Detection of gene expression and sequence analysis of chicken class II trans activator (CIITA)

Authors

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

BACKGROUND:Class II transactivator (CIITA) is a dominant
transcriptional element, controlling numerous genes in the
immune system. CIITA is expressed in a constitutive pattern in
antigen presenting cells although its expression can occur in
other cell types. Since the revelation of CIITA, there have been
considerable advances toward understanding its role as an
activator of MHC II genes in humans and mice; nonetheless,
there is a lack of published data for this gene in other animals such
as chickens. OBJECTIVES: The goals of this study were to
determine the expression of class II transactivator (CIITA) in
chicken and analysis of the CIITA gene sequence between four
Iranian indigenous chicken ecotypes. METHODS:After securing
the research accuracy and optimization of reaction conditions,
cDNA and DNA samples of gene were obtained from four
Iranian indigenous chicken ecotypes. The PCR and RT-PCR
products were sequenced and the data were analyzed by
bioinformatics software. RESULTS: Comparison of the
sequencing results with the reference sequence of the red jungle
fowl revealed that these sequences belonged to the predicted
CIITA gene. There was a high conservation rate in the sequence
of CIITA. CONCLUSIONS: Our results indicated that like other
species, CIITA is transcripted in chickens' immune system cells.
Further studies on chickens must be done to reveal CIITAroles in
immune responses of chickens.

Keywords

Iranian Journal of Veterinary Medicine
Volume 7, Issue 4 - Serial Number 4
January 2014
Pages 287-292

Files

Share

How to cite

Statistics