Iranian Journal of Veterinary Medicine

Iranian Journal of Veterinary Medicine

Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene

Authors
غلامرضا نیکبخت بروجنی 1
Gholam Reza Nikbakht 1
Hasan Tajbakhsh 2
Monireh Jahantigh 1
Siavash Niazi Shahraki 3
Omid Madadgar 1
1 گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه تهران
2 استاد ممتاز دانشگاه تهران و عضو پیوسته فرهنگستان علوم
3 آزمایشگاه مرکز تشخیص سازمان دامپزشکی ایران
10.22059/ijvm.2012.28949
Abstract
Background: The use of IgYs in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. Objectives: Development of antibodies against extra cellular domain of influenza M2 (M2e) protein in egg yolk of laying hens. Methods: A Fusion construct harboring C-terminal of bovine heat shock protein 70 (Hsp70) and influenza M2e coding genes was injected to laying hens. Serum and egg yolk antibodies were screened for the presence of anti-M2e antibodies by indirect enzyme-linked immunosorbent assay (ELISA). Results: Anti-M2e antibodies were detected in egg yolks and sera of injected hens from 13 and 7 days post injection (PI), with the peak titer detected on 41 and 35 days PI, respectively. Conclusions: Anti-M2e IgY titers could be an index for expression potential of pcDNA3.1-M2e-HspC-terminal construct in laying hens. This construct could be considered as a promising tool in production of anti-M2e polyclonal, monospecific IgY antibodies. Such anti-M2e antibodies could be exploited for influenza diagnostic and therapeutic measures.
Keywords
e2M
Iranian Journal of Veterinary Medicine
Volume 6, Issue 2 - Serial Number 1177763
15 Iranian Journal Of Veterinary Medicine
May and June 2012
Pages 67-71