Detection of cytolethal distending toxin (cdt) genes of Campylobacter Jejuni and Coli in fecal samples of pet birds in Iran

Authors

Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

BACKGROUND: Campylobacteriosis is one of the most common
bacterial intestinal disorders of humans in many countries.
A wide range of domestic and wild bird species have been identified
as natural reservoirs. OBJECTIVES: The aim of this study
was to determine the Campylobacter spp from pet birds and
screen the determined Campylobacters for presence of virulence
cytolethal distending toxin (cdt) genes. METHODS: A total
of 660 fecal samples from 32 different species of pet birds were
taken and examined for detection of Campylobacter spp. and
were investigated for presence of cdt genes. All the samples
were collected from clinically healthy birds that were kept in
cage, zoological parks, and/ or in zoo of Tehran, the capital of
Iran. RESULTS: In total, 20 thermophilic Campylobacter were
detected from 8 different avian species. From 20 confirmed
Campylobacter spp., 16 samples (80%) were C. jejuni positive
and 4 (20%) were C. coli in species-specific PCR test. Furthermore,
out of 20 detected Campylobacter, 13 (65%) harbored
the various subunits of cdtA, cdtB and cdtC genes, and 7 (35%)
were negative for all tested cdt genes. CONCLUSIONS: Our findings
indicate that the carriage rate of Campylobacter in different
species of cage and/or in zoo birds is high and confirm that
cdt genes may frequently be present in campylobacter spp.

Keywords


Article Title [فارسی]

شناسایی ژنهای حدت cdt کمپیلوباکترهای ژوژنی و کلی از نمونههای مدفوعی پرندگان خانگی (زینتی) ایران

Authors [فارسی]

  • فاطمه احسان نژاد
  • ارژنگ شیخ الملوکی
  • محمد حسن زاده
  • روجا شجاعی کاوان
  • محمد سلطانی
گروه بیماریهای پرندگان، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
Abstract [فارسی]

زمینه مطالعه: کمپیلوباکتر‌ها در بسیاری از موارد سبب آنتریت انسانی می‌شوند. تعداد زیادی از پرندگان اهلی و وحشی مخزن کمپیلوباکترها بوده و دارای ژن‌های حدت cdt می‌باشند. هدف: در این مطالعه ابتدا کمپیلوباکترهای ژژونی و کلی در پرندگان خانگی شناسایی و سپس حضور ژن‌های cdt در آنها مورد مطالعه قرار گرفت. روش کار: تعداد 660 نمونه سواب مدفوعی، از 32 گونه مختلف پرنده خانگی یا زینتی که به کلینیک دانشکده دامپزشکی مراجعه یا در قفس‌های پارک‌ها و باغ وحش تهران نگهداری می‌شدند تهیه گردید. با استفاده از روش باکتریولوژیک و PCR ابتدا کمپیلوباکترها شناسایی و سپس ژن‌های cdt آنها مطالعه گردید. ضمناً محصولات PCR برای سکانس ژن ارسال گردید. نتایج: تعداد 20 نمونه کمپیلوباکتر شامل 16 نمونه ژژونی (80%) و 4 نمونه کلی (20%) از 8 گونه مختلف پرنده خانگی جدا و یا با روش PCR شناسایی و تأیید گردید. در 20 نمونه مثبت، تعداد 13 نمونه (65%) حداقل دارای یک یا چند ژن حدت cdt بودند و 7 نمونه از آنها هیچ یک از ژن‌های cdt را در مطالعه مولکولی نشان ندادند. نتایج اولیه سکانس ژن‌های حدت موید این بود که قرابت بالایی بین ژن‌های حدت این باکتری‌ها با ژن‌های حاصل از جدایه‌های انسانی و طیور صنعتی وجود دارد.  نتیجه‌گیری‌نهایی: مطالعه حاضر نشان داد که پرندگان خانگی آلوده به کمپیلوباکتر بوده و حاوی ژن‌های حدت هستند و می‌توانند بعنوان مخزن باکتری محسوب و سبب آلودگی سایر پرندگان و انسان‌ها بشوند.

Keywords [فارسی]

  • کمپیلوباکتر گرمادوست
  • ژن حدت
  • پرندگان زینتی
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