Study on serum glucose, insulin, NEFA, BHBA and lipid profile in different productive status of high producing Holstein dairy cows

Document Type : Nutrition - Hygiene

Authors

Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

Background: Metabolic profiles have been used in efforts to predict pre parturient problems and fertility, to diagnose metabolic diseases, and to assess nutritional status in dairy cows. These profiles may help to confirm the diagnosis of sub clinical diseases, to be aware of possible causes of infertility in the herds, or to monitor improvement in herd animals. OBJECTIVES: This study investigated changes in the metabolic profile of high producing Holstein dairy cows from early lactation to close-up dry periods. The results of the current research can provide useful guidelines for management strategies during different physiological phases of high producing Holstein dairy cows. METHODS: Twenty-five multiparous high producing Holstein dairy cows were selected from a high producing industrial dairy farm. Cattle were divided into 5 equal groups of early, mid and late lactation and far-off and close-up dry cows. Blood samples were collected from all cows and sera were separated to evaluate glucose, insulin, β-hydroxybutyric acid (BHBA), non-esterified fatty acid (NEFA), cholesterol, triglyceride (TG), high, low and very low density lipoproteins (HDL-cholesterol, LDL-cholesterol and VLDL-cholesterol). RESULTS: The highest levels of insulin were detected in mid lactation and close-up dry periods. The changing patterns of BHBA and NEFA were significant and the highest levels of these biomarkers were detected in the early lactation group. The decreasing pattern of BHBA and NEFA were seen from early lactation to far-off dry cows. Significant elevations were seen in these biomarkers from far-off to close-up dry cows. CONCLUSIONS: The results of the present study show that metabolic biomarkers change in high producing Holstein dairy cows, under different physiological states. These changes are induced commonly by negative energy balance, lactogenesis and fetal growth in each state.

Keywords

Article Title [Persian]

مطالعه بر گلوکوز، انسولین، اسیدهای چرب غیر استریفیه، بتاهیدروکسی بوتیریک اسید و پروفایل چربی سرم در دورههای مختلف

Authors [Persian]

  • علی اصغر چالمه
  • مهرداد پورجعفر
  • سعید نظیفی
  • فروغ مومنی فر
  • محبوبه محمدی
گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شیراز، شیراز، ایران
Abstract [Persian]

زمینه مطالعه:  پروفایل متابولیک به منظور پیش بینی مسائل حوالی زایمان و وضعیت باروری، تشخیص بیماریهای متابولیک و دستیابی به شرایط تغذیه‌ای در گاوهای شیری مورد استفاده قرار گرفته است. این پروفایل ممکن است در تأیید تشخیص بیماریهای تحت بالینی، آگاهی از عوامل محتمل ناباروری در گله‌ها یا بهبود نظارت بر حیوانات گله یاری رسان باشد. هدف: مطالعه حاضر به منظور دستیابی به تصویری از تغییرات پروفایل متابولیک در جریان گردش خون گاوهای هلشتاین شیری پرتولید از ابتدای شیردهی تا انتهای دوره خشکی و آبستنی طراحی شد. نتایج این مطالعه می‌تواند الگویی مفید در اتخاذ استراتژی‌های مدیریتی در خلال دوره‌های مختلف فیزیولوژیک گاوهای هلشتاین شیری پرتولید فراهم کند. روش کار: تعداد 25 رأس گاو هلشتاین شیری پرتولید چند شکم زاییده از یک واحد گاوداری شیری صنعتی پرتولید انتخاب شدند. گاوها به 5 گروه مساوی شامل ابتدا، میانه و انتهای شیرواری و ابتدا و انتهای خشکی تقسیم شدند. نمونه‌های خون از تمام گاوها اخذ شد و سرم‌ها به منظور ارزیابی گلوکوز، انسولین، بتا-هیدروکسی بوتیریک اسید (BHBA)، اسید‌های چرب غیر استریفیه (NEFA)، کلسترول، تری گلیسیرید (TG)، لیپوپروتئین‌ها با چگالی بالا، کم و بسیار کم (HDL، LDL و VLDL) جداسازی شدند. نتایج: بالاترین غلظت انسولین در گروه‌های میانه شیرواری و انتهای خشکی مشاهده شد. الگوی تغیرات BHBA و NEFA در خلال دوره‌های مختلف معنی‌دار بود و بیشترین غلظت این بیومارکرها در گروه ابتدای شیرواری تشخیص داده شد. الگوی کاهشی غلظت BHBA و NEFA از ابتدای شیرواری تا ابتدای خشکی مشاهده شد. افزایش این بیومارکرها از گاوهای ابتدای خشکی تا انتهای خشکی معنی‌دار بود. نتیجه‌گیری‌نهایی: نتایج مطالعه حاضر نشان داد که بیومارکرهای متابولیک در خلال دوره‌های مختلف فیزیولوژیک گاوهای هلشتاین شیری پرتولید تغییر می‌کند. این تغییرات عمدتا به واسطه توازن منفی انرژی، شیرواری و رشد جنین در هر دوره القا می‌شوند.

Keywords [Persian]

  • الگوی تغییرات
  • شیرواری
  • رشد جنین
  • گاوهای شیری هلشتاین
  • پروفایل متابولیک

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Iranian Journal of Veterinary Medicine
Volume 9, Issue 3 - Serial Number 3
October 2015
Pages 171-178

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