Mito-TEMPO Protects Buck’s Semen Quality During Cooling Process

Articles in Press

Document Type : Original Articles

Authors

1 Department of Animal Science, College of Agriculture, Tabriz University, Tabriz, Iran

2 Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Peroxidation of membrane lipids is one of the most important chemical stresse in sperm, but utilization of antioxidants in semen diluent protect cells against oxidative damages and it could be a beneficial way to preserve semen quality during storage process. The current study was aimed to assess the effect of Mito-TEMPO on quality parameters of buck semen during cooling process. In this experiment, 5 Saanen bucks were used and semen samples were collected twice a week. After semen collection and primary evaluation, semen samples were diluted and assigned into five equal aliquots. Then samples were added to extenders containing 0, 1, 10, 100 and 1000 μM Mito-TEMPO. The prepared samples were stored at 5 °C up to 60 h. Chilled sperm motility, progressive motility, viability, mitochondrial activity, membrane functionality and malondialdehyde concentration were analyzed during 0, 30 and 60 h. In results at time 0, no difference was observed among groups but at 30 and 60 h storage, higher (P ≤ 0.05) sperm total motility, progressive motility, mitochondrial activity, membrane functionality, viability and lower lipid peroxidation were observed in 10 and 100 μM Mito-TEMPO groups than the others. In conclusion, supplementation of buck cooling medium with Mito-TEMPO as a novel mitochondria targeted antioxidant could be an impressive method to conserve of buck's semen quality during cooling storage process.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 01 February 2025

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