The comparison of “tim” gene of Giardia lamblia in laboratory animals and human and the importance of cross transmission probability in Iran

Authors

1 Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran. Zoonosis Research Center (ZRC),Tehran University of Medical Sciences, Tehran, Iran

2 Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

3 Department of Medical Parasitology and Mycology, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences.

Abstract

BACKGROUND: Giardia has the ability to infect many mammals including dogs, cats, deer, mice, ground squirrels, chinchillas, swine, rabbits, pocket mice, oxen, guinea pigs, and humans. Giardia lamblia (also Giardia duodenalis, G.intestinalis) isolates have been variably divided into two or three genotypes by different investigators, and each group can be divided into subgroups. OBJECTIVES: We have compared the triosephosphate isomerase (tpi) sequences of these genotypes by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) to determine G.lambia genotype in Iran for the first time. METHODS: In this study, 4 sets of primers were used in which 2 sets were designed by other investigator, and 2 sets were designed by the authors of the present study to confirm the results of the first two primers and also to differentiate the subgroups. RESULTS: Among Giardia isolates, 2/10 and1/19 of PCR-RFLP of rabbit and mouse respectively amplified with primer PM290. CONCLUSIONS: There is evidence that suggests that direct transmission from companion animals to human does occur. Zoonosis is controversial regarding Giardia; however, most researchers believe that its zoonotic potential merits adequate precaution when working with feces of animals that may be infected.

Keywords


Article Title [فارسی]

مقایسه ژن «tim» ژیاردیا لامبلیا در حیوانات آزمایشگاهی و انسان و اهمیت انتقال متقاطع در ایران

Authors [فارسی]

  • میترا زارع بوانی 1
  • ناهید عین الهی 2
  • نسرین دشتی 2
  • مصطفی رضائیان 3
1 مرکز تحقیقات سمشناسی و مسمومیتهای دامی، گروه سمشناسی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران. مرکز تحقیقات زئونوز، دانشگاه علوم پزشکی تهران، تهران، ایران
2 مرکز تحقیقات سمشناسی و مسمومیتهای دامی، گروه سمشناسی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
3 گروه طب داخلی، دانشگاه گرونینگن، گرونینگن، هلند
Abstract [فارسی]

زمینه مطالعه: انگل تک یاخته ژیاردیا توانایی آلوده کردن پستاندارانی از قبیل سگ، گربه، موش، سنجاب، چین چیلا، خرگوش، موش کوچک، خوکچه هندی، خوک، گاو و انسان را دارا می‌باشد. هدف: در این مطالعه برای اولین بار ژنوتیپ‌های ژن تریوزفسفات ایزومراز ژیاردیا لامبلیا به روش PCR-RFLP مورد مطالعه قرار گرفت. روش کار: در این بررسی 4 جفت پرایمر مورد استفاده قرار گرفت.  2 جفت پرایمر بر اساس مطالعات پیشین و 2 جفت دیگر طی مطالعه جهت افتراق زیر گروه‌ها طراحی گردید. نتایج: در میان ایزوله‌های ژیاردیای بررسی شده در این نحقیق، به ترتیب 20% و 5% از نمونه‌های  ژیاردیا با منشأ خرگوش و موش توسط پرایمر PM290 تکثیر گردید. نتیجه‌گیری‌نهایی: یافته فوق حاکی از وچود شباهت توالی درژن مذکور در بین نمونه انسانی، خرگوش و موش می‌باشد. که می‌تواند مبین وجود شواهدی مبتنی بر انتقال مستقیم ژیاردیا از حیوانات ذکر شده به انسان باشد و اکثر محققین بر این باورند که با توجه به پتانسیل انتقال زئونوتیک هنگام کار با مدفوع حیوانات آلوده بایستی دقت لازم را بعمل آورد.

Keywords [فارسی]

  • ژیاردیا لامبلیا
  • موش
  • PCR-RFLP
  • خرگوش
  • حیوانات آزمایشگاهی
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