Background: The use of IgYs in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. Objectives: Development of antibodies against extra cellular domain of influenza M2 (M2e) protein in egg yolk of laying hens. Methods: A Fusion construct harboring C-terminal of bovine heat shock protein 70 (Hsp70) and influenza M2e coding genes was injected to laying hens. Serum and egg yolk antibodies were screened for the presence of anti-M2e antibodies by indirect enzyme-linked immunosorbent assay (ELISA). Results: Anti-M2e antibodies were detected in egg yolks and sera of injected hens from 13 and 7 days post injection (PI), with the peak titer detected on 41 and 35 days PI, respectively. Conclusions: Anti-M2e IgY titers could be an index for expression potential of pcDNA3.1-M2e-HspC-terminal construct in laying hens. This construct could be considered as a promising tool in production of anti-M2e polyclonal, monospecific IgY antibodies. Such anti-M2e antibodies could be exploited for influenza diagnostic and therapeutic measures.
نیکبخت بروجنی, غ. , Nikbakht, G. R. , Tajbakhsh, H. , Jahantigh, M. , Niazi Shahraki, S. and Madadgar, O. (2012). Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene. Iranian Journal of Veterinary Medicine, 6(2), 67-71. doi: 10.22059/ijvm.2012.28949
MLA
نیکبخت بروجنی, غ. , , Nikbakht, G. R. , , Tajbakhsh, H. , , Jahantigh, M. , , Niazi Shahraki, S. , and Madadgar, O. . "Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene", Iranian Journal of Veterinary Medicine, 6, 2, 2012, 67-71. doi: 10.22059/ijvm.2012.28949
HARVARD
نیکبخت بروجنی, غ., Nikbakht, G. R., Tajbakhsh, H., Jahantigh, M., Niazi Shahraki, S., Madadgar, O. (2012). 'Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene', Iranian Journal of Veterinary Medicine, 6(2), pp. 67-71. doi: 10.22059/ijvm.2012.28949
CHICAGO
غ. نیکبخت بروجنی , G. R. Nikbakht , H. Tajbakhsh , M. Jahantigh , S. Niazi Shahraki and O. Madadgar, "Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene," Iranian Journal of Veterinary Medicine, 6 2 (2012): 67-71, doi: 10.22059/ijvm.2012.28949
VANCOUVER
نیکبخت بروجنی, غ., Nikbakht, G. R., Tajbakhsh, H., Jahantigh, M., Niazi Shahraki, S., Madadgar, O. Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene. Iranian Journal of Veterinary Medicine, 2012; 6(2): 67-71. doi: 10.22059/ijvm.2012.28949
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