28 |
85.60±0.64a |
54.00±1.38b |
59.60±1.34b |
77.70±1.485a |
53.40±0.21b |
60.50±1.47b |
|
|
35 |
89.80±0.45a |
53.20±2.43b |
49.50±2.20b |
71.70±1.853c |
56.70±1.79b |
52.70±1.30b |
|
|
42 |
91.50±1.40a |
55.80±2.187c |
49.90±0.372c |
75.60±1.05b |
50.50±1.660c |
57.70±1.950c |
|
|
VLDL (mg/dL) |
21 |
17.06±0.43a |
18.13±0.588a |
12.54±0.638c |
15.52±0.95b |
15.68±0.25b |
12.65±0.594c |
|
28 |
17.19±0.67a |
14.29±0.18b |
16.58±0.780a |
14.99±0.36b |
11.05±0.966c |
13.28±0.49b |
|
|
35 |
15.24±0.48a |
12.98±0.52b |
13.24±0.45b |
16.90±0.818a |
13.80±0.95b |
12.65±0.73b |
|
|
42 |
16.80±0.36a |
14.72±0.37b |
12.40±0.676c |
17.80±0.890a |
14.54±0.190b |
12.31±0.760c |
|
|
Total billirubin (mg/dL) |
21 |
0.46±0.024c |
0.51±0.014b,c |
0.42±0.011a,c |
0.50±0.074b |
0.55±0.104b |
0.48±0.081a,c |
|
28 |
0.48±0.078b |
0.51±0.076a,b |
0.51±0.050a,b |
0.56±0.090a |
0.44±0.054c |
0.49±0.041b,c |
|
|
35 |
0.51±0.017a |
0.51±0.074a |
0.50±0.104a |
0.50±0.012a |
0.54±0.031a |
0.50±0.032a |
|
|
42 |
0.46±0.019a |
0.58±0.020b |
0.50±0.024a |
0.51±0.090a,b |
0.53±0.080a,b |
0.49±0.071a,b |
|
|
Direct billirubin (mg/dL) |
21 |
0.03±0.011a,b |
0.02±0.003b |
0.03±0.011a,b |
0.03±0.004a,b |
0.02±0.006a,b |
0.04±0.001a |
|
28 |
0.04±0.020a |
0.04±0.003a |
0.03±0.021a |
0.03±0.007a |
0.03±0.003a |
0.03±0.002a |
|
|
35 |
0.03±0.000a |
0.04±0.001a |
0.03±0.002a |
0.03±0.005a |
0.04±0.004a |
0.04±0.003a |
|
|
42 |
0.04±0.002a |
0.03±0.011a |
0.03±0.004a |
0.03±0.004a |
0.03±0.003a |
0.03±0.001a |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; HDL: high-density lipoprotein cholesterol; LDL: low-density lipoprotein cholesterol; VLDL: very low-density lipoprotein cholesterol; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
The results of serum cardiac parameters are presented in Table 4.
The circulating levels of calcium and phosphorus were significantly lower in the CP group than in other groups on 28, 35, and 42 days (P<0.05). P500 and P2500 had significantly higher concentrations of calcium and phosphorus compared with CP (P<0.05). CP had a higher activity of CK enzyme compared with CM on day 42, and P500 and P2500 had a lower serum activity of CK compared with CP (P<0.05) (Table 4).
Table 4Circulating electrolytes and cardiac parameters (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups (mean ±SE ) |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
Calcium ( mg/dL) |
21 |
5.71±0.180a |
5.09±0.354a |
3.99±0.354b |
5.28±0.091a |
6.66±1.771c |
5.7±0.721a |
|
28 |
7.28±0.020a |
7.39±0.092a |
6.94±0.101a |
5.45±0.011b |
6.88±0.082a |
7.09±0.062a |
|
|
35 |
6.31±0.116a |
6.03±0.093a |
6.57±0.230a |
4.28±0.081b |
6.36±0.482a |
6.32±0.240a |
|
|
42 |
7.79±0.064a |
7.79±0.151a |
7.05±0.192a |
5.14±0.060b |
7.22±0.033a |
7.84±0.120a |
|
|
Phosphorus (mg/dL) |
21 |
4.90±0.351a |
4.79±0.350a |
4.56±0.231a |
5.15±0.304a |
5.23±0.304a |
4.79±0.323a |
|
28 |
6.09±0.101a |
5.32±0.111a |
5.63±0.122a |
4.63±0.151b |
4.61±0.082b |
5.80±0.062a |
|
|
35 |
5.59±0.102a |
5.35±0.082a |
5.77±0.113a |
3.17±0.052b |
5.05±0.044a |
5.25±0.060a |
|
|
42 |
6.72±1.130a |
5.99±0.203a |
5.12±0.314b |
4.78±0.343b |
5.67±0.332a |
6.89±0.330a |
|
|
Sodium (mmol/L) |
21 |
125.53±3.880a |
122.33±5.540a |
129.76±5.34a |
119.98±8.981a |
111.28±3.990a |
113.98±6.184a |
|
28 |
130.45±4.431a |
129.87±7.011a |
138.65±9.89a |
112.23±6.941b |
119.63±5.844b |
115.23±6.44b |
|
|
35 |
132.98±5.211a |
132.13±8.902a |
128.51±7.09a |
119.98±7.862b |
129.47±7.012a |
142.78±6.961c |
|
|
42 |
140.89±10.09a |
132.44±5.985a |
130.87±9.89a |
123.67±9.321b |
119.17±8.330b |
143.97±7.920a |
|
|
Potassium (mg/dL) |
21 |
4.87±1.06a |
4.56±2.032a |
5.01±1.234a |
4.88±0.985a |
4.77±1.094a |
4.61±0.285a |
|
28 |
4.78±0.984a |
3.99±1.014a |
4.66±2.135a |
4.35±0.691a |
4.98±1.971a |
4.09±0.325a |
|
|
35 |
4.90±0.214a |
4.52±1.124a |
4.87±3.095a |
5.01±0.274a |
4.81±0.542a |
4.62±0.477a |
|
|
42 |
4.98±0.792a |
5.06±3.095a |
4.72±2.017a |
4.55±0.936a |
4.76±0.437a |
4.72±0.732a |
|
|
Magnesium (mg/dL) |
21 |
1.88±0.091a |
2.15±0.067a |
1.84±0.065a |
2.02±0.071a |
2.25±0.134a |
2.06±0.134a |
|
28 |
2.07±0.084a |
2.31±0.085a |
2.22±0.046a |
2.23±0.060a |
1.85±0.062a |
2.29±0.085a |
|
|
35 |
2.41±0.072a |
2.38±0.076a |
2.40±0.057a |
2.26±0.074a |
2.22±0.045a |
2.39±0.066a |
|
|
42 |
2.52±0.033a |
2.55±0.044a |
2.62±0.070a |
2.16±0.052a |
2.29±0.080a |
2.54±0.070a |
|
|
Chloride (mmol/L) |
21 |
105.30±0.225a |
103.90±0.455a |
107.50±0.445a |
105.70±0.512a |
107.50±0.664a |
106.80±0.53a |
|
28 |
106.70±0.650a |
108.70±0.346a |
105.80±0.323a |
104.60±0.380a |
110.30±0.415a |
106.30±0.47a |
|
|
35 |
107.80±0.642a |
109.40±0.854a |
103.90±0.327a |
104.50±0.514a |
108.50±0.865a |
108.30±0.44a |
|
|
42 |
104.80±0.337a |
106.20±0.473a |
105.10±0.610a |
103.80±0.515a |
107.40±0.534a |
108.30±0.49a |
|
|
CK-MB (IU/L) |
21 |
1481.80±12.890a |
1474.50±10.081a |
1235.22±13.06b |
1307.17±6.181c |
1348.80±9.990c |
1344.80±3.361c |
|
28 |
1482.50±17.780a |
1303.90±14.632b |
1482.10±19.43a |
1328.60±12.710b |
1201.40±12.114c |
1377.60±22.96b |
|
|
35 |
1491.10±27.841a |
1289.00±15.462b |
1260.80±18.7b |
1485.00±12.227a |
1447.60±17.893a |
1214.00±19.28b |
|
|
42 |
1288.80±14.021a |
1126.50±19.803b |
1108±21.130b |
1644.30±20.360c |
1443.80±11.870d |
1332.95±10.25a |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; CK-MB: creatine kinase MB; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
All ECG leads (6 leads) of the broilers in various groups seen in Figure 1. The durations and amplitudes of all waves in lead II are presented in Table 5.
Figure 1. Samples of different electrocardiograms in experimental groups (CM, CP, M500, M2500, P500 and P2500) with six simultaneously recorded leads.
T duration in CP was significantly higher than in CM, M500, and M2500 (P<0.05). The duration of the T wave significantly decreased in P500 and P2500 compared to CP (P<0.05). The amplitude of the S wave significantly increased in CP compared to M500 and CM. The S-wave amplitude was significantly lower in P2500 than in CP on days 28 and 42. The amplitude of the T wave was higher in CP than in CM on day 42.
Receiving the supplemented pellet diet with 500 ppm of silymarin in P500 group decreased T-wave amplitude compared to CP group on days 35 and 42. The R-R interval in CP significantly decreased compared with CM. Birds in the P2500 group had a higher R-R interval compared with the CP group on day 42. The ST segment increased in CP compared to CM, M500, and M2500. In P500 and P2500, the ST segment was lower compared with CP. HR increased significantly in CP compared to CM, and P2500 had lower HR compared with CP on day 42 (Table 5).
Table 5 Electrocardiographic parameters (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
P-duration (S) |
21 |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.011a |
0.02±0.010a |
|
28 |
0.02±0.011a |
0.02±0.010a |
0.04±0.011b |
0.02±0.010a |
0.02±0.011a |
0.02±0.010a |
|
|
35 |
0.02±0.010a |
0.04±0.011b |
0.02±0.010a |
0.03±0.011c |
0.02±0.010a |
0.02±0.011a |
|
|
42 |
0.02±0.012a |
0.02±0.010a |
0.02±0.011a |
0.02±0.012a |
0.02±0.010a |
0.02±0.011a |
|
|
S-duration (S) |
21 |
0.03±0.011a |
0.02±0.010b |
0.03±0.010a |
0.02±0.010b |
0.02±0.011b |
0.02±0.010b |
|
28 |
0.03±0.012a |
0.03±0.010a |
0.03±0.010a |
0.02±0.010b |
0.02±0.010b |
0.03±0.010a |
|
|
35 |
0.03±0.010a |
0.04±0.012b |
0.04±0.010b |
0.03±0.010a |
0.04±0.011b |
0.04±0.010b |
|
|
42 |
0.04±0.010a |
0.04±0.011a |
0.04±0.010a |
0.04±0.010a |
0.04±0.010a |
0.04±0.010a |
|
|
T-duration (S) |
21 |
0.04±0.010b |
0.03±0.011a |
0.03±0.011a |
0.05±0.011c |
0.04±0.010b |
0.04±0.011b |
|
28 |
0.05±0.010c |
0.04±0.011a |
0.04±0.010a |
0.07±0.010d |
0.06±0.011b |
0.05±0.011c |
|
|
35 |
0.06±0.010d |
0.05±0.010a |
0.05±0.010a |
0.07±0.010b |
0.07±0.010b |
0.05±0.010c |
|
|
42 |
0.06±0.010c |
0.05±0.010a |
0.05±0.010a |
0.08±0.010d |
0.07±0.010b |
0.05±0.010a |
|
|
P-amplitude (mV) |
21 |
0.05±0.011a |
0.05±0.010a |
0.04±0.011b |
0.04±0.010b |
0.03±0.010c |
0.05±0.011a |
|
28 |
0.05±0.010a |
0.05±0.010a |
0.05±0.011a |
0.05±0.011a |
0.05±0.010a |
0.05±0.011a |
|
|
35 |
0.05±0.010a |
0.05±0.011a |
0.05±0.011a |
0.05±0.010a |
0.05±0.010a |
0.05±0.010a |
|
|
42 |
0.05±0.010a |
0.05±0.011a |
0.05±0.011a |
0.05±0.010a |
0.05±0.010a |
0.05±0.010a |
|
|
S-amplitude (mV) |
21 |
0.07±0.030c |
0.05±0.011a |
0.08±0.020b |
0.08±0.020b |
0.07±0.021c |
0.08±0.021b |
|
28 |
0.05±0.010b |
0.07±0.020a |
0.05±0.010b |
0.12±0.020d |
0.10±0.010c |
0.05±0.010b |
|
|
35 |
0.08±0.040a |
0.08±0.040a |
0.05±0.010b |
0.07±0.020d |
0.10±0.010c |
0.10±0.011c |
|
|
42 |
0.08±0.020c |
0.07±0.020a |
0.10±0.010b |
0.10±0.010b |
0.10±0.010b |
0.07±0.021a |
|
|
T-amplitude (mV) |
21 |
0.08±0.021c |
0.06±0.030a |
0.06±0.011a |
0.06±0.031a |
0.10±0.010b |
0.10±0.041b |
|
28 |
0.09±0.010d |
0.08±0.021a |
0.06±0.021b |
0.05±0.011c |
0.06±0.010b |
0.06±0.011b |
|
|
35 |
0.08±0.021c |
0.13±0.021a |
0.05±0.011b |
0.08±0.021c |
0.07±0.020d |
0.09±0.040e |
|
|
42 |
0.07±0.020d |
0.07±0.020a |
0.07±0.021a |
0.08±0.021b |
0.05±0.000c |
0.07±0.021d |
|
|
QT-Interval (S) |
21 |
0.10±0.010a |
0.10±0.011a |
0.10±0.011a |
0.10±0.010a |
0.10±0.011a |
0.10±0.010a |
|
28 |
0.10±0.010a |
0.09±0.011a |
0.10±0.011a |
0.11±0.010a |
0.11±0.011a |
0.10±0.001a |
|
|
35 |
0.11±0.010a |
0.11±0.011a |
0.10±0.010a |
0.12±0.010a |
0.11±0.010a |
0.11±0.001a |
|
|
42 |
0.11±0.010a |
0.10±0.011a |
0.11±0.011a |
0.12±0.001a |
0.12±0.010a |
0.11±0.001a |
|
|
RR-Interval (S) |
21 |
0.12±0.011b |
0.13±0.011a |
0.13±0.010a |
0.13±0.012a |
0.13±0.011a |
0.12±0.011b |
|
28 |
0.13±0.011b |
0.14±0.010a |
0.14±0.011a |
0.14±0.012a |
0.14±0.012a |
0.14±0.012a |
|
|
35 |
0.14±0.011c |
0.15±0.010a |
0.15±0.012a |
0.14±0.012c |
0.16±0.012b |
0.14±0.013c |
|
|
42 |
0.15±0.011b |
0.14±0.010a |
0.14±0.011a |
0.14±0.013a |
0.14±0.011a |
0.15±0.011b |
|
|
ST-segment (S) |
21 |
0.02±0.012a |
0.02±0.011a |
0.02±0.010a |
0.03±0.010b |
0.03±0.010b |
0.03±0.011b |
|
28 |
0.03±0.012a |
0.03±0.011a |
0.02±0.010b |
0.04±0.011c |
0.04±0.010c |
0.03±0.010a |
|
|
35 |
0.02±0.011d |
0.03±0.011a |
0.03±0.010a |
0.07±0.011e |
0.06±0.021b |
0.05±0.011c |
|
|
42 |
0.04±0.011d |
0.03±0.010a |
0.02±0.010b |
0.06±0.010e |
0.05±0.010c |
0.04±0.011d |
|
|
PR-interval (S) |
21 |
0.03±0.011c |
0.02±0.011a |
0.04±0.011b |
0.02±0.011a |
0.02±0.010a |
0.02±0.010a |
|
28 |
0.03±0.012a |
0.03±0.011a |
0.03±0.011a |
0.03±0.010a |
0.02±0.010b |
0.02±0.010b |
|
|
35 |
0.02±0.012c |
0.03±0.011a |
0.04±0.010b |
0.03±0.010a |
0.04±0.010b |
0.02±0.010c |
|
|
42 |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.011a |
0.02±0.011a |
|
|
Heart rate (Beats/min) |
21 |
500±31.650b |
468.75±32.562a,b |
447.76±22.986a |
458.01±20.342a |
468.75±20.451a,b |
500.00±19.781b |
|
28 |
458.01±20.450a |
428.57±19.872 b |
428.57± 16.870b |
428.57±18.092b |
428.57±18.761b |
428.57±20.181b |
|
|
35 |
413.76±18.721b |
397.35±18.234a,c |
405.40±13.541b,c |
413.76±17.102b |
375.00±12.091a |
428.57±19.319b |
|
|
42 |
389.61±13.661c |
428.57±16.626a,b |
428.57±10.982a,b |
413.76±19.110a |
437.95±11.541b |
387.76±13.090b |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
Silymarin has potent antioxidant activity, and the inhibition of oxidative damage by silymarin could be responsible for the protective effect on various organ disorders, such as the liver and heart (Razavi & Karimi, 2016). Main damages in broilers with RVF and ascites syndrome were seen in the liver and heart (Ahmadipour et al., 2019). The present study was designed to assess the positive effect of silymarin extract on liver and heart health in broilers fed on mash and pellet diets.
In this study, circulating total protein was significantly lower in the CP group than in the CM group, and silymarin at two doses (500 and 2500 ppm) in P500 and P2500 increased the serum level of total protein compared to the un-supplemented pellet diet (CP) on days 28, 35, and 42. In the present study, CP had significantly lower circulating albumin compared to CM on day 42, and supplementing with silymarin increased the serum level of albumin in P500 and P2500, but it was not statistically significant (P>0.05).
Albumin is the most abundant plasma protein, which is synthesized by the liver and has an important role in maintaining the plasma oncotic pressure within the vascular compartments, preventing the leaking of fluids into the extravascular spaces (Soeters et al., 2018). Hypoproteinemia and hypoalbuminemia were seen in various conditions, such as severe hepatic impairment, heart failure, nephrotic syndrome, malnutrition, and inflammation (Walayat et al., 2017; Soeters et al., 2018). Several previous researchers have stated that hepatic disorders in poultry led to hypoproteinemia and hypoalbuminemia, which these conditions result in fluid leakage into the extravascular spaces and the incidence of ascites (Lutensko et al., 2008; Milsavljevic, 2014).
Further, in agreement with our finding, Wang et al. (2018) showed that silymarin increased serum total protein in animal models with liver disorders. Also, Lutensko et al. (2008) and Baradaran et al. (2019) reported that silymarin increased the serum level of total protein in broiler chicks with liver disorders. It is established that silymarin stimulates protein synthesis through activating the RNA synthesis of ribosomes and improves body protein metabolism (Vargas-Mendoza et al., 2014). Based on these reports, a decrease in serum levels of protein and albumin in the CP group of the present study may be due to liver dysfunction, and silymarin improved protein metabolism due to its hepatoprotective action.
In the present study, the serum concentration of uric acid was significantly higher in CP than in CM on days 21, 28, and 42. Uric acid is one of the serum parameters, which increases in hepatocellular damages (Zaefarian et al., 2019). In the current research, circulating uric acid significantly decreased in P500 and P2500 groups compared with the CP group that showed the protective effects of silymarin on liver health in broilers fed on a pellet diet.
In the current experiment, the activities of AST and ALT enzymes in serum significantly increased in CP compared to CM on day 42, and supplementary silymarin at dose 2500 ppm significantly decreased the serum activities of these enzymes in the P2500 group. The serum activity levels of AST and ALT are an indicator of liver damages in birds (Senanayake et al., 2015). Several studies have shown that ALT and AST increase in ascetic birds due to cardiac and hepatic damages (Arab et al., 2006; Fathi et al., 2015).
In the current study, supplementation with silymarin in birds fed on a pellet diet decreased the serum activities of ALT and AST enzymes, but this decrease was more significant in P2500. In agreement with these results, several studies have shown that silymarin has a hepatoprotective effect and decreases the serum AST and ALT activities in broilers with damaged liver (Fani Makki et al., 2014, Wang et al., 2018).
The protective effect of silymarin on the liver has been more related to antioxidant effects, which eliminates free radicals. In addition, this substance leads to increased RNA polymerase activity in the nucleus of hepatocytes and elevated ribosomal protein synthesis, which improves hepatic cell regeneration and can inhibit lipid peroxidation, reduce glutathione oxidation, and enhance liver detoxification (Moayedi Esfahani et al., 2015). Therefore, silymarin as a free radical chelating agent may be effective in hepatic disorders of RVF and ascites syndrome.
In this experiment, silymarin decreased serum concentrations of triglyceride, total cholesterol, LDL, and VLDL in M500, M2500, P500, and P2500 groups compared to control groups (CM and CP). Gobalakrishnan et al. (2016) and Wang et al. (2018) established that silymarin has a hypolipidemic effect.
Silymarin enhances hepatic bile acid synthesis and stimulates the excretion of bile acids to the intestine. Silymarin increases endogenous cholesterol conversion to bile acids and stimulates the production of hepatic LDL receptors, leading to increased clearance of plasma LDL, HDL, and indirectly that of VLDL (Gobalakrishnan et al., 2016). Also, silymarin was able to inhibit the biosynthesis of cholesterol in the liver and reduce LDL cholesterol oxidation (Wang et al., 2018).
Various studies have been performed on the hepatoprotective action of silymarin in birds, but there are few studies on the protective effect of silymarin on heart health. However, the cardioprotective activity of silymarin has been established on laboratory animals in several experiments (Rašković et al., 2011; Razavi & Karimi, 2016; Vilahur et al., 2018).
In the present research, serum CK activity increased significantly in the CP group compared to the CM group, and the activity of CK-MB decreased significantly in birds treated with 2500 ppm silymarin on day 42. The high serum activity levels of AST and CK are an indication of cardiac injuries in birds (Bodor, 2016). Several studies have shown that CK increased in ascetic birds due to cardiac damages (Arab et al., 2006; Fathi et al., 2015).
In the present study, the simultaneous increase in AST and CK in the CP group may be due to heart injury, which a high level of CK attenuated in the supplemented pellet group with silymarin. In the current research, circulating levels of calcium, phosphorus, and sodium were significantly lower in the CP group than in other groups on 28, 35, and 42 days. Also, birds in the CP group had lower circulating potassium and magnesium compared to the CM group, but it was not statistically significant on day 42.
Heart failure usually leads to acid-base and electrolyte disturbance due to the activation of several neurohumoral mechanisms. The most common electrolyte abnormalities in heart failure are hyponatremia, hypokalemia, and hypomagnesemia (Hanton et al., 2007). Silymarin at doses 500 and 2500 ppm increases the concentrations of calcium and phosphorus in P500 and P2500 groups compared to CP group. In the present experiment, silymarin at different doses had no improvement effect on electrolyte abnormalities in birds fed on a pellet diet. In agreement with obtained results, Lutensko et al. (2008) reported that silymarin increased serum levels of calcium and phosphorus.
In this study, T amplitude and T duration in the CM group were significantly lower compared to the CP group on day 42, and silymarin at dose 2500 ppm decreased significantly the duration and amplitude of the T wave in P2500 compared to CP and P500 groups. In the present study, supplementing a pelleted diet by silymarin at two different doses P500 and P2500 decreased traits of the T wave, but this effect was greater and significant in dose 2500 ppm (P2500 group). Several studies have shown that RVF in broilers changes the ECG waves’ pattern, especially in R, S, and T waves (Hassanpour et al., 2009; Yousefi et al., 2013; Sharifi et al., 2015). Hassanpour et al. (2005) stated that the duration of the T wave was enhanced in the broilers susceptible to pulmonary hypertension syndrome PHS and ascites.
In this study, the amplitude of the S wave significantly increased in CP compared with M500 and CM. The S-wave amplitude was significantly lower in P2500 than in CP on days 28 and 42. Previous studies have reported that an increase in the S amplitude reflected the hypertrophy of RVF and ascites (Hassanpour et al., 2009; Yousefi et al., 2013; Sharifi et al., 2015).
In this research, S duration on days 21 and 28 was shorter in the CP group than in CM, 500 and M2500 groups, and P22500had a significantly longer duration of the S wave 500, and M2500 on different days of the trial. In P500 and P2500, the ST segment was lower than in CP on all days.
In contrast to our research, Hassanpour et al. (2009) reported that the ST interval had no changes in broilers with RVF. In this research, the R-R interval in CP significantly decreased compared to CM. Also, P2 had a higher R-R interval than CP on day 42. The obtained results showed that HR significantly increased in CP compared to CM, and P2 had a lower HR than CP on day 42. Contrary to our results, Olkowski (2007) stated that HR decreased in broiler chickens susceptible to pulmonary hypertension and ascites syndrome. They showed that progressive bradycardia was seen in these birds due to circulatory insufficiency.
Our finding showed that feeding with a pellet diet changed some ECG parameters and serum activity of CK-MB enzyme in broilers, which can indicate heart disorders in birds fed on a pellet diet, and silymarin extract could modulate these changes. The main property of silymarin is the antioxidant activity of its flavonolignans and other polyphenolic constituents, which is attributable to free radical scavenging.
Silymarin leads to replenish endogenous antioxidant enzymes, suppress neutrophil infiltration, and reduce serum malondialdehyde as an end product of myocardial lipid peroxides (Moayedi Esfahani et al., 2015). Previous studies have stated that silymarin has cardioprotective effects in animal models due to lipid peroxidation inhibition and an increase in antioxidant enzymes production in heart tissue (Gabrielová et al., 2019).
Based on our results, silymarin had a protective effect on heart function and reversed ECG indices and serum biochemical changes in serum in fast-growing broilers. Thus, silymarin can be a clinical strategy to decrease the incidence of heart failure and ascites-induced mortality and has a financial benefit in the poultry industry.
In conclusion, it seems that using a pelleted diet in fast-growing broiler chickens could increase the susceptibility of broilers to liver and heart disorders and change some blood biochemical parameters and ECG values. Also, the present findings confirmed that silymarin had a dose-dependent protective effect on heart and liver health in broilers and attenuated induced negative changes using a pellet diet. Thus, silymarin can be used as a cardioprotective and hepatoprotective compound in broilers susceptible to ascites syndrome for decreasing ascites-induced mortality.
The authors are grateful to Shiraz University Shiraz, Iran, for financial supporting this study.
The authors reported no conflict of interest.
The genetic selection has led to a fast growth rate in modern strains of broiler chickens (Ahmadipour et al., 2015). In fast-growing broilers, the metabolic rate and the requirement of oxygen are increased, and the heart and lungs are not capable of providing enough oxygen, which causes metabolic disorders (Wideman et al., 2013). Right ventricular failure (RVF) and ascites syndrome are the most prominent metabolic disorders, commonly seen in broiler chickens with a high-growth rate (Ahmadipour et al., 2019).
Diet form (pellet, mash, and crumble) is one of the most important effective factors on the incidence of RVF and ascites in broilers (Sahraei, 2014). Broilers fed on the pellet diet present higher weight gain and better feed conversion ratio compared with those fed the mash diet (Amerah et al., 2008). Several studies have stated that susceptibility to RVF and ascites was higher in birds fed pelleted diets than in birds received mash diets (Jafarnejad et al., 2010; Sahraei, 2014). The heart and liver are two major damaged organs in RVF and ascites syndrome (Jafarnejad et al., 2010). Improving the health of the heart and liver using herbal extracts is a benefit and can be a clinical strategy to control RVF lesions and decrease RVF-induced mortality. Milk thistle is one of the more common medicinal herbs with protective effects on the liver and heart.
Milk thistle (Silybum marianum L., Asteraceae) is one of the most common herbs for the treatment of liver diseases (Schrieber et al., 2008). The seed and fruit of milk thistle contain silymarin that shows a variety of pharmacological activities, as well as antioxidant, anti-inflammatory, antibacterial, and antiviral properties in many experimental and clinical studies (Koçarslan et al., 2016).
Some studies have evaluated the positive effects of silymarin extract on growth performance and hepatic disorders in broilers (Schiavone et al., 2007; Kralik et al., 2015; Hosseinian et al., 2020). Further, various researches have established that silymarin had cardioprotective activity due to its antioxidant content in laboratory animals (Rao & Viswanath, 2007; Al-Rasheed et al., 2014; Koçarslan et al., 2016). However, there is a lack of knowledge about the positive effects of silymarin on broiler’s heart health. Evaluating heart electrical activity by electrocardiogram (ECG) is a simple method to assess heart health in birds (Yogeshpriya et al., 2018).
In veterinary medicine, there are several diagnostic tools for the evaluation of cardiac abnormalities, but ECG is one of the most useful and non-invasive techniques, which gives significant information about heart rate (HR), cardiac arrhythmias, and electrical conductance abnormalities (Cushing et al., 2013; Yogeshpriya et al., 2018).
In avian medicine, ECG is a useful tool utilized to measure HR and detect cardiac arrhythmias and cardiac chamber enlargement (Sharifi et al., 2015; Hosseinian et al., 2019). Several studies have indicated that RVF and ascites syndrome induce a change in the morphology of ECG waves in broilers, and they used ECG to detect and diagnosis the ascites syndrome (Hassanpour et al., 2009; Yousefi et al., 2013).
In this study, broilers were fed with two different forms of diet (mash and pellet), and heart and liver health was evaluated by assessing some serum biochemical and ECG induces. Also, the effects of two different doses of dietary silymarin (500 and 2500 ppm) were assessed on some serum parameters and ECG. In this research, this hypothesis was assessed that pellet diet can be led to heart and liver dysfunction in broilers, and silymarin could attenuate the cardiac and hepatic damages.
The results of the present study may clarify the protective effects of silymarin on the liver and heart in fast-growing broilers, and silymarin can be used as a cardioprotective and hepatoprotective compound during the rearing period of susceptible broilers to ascites syndrome.
This experiment was performed at the Veterinary College of Shiraz University. A total of 120 1-day-old Arbor Acres broiler chicks were used for the experiment and reared for 42 days. During study, broilers were raised in environmentally controlled rooms under standard environmental conditions suggested by commercial recommendations of the chick producer company (Aviagen, 2018).
On day 14 of the experiment, birds were weighed and randomly assigned into 6 equal groups (n=20) with five replicates. The treatment groups consisted of supplementation with silymarin extract in the mash and pelleted diets as follows: the control mash group (basal mash diet [CM], fed on a mash diet without silymarin), control pellet (basal pellet diet [CP], fed on a pellet diet without silymarin), two doses of silymarin in mash feed (silymarin at 500 ppm of mash [M500] and silymarin at 2500 ppm of mash [M2500]), and two doses of silymarin in pellet feed (silymarin at 500 ppm of pellet [P500] and silymarin at 2500 ppm of pellet [P2500]).
In the present study, the basal diet was corn-soybean meal-based and formulated to meet or exceed the minimum National Research Council (NRC; 1994) Standards. The starter (0-10 days), grower (10-25 days), and finisher (25-42 days) feeds were used during the experiment. All birds were fed on a pellet diet during the first and second weeks of the experiment, and then two forms of diet (mash and pellet) with a similar composition of ingredients (see Table 1) were used from the third to sixth weeks of the experiment.
Table 1. Ingredients and composition of mixture feed (gkg-1)
|
Component |
Diets |
||
|
|
Starter |
Grower |
Finisher |
|
Ingredients, % |
|
|
|
|
Yellow Corn |
53.4 |
58.0 |
61.3 |
|
Soybean meal (44%) |
39.0 |
35.0 |
31.0 |
|
Dicalcium phosphate |
1.7 |
1.5 |
1.3 |
|
CaCO3 (38%) |
1.7 |
1.4 |
1.3 |
|
Sunflower oil |
3.0 |
3.0 |
4.0 |
|
Sodium chloride |
0.3 |
0.3 |
0.3 |
|
Methionine |
0.2 |
0.15 |
0.15 |
|
Lysine |
0.2 |
0.15 |
0.15 |
|
Premix* |
0.5 |
0.5 |
0.5 |
|
Total |
100 |
100 |
100 |
|
Nutrient levels |
|
|
|
|
ME, kcal/kg |
2900 |
3000 |
3100 |
|
Crude protein, % |
22 |
20.5 |
19 |
|
Calcium, % |
1.05 |
0.9 |
0.8 |
|
Available Phosphorus, % |
0.5 |
0.45 |
0.4 |
|
Sodium, % |
0.19 |
0.19 |
0.19 |
|
Lysine, % |
1.31 |
1.25 |
1.14 |
|
Methionine, % |
0.54 |
0.48 |
0.44 |
|
Threonine, % |
0.85 |
0.81 |
0.75 |
|
Tryptophan, % |
0.25 |
0.22 |
0.2 |
* Vitamin and mineral content per kilogram of premix: vitamin A: 3,600,000 IU; vitamin D3: 800,000 IU; vitamin E: 7,200 IU; vitamin K3: 0.8 g; vitamin B1: 0.71 g; vitamin B2: 2.64 g; vitamin B3: 3.92 g, vitamin B5: 11.88 g; vitamin B6: 1.176 g; vitamin B12: 6 mg; folic acid: 0.4 g; biotin: 40 mg; choline chloride: 100 g; selenium: 80 mg; cobalt: 100 mg; iodine: 396 mg; copper:4 g; zinc: 33.88 g; iron: 20 g; manganese: 39.68 g.
The used diets in this study was prepared in the livestock affairs of the Faculty of Veterinary Medicine, Shiraz University. Firstly, the feed was supplied based on NRC Standards for broilers. Then, a part of the feed was finely grounded, mixed, and used as a mash form in mash treatments during the entire the experiment, and the other part of the feed was pelleted in a steam pellet mill. After pelleting, the pelleted feed was dried and cooled to an average temperature of 37°C and used as a pellet form in pellet treatments during the entire the experiment. Feed and freshwater were supplied ad libitum during the entire experiment.
Silymarin powder was purchased from BarijEsans Company (Iran), containing 43.87% of silibinin and 96.76% dry matter and was added to mash and pellet diets from days 14 to 42 of the experiment. All diets contained neither anticoccidials nor any other medications.
The temperature was maintained at 32°C during the first week and reduced gradually until a constant temperature of 24°C was achieved. A 24-hour lighting schedule was used during the first week and then reduced gradually until 20 h light: 4 h darkness (20L:4D).
Ten apparently healthy birds of each group were selected, and blood sampling (5 mL/birds) was done from the brachial vein on days 21, 28, 35, and 42. Blood samples were kept at room temperature for 30 minutes, and then the clotted blood samples were centrifuged at 3000g for 10 minutes to separate sera. The clear sera were collected and stored at -20℃ until the biochemical analysis.
The hepatic parameters, including total protein, albumin, globulin, uric acid, triglyceride, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL), were measured by an automated analyzer (Alpha Classic, Sanjesh Company, Iran) using commercial clinical investigation kits (Pars Azmoon, Tehran, Iran). Moreover, the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were evaluated using a commercial kit (Pars Azmoon, Tehran, Iran).
Biochemical Assays
Calcium, magnesium, phosphorous, chloride, and creatine kinase MB (CK-MB) were measured using commercial kits (Pars Azmoon, Iran) and biochemical auto analyzer (Alpha Classic, Sanjesh Company, Iran). Sodium and potassium were determined using a flame photometer (Clinical flame photometer, Fater Company, Iran).
From each group, 7 apparently healthy chicks were randomly selected on 21, 28, 35, and 42 days, and ECGs were recorded by a single channel ECG machine (Kenz-line EKG 110, Suzuken Company, Japan). The ECG measurements were obtained from unanesthetized and restrained birds in a standing position. Before ECG recording, the chickens rested for about 5 minutes to calm down. ECG gel was applied to the skin, and then alligator clip electrodes were positioned at the base of the right and left wings and gastrocnemius muscle of the right and left limbs (Yogeshpriya et al., 2018).
All ECGs were recorded using a calibration of 10 mm/mV and a paper speed of 50 mm/sec (Reddy et al., 2016). ECGs were recorded by different leads, including I, II, III, and aVR, aVL, and aVF for every chicken. In birds, lead II is commonly used to evaluate waves' morphology in ECG (Reddy et al., 2016). For this reason, the amplitude and duration of P, T, R, and S waves, as well as the duration of QT, R-R, PR, and ST intervals, in each bird of the present experiment were measured from lead II as a standard lead (Reddy et al., 2016). Finally, HR was measured in each bird.
The statistical analysis was performed using SPSS 22 (SPSS Inc., Chicago, Ill., USA). In the present study, the data from various serum parameters and ECG indices were analyzed by 1-way analysis of variance (ANOVA). The data were presented as mean ± SE. The significance level was set as P-value<0.05.
The results of serum hepatic parameters are presented in Tables 2 and 3.
As seen in Table 2, total protein and albumin significantly decreased in CP compared with CM at some times of the experiment (P<0.05). P500 and P2500 significantly had a higher total protein compared with CP on days 28, 35, and 42. Circulating uric acid increased significantly in CP and P500 compared with CM. In this experiment, P2500 had a lower level of uric acid compared to CP and P500. The serum activity of ALT and AST enzymes in the CP group was significantly higher than in CM, and the activity levels of ALT and AST decreased significantly in P2500 compared to CP on day 42 (P<0.05) (Table 2).
Table 2. Circulating hepatic parameters (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups (mean ±SE) |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
Total protein (gr/L) |
21 |
2.43±0.071a |
2.92±0.022a |
2.13±0.040a |
2.80±0.041a |
3.12±0.080b |
2.72±0.101a |
|
28 |
3.47±0.092a |
3.35±0.073a |
2.89±0.033b |
2.25±0.072b |
3.46±0.052a |
3.70±0.081a |
|
|
35 |
2.80±0.051a |
2.94±0.072a |
2.94±0.022a |
2.59±0.074a |
3.09±0.084b |
3.01±0.017b |
|
|
42 |
3.72±0.091a |
3.98±0.020a |
3.87±0.080a |
2.28±0.038b |
3.36±0.063a |
3.92±0.043a |
|
|
Albumin (gr/L) |
21 |
1.37±0.031a |
1.34±0.040a |
1.21±0.062a |
1.42±0.035a |
1.52±0.023a |
1.42±0.044a |
|
28 |
1.40±0.064a |
1.46±0.016a |
1.39±0.074a |
1.57±0.051a |
1.19±0.065a |
1.42±0.065a |
|
|
35 |
1.37±0.087a |
1.38±0.055a |
1.39±0.020a |
1.22±0.050a |
1.28±0.066a |
1.32±0.022a |
|
|
42 |
2.43±0.034a |
2.47±0.054a |
2.44±0.020a |
1.26±0.120b |
1.36±0.074b |
1.92±0.060b |
|
|
Globulin (gr/L) |
21 |
1.08±0.170a |
1.54±0.068a |
0.97±0.114a |
1.39±0.081a |
1.68±0.123a |
1.37±0.205a |
|
28 |
1.42±0.080a |
1.74±0.125a |
1.48±0.065a |
1.68±0.112a |
1.05±0.115a |
1.60±0.144a |
|
|
35 |
1.53±0.111a |
1.61±0.164a |
1.25±0.176a |
1.69±0.142a |
1.63±0.121a |
1.68±0.135a |
|
|
42 |
1.27±0.201a |
1.58±0.097a |
1.36±0.078a |
1.40±0.163a |
1.73±0.130a |
1.39±0.084a |
|
|
Uric acid (mg/dL) |
21 |
2.83±0.225a |
3.58±0.189b |
2.85±0.197a |
3.23±0.305b |
4.60±0.404c |
3.98±0.513b |
|
28 |
3.22±0.297a |
3.95±0.348a |
3.85±0.135a |
4.51±0.434b |
2.71±0.175c |
3.80±0.305a |
|
|
35 |
3.94±0.271a |
3.92±0.313a |
4.09±0.491a |
3.72±0.300a |
4.55±0.424b |
3.71±0.294a |
|
|
42 |
3.90±0.519a |
4.21±0.190b |
3.28±0.294a |
4.26±0.455b |
4.08±0.313b |
3.20±0.315a |
|
|
Alanine aminotransferase (IU/L) |
21 |
126.84±9.110a |
122.60±9.158a |
99.45±3.780b |
125.39±8.937a |
121.44±4.844a |
128.55±4.385a |
|
28 |
127.88±7.910a |
129.29±7.856a |
110.88±6.125b |
132.79±3.809a |
129.98±4.496a |
125.01±6.147a |
|
|
35 |
128.04±9.747a |
124.55±6.144a |
97.58±3.751b |
132.88±6.568a |
119.57±4.728a |
109.29±6.446a |
|
|
42 |
97.88±5.887a |
112.45±6.783b |
109.11±5.360b |
128.19±4.018b |
130.01±3.950b |
95.48±5.010a |
|
|
Aspartate aminotransferase (IU/L) |
21 |
253.70±10.317a |
245.60±10.958a |
199.90±7.888b |
258.30±8.178a |
242.90±9.981a |
248.30±6.480a |
|
28 |
252.80±8.015a |
257.20±8.154a |
240.00±12.125b |
265.70±6.906a |
259.90±7.410a |
249.70±10.744a |
|
|
35 |
240.30±10.846a |
242.40±11.541a |
193.20±6.785b |
263.50±11.765a |
229.70±7.027a |
203.20±12.033b |
|
|
42 |
199.50±6.812a |
219.50±7.683b |
207.90±8.664a |
241.30±6.112b |
247.90±8.255b |
198.60±9.050a |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
As seen in Table 3, the serum levels of total triglyceride, cholesterol, and LDL in supplemented groups (i.e., M500, M2500, P500, and P2500) decreased significantly compared to CM and CP groups (P<0.05). The CP group had lower concentrations of VLDL and LDL compared to the CM group.
Table 3Circulating hepatic indices (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups (mean ±SE) |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
Triglyceride (mg/dL) |
21 |
85.70±1.820a |
87.70±3.285a |
62.80±1.655b |
83.30±3.850a |
77.50±1.22b |
63.00±2.35b |
|
28 |
85.70±3.180a |
71.50±1.980b |
79.00±3.996b |
72.50±1.544b |
55.80±1.96c |
66.90±1.49b |
|
|
35 |
76.40±1.431a |
64.70±1.321b |
66.20±2.027b |
84.90±1.242c |
65.40±1.65b |
63.40±3.91b |
|
|
42 |
83.80±1.262a |
73.50±1.825b |
62.40±3.221c |
89.10±2.19a |
72.50±1.65b |
60.40±3.91c |
|
|
Cholesterol (mg/dL) |
21 |
148.50±2.39a |
100.80±6.32b |
98.50±2.551b |
145.60±2.16a |
101.50±2.54b |
103.20±3.1b |
|
28 |
148.50±2.71a |
122.80±2.51b |
122.40±2.03b |
144.30±3.74a |
96.10±2.640c |
122.50±3.8b |
|
|
35 |
139.40±2.64a |
110.70±3.48b |
107.60±4.62b |
139.50±3.09a |
100.70±3.49b |
97.20±3.87b |
|
|
42 |
135.20±1.24a |
116.00±2.13b |
105.40±2.24c |
143.20±3.20a |
113.90±1.71b |
93.30±2.58c |
|
|
HDL (mg/dL) |
21 |
70.60±1.07a |
78.20±0.284a |
71.10±1.425a |
70.90±0.704a |
71.70±1.094a |
74.20±0.520a |
|
28 |
70.90±0.92a |
72.60±0.921a |
76.00±0.285a |
71.40±1.175a |
70.30±0.975a |
77.30±0.634a |
|
|
35 |
75.30±0.25a |
70.30±0.908a |
70.80±1.034a |
75.00±0.870a |
76.00±0.891a |
70.90±0.910a |
|
|
42 |
67.10±1.09a |
74.20±0.169a |
71.30±0.363a |
70.00±1.954a |
73.10±1.208a |
71.40±1.097a |
|
|
LDL (mg/dL) |
21 |
93.00±2.23a |
58.70±2.41b |
54.80±1.27b |
81.20±1.944c |
55.90±1.50b |
51.00±1.74b |
|
28 |
85.60±0.64a |
54.00±1.38b |
59.60±1.34b |
77.70±1.485a |
53.40±0.21b |
60.50±1.47b |
|
|
35 |
89.80±0.45a |
53.20±2.43b |
49.50±2.20b |
71.70±1.853c |
56.70±1.79b |
52.70±1.30b |
|
|
42 |
91.50±1.40a |
55.80±2.187c |
49.90±0.372c |
75.60±1.05b |
50.50±1.660c |
57.70±1.950c |
|
|
VLDL (mg/dL) |
21 |
17.06±0.43a |
18.13±0.588a |
12.54±0.638c |
15.52±0.95b |
15.68±0.25b |
12.65±0.594c |
|
28 |
17.19±0.67a |
14.29±0.18b |
16.58±0.780a |
14.99±0.36b |
11.05±0.966c |
13.28±0.49b |
|
|
35 |
15.24±0.48a |
12.98±0.52b |
13.24±0.45b |
16.90±0.818a |
13.80±0.95b |
12.65±0.73b |
|
|
42 |
16.80±0.36a |
14.72±0.37b |
12.40±0.676c |
17.80±0.890a |
14.54±0.190b |
12.31±0.760c |
|
|
Total billirubin (mg/dL) |
21 |
0.46±0.024c |
0.51±0.014b,c |
0.42±0.011a,c |
0.50±0.074b |
0.55±0.104b |
0.48±0.081a,c |
|
28 |
0.48±0.078b |
0.51±0.076a,b |
0.51±0.050a,b |
0.56±0.090a |
0.44±0.054c |
0.49±0.041b,c |
|
|
35 |
0.51±0.017a |
0.51±0.074a |
0.50±0.104a |
0.50±0.012a |
0.54±0.031a |
0.50±0.032a |
|
|
42 |
0.46±0.019a |
0.58±0.020b |
0.50±0.024a |
0.51±0.090a,b |
0.53±0.080a,b |
0.49±0.071a,b |
|
|
Direct billirubin (mg/dL) |
21 |
0.03±0.011a,b |
0.02±0.003b |
0.03±0.011a,b |
0.03±0.004a,b |
0.02±0.006a,b |
0.04±0.001a |
|
28 |
0.04±0.020a |
0.04±0.003a |
0.03±0.021a |
0.03±0.007a |
0.03±0.003a |
0.03±0.002a |
|
|
35 |
0.03±0.000a |
0.04±0.001a |
0.03±0.002a |
0.03±0.005a |
0.04±0.004a |
0.04±0.003a |
|
|
42 |
0.04±0.002a |
0.03±0.011a |
0.03±0.004a |
0.03±0.004a |
0.03±0.003a |
0.03±0.001a |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; HDL: high-density lipoprotein cholesterol; LDL: low-density lipoprotein cholesterol; VLDL: very low-density lipoprotein cholesterol; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
The results of serum cardiac parameters are presented in Table 4.
The circulating levels of calcium and phosphorus were significantly lower in the CP group than in other groups on 28, 35, and 42 days (P<0.05). P500 and P2500 had significantly higher concentrations of calcium and phosphorus compared with CP (P<0.05). CP had a higher activity of CK enzyme compared with CM on day 42, and P500 and P2500 had a lower serum activity of CK compared with CP (P<0.05) (Table 4).
Table 4Circulating electrolytes and cardiac parameters (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups (mean ±SE ) |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
Calcium ( mg/dL) |
21 |
5.71±0.180a |
5.09±0.354a |
3.99±0.354b |
5.28±0.091a |
6.66±1.771c |
5.7±0.721a |
|
28 |
7.28±0.020a |
7.39±0.092a |
6.94±0.101a |
5.45±0.011b |
6.88±0.082a |
7.09±0.062a |
|
|
35 |
6.31±0.116a |
6.03±0.093a |
6.57±0.230a |
4.28±0.081b |
6.36±0.482a |
6.32±0.240a |
|
|
42 |
7.79±0.064a |
7.79±0.151a |
7.05±0.192a |
5.14±0.060b |
7.22±0.033a |
7.84±0.120a |
|
|
Phosphorus (mg/dL) |
21 |
4.90±0.351a |
4.79±0.350a |
4.56±0.231a |
5.15±0.304a |
5.23±0.304a |
4.79±0.323a |
|
28 |
6.09±0.101a |
5.32±0.111a |
5.63±0.122a |
4.63±0.151b |
4.61±0.082b |
5.80±0.062a |
|
|
35 |
5.59±0.102a |
5.35±0.082a |
5.77±0.113a |
3.17±0.052b |
5.05±0.044a |
5.25±0.060a |
|
|
42 |
6.72±1.130a |
5.99±0.203a |
5.12±0.314b |
4.78±0.343b |
5.67±0.332a |
6.89±0.330a |
|
|
Sodium (mmol/L) |
21 |
125.53±3.880a |
122.33±5.540a |
129.76±5.34a |
119.98±8.981a |
111.28±3.990a |
113.98±6.184a |
|
28 |
130.45±4.431a |
129.87±7.011a |
138.65±9.89a |
112.23±6.941b |
119.63±5.844b |
115.23±6.44b |
|
|
35 |
132.98±5.211a |
132.13±8.902a |
128.51±7.09a |
119.98±7.862b |
129.47±7.012a |
142.78±6.961c |
|
|
42 |
140.89±10.09a |
132.44±5.985a |
130.87±9.89a |
123.67±9.321b |
119.17±8.330b |
143.97±7.920a |
|
|
Potassium (mg/dL) |
21 |
4.87±1.06a |
4.56±2.032a |
5.01±1.234a |
4.88±0.985a |
4.77±1.094a |
4.61±0.285a |
|
28 |
4.78±0.984a |
3.99±1.014a |
4.66±2.135a |
4.35±0.691a |
4.98±1.971a |
4.09±0.325a |
|
|
35 |
4.90±0.214a |
4.52±1.124a |
4.87±3.095a |
5.01±0.274a |
4.81±0.542a |
4.62±0.477a |
|
|
42 |
4.98±0.792a |
5.06±3.095a |
4.72±2.017a |
4.55±0.936a |
4.76±0.437a |
4.72±0.732a |
|
|
Magnesium (mg/dL) |
21 |
1.88±0.091a |
2.15±0.067a |
1.84±0.065a |
2.02±0.071a |
2.25±0.134a |
2.06±0.134a |
|
28 |
2.07±0.084a |
2.31±0.085a |
2.22±0.046a |
2.23±0.060a |
1.85±0.062a |
2.29±0.085a |
|
|
35 |
2.41±0.072a |
2.38±0.076a |
2.40±0.057a |
2.26±0.074a |
2.22±0.045a |
2.39±0.066a |
|
|
42 |
2.52±0.033a |
2.55±0.044a |
2.62±0.070a |
2.16±0.052a |
2.29±0.080a |
2.54±0.070a |
|
|
Chloride (mmol/L) |
21 |
105.30±0.225a |
103.90±0.455a |
107.50±0.445a |
105.70±0.512a |
107.50±0.664a |
106.80±0.53a |
|
28 |
106.70±0.650a |
108.70±0.346a |
105.80±0.323a |
104.60±0.380a |
110.30±0.415a |
106.30±0.47a |
|
|
35 |
107.80±0.642a |
109.40±0.854a |
103.90±0.327a |
104.50±0.514a |
108.50±0.865a |
108.30±0.44a |
|
|
42 |
104.80±0.337a |
106.20±0.473a |
105.10±0.610a |
103.80±0.515a |
107.40±0.534a |
108.30±0.49a |
|
|
CK-MB (IU/L) |
21 |
1481.80±12.890a |
1474.50±10.081a |
1235.22±13.06b |
1307.17±6.181c |
1348.80±9.990c |
1344.80±3.361c |
|
28 |
1482.50±17.780a |
1303.90±14.632b |
1482.10±19.43a |
1328.60±12.710b |
1201.40±12.114c |
1377.60±22.96b |
|
|
35 |
1491.10±27.841a |
1289.00±15.462b |
1260.80±18.7b |
1485.00±12.227a |
1447.60±17.893a |
1214.00±19.28b |
|
|
42 |
1288.80±14.021a |
1126.50±19.803b |
1108±21.130b |
1644.30±20.360c |
1443.80±11.870d |
1332.95±10.25a |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; CK-MB: creatine kinase MB; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
All ECG leads (6 leads) of the broilers in various groups seen in Figure 1. The durations and amplitudes of all waves in lead II are presented in Table 5.
Figure 1. Samples of different electrocardiograms in experimental groups (CM, CP, M500, M2500, P500 and P2500) with six simultaneously recorded leads.
T duration in CP was significantly higher than in CM, M500, and M2500 (P<0.05). The duration of the T wave significantly decreased in P500 and P2500 compared to CP (P<0.05). The amplitude of the S wave significantly increased in CP compared to M500 and CM. The S-wave amplitude was significantly lower in P2500 than in CP on days 28 and 42. The amplitude of the T wave was higher in CP than in CM on day 42.
Receiving the supplemented pellet diet with 500 ppm of silymarin in P500 group decreased T-wave amplitude compared to CP group on days 35 and 42. The R-R interval in CP significantly decreased compared with CM. Birds in the P2500 group had a higher R-R interval compared with the CP group on day 42. The ST segment increased in CP compared to CM, M500, and M2500. In P500 and P2500, the ST segment was lower compared with CP. HR increased significantly in CP compared to CM, and P2500 had lower HR compared with CP on day 42 (Table 5).
Table 5 Electrocardiographic parameters (mean±SE) of broiler chickens (n=20) following dietary supplementation of pellet and mash diets by silymarin extract.
|
Parameters |
Days |
Groups |
|||||
|
CM |
M500 |
M2500 |
CP |
P500 |
P2500 |
||
|
P-duration (S) |
21 |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.011a |
0.02±0.010a |
|
28 |
0.02±0.011a |
0.02±0.010a |
0.04±0.011b |
0.02±0.010a |
0.02±0.011a |
0.02±0.010a |
|
|
35 |
0.02±0.010a |
0.04±0.011b |
0.02±0.010a |
0.03±0.011c |
0.02±0.010a |
0.02±0.011a |
|
|
42 |
0.02±0.012a |
0.02±0.010a |
0.02±0.011a |
0.02±0.012a |
0.02±0.010a |
0.02±0.011a |
|
|
S-duration (S) |
21 |
0.03±0.011a |
0.02±0.010b |
0.03±0.010a |
0.02±0.010b |
0.02±0.011b |
0.02±0.010b |
|
28 |
0.03±0.012a |
0.03±0.010a |
0.03±0.010a |
0.02±0.010b |
0.02±0.010b |
0.03±0.010a |
|
|
35 |
0.03±0.010a |
0.04±0.012b |
0.04±0.010b |
0.03±0.010a |
0.04±0.011b |
0.04±0.010b |
|
|
42 |
0.04±0.010a |
0.04±0.011a |
0.04±0.010a |
0.04±0.010a |
0.04±0.010a |
0.04±0.010a |
|
|
T-duration (S) |
21 |
0.04±0.010b |
0.03±0.011a |
0.03±0.011a |
0.05±0.011c |
0.04±0.010b |
0.04±0.011b |
|
28 |
0.05±0.010c |
0.04±0.011a |
0.04±0.010a |
0.07±0.010d |
0.06±0.011b |
0.05±0.011c |
|
|
35 |
0.06±0.010d |
0.05±0.010a |
0.05±0.010a |
0.07±0.010b |
0.07±0.010b |
0.05±0.010c |
|
|
42 |
0.06±0.010c |
0.05±0.010a |
0.05±0.010a |
0.08±0.010d |
0.07±0.010b |
0.05±0.010a |
|
|
P-amplitude (mV) |
21 |
0.05±0.011a |
0.05±0.010a |
0.04±0.011b |
0.04±0.010b |
0.03±0.010c |
0.05±0.011a |
|
28 |
0.05±0.010a |
0.05±0.010a |
0.05±0.011a |
0.05±0.011a |
0.05±0.010a |
0.05±0.011a |
|
|
35 |
0.05±0.010a |
0.05±0.011a |
0.05±0.011a |
0.05±0.010a |
0.05±0.010a |
0.05±0.010a |
|
|
42 |
0.05±0.010a |
0.05±0.011a |
0.05±0.011a |
0.05±0.010a |
0.05±0.010a |
0.05±0.010a |
|
|
S-amplitude (mV) |
21 |
0.07±0.030c |
0.05±0.011a |
0.08±0.020b |
0.08±0.020b |
0.07±0.021c |
0.08±0.021b |
|
28 |
0.05±0.010b |
0.07±0.020a |
0.05±0.010b |
0.12±0.020d |
0.10±0.010c |
0.05±0.010b |
|
|
35 |
0.08±0.040a |
0.08±0.040a |
0.05±0.010b |
0.07±0.020d |
0.10±0.010c |
0.10±0.011c |
|
|
42 |
0.08±0.020c |
0.07±0.020a |
0.10±0.010b |
0.10±0.010b |
0.10±0.010b |
0.07±0.021a |
|
|
T-amplitude (mV) |
21 |
0.08±0.021c |
0.06±0.030a |
0.06±0.011a |
0.06±0.031a |
0.10±0.010b |
0.10±0.041b |
|
28 |
0.09±0.010d |
0.08±0.021a |
0.06±0.021b |
0.05±0.011c |
0.06±0.010b |
0.06±0.011b |
|
|
35 |
0.08±0.021c |
0.13±0.021a |
0.05±0.011b |
0.08±0.021c |
0.07±0.020d |
0.09±0.040e |
|
|
42 |
0.07±0.020d |
0.07±0.020a |
0.07±0.021a |
0.08±0.021b |
0.05±0.000c |
0.07±0.021d |
|
|
QT-Interval (S) |
21 |
0.10±0.010a |
0.10±0.011a |
0.10±0.011a |
0.10±0.010a |
0.10±0.011a |
0.10±0.010a |
|
28 |
0.10±0.010a |
0.09±0.011a |
0.10±0.011a |
0.11±0.010a |
0.11±0.011a |
0.10±0.001a |
|
|
35 |
0.11±0.010a |
0.11±0.011a |
0.10±0.010a |
0.12±0.010a |
0.11±0.010a |
0.11±0.001a |
|
|
42 |
0.11±0.010a |
0.10±0.011a |
0.11±0.011a |
0.12±0.001a |
0.12±0.010a |
0.11±0.001a |
|
|
RR-Interval (S) |
21 |
0.12±0.011b |
0.13±0.011a |
0.13±0.010a |
0.13±0.012a |
0.13±0.011a |
0.12±0.011b |
|
28 |
0.13±0.011b |
0.14±0.010a |
0.14±0.011a |
0.14±0.012a |
0.14±0.012a |
0.14±0.012a |
|
|
35 |
0.14±0.011c |
0.15±0.010a |
0.15±0.012a |
0.14±0.012c |
0.16±0.012b |
0.14±0.013c |
|
|
42 |
0.15±0.011b |
0.14±0.010a |
0.14±0.011a |
0.14±0.013a |
0.14±0.011a |
0.15±0.011b |
|
|
ST-segment (S) |
21 |
0.02±0.012a |
0.02±0.011a |
0.02±0.010a |
0.03±0.010b |
0.03±0.010b |
0.03±0.011b |
|
28 |
0.03±0.012a |
0.03±0.011a |
0.02±0.010b |
0.04±0.011c |
0.04±0.010c |
0.03±0.010a |
|
|
35 |
0.02±0.011d |
0.03±0.011a |
0.03±0.010a |
0.07±0.011e |
0.06±0.021b |
0.05±0.011c |
|
|
42 |
0.04±0.011d |
0.03±0.010a |
0.02±0.010b |
0.06±0.010e |
0.05±0.010c |
0.04±0.011d |
|
|
PR-interval (S) |
21 |
0.03±0.011c |
0.02±0.011a |
0.04±0.011b |
0.02±0.011a |
0.02±0.010a |
0.02±0.010a |
|
28 |
0.03±0.012a |
0.03±0.011a |
0.03±0.011a |
0.03±0.010a |
0.02±0.010b |
0.02±0.010b |
|
|
35 |
0.02±0.012c |
0.03±0.011a |
0.04±0.010b |
0.03±0.010a |
0.04±0.010b |
0.02±0.010c |
|
|
42 |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.010a |
0.02±0.011a |
0.02±0.011a |
|
|
Heart rate (Beats/min) |
21 |
500±31.650b |
468.75±32.562a,b |
447.76±22.986a |
458.01±20.342a |
468.75±20.451a,b |
500.00±19.781b |
|
28 |
458.01±20.450a |
428.57±19.872 b |
428.57± 16.870b |
428.57±18.092b |
428.57±18.761b |
428.57±20.181b |
|
|
35 |
413.76±18.721b |
397.35±18.234a,c |
405.40±13.541b,c |
413.76±17.102b |
375.00±12.091a |
428.57±19.319b |
|
|
42 |
389.61±13.661c |
428.57±16.626a,b |
428.57±10.982a,b |
413.76±19.110a |
437.95±11.541b |
387.76±13.090b |
|
CM: control mash diet (0 ppm silymarin); CP: control pellet diet (0 ppm silymarin); M500: mash diet + 500 ppm silymarin; M2500: mash diet + 2500 ppm silymarin; P500: pellet diet + 500 ppm silymarin; P2500: pellet diet + 2500 ppm silymarin; a,b,c Different letters in the superscripts of the same row indicate significant differences (P <0.05).
Silymarin has potent antioxidant activity, and the inhibition of oxidative damage by silymarin could be responsible for the protective effect on various organ disorders, such as the liver and heart (Razavi & Karimi, 2016). Main damages in broilers with RVF and ascites syndrome were seen in the liver and heart (Ahmadipour et al., 2019). The present study was designed to assess the positive effect of silymarin extract on liver and heart health in broilers fed on mash and pellet diets.
In this study, circulating total protein was significantly lower in the CP group than in the CM group, and silymarin at two doses (500 and 2500 ppm) in P500 and P2500 increased the serum level of total protein compared to the un-supplemented pellet diet (CP) on days 28, 35, and 42. In the present study, CP had significantly lower circulating albumin compared to CM on day 42, and supplementing with silymarin increased the serum level of albumin in P500 and P2500, but it was not statistically significant (P>0.05).
Albumin is the most abundant plasma protein, which is synthesized by the liver and has an important role in maintaining the plasma oncotic pressure within the vascular compartments, preventing the leaking of fluids into the extravascular spaces (Soeters et al., 2018). Hypoproteinemia and hypoalbuminemia were seen in various conditions, such as severe hepatic impairment, heart failure, nephrotic syndrome, malnutrition, and inflammation (Walayat et al., 2017; Soeters et al., 2018). Several previous researchers have stated that hepatic disorders in poultry led to hypoproteinemia and hypoalbuminemia, which these conditions result in fluid leakage into the extravascular spaces and the incidence of ascites (Lutensko et al., 2008; Milsavljevic, 2014).
Further, in agreement with our finding, Wang et al. (2018) showed that silymarin increased serum total protein in animal models with liver disorders. Also, Lutensko et al. (2008) and Baradaran et al. (2019) reported that silymarin increased the serum level of total protein in broiler chicks with liver disorders. It is established that silymarin stimulates protein synthesis through activating the RNA synthesis of ribosomes and improves body protein metabolism (Vargas-Mendoza et al., 2014). Based on these reports, a decrease in serum levels of protein and albumin in the CP group of the present study may be due to liver dysfunction, and silymarin improved protein metabolism due to its hepatoprotective action.
In the present study, the serum concentration of uric acid was significantly higher in CP than in CM on days 21, 28, and 42. Uric acid is one of the serum parameters, which increases in hepatocellular damages (Zaefarian et al., 2019). In the current research, circulating uric acid significantly decreased in P500 and P2500 groups compared with the CP group that showed the protective effects of silymarin on liver health in broilers fed on a pellet diet.
In the current experiment, the activities of AST and ALT enzymes in serum significantly increased in CP compared to CM on day 42, and supplementary silymarin at dose 2500 ppm significantly decreased the serum activities of these enzymes in the P2500 group. The serum activity levels of AST and ALT are an indicator of liver damages in birds (Senanayake et al., 2015). Several studies have shown that ALT and AST increase in ascetic birds due to cardiac and hepatic damages (Arab et al., 2006; Fathi et al., 2015).
In the current study, supplementation with silymarin in birds fed on a pellet diet decreased the serum activities of ALT and AST enzymes, but this decrease was more significant in P2500. In agreement with these results, several studies have shown that silymarin has a hepatoprotective effect and decreases the serum AST and ALT activities in broilers with damaged liver (Fani Makki et al., 2014, Wang et al., 2018).
The protective effect of silymarin on the liver has been more related to antioxidant effects, which eliminates free radicals. In addition, this substance leads to increased RNA polymerase activity in the nucleus of hepatocytes and elevated ribosomal protein synthesis, which improves hepatic cell regeneration and can inhibit lipid peroxidation, reduce glutathione oxidation, and enhance liver detoxification (Moayedi Esfahani et al., 2015). Therefore, silymarin as a free radical chelating agent may be effective in hepatic disorders of RVF and ascites syndrome.
In this experiment, silymarin decreased serum concentrations of triglyceride, total cholesterol, LDL, and VLDL in M500, M2500, P500, and P2500 groups compared to control groups (CM and CP). Gobalakrishnan et al. (2016) and Wang et al. (2018) established that silymarin has a hypolipidemic effect.
Silymarin enhances hepatic bile acid synthesis and stimulates the excretion of bile acids to the intestine. Silymarin increases endogenous cholesterol conversion to bile acids and stimulates the production of hepatic LDL receptors, leading to increased clearance of plasma LDL, HDL, and indirectly that of VLDL (Gobalakrishnan et al., 2016). Also, silymarin was able to inhibit the biosynthesis of cholesterol in the liver and reduce LDL cholesterol oxidation (Wang et al., 2018).
Various studies have been performed on the hepatoprotective action of silymarin in birds, but there are few studies on the protective effect of silymarin on heart health. However, the cardioprotective activity of silymarin has been established on laboratory animals in several experiments (Rašković et al., 2011; Razavi & Karimi, 2016; Vilahur et al., 2018).
In the present research, serum CK activity increased significantly in the CP group compared to the CM group, and the activity of CK-MB decreased significantly in birds treated with 2500 ppm silymarin on day 42. The high serum activity levels of AST and CK are an indication of cardiac injuries in birds (Bodor, 2016). Several studies have shown that CK increased in ascetic birds due to cardiac damages (Arab et al., 2006; Fathi et al., 2015).
In the present study, the simultaneous increase in AST and CK in the CP group may be due to heart injury, which a high level of CK attenuated in the supplemented pellet group with silymarin. In the current research, circulating levels of calcium, phosphorus, and sodium were significantly lower in the CP group than in other groups on 28, 35, and 42 days. Also, birds in the CP group had lower circulating potassium and magnesium compared to the CM group, but it was not statistically significant on day 42.
Heart failure usually leads to acid-base and electrolyte disturbance due to the activation of several neurohumoral mechanisms. The most common electrolyte abnormalities in heart failure are hyponatremia, hypokalemia, and hypomagnesemia (Hanton et al., 2007). Silymarin at doses 500 and 2500 ppm increases the concentrations of calcium and phosphorus in P500 and P2500 groups compared to CP group. In the present experiment, silymarin at different doses had no improvement effect on electrolyte abnormalities in birds fed on a pellet diet. In agreement with obtained results, Lutensko et al. (2008) reported that silymarin increased serum levels of calcium and phosphorus.
In this study, T amplitude and T duration in the CM group were significantly lower compared to the CP group on day 42, and silymarin at dose 2500 ppm decreased significantly the duration and amplitude of the T wave in P2500 compared to CP and P500 groups. In the present study, supplementing a pelleted diet by silymarin at two different doses P500 and P2500 decreased traits of the T wave, but this effect was greater and significant in dose 2500 ppm (P2500 group). Several studies have shown that RVF in broilers changes the ECG waves’ pattern, especially in R, S, and T waves (Hassanpour et al., 2009; Yousefi et al., 2013; Sharifi et al., 2015). Hassanpour et al. (2005) stated that the duration of the T wave was enhanced in the broilers susceptible to pulmonary hypertension syndrome PHS and ascites.
In this study, the amplitude of the S wave significantly increased in CP compared with M500 and CM. The S-wave amplitude was significantly lower in P2500 than in CP on days 28 and 42. Previous studies have reported that an increase in the S amplitude reflected the hypertrophy of RVF and ascites (Hassanpour et al., 2009; Yousefi et al., 2013; Sharifi et al., 2015).
In this research, S duration on days 21 and 28 was shorter in the CP group than in CM, 500 and M2500 groups, and P22500had a significantly longer duration of the S wave 500, and M2500 on different days of the trial. In P500 and P2500, the ST segment was lower than in CP on all days.
In contrast to our research, Hassanpour et al. (2009) reported that the ST interval had no changes in broilers with RVF. In this research, the R-R interval in CP significantly decreased compared to CM. Also, P2 had a higher R-R interval than CP on day 42. The obtained results showed that HR significantly increased in CP compared to CM, and P2 had a lower HR than CP on day 42. Contrary to our results, Olkowski (2007) stated that HR decreased in broiler chickens susceptible to pulmonary hypertension and ascites syndrome. They showed that progressive bradycardia was seen in these birds due to circulatory insufficiency.
Our finding showed that feeding with a pellet diet changed some ECG parameters and serum activity of CK-MB enzyme in broilers, which can indicate heart disorders in birds fed on a pellet diet, and silymarin extract could modulate these changes. The main property of silymarin is the antioxidant activity of its flavonolignans and other polyphenolic constituents, which is attributable to free radical scavenging.
Silymarin leads to replenish endogenous antioxidant enzymes, suppress neutrophil infiltration, and reduce serum malondialdehyde as an end product of myocardial lipid peroxides (Moayedi Esfahani et al., 2015). Previous studies have stated that silymarin has cardioprotective effects in animal models due to lipid peroxidation inhibition and an increase in antioxidant enzymes production in heart tissue (Gabrielová et al., 2019).
Based on our results, silymarin had a protective effect on heart function and reversed ECG indices and serum biochemical changes in serum in fast-growing broilers. Thus, silymarin can be a clinical strategy to decrease the incidence of heart failure and ascites-induced mortality and has a financial benefit in the poultry industry.
In conclusion, it seems that using a pelleted diet in fast-growing broiler chickens could increase the susceptibility of broilers to liver and heart disorders and change some blood biochemical parameters and ECG values. Also, the present findings confirmed that silymarin had a dose-dependent protective effect on heart and liver health in broilers and attenuated induced negative changes using a pellet diet. Thus, silymarin can be used as a cardioprotective and hepatoprotective compound in broilers susceptible to ascites syndrome for decreasing ascites-induced mortality.
The authors are grateful to Shiraz University Shiraz, Iran, for financial supporting this study.
The authors reported no conflict of interest.
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