Nanocurcumin Impact on the Expression of bap Gene

Articles in Press

Document Type : Original Articles

Authors

1 Diyala province, Iraq

2 .Department of Biology, Faculty of education for pure sciences, University of Diyala, Diyala, Iraq.

3 Department of Biology, Faculty of sciences, University of Diyala , Diyala, Iraq.

Abstract

Background : Acinetobacter baumannii is an opportunistic pathogen known for its multidrug resistance and strong ability to form biofilms, which significantly contributes to its persistence in clinical environments.
Objectives: This research aimed to assess the impact of nano-curcumin onto both the antibacterial efficacy and biofilm forming capacity for clinical isolates multidrug-resistant A. baumannii, and to assess the level of expression for bap gene related to the production of biofilms.
Methods: the effectiveness of nano curcumin was evaluated in vitro on 19 clinical isolates MDR A. baumannii. the activity of nano-curcumin for preventing A. baumannii biofilm was evaluated using 96-well micro-titer plates. The antibacterial efficacy was assessed by using the broth micro-dilution technique and by estimating MIC. The kirby-bauer disc diffusion method assessed the susceptibility of isolates for different antibiotics.The micro-dilution broth technique determined the mic of nano curcumin. the expression of the bap gene was assessed utilizing qRT-PCR. outside the ten isolates of A baumannii MDR, there can be a strong biofilms producer.
Results: Maximum resistance was observed against amoxicillin–clavulanic acid and ceftazidime, with all 19 isolates (100%) showing resistance to both antibiotics. In contrast, minimum resistance was recorded for meropenem, with only four isolates (21.05%) exhibiting resistance.
Ten isolates demonstrated a significant capacity for biofilm formation. The minimum inhibitory concentration (MIC) of nanocurcumin was 128 µg/mL for all ten biofilm-forming isolates. Furthermore, treatment with nanocurcumin resulted in a decrease of bap gene expression to 0.35-fold compared with untreated samples.
Conclusion: These results indicate that nanocurcumin may serve as a potential agent for reducing the development of Acinetobacter biofilms. The observed decreased levels of the bap gene further corroborate the anti-biofilm efficacy of nanocurcumin.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 19 May 2026

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