Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line
BACKGROUND: Some of metal ions as environmental pollutants show estrogenic activity. This xenostrogenic compounds can be caused carcinogenicity in organs. The mechanism of carcinogenicity of metal ions is not clarified. OBJECTIVES: In this study, we investigated the Transcriptional effects of variety of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter by estrogen receptor a in MDA-MB 231 breast cancer cell line. METHODS: Cells were plated into flask (75cm2) at 1.3 density or into 12- well plates (Nunc) at a density of 100000 cells per well and were transfected with a total of 3 µg of plasmid DNA using calcium phosphate coprecipitation. Oestrogen and some metal ions were used for stimulation of transfected cells. RESULTS: Our results showed that copper and cadmium ions activating specifically the oxytocin promoter, and cobalt and possibly, mercury ions activating specifically the ERE-controlled promoter and the majority of the ions did not affect transcriptional activation significantly. CONCLUSIONS: The study revealed that some metal ions show estrogenic activity by classical or non-classical mechanisms as well as some metal ions exhibit estrogenic activity by undetermined mechanisms in transfected MDA-MB 231 cell line.
کوهی, م. ک. , Zayerzadeh, E. , Eslami, M. and Zadeh Hashem, E. (2012). Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line. Iranian Journal of Veterinary Medicine, 6(2), 79-87. doi: 10.22059/ijvm.2012.28951
MLA
کوهی, م. ک. , , Zayerzadeh, E. , , Eslami, M. , and Zadeh Hashem, E. . "Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line", Iranian Journal of Veterinary Medicine, 6, 2, 2012, 79-87. doi: 10.22059/ijvm.2012.28951
HARVARD
کوهی, م. ک., Zayerzadeh, E., Eslami, M., Zadeh Hashem, E. (2012). 'Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line', Iranian Journal of Veterinary Medicine, 6(2), pp. 79-87. doi: 10.22059/ijvm.2012.28951
CHICAGO
م. ک. کوهی , E. Zayerzadeh , M. Eslami and E. Zadeh Hashem, "Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line," Iranian Journal of Veterinary Medicine, 6 2 (2012): 79-87, doi: 10.22059/ijvm.2012.28951
VANCOUVER
کوهی, م. ک., Zayerzadeh, E., Eslami, M., Zadeh Hashem, E. Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line. Iranian Journal of Veterinary Medicine, 2012; 6(2): 79-87. doi: 10.22059/ijvm.2012.28951
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