Mito-TEMPO Protects Buck’s Semen Quality During the Cooling Process

Document Type : Original Articles

Authors

1 Department of Animal Science, Faculty of Agriculture, Tabriz University, Tabriz, Iran.

2 Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

10.32598/ijvm.20.2.1005681

Abstract

Background: Membrane lipid peroxidation is one of the most significant chemical stresses in sperm. However, the utilization of antioxidants in semen diluent protects cells against oxidative damage, which could be a beneficial way to preserve semen quality during the storage process. 
Objectives: The current study aimed to assess the effect of Mito-TEMPO on quality parameters of buck semen during the cooling process. 
Methods: In this experiment, 5 Saanen bucks were used, and their semen samples were collected twice a week. After semen collection and primary evaluation, semen samples were diluted and assigned into 5 equal aliquots. Then, the samples were added to extenders containing 0, 1, 10, 100, and 1000 μM Mito-TEMPO (triphenylphosphonium chloride). The prepared samples were stored at 5 °C for up to 60 h. Chilled sperm motility, progressive motility (PM), viability, mitochondrial activity, membrane functionality, and malondialdehyde (MDA) concentration were analyzed during 0, 30, and 60 h. 
Results: In results at time 0, no difference was observed among groups, but at 30 and 60 h storage, higher (P≤0.05) sperm total motility (TM), PM, mitochondrial activity, membrane functionality, viability, and lower lipid peroxidation were observed in 10 and 100 μM Mito-TEMPO groups than the others.
Conclusion: In conclusion, supplementation of buck cooling medium with Mito-TEMPO as a novel mitochondria-targeted antioxidant could be an impressive method to conserve buck’s semen quality during the cooling storage process. 

Keywords

Iranian Journal of Veterinary Medicine
Volume 20, Issue 2
March and April 2026
Pages 321-328

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