Investigation of Some Prognostic Biomarkers in Autoimmune Diseases in Laboratory Mice Exposed to Polystyrene Microplastics

Articles in Press

Document Type : Original Articles

Authors

1 Department of comparative bioscience, faculty of veterinary medicine, university of Tehran, Tehran, Iran

2 Toxicology dep. Faculty of Veterinary Medicine, University of Tehran

3 Rastegar Reference Laboratory, School of Veterinary Medicine, University of Tehran, Tehran, Iran

4 Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Background: Plastic pollution has emerged as a significant environmental hazard, with microplastics posing potential risks to both human and animal health

Objectives: This study aimed to evaluate the effects of polystyrene microplastics on the immune system, inflammation, autoantibody production, and autoimmune diseases, as well as changes in hematological and biochemical parameters in laboratory mice.

Methods: Twenty-four male NMRI mice (25-35 g) and 16 weeks old were randomly divided into four groups and exposed to 0.1 mL of polystyrene microplastics (2 µm diameter, 1 mg/kg) via gavage for 40 and 60 consecutive days. Blood samples were collected for complete blood count (CBC), sedimentation rate, biochemical (AST, ALT, BUN, creatinine, uric acid), and immunological (indirect immunofluorescence for autoantibodies) analyses.

Result: Polystyrene microplastics induced autoantibodies in serum at a dilution of 1:40 after 60 days. Mice developed microcytic hypochromic anemia after 40 and 60 days of exposure to polystyrene microplastics.

White blood cell (WBC) counts increased after 40 days but decreased after 60 days compared to the control group. Platelet counts increased significantly after 40 and 60 days. Sedimentation rates increased in exposed mice after 40 and 60 days.

Conclusions:

The findings suggest that exposure to polystyrene microplastics for 60 days may induce autoimmune-like disease in mice. Changes in hematological parameters, as well as increased sedimentation in mice and positive autoantibody titers in mice exposed to microplastics for 60 days, could be the result of inflammation or the development of an autoimmune-like disease

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 02 December 2025

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