Effect of Protein Deficiency Diet on Gastric Histology and Histomorphometric Indices in Mice Animal Model

Articles in Press

Document Type : Original Articles

Authors

1 Anatomical Sciences and Cognitive Neurosciences Department, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran

Abstract

BACKGROUND: Diets in various organisms in the long run can affect the histological structure and histomorphometry of the gastrointestinal tract to adapt to the diet in order to be able to provide its main role in providing the necessary materials and energy for the survival of the organism.
OBJECTIVES: The aim of this study is to investigate the effect of dietary protein deficiency on histology and histomorphometric indices of stomach and blood chemical parameters and liver enzymes.
METHODS: Twelve immature female Balb/C mice at the age of three weeks were divided into a control group that received a complete protein diet and an experimental group that received a protein-deficient diet. Three months later, the serum levels of calcium, phosphorus, glucose, cholesterol, triglycerides, urea, creatinine, and liver enzymes were evaluated and the thickness of the layers of the stomach wall, as well as pit depth, the number of parietal cells was measured in stomach tissue.
RESULTS: The results of comparing the thickness of stomach layers showed that the thickness of the mucosa, muscle layer, depth of pits and the number of parietal cells of the stomach wall increased significantly in the experimental group(P<0.05). Also, the serum levels of phosphorus, glucose, cholesterol, triglyceride, urea, creatinine, AST and ALT in the experimental group showed a significant decrease, and the serum level of ALP in the experimental group showed a significant increase (P<0.05).
CONCLUSION: The results of the present study showed that changes in diet in the long term can cause changes in the histology and histomorphometry of the stomach wall as well as blood parameters, which may be unfavorable for people's health in some parameters.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 06 January 2025

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