Effects of mesenchymal stem cells with injectable scaffold on cardiac function in myocardial infarction in Rabbit

Jafari, Nazanin; Dehghan, Mohammad Mahdi; Abarkar, Mohammad; Hejazi, Mohammad; Abbasnia, Pegah; Molazem, Mohammad; Tavakoli, Amir; Mehdinavaz Aghdam, Rouh-allah; Ahmadi Tafti, Seyed Hosein; Tajik, Parviz

doi:10.22059/ijvm.2013.32018

Effects of mesenchymal stem cells with injectable scaffold on cardiac function in myocardial infarction in Rabbit

Authors

Nazanin Jafari ¹

Mohammad Mahdi Dehghan ¹

Mohammad Abarkar ²

Mohammad Hejazi ³

Pegah Abbasnia ¹

Mohammad Molazem ¹

Amir Tavakoli ¹

Rouh-allah Mehdinavaz Aghdam ⁴

Seyed Hosein Ahmadi Tafti ⁵

Parviz Tajik ¹

¹ Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

² Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran

³ Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University, Khorramabad Branch, Khorramabad, Iran

⁴ Nanotechnology Department, Engineering Research Institute, Tehran, Iran

⁵ Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran

10.22059/ijvm.2013.32018

Abstract

BACKGROUND: Bone marrow-derived mesenchymal cells
can transdifferentiate into Cardiomyocyte cells and improve
heart function after transplantation. Since biomaterials can
improve the cell retention in the site, cell survival and differentiation,
heart tissue engineering is now being explored as an
applied solution to support cell-based therapies and increase
their efficacy for myocardial diseases. Chitosan in combination
with Glycerol Phosphate (GP) can produce a thermo sensitive
material that in body temperature can form a jellylike material.
OBJECTIVES:The aim of this study was to evaluate the effects of
a combination of autologous undifferentiated bone marrow
mesenchymal stem cells (MSCs) and injectable scaffold on cardiac
function improvement in rabbits after inducing myocardial
infarction. METHODS: The Left Anterior Descending (LAD)
coronary artery was ligated by No. 6-0 polyamide suture
material, and autologous MSCs with injectable scaffold were
injected into the margins of the infarcted zone at the time of
surgery. At 4 weeks after transplantation, the cardiac function
and structure was detected using echocardiography. RESULTS:
There was no significant difference among the three groups (MI
only, MI Scaffold, and MI+Scaffold+MSCs) in the Echocardiographic
parameters including, heart rate (HR), Ejection Fraction
(EF), Fractional Shortening (FS), Left Ventricular Diameter
(LVD) and Left Ventricular Parietal Wall Diameter (LVPW).
CONCLUSIONS: A combination of autologous undifferentiated
bone marrow MSCs and injectable scaffold made of Chitosan+
Glycerol Phosphate in echocardiographic evaluation did not
have a positive influence on achieving functional improvement.

Keywords

Echocardiography

mesenchymal stem cell

Scaffold

ejection fraction

fractional shortening