Physicochemical Parameters Affecting the In-vitro Toxins Production by Characterized Antibiotic-resistant Clostridium Perfringens Toxinotype B Isolates

Document Type : Original Articles

Authors

1 Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.

2 Institute of Biochemistry and Biotechnology, Faculty of Bioscience, University of Veterinary and Animal Sciences, Lahore, Pakistan.

10.32598/ijvm.19.3.1005561

Abstract

Background: Alpha, beta and epsilon toxins of Clostridium perfringens toxintype B are the major potent toxins involved in enterotoxemia. To combat this condition, proper vaccination of animals is required with an effective vaccine. 
Objectives: This research aimed to characterize the resistant C. perfringens type B isolates for in-vitro toxin production potential under the influence of various physicochemical parameters. 
Methods: C. perfringens isolates were characterized based on biochemical tests, toxinotyping, and 16S rRNA typing. The indigenously characterized C. perfringens toxinotype B isolates (n=6) were subjected to antibiotic susceptibility profiling through the Kirby-Bauer method. The resistant C. perfringens type B isolates were subjected to toxin production optimization under physicochemical parameters (physical: Temperature, pH and time of incubation; chemical: Glucose, vitamin-mineral mixture, tween 80 and sodium salts at various concentration). 
Results: The C. perfringens isolates were identified as toxinotype B. Isolates MW551887.1 and MW332247.1 produced higher hemolytic and cytotoxic units of toxins at a 0.2% glucose concentration in the broth after 24 hours at 37 ºC, respectively. 
Alpha toxin, Beta toxin, Clostridium perfringens toxinotype B, Epsilon toxin, Glucose: To combat disease, controlling antibiotic resistance and ensuring proper vaccination of animals is crucial. These C. perfringens isolates may have commercial applications for toxoid vaccine production after further. Conclusion: To combat disease, control of antibiotic resistance and proper vaccination of animals is crucial. These C. perfringens isolates may have commercial application for toxoid vaccine production after further characterization and molecular testing of toxins.

Keywords

Iranian Journal of Veterinary Medicine
Volume 19, Issue 3
July 2025
Pages 503-514

Files

Share

How to cite

Statistics