PCR-based detection of cow and goat milk in sheep milk and dairy products marketed in Mashhad city of Iran

Khanzadi, Saeed; Jamshidi, Abdollah; Razmyar, Jamshid; Mohsenzadeh, Mohammad

doi:10.22059/ijvm.2013.36285

PCR-based detection of cow and goat milk in sheep milk and dairy products marketed in Mashhad city of Iran

Authors

Saeed Khanzadi ¹

Abdollah Jamshidi ¹

Jamshid Razmyar ²

Mohammad Mohsenzadeh ¹

¹ Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

² Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

10.22059/ijvm.2013.36285

Abstract

BACKGROUND: The extensive consumption of milk and
dairy products makes these foodstuffs targets for potential
adulteration with financial gains for unscrupulous producers.
OBJECTIVES: The aim of this study was using PCR assay to
detect cow milk in labeled sheep milk, sheep yoghurt, and
Lighvan cheese (a traditional ripened cheese produced from
sheep's milk). METHODS: The assay utilized primers targeting
the mitochondrial 12s and 16s rRNA gene. In this study, 35
samples of sheep milk, 35 samples of sheep yoghurt, and 35
samples of Lighvan cheese were purchased from different
supermarkets in Mashhad city with different batch numbers.
RESULTS: The results showed only 21 out of 105 (20%) samples
contained pure sheep milk. Undeclared presence of cow and goat
milk was detected in 33(31.5%) and 68(65%) of the 105 samples,
respectively. CONCLUSIONS: It seems the PCR based analytical
method is an applicable technique to monitor adulteration in
dairy products.

Keywords

Adulteration

Lighvan cheese

Milk

PCR-based method

yoghurt