Effects of mesenchymal stem cells with injectable scaffold on cardiac function in myocardial infarction in Rabbit


1 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran

3 Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University, Khorramabad Branch, Khorramabad, Iran

4 Nanotechnology Department, Engineering Research Institute, Tehran, Iran

5 Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran


BACKGROUND: Bone marrow-derived mesenchymal cells
can transdifferentiate into Cardiomyocyte cells and improve
heart function after transplantation. Since biomaterials can
improve the cell retention in the site, cell survival and differentiation,
heart tissue engineering is now being explored as an
applied solution to support cell-based therapies and increase
their efficacy for myocardial diseases. Chitosan in combination
with Glycerol Phosphate (GP) can produce a thermo sensitive
material that in body temperature can form a jellylike material.
OBJECTIVES:The aim of this study was to evaluate the effects of
a combination of autologous undifferentiated bone marrow
mesenchymal stem cells (MSCs) and injectable scaffold on cardiac
function improvement in rabbits after inducing myocardial
infarction. METHODS: The Left Anterior Descending (LAD)
coronary artery was ligated by No. 6-0 polyamide suture
material, and autologous MSCs with injectable scaffold were
injected into the margins of the infarcted zone at the time of
surgery. At 4 weeks after transplantation, the cardiac function
and structure was detected using echocardiography. RESULTS:
There was no significant difference among the three groups (MI
only, MI Scaffold, and MI+Scaffold+MSCs) in the Echocardiographic
parameters including, heart rate (HR), Ejection Fraction
(EF), Fractional Shortening (FS), Left Ventricular Diameter
(LVD) and Left Ventricular Parietal Wall Diameter (LVPW).
CONCLUSIONS: A combination of autologous undifferentiated
bone marrow MSCs and injectable scaffold made of Chitosan+
Glycerol Phosphate in echocardiographic evaluation did not
have a positive influence on achieving functional improvement.