Enhancing Antioxidant Status and Disease Resistance against Aeromonas hydrophila in Rainbow Trout, Oncorhynchus mykiss Through Dietary Ginseng Essential Oil Supplementation

Articles in Press

Document Type : Original Articles

Authors

1 Department of Veterinary Medicine, RUDN University, 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation

2 All-Russian Research Institute of Integrated Fish Farming - a branch of the Federal State Budgetary Scientific Institution "Federal Research Center for Animal Husbandry nameted after Academy Member L. K. Ernst" (L. K. Ernst

3 Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Golestan, Iran

10.22059/ijvm.2024.385103.1005676

Abstract

Background: Disease outbreaks pose a significant threat to the aquaculture industry. The use of supplementary feeds is especially popular among fish farmers to improve their overall health and overcome disease.
Objectives: This study aimed to evaluate the effects of dietary ginseng, Panax ginseng, essential oil (GE) on immunological and antioxidant parameters, and disease resistance of rainbow trout, Oncorhynchus mykiss.
Methods: Fish were fed diets containing 0 ml/kg (control; CTL), 0.5 ml/kg (GE0.5), 1.0 ml/kg (GE1.0), 1.5 ml/kg (GE1.5), 2.0 ml/kg (GE2.0), 2.5 ml/kg (GE2.5), 3.0 ml/kg (GE3.0), and 3.5 ml/kg (GE3.5) GE for 8 weeks, in triplicate, then experimentally infected with Aeromonas hydrophila.
Results: Results indicated that the GE3.0 treatment demonstrated the highest levels of serum superoxide dismutase, catalase, and glutathione peroxidase, while the lowest serum malondialdehyde levels were recorded in the GE2.0 and GE2.5 groups. Furthermore, the GE3.0 treatment resulted in the lowest serum levels of alanine aminotransferase and lactate dehydrogenase, whereas the GE2.0 treatment showed reduced serum alkaline phosphatase and aspartate aminotransferase levels. Immunological assessments revealed that the GE3.0 treatment had the highest serum and mucus lysozyme and immunoglobulin levels, along with elevated serum alternative complement, myeloperoxidase, and mucus protease activity. Blood respiratory burst activity and mucus alkaline phosphatase were also highest in the GE2.5 and GE3.0 treatments. Notably, the lowest post-infection mortality rate was observed in the GE3.0 group.
Conclusion: Based on these findings, it is recommended to include 2-3 ml/kg of GE in trout feed to enhance fish health and disease resistance.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 05 May 2025

Files

Share

How to cite

Statistics