Document Type : Original Articles
Authors
1
Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
2
Department of Physiology; Biochemistry; and Pharmacology College of Veterinary Medicine; University of Mosul; Mosul ; Iraq
10.22059/ijvm.2025.389558.1005738
Abstract
Background: Nowadays, there is a need to discover the potential impact of oxidative stress (OS) involving a drug response to be warranted and applied in veterinary medicine.
Objective: We aimed to evaluate the effect of hydrogen peroxide-induced OS on the pharmacological efficacy of nimesulide (NIM) in chickens.
Methods: Assessing the NIM median effective dose (ED50) by using the up-and-down method, cyclooxygenase2 (COX2) activity via ELISA technique, spectrophotometric analysis of NIM concentrations and its pharmacokinetics.
Results: Stressed chickens showed a significant (p< 0.05) reduction in the total antioxidant capacity (T-AOC) at 7th, 10th, and 14th days of hydrogen peroxide treatment by 46, 58, and 22%, correspondingly in comparison to the control (tap water). The NIM antinociceptive efficacy was increased due to lowering ED50 by 31% in the stressed chickens. Consequently, there was a significant (p< 0.05) inhibition in COX2 activity both stressed and control chickens by 24 and 30%, respectively. The data also showed a significant (p< 0.05) modification in the NIM plasma concentration in the stressed chickens in contrast to the control one at different measurement times. As for the NIM pharmacokinetic parameters, OS causes an elevation of the AUC 0-∞, AUMC 0-∞, Cmax, MRT, t 1/2β and Vss by 11, 42, 11, 35, 46, and 39%, respectively while Kel and Cl were decreased by 45 and 8%, respectively.
Conclusion: These results showed that OS modifies the pharmacodynamics and pharmacokinetics of NIM which modifies its pharmacological efficacy; care and adjustment of the NIM dosage warrant its use in stressed animals.
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