A study of hematological changes in sheep naturally infected with Anaplasma spp. and Theileria ovis: Molecular diagnosis

Document Type : Clinical Pathology

Authors

1 Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

BACKGROUND: Ovine anaplasmosis and theileriosis are important tick-borne diseases of sheep and goats which are distributed in the tropical and subtropical areas of the world. OBJECTIVES: This study was performed to assess hematological status in sheep naturally infected with Anaplasma and Theileria spp. to clarify the pathogenic aspects of various species involved in ovine anaplasmosis and theileriosis in Ahvaz region. METHODS: 109 sheep were sampled, and blood parasite infections were diagnosed by microscopic examination and PCR. The blood samples were also subjected to hematologic assessment. RESULTS: PCR analysis revealed A. ovis infection in 86.2% of sheep, while mixed infections with A. marginale were also detected in 53.2% of them. However, Anaplasma inclusion bodies were only observed in 32.1% of the tested animals. T. ovis were found in 88% of the inspected sheep by PCR, and 67.8% of them were detected microscopically, as well. Hematologic assessment showed that mean RBC, PCV, Hb, and MCHC were significantly lower, whereas MCV and RDW were higher in the animals with mixed infections of Anaplasma with parasitemia and Theileria, compared to the uninfected sheep and groups with single infection or without parasitemia. CONCLUSIONS: In brief, it seems that Anaplasma can be activated and induce its pathogenesis in the presence of other infective agents in the carrier or asympthomatic animals. It can also be concluded that mixed infections of Anaplasma with parasitemia and Theileria may induce a regenerative anemia which is most likely attributable to a combined effect of the two.

Keywords

Article Title [Persian]

مطالعه تغییرات خون شناسی در گوسفندان آلوده به آناپلاسما و تیلریا اویس: با تشخیص مولکولی

Authors [Persian]

  • زهره خاکی 1
  • سیده میثاق جلالی 2
  • بهرام کاظمی 3
  • محمد راضی جلالی 2
  • سیده پرستو یاسینی 4
1 بخش کلینیکال پاتولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
2 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
3 مرکز تحقیقات بیولوژی سلولی و مولکولی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران
4 بخش کلینیکال پاتولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
Abstract [Persian]

زمینه مطالعه: آناپلاسموز و تیلریوز بیماریهای مهم منتقله از کنه در گوسفند و بز هستند که در مناطق گرمسیری و نیمه گرمسیری دنیا منتشر هستند. هدف: مطالعه حاضر به منظور بررسی تغییرات خون شناسی در گوسفندان دچار آلودگی طبیعی به آناپلاسما و تیلریا انجام گرفت تا جنبه‌های پاتوژنیک گونه‌های مختلف ایجاد کننده آناپلاسموز و تیلریوز گوسفند در اهواز مشخص گردد. روش کار: نمونه‌گیری از 109 گوسفند انجام گرفت و آلودگی انگل‌های خونی با بررسی میکروسکوپی و PCR تشخیص داده شد. نمونه‌های خون همچنین از نظر خون شناسی مورد ارزیابی قرار گرفت. نتایج: در آزمایش PCR آلودگی به آناپلاسما اویس در 2/86% از گوسفندان مشخص شد در حالی که در 2/53 % از این موارد، آلودگی همزمان به آناپلاسما مارژیناله نیز یافت شد. با این وجود گنجیدگی‌های آناپلاسما تنها در 1/32% از حیوانات مورد بررسی مشاهده گردید. تیلریا اویس در 88 % از گوسفندان مورد بررسی به روش PCR شناسایی گردید که در 8/67 % از این موارد آلودگی به روش میکروسکوپی نیز تشخیص داده شد. بررسی‌های خون شناسی نشان داد که در حیوانات دچار آلودگی همزمان به آناپلاسما همراه پارازیتمی و تیلریا، میانگین RBC، PCV، Hb و MCHC به طور معنی داری کمتر، در حالی که MCV و RDW بیشتر از گوسفندان غیرآلوده و دام‌های دچار آلودگی تکی یا بدون پارازیتمی بود. نتیجه‌گیری‌نهایی: به طور خلاصه، به نظر می‌رسد که آناپلاسما می‌تواند در حضور سایر عوامل عفونی در حیوانات حامل یا فاقد علامت، فعال شده و بیماریزایی ایجاد کند. همچنین آلودگی‌های همزمان آناپلاسما همراه پارازیتمی و تیلریا می‌تواند یک کم خونی جبران پذیر ایجاد کند که احتمالاً مرتبط با اثر هر دو انگل می‌باشد.

Keywords [Persian]

  • آناپلاسما
  • تیلریا
  • گوسفند
  • خون شناسی

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