Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012

Authors

1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran

3 Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

4 Department of Research & Production of Veterinary and Poultry Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran

5 Department of Poultry Health and Diseases, Iranian Veterinary Organization, Tehran, Iran

6 Students' Scientific Association of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

BACKGROUND: Newcastle disease virus (NDV) is the causative
agent of the Newcastle disease (ND), a highly contagious
disease in birds that causes significant economic losses to the
poultry industry worldwide. ND is endemic in Iran and outbreaks
are reported regularly in commercial poultry flocks and different
species of birds. OBJECTIVES:The current study was carried out
to characterize NDV based on phosphorprotein (P) gene from
recent outbreaks in Iran, 2010-2012. METHODS: The P gene
fragment of NDV isolates of five chickens, 1 ostrich, and 1
Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced.
RESULTS: Phylogenetic analysis of sequences revealed that
chicken and ostrich NDV isolates were closely related and
placed in the genotype VII and Pigeon Paramyxovirus-1 was
located in the genotype V. CONCLUSIONS:This is the first report
of Phosphoprotein gene sequences of NDV strains isolated in
Iran. This study will help us to understand the epidemiology and
molecular characteristics of Newcastle disease virus in Iran.

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